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Scindapsus aureus rooting medium and scindapsus aureus rooting culture method

A rooting medium and rooting culture technology, which is applied in the field of plant tissue culture, can solve problems affecting the research of pothos, and achieve the effects of high transplanting survival rate, promotion of rooting, and good root development

Inactive Publication Date: 2017-05-10
东方上彩现代农业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] But at present, there is no medium suitable for the tissue culture of pothos, which seriously affects the research on the classic material of pothos

Method used

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  • Scindapsus aureus rooting medium and scindapsus aureus rooting culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] A method for cultivating roots of pothos, comprising the following steps:

[0059] (1) Preparation of rooting medium: Dissolve naphthaleneacetic acid, indole-3-acetic acid, mashed potatoes, sucrose and agar powder in MS basic medium, and cool to room temperature after autoclaving for use; wherein the dosage of each component MS minimal medium 1L, naphthalene acetic acid 0.05 mg, indole-3-acetic acid 0.01 mg, mashed potatoes 30 g, sucrose 30 g, agar powder 7 g.

[0060] (2) Inoculation: On the ultra-clean workbench and under aseptic conditions, cut the bushy shoots of the pothos into individual plants, cut off the base tissue and some leaves, and inoculate it into the rooting medium prepared in step (1) middle.

[0061] (3) rooting culture: under the cultivation conditions that the clustered sprouts of the inoculated pothos are aseptic, the light intensity is 1500~20001x, the light time is 12h / d, the temperature is 25~28°C, and the humidity is 50~65%. After cultivating...

Embodiment 2

[0063] A method for cultivating roots of pothos, comprising the following steps:

[0064] (1) Preparation of rooting medium: Dissolve naphthaleneacetic acid, indole-3-acetic acid, mashed potatoes, sucrose and agar powder in MS basic medium, and cool to room temperature after autoclaving for use; wherein the dosage of each component 1 L of MS basic medium, 0.06 mg of naphthaleneacetic acid, 0.02 mg of indole-3-acetic acid, 45 g of mashed potatoes, 30 g of sucrose, and 7 g of agar powder.

[0065] (2) Inoculation: On the ultra-clean workbench and under aseptic conditions, cut the bushy shoots of the pothos into individual plants, cut off the base tissue and some leaves, and inoculate it into the rooting medium prepared in step (1) middle.

[0066] (3) rooting culture: under the cultivation conditions that the clustered sprouts of the inoculated pothos are aseptic, the light intensity is 1500~20001x, the light time is 12h / d, the temperature is 25~28°C, and the humidity is 50~65%...

Embodiment 3

[0068] A method for cultivating roots of pothos, comprising the following steps:

[0069] (1) Preparation of rooting medium: Dissolve naphthaleneacetic acid, indole-3-acetic acid, mashed potatoes, sucrose and agar powder in MS basic medium, and cool to room temperature after autoclaving for use; wherein the dosage of each component 1 L of MS basic medium, 0.08 mg of naphthaleneacetic acid, 0.03 mg of indole-3-acetic acid, 50 g of mashed potatoes, 30 g of sucrose, and 7 g of agar powder.

[0070] (2) Inoculation: On the ultra-clean workbench and under aseptic conditions, cut the bushy shoots of the pothos into individual plants, cut off the base tissue and some leaves, and inoculate it into the rooting medium prepared in step (1) middle.

[0071] (3) rooting culture: under the cultivation conditions that the clustered sprouts of the inoculated pothos are aseptic, the light intensity is 1500~20001x, the light time is 12h / d, the temperature is 25~28°C, and the humidity is 50~65%...

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Abstract

The invention discloses a scindapsus aureus rooting medium which is prepared from MS minimal medium, naphthylacetic acid, indole-3-acetic acid, mashed potato, saccharose and agar powder. According to the scindapsus aureus rooting medium and the scindapsus aureus rooting culture method, the MS culture medium is used as the minimal medium and is combined with the specific concentration of naphthylacetic acid, an indole-3-acetic acid plant growth regulator, the mashed potato, the saccharose and the agar powder; thus, the rooting medium can effectively promote the scindapsus aureus crowd shoots to root. When the rooting medium is used for performing rooting culture of the scindapsus aureus crow shoots, the rooting rate can reach 96% or more, the root systems grow well, and the transplanting survival rate is high; thus, the method provides technical support for industrialization, popularization and application of scindapsus aureus tissue culture.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a rooting culture medium for pothos and a rooting culture method for pothos. Background technique [0002] Green dill (scientific name: Epipremnum aureum), belonging to the Araceae genus Kylin, a large evergreen vine, likes a warm and humid environment, grows in tropical areas, often climbs and grows on rocks and tree trunks in the rainforest, and has strong entanglement and strong air. The roots are well developed and can be grown hydroponically. [0003] Pollen is one of the very good indoor decorative plants, climbing vines and foliage flowers. The radish stem is thin and soft, and the leaves are delicate and delicate. Set pots high on the top of the furniture cabinet, let the vines hang down calmly, or hang them in a circle after the vines hang too long, like emerald emerald reliefs. This not only makes full use of the space, purifies the air, but also adds gr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005A01H4/008
Inventor 王健刘坤申亮郑德助卢迪浩包宇飞李健施金玉
Owner 东方上彩现代农业有限公司
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