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83 results about "Indole-3-acetic acid" patented technology

Indole-3-acetic acid (IAA, 3-IAA) is the most common, naturally occurring, plant hormone of the auxin class. It is the best known of the auxins, and has been the subject of extensive studies by plant physiologists. IAA is a derivative of indole, containing a carboxymethyl substituent. It is a colorless solid that is soluble in polar organic solvents.

Indole-3-acetic acid molecularly imprinted magnetic cellulose microsphere and preparation method and applications thereof

The invention discloses an indole-3-acetic acid molecularly imprinted magnetic cellulose microsphere and a preparation method and applications thereof, which relate to a nano magnetic cellulose molecularly imprinted microsphere and a preparation method and applications thereof. The invention is designed for solving the problem that an existing IAA (indole-3-acetic acid) detection method is complicated in step, time-consuming, quantitative in jamming and high in cost. The indole-3-acetic acid molecularly imprinted magnetic cellulose microsphere disclosed by the invention is prepared from an IAA, 4-VP, silanized beta-CD (beta-cyclodextrin), DMSO (dimethylsulfoxide), nano magnetic cellulose microsphere particles, a crosslinking agent, styrene, an initiator and water. The method comprises the following steps: 1, activating cellulose; 2, dissolving the cellulose; 3, preparing nano Fe3O4 magnetic liquid; 4, preparing nano magnetic cellulose microsphere particles; 5, silanizing beta-cyclodextrin; and 6, preparing the IAA molecularly imprinted magnetic cellulose microsphere. The indole-3-acetic acid molecularly imprinted magnetic cellulose microsphere disclosed by the invention, as an adsorbent, is used for quantitatively detecting the content of an indole-3-acetic acid in plant tissues.
Owner:NORTHEAST FORESTRY UNIVERSITY

Cutting propagation method for kmeria septentrionalis

The invention discloses a cutting propagation method for kmeria septentrionalis. The method includes the following steps that firstly, half-lignified branches are collected at September to October of every year and are trimmed into cutting slips, after the stem bases of the cutting slips are sterilized, soaking is performed in a rooting inductive agent for 2 h, taking out and drying are performed, and the rooting inductive agent is mainly a mixed aqueous solution of naphthyl acetic acid, indole acetic acid, aspirin and saccharose, wherein the concentration of the naphthyl acetic acid is 100-300 mg/L, the concentration of the indole acetic acid is 500-800 mg/L, the concentration of the aspirin is 50-100 mg/L and the concentration of the saccharose is 15-30 g/L; secondly, the cutting slips are inserted into a seedling bed medium and are immediately irrigated with the rooting inductive agent; and thirdly, a shade frame is erected on a seedling bed, the relative humidity in the seedling bed is controlled at 70-80%, the temperature is 26-28 DEG C, and the light shading rate is 50-60%. The cutting propagation method for the kmeria septentrionalis has the beneficial effects of being high in cutting rooting rate and survival rate, easy and convenient to operate, and the like.
Owner:GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS

Controlling plant pathogens and pests with applied or induced auxins

InactiveUS20050043177A1Inhibit growth of harmfulNegative effectBiocideAnimal repellantsMetaboliteFungal microorganisms
The present invention is directed to methods for inhibiting the growth of disease organisms, particularly fungi and bacteria, on plant tissues. The present invention is also directed to methods for inhibiting the infestation of plants by insects and larva, particularly sucking and chewing insects. These methods are achieved by applying an auxin or a plant growth regulator (PGR) which will effect the level of auxin in the plant tissue to the seeds or tubers of the plant prior to planting or to the roots, foliage, flowers or fruit of the plant after planting. The auxin or PGR is applied in an amount effective to inhibit growth of the disease organisms or insects, but in an amount insufficient to negatively effect growth of the plant tissues. The auxin may be applied as a natural auxin, synthetic auxin, auxin metabolite, auxin precursor, auxin derivative or a mixture thereof. The presently preferred auxin is indole-3-acetic acid (IAA). The auxin or PGR may be applied to the seeds, tubers or plant tissues. Seeds or tubers may be sprayed with or immersed in an aqueous solution containing the auxin or PGR. Conventional spraying and drip irrigation systems may be used to apply an aqueous solution containing an auxin or PGR to plant tissues. The auxin or PGR may also be applied to the plant tissues as a powder or may be encapsulated within a biologically compatible material to provide slow release to the roots of the plant. The plant tissues may be dusted with a powder, including the auxin or PGR. The encapsulated auxin may be placed in the root zone for uptake of the auxin or PGR by the roots.
Owner:STOLLER ENTERPRISES INC

Method for detecting indoleacetic acid and salicylic acid and electrochemical sensor used in method

The invention discloses a method for detecting indoleacetic acid and salicylic acid and an electrochemical sensor used in the method. The electrochemical sensor comprises a three-electrode system anda detection pool, wherein the three-electrode system comprises a working electrode, an Ag/AgCl reference electrode and a platinum wire counter electrode; the working electrode is a modified glassy carbon electrode; and the detection pool is internally filled with a detection base solution of a buffer solution. Electrochemical detection is carried out on IAA (Indole-3-Acetic Acid) and SA (SalicylicAcid) by utilizing a graphene hydrogel modified electrode; the problems that a general chromatographic method and an immunological detection method are complicated to operate, expensive in cost, highin sample size, high in time consumption, and the like, are solved; the real-time detection on the IAA and the SA is realized; the electrochemical sensor has the advantages of being convenient and quick, being high in detection sensitivity, having the detection limit of the IAA being as low as 0.677 mug/ml and the detection limit of the SA being as low as 0.569 mug/ml, and being low in needed sample amount, extremely low in detection cost, little in interference, and the like, and can be used for realizing the fast, simple, convenient and accurate quantitative detection on the IAA and the SA.
Owner:HEFEI UNIV OF TECH

