Tissue culture and rapid propagation method of lycium exsertum
A tissue culture, rapid technology, applied in the field of plant tissue culture, can solve the problems of expansion and reproduction, and achieve the effect of improving survival rate, reducing cost and good effect
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Embodiment 1
[0032] A. Explant treatment
[0033] Collect the young stems of Lyciumexsertum as explants, cut off the leaves and leave a small amount of petioles, cut each 2-3 buds into a section, wash the stems with washing powder to remove stains, and then rinse them with tap water for 5-5 10min. Then put it into an ultra-clean workbench for disinfection, cut off both ends of the stem after disinfection, and put it in a petri dish for later use.
[0034] B. Primary culture
[0035] 30 bottles were inoculated for each treatment, and the growth status of the explants was recorded every 7 days after inoculation.
[0036] Based on MS medium, add 0.05mg / L NAA, 3% sucrose and 4g agar, and adjust the pH of the medium to 5.8 with NaOH or HCl. The culture conditions are 3000 lx full light for 24 hours, a temperature of 25°C, and a humidity of 70%RH. The proportion of adventitious bud growth reached 93.3%.
[0037] C. Subculture
[0038] The culture materials are single seedlings or clump bud...
Embodiment 2
[0044] The difference from Example 1 is that the steps of rooting culture are different: use the leaves of the cultured plants in step B as material to carry out cluster bud proliferation culture, take 1 / 2MS medium as the base medium, add 1.0mg / L of 6-benzylaminoadenine, 0.5 mg / L indole butyric acid (IBA); the culture condition is to first culture at 25°C in dark for 7 days, then at 25°C, with a light intensity of 3000lx full light, and a humidity of 70%RH until cluster buds grow.
Embodiment 3
[0046] The difference from Example 1 is that the rooting culture steps are different: use the leaves of the cultured plants in step C as material to carry out cluster bud proliferation culture, take 1 / 2MS medium as the base medium, add 1.0mg / L of 6-benzylaminoadenine, 0.5 mg / L indole butyric acid (IBA); the culture condition is to first culture at 25°C in dark for 7 days, then at 25°C, with a light intensity of 3000lx full light, and a humidity of 70%RH until cluster buds grow. Under this condition, the multiplication coefficient of cluster buds is the highest, the growth is the most vigorous, and the multiplication coefficient can reach more than 5.0.
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