Sandy plant tissue culture medium, preparation method and application thereof

Abstract

The invention discloses a sandy plant tissue culture medium, a preparation method thereof and an application thereof. The sandy plant tissue culture medium consists of river sand, nutrient solution, plant growth regulator, ascorbic acid, polyvinylpyrrolidone and fungicide, wherein with 1 liter as the standard, the nutrient solution accounts for 100-200 milliliters per liter, the various plant growth regulator such as 6-BA (6-Benzylaminopurine), NAA (1-naphthlcetic acid), IBA (indole-3-butytric acid) and IAA (indole-3-acetic acid) each account for 0.01-10.0 milliliters, the ascorbic acid accounts for 0.1-5.0 grams per liter, the polyvinylpyrrolidone accounts for 1-5 grams per liter, the fungicide accounts for 3-15 grams per liter, and the river sand accounts for the remaining volume, and the nutrient solution includes MS nutrient solution, sucrose, Zt, 6-BA and NAA. The preparation method of the sandy plant tissue culture medium includes various steps such as pretreatment, culture medium preparation, and post-formulation treatment. The sandy plant tissue culture medium can be widely applied to the tissue culture of of lianas, herbaceous plants and woody plants. The sandy plant tissue culture medium, the preparation method thereof and the application thereof have the advantages that with fine clean river sand as solid medium instead of agar, the cost of the culture medium is reduced; the river sand can be recycled for multiple times; accurately adjusting the pH of the nutrient solution, plant growth hormone and plant organic nutrient solution in plant tissue culture medium isunnecessary.

Description

technical field [0001] The invention belongs to the technical field of cultivation, and further belongs to the technical fields of plant tissue culture, plant tissue rapid propagation and modern soilless cultivation, and specifically relates to a sandy plant tissue culture medium and its preparation method and application. Background technique [0002] In plant tissue culture, there are three main types of media: solid, liquid, and semi-solid media. Wherein: liquid culture medium is liquid state, is made with various nutrient components adding water, or is made with the infusion juice of natural material (wort, bean sprouts juice etc.); Solid culture medium; semi-solid medium is in the form of a thin paste, which is made by adding 0.2 to 0.5% agar to the liquid medium. [0003] However, in the production and application of solid medium, it is necessary to add an appropriate amount of agar when preparing solid medium, and the cost of coagulants such as agar is high, and they...

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Problems solved by technology

[0003] However, in the production and application of solid medium, it is necessary to add an appropriate amount of agar when preparing solid medium, and the cost of coagulants such as agar is high, and they cannot be recycled for many times. After planting plant nutrient solutions, plant growth regulators, sugars, and plant organic nutrient solutions, the solid medium itself is prone to mildew; at the same time, when preparing solid medium, it is necessary to agar (this agar cannot be recycled for many times), a large amount of The pH of elemental nutrient solution, trace element nutrient solution, sugar solution, organic additives and hormones and other culture solutions should be accurately adjusted with acid or alkali. Bacteria are too hard or too soft after cooling, which is not conducive to plant tissue culture, and even leads to tissue culture failure

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