Method for detecting indoleacetic acid and salicylic acid and electrochemical sensor used in method

A sensor and electrochemical technology, applied in the field of a method and its electrochemical sensor, can solve the problems of expensive modified electrode materials, few types of functional monomers, and high cost

Inactive Publication Date: 2019-03-26
HEFEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Electrochemical sensors currently used to detect IAA and SA are multi-modified electrodes, such as Suriyanarayanan S, Mandal S, Ramanujam K, et al. Electrochemically synthesized molecularly imprinted polythiophene nanostructures as recognition elements for an aspirin-chemosensor[J].Sensors&Actuators B Chemical, 2017, 253, using a sacrificial alumina template on a gold-coated quartz resonator to incorporate a molecularly imprinted polymer (MIP) composed of functional monomers 3-thiophene boronic acid (3-TBA) and 3-thiophene acetic acid (3-TAA) as an electrode, Good results have been achieved in the detection of SA, using methods that can specifically recognize imprinted molecules or molecules with similar str

Method used

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  • Method for detecting indoleacetic acid and salicylic acid and electrochemical sensor used in method
  • Method for detecting indoleacetic acid and salicylic acid and electrochemical sensor used in method
  • Method for detecting indoleacetic acid and salicylic acid and electrochemical sensor used in method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: A method for simultaneous detection of indole-3-acetic acid and salicylic acid and the electrochemical sensor used therefor

[0026] (1) Electrode pretreatment: use 1.0, 0.3 and 0.05μm Al respectively 2 o 3 The powder was polished to a mirror surface, and then ultrasonically cleaned with absolute ethanol and double distilled water. 2 Blow dry and set aside.

[0027] (2) Preparation of buffer: prepare 0.1M NaH respectively 2 PO 4 solution and 0.1M H 3 PO 4 solution with 0.1M H 3 PO 4 Solution adjusted to 0.1M NaH 2 PO 4 The pH of the solution was brought to 2.5.

[0028] (3) Preparation of graphene hydrogels (GHs):

[0029] 10 mL of GO dispersion (3 mg / mL) was sonicated for 20 min, 66 mg of sodium ascorbate was added under sonication, and NaOH or H 2 SO 4 solution to adjust the pH of the GO dispersion to 3.47, and the mixture was sealed in an autoclave and heated at 90 °C for 3 h to form GH.

[0030] (4) Preparation of modified glassy carbon elec...

Embodiment 2

[0039] Example 2: A method for simultaneous detection of indole-3-acetic acid and salicylic acid and the electrochemical sensor used therefor

[0040] (1) Electrode pretreatment: use 1.0, 0.3 and 0.05μm Al respectively 2 o 3 The powder was polished to a mirror surface, and then ultrasonically cleaned with absolute ethanol and double distilled water. 2 Blow dry and set aside.

[0041] (2) Preparation of buffer: prepare 0.1M NaH respectively 2 PO 4 solution and 0.1M H 3 PO 4 solution with 0.1M H 3 PO 4 Solution adjusted to 0.1M NaH 2 PO 4 The pH of the solution was brought to 2.5.

[0042] (3) Preparation of graphene hydrogels (GHs):

[0043] 10 mL of GO dispersion (3 mg / mL) was sonicated for 20 min, 66 mg of sodium ascorbate was added under sonication, and NaOH or H 2 SO 4 solution to adjust the pH of the GO dispersion to 3.47, and the mixture was sealed in an autoclave and heated at 90 °C for 3 h to form GH.

[0044] (4) Preparation of modified glassy carbon elec...

Embodiment 3

[0053] Example 3: A method for simultaneous detection of indole-3-acetic acid and salicylic acid and the electrochemical sensor used therefor

[0054] (1) Electrode pretreatment: use 1.0, 0.3 and 0.05μm Al respectively 2 o 3 The powder was polished to a mirror surface, and then ultrasonically cleaned with absolute ethanol and double distilled water. 2 Blow dry and set aside.

[0055] (2) Preparation of buffer: prepare 0.1M NaH respectively 2 PO 4 solution and 0.1M H 3 PO 4 solution with 0.1M H 3 PO 4 Solution adjusted to 0.1M NaH 2 PO 4 The pH of the solution was brought to 2.5.

[0056] (3) Preparation of graphene hydrogels (GHs):

[0057] 10 mL of GO dispersion (3 mg / mL) was sonicated for 20 min, 66 mg of sodium ascorbate was added under sonication, and NaOH or H 2 SO 4 solution to adjust the pH of the GO dispersion to 3.47, and the mixture was sealed in an autoclave and heated at 90 °C for 3 h to form GH.

[0058] (4) Preparation of modified glassy carbon elec...

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PUM

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Abstract

The invention discloses a method for detecting indoleacetic acid and salicylic acid and an electrochemical sensor used in the method. The electrochemical sensor comprises a three-electrode system anda detection pool, wherein the three-electrode system comprises a working electrode, an Ag/AgCl reference electrode and a platinum wire counter electrode; the working electrode is a modified glassy carbon electrode; and the detection pool is internally filled with a detection base solution of a buffer solution. Electrochemical detection is carried out on IAA (Indole-3-Acetic Acid) and SA (SalicylicAcid) by utilizing a graphene hydrogel modified electrode; the problems that a general chromatographic method and an immunological detection method are complicated to operate, expensive in cost, highin sample size, high in time consumption, and the like, are solved; the real-time detection on the IAA and the SA is realized; the electrochemical sensor has the advantages of being convenient and quick, being high in detection sensitivity, having the detection limit of the IAA being as low as 0.677 mug/ml and the detection limit of the SA being as low as 0.569 mug/ml, and being low in needed sample amount, extremely low in detection cost, little in interference, and the like, and can be used for realizing the fast, simple, convenient and accurate quantitative detection on the IAA and the SA.

Description

technical field [0001] The invention belongs to the technical field of food detection and electrochemical sensors, and in particular relates to a method for detecting indole acetic acid and salicylic acid and an electrochemical sensor used therefor. Background technique [0002] Plant-produced or synthetic plant growth regulator indole-3-acetic acid (IAA) is a small organic molecule that alters plant growth and development. IAA stimulates the growth process, similar to many plant growth regulators such as abscisic acid (ABA), Jasmonic acid (JA) has been widely used in many countries such as Australia, Japan, China, and India, which has drawn more attention to the toxicity and residue issues of its edible plants. Legislative bodies have pointed out maximum residue limits for the protection of health, and reducing the concentration of plant genetic resources residues in commodities as much as possible is one of the most important quality standards in market monitoring and inte...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/48
CPCG01N27/308G01N27/48
Inventor 操小栋朱雪婷叶永康
Owner HEFEI UNIV OF TECH
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