Fertilizing method for improving stress tolerance and productivity of masson pine forest

The invention discloses a fertilizing method for improving stress tolerance and productivity of a masson pine forest. The method includes the following steps: when the masson pines are arrayed in a matrix or a square matrix, a pit is dug in the middle of a connecting line between every two adjacent trees in the transverse and the longitudinal directions; every two adjacent trees in the transverseand the longitudinal directions share one pit; and four pits are dug on the periphery of each masson pine; stress tolerance health protection mycorrhiza fungicide and organic fertilizer are mutually spaced and placed in the four pits on the periphery of each tree, and earthing is performed then. The stress tolerance health protection mycorrhiza fungicide is compounded and formulated by mycorrhizafungicide and naphthylacetic acid or indole-3-acetic acid, and the mycorrhiza fungicide is fermented by lactarius akahatsu and lactariushatsudake through solid fermentation stock culture; and biological organic fertilizer provides necessary nutrient for the growth of masson pines, and the stress tolerance health protection mycorrhiza fungicide facilitates the rootage and growth of the masson pines, so that the stress tolerance of the masson pines as well as the soil condition and micro ecology environment are improved, the survival rate and growth rate of the masson pines are enhanced, the rich water loss is reduced, and the competition for weeds below the masson pines and shrub for fertilizer is alleviated.
Owner:CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY

Preparation method and application of indole acetic acid (IAA) molecularly imprinted polymer (MIP)

The invention belongs to the field of analytical chemistry and particularly relates to a preparation method and application of indole acetic acid (IAA) molecularly imprinted polymer (MIP). The preparation method and the application are suitable for enriching and analyzing growth hormone plant samples. According to the method, IAA is used as template molecules, a functional monomer, a crosslinking agent and initiator are added so as to obtain a prepolymerization solution; and reaction is performed at the temperature of 60 DEG C for more than 8 hours, and the template modules are removed so as to obtain the IAAMIP. The IAAMIP can be used for a solid-phase micro-extraction (SPME) coating. The treated quartz fiber is inserted in the prepolymerization solution and is subjected to thermal-initiation polymerization; after reaction, the quartz fiber is taken out, aged and coated repeatedly; the template modules are cleaned completely; and finally the fiber is inserted into a micro-extraction device for subsequent extraction. The IAA-MIP and SPME (MISPME) coating is easy to prepare, controllable in thickness, high in selectivity and good in enrichment effect, and can be used repeatedly. An extraction head is combined with liquid chromatogram, so that two kinds of growth hormone in the plant samples can be efficiently extracted and separated.
Owner:FUZHOU UNIV

Screening method and application of Raoultella planticola strain SRPG-4 producing ACC (1-aminocyclopropane-1-carboxylic acid) deaminase activity

InactiveCN104450550AImprove germination rateReduce dosagePlant growth regulatorsBacteria1-Aminocyclopropane-1-carboxylic acidScreening method
The invention provides a screening method (comprising four steps of gathering, preliminary screening, secondary screening and identifying) and an application of a Raoultella planticola strain SRPG-4 producing 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity, wherein the strain is identified as Raoultella planticola SRPG-4 with a preservation number of CGMCC No.9392. The strain SRPG-4 obtained by the method provided by the invention can grow well in an ADF (Augmented Dickey-Fuller) culture medium, and the ACC specific enzymatic activity of the strain reaches 0.832+/-0.032U/mg. Under a salinization condition, the application of an SRPG-4 bacterium solution has an obvious effect of improving the germination rate, the planting percent and the biomass accumulation of cotton, and can adjust the output of an auxin (IAA namely indole-3-acetic acid) and relieving the generation of ethylene (Eth) and a cytokinin (ABA), thereby relieving salt stress. The strain screened by the method provided by the invention is derived from northwest arid areas, has strong adaptive capacity to environment and has advantages of stable application effect and the like, so that the strain used for developing a microorganism bacterium agent has a wide application prospect to solve the problem of soil salinization.
Owner:SHIHEZI UNIVERSITY +1

Sandy plant tissue culture medium, preparation method and application thereof

The invention discloses a sandy plant tissue culture medium, a preparation method thereof and an application thereof. The sandy plant tissue culture medium consists of river sand, nutrient solution, plant growth regulator, ascorbic acid, polyvinylpyrrolidone and fungicide, wherein with 1 liter as the standard, the nutrient solution accounts for 100-200 milliliters per liter, the various plant growth regulator such as 6-BA (6-Benzylaminopurine), NAA (1-naphthlcetic acid), IBA (indole-3-butytric acid) and IAA (indole-3-acetic acid) each account for 0.01-10.0 milliliters, the ascorbic acid accounts for 0.1-5.0 grams per liter, the polyvinylpyrrolidone accounts for 1-5 grams per liter, the fungicide accounts for 3-15 grams per liter, and the river sand accounts for the remaining volume, and the nutrient solution includes MS nutrient solution, sucrose, Zt, 6-BA and NAA. The preparation method of the sandy plant tissue culture medium includes various steps such as pretreatment, culture medium preparation, and post-formulation treatment. The sandy plant tissue culture medium can be widely applied to the tissue culture of of lianas, herbaceous plants and woody plants. The sandy plant tissue culture medium, the preparation method thereof and the application thereof have the advantages that with fine clean river sand as solid medium instead of agar, the cost of the culture medium is reduced; the river sand can be recycled for multiple times; accurately adjusting the pH of the nutrient solution, plant growth hormone and plant organic nutrient solution in plant tissue culture medium isunnecessary.
Owner:CHUXIONG NORMAL UNIV
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