Sandy plant tissue culture medium, preparation method and application thereof

A plant tissue and culture medium preparation technology, applied in the field of cultivation, can solve the problems of high hardness, high cost, too soft, etc., and achieve the effect of reducing cost, reducing cost, and enhancing air permeability

Inactive Publication Date: 2018-08-21
CHUXIONG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, in the production and application of solid medium, it is necessary to add an appropriate amount of agar when preparing solid medium, and the cost of coagulants such as agar is high, and they cannot be recycled for many times. After planting plant nutrient solutions, plant growth regulators, sugars, and plant organic nutrient solutions, the solid medium itself is prone to mildew; at the same time, when preparing soli

Method used

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  • Sandy plant tissue culture medium, preparation method and application thereof
  • Sandy plant tissue culture medium, preparation method and application thereof
  • Sandy plant tissue culture medium, preparation method and application thereof

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preparation example Construction

[0032] The preparation method of the sandy plant tissue culture medium of the present invention comprises the steps of river sand pretreatment, sandy medium preparation and preparation post-treatment, specifically comprising:

[0033] A. Pre-treatment of river sand: wash the river sand with clear water, remove soil and sundries, dry or air-dry, and pass through a 10-50 mesh sieve to obtain fine river sand for later use;

[0034] B. Preparation of sand medium: add plant growth regulator, nutrient solution, ascorbic acid, polyvinylpyrrolidone and fungicide to the fine clean river sand treated in step A according to the proportion of raw materials, and mix evenly to obtain the target sand Plant tissue culture medium;

[0035] C. Post-preparation treatment: the target sandy plant tissue culture medium is divided into culture bottles, and the bottle caps are tightly closed, and then sterilized for later use.

[0036] The cleaning described in step A is rinsed with clean water, wit...

Embodiment 1

[0040] 1.1 Pre-processing

[0041] Take back the local fine river sand, wash it in clean water, wash away the soil and sundries, dry it in the sun or dry it, and sieve it through a 10-50 mesh sieve to obtain fine, dry river sand;

[0042] 1.2 Preparation and post-treatment of new sandy plant tissue culture medium

[0043]According to the formula of Dendrobium candidum cluster bud induction medium, take 1 liter of clean and spare river sand, add MS nutrient solution + NAA0.1mg / L +6-BA 2.0 mg / L + sucrose 25g / L + chlorothalonil 2 g / L + Carbendazim 5 g / L + Ascorbic acid 2.0g / L. Various Dendrobium officinale cluster bud induction medium formula culture solution is fully mixed with river sand, dry and wet moderately, and the target sandy plant tissue culture medium is divided into culture bottles, each bottle is 50cm 3 , tightly cap the bottle and place it in an autoclave for sterilization at a temperature of 121°C for 15-60 minutes. After sterilization, take it out and move it to...

Embodiment 2

[0047] 2.1 Pre-processing

[0048] Take back the local fine river sand, wash it in clean water, wash away the soil and sundries, dry it in the sun or dry it, and sieve it through a 10-50 mesh sieve to obtain fine, dry river sand;

[0049] 2.2 Preparation and post-treatment of sandy plant tissue culture medium

[0050] According to the formula of Dendrobium officinale adventitious bud induction medium, take 1 liter of clean and spare river sand, add MS nutrient solution + NAA1.0mg / L +6-BA 0.1 mg / L + sucrose 25g / L + chlorothalonil 2 g / L + Carbendazim 5 g / L + Ascorbic acid 2.0g / L. Various dendrobium officinale adventitious root induction medium formula culture solution is fully mixed with river sand, dry and wet moderately, and the target sandy plant tissue culture medium is divided into culture bottles, each bottle is 50cm 3 , tightly cap the bottle and place it in an autoclave for sterilization at a temperature of 121°C for 15-60 minutes. After sterilization, take it out and ...

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Abstract

The invention discloses a sandy plant tissue culture medium, a preparation method thereof and an application thereof. The sandy plant tissue culture medium consists of river sand, nutrient solution, plant growth regulator, ascorbic acid, polyvinylpyrrolidone and fungicide, wherein with 1 liter as the standard, the nutrient solution accounts for 100-200 milliliters per liter, the various plant growth regulator such as 6-BA (6-Benzylaminopurine), NAA (1-naphthlcetic acid), IBA (indole-3-butytric acid) and IAA (indole-3-acetic acid) each account for 0.01-10.0 milliliters, the ascorbic acid accounts for 0.1-5.0 grams per liter, the polyvinylpyrrolidone accounts for 1-5 grams per liter, the fungicide accounts for 3-15 grams per liter, and the river sand accounts for the remaining volume, and the nutrient solution includes MS nutrient solution, sucrose, Zt, 6-BA and NAA. The preparation method of the sandy plant tissue culture medium includes various steps such as pretreatment, culture medium preparation, and post-formulation treatment. The sandy plant tissue culture medium can be widely applied to the tissue culture of of lianas, herbaceous plants and woody plants. The sandy plant tissue culture medium, the preparation method thereof and the application thereof have the advantages that with fine clean river sand as solid medium instead of agar, the cost of the culture medium is reduced; the river sand can be recycled for multiple times; accurately adjusting the pH of the nutrient solution, plant growth hormone and plant organic nutrient solution in plant tissue culture medium isunnecessary.

Description

technical field [0001] The invention belongs to the technical field of cultivation, and further belongs to the technical fields of plant tissue culture, plant tissue rapid propagation and modern soilless cultivation, and specifically relates to a sandy plant tissue culture medium and its preparation method and application. Background technique [0002] In plant tissue culture, there are three main types of media: solid, liquid, and semi-solid media. Wherein: liquid culture medium is liquid state, is made with various nutrient components adding water, or is made with the infusion juice of natural material (wort, bean sprouts juice etc.); Solid culture medium; semi-solid medium is in the form of a thin paste, which is made by adding 0.2 to 0.5% agar to the liquid medium. [0003] However, in the production and application of solid medium, it is necessary to add an appropriate amount of agar when preparing solid medium, and the cost of coagulants such as agar is high, and they...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 李国树徐成东
Owner CHUXIONG NORMAL UNIV
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