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Preparation method and application of indole acetic acid (IAA) molecularly imprinted polymer (MIP)

A molecular imprinting, indole acetic acid technology, applied in the field of analytical chemistry, can solve the problems of limited specificity and high price, and achieve the effects of enhanced selectivity, low cost and simple fiber processing

Inactive Publication Date: 2012-10-03
FUZHOU UNIV
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Problems solved by technology

Liu et al. used SPME-HPLC combined technology to determine four plant hormones in plantain xylem stream, but this method involves commercial SPME extraction head, which is expensive and has limited specificity
However, using IAA as a template molecule to prepare a fibrous MISPME coating for the analysis of auxin has not been reported so far.

Method used

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  • Preparation method and application of indole acetic acid (IAA) molecularly imprinted polymer (MIP)
  • Preparation method and application of indole acetic acid (IAA) molecularly imprinted polymer (MIP)
  • Preparation method and application of indole acetic acid (IAA) molecularly imprinted polymer (MIP)

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Embodiment Construction

[0035] Prepare IAA-MISPME coating by content of the present invention:

[0036] a) Quartz fiber pretreatment: Take a quartz fiber about 20 cm long, soak one end in acetone for 5 minutes, peel off the polyimide coating on the outer layer, and wash with secondary water, 1.0 mol / L NaOH, and secondary water successively. , soaked in 1 mol / L HCl, washed, dried, put the quartz fiber into 10% (v / v) γ-MAPS acetone solution for silanization treatment for 2 h, rinsed with methanol after taking it out, blown dry with nitrogen, and set aside .

[0037] b) Preparation of pre-polymerization solution: use IAA as a template molecule, add an appropriate amount of 4-vinylpyridine (functional monomer) and acetonitrile (porogen) and mix well, leave at room temperature for 12 h, then add trimethylolpropane trimethacrylic acid Esters (crosslinