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Tissue culture and rapid propagation method of solanaceae lycium brevipes

A tissue culture and Lycium barbarum technology is applied in the field of plant tissue culture to achieve the effects of light substrate quality, cost reduction and high transplant survival rate

Active Publication Date: 2014-10-15
GANSU AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of this invention is to provide a kind of Solanaceae Lycium genus Brachyphyllum tissue culture and rapid propagation method, to solve the breeding problem of introducing Lycium barbarum

Method used

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  • Tissue culture and rapid propagation method of solanaceae lycium brevipes
  • Tissue culture and rapid propagation method of solanaceae lycium brevipes
  • Tissue culture and rapid propagation method of solanaceae lycium brevipes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A. Explant treatment

[0031] Collect the young stems of Lycium brevipes (Lycium brevipes) of the family Solanaceae as explants, cut off the leaves and leave a small amount of petioles, cut each 2-3 buds into sections, wash the stems with washing powder water to remove stains, and then Rinse with tap water for 5-10 minutes. Then put it into an ultra-clean workbench for disinfection, cut off both ends of the stem after disinfection, and put it in a petri dish for later use.

[0032] B. Primary culture

[0033] 30 bottles were inoculated for each treatment, and the growth status of the explants was recorded every 7 days after inoculation.

[0034] Based on MS medium, add 0.05mg / L NAA, 3% sucrose and 4g agar, and adjust the pH of the medium to 5.8 with NaOH or HCl. The culture conditions are 3000 lx full light for 24 hours, a temperature of 22°C, and a humidity of 70%RH. The proportion of adventitious bud growth reached 86.7%.

[0035] C. Subculture

[0036] The cult...

Embodiment 2

[0042] Transplant the products after seedling hardening in Example 1: the most suitable seedling hardening and transplanting matrix is ​​mixed with 1:1 peat and vermiculite for the best effect, and the matrix is ​​sterilized at high temperature before transplanting. After cooling, put it in pots and prepare for transplanting. Before transplanting, pour the substrate with 0.1% carbendazim. When transplanting, take the refined seedlings out of the Erlenmeyer flask and put them in water to wash the medium on the roots. , Soak the roots in a mixed solution of 0.1% carbendazim and 0.1 g / L NAA for 10 minutes. Transplant the seedlings into flower pots with substrate, water thoroughly and cover with film.

[0043] In the initial stage of transplanting wolfberry tissue culture seedlings, because the seedlings are young, sunshade nets must be installed, and the light intensity should be about 30% of that under full sunlight.

[0044] In the first two days, shading, sealing and moisturi...

Embodiment 3

[0045] Example 3 The difference from Example 1 is that the specific scheme of disinfection in step A is as follows: first sterilize with 75% alcohol shaking for 5 seconds, then sterilize with 0.1% mercuric chloride solution for 6 minutes, and finally wash with sterile water for 3-4 times.

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Abstract

The invention discloses a tissue culture and rapid propagation method of solanaceae lycium brevipes, and aims to solve the problem of propagation of introduced lycium barbarum. The method comprises the following steps: explant treatment, primary culture, subculture, rooting culture, hardening seedling and transplanting, the advantages of the method are as follows: A, only 6-benzyl amino adenine and indole-3-acetic acid are used for mixing during subculture of the solanaceae lycium brevipes, the effect of hormone accumulation on multi times of subculture can be greatly reduced, the cost is also reduced; B, peat and vermiculite in the ratio of 1:1 are used as a hardening seedling and transplanting matrix, the matrix is simple in ratio, light in mass, good in performance in transplanting test, and higher in transplanting survival rate; and C, the tissue culture and rapid propagation method plays a great role on late improvement and application of local Chinese wolfberry.

Description

technical field [0001] The invention belongs to the field of plant tissue culture, and in particular relates to a method for tissue culture and rapid propagation of Lycium brevipes of the genus Lycium genus Solanaceae. Background technique [0002] Lycium brevipes (Lycium brevipes) is a plant of the Solanaceae Lycium genus introduced from the United States. It is mainly distributed in western Mexico and the Sonoran Desert in California. It is mainly produced in deserts and coastal areas. Northwest of the United States is also used for greening; its evolutionary degree is higher than that of Chinese wolfberry. In terms of reproductive evolution, it has evolved from hermaphroditism and self-incompatible diploid to self-compatible polyploid stage, while my country is still In the self-incompatible diploid evolution stage. The introduction of this kind of wolfberry has great significance for the improvement of germplasm resources of Chinese wolfberry. The primary problem faced a...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 李捷冯丽丹王有科张宝琳张广忠蔡国军
Owner GANSU AGRI UNIV
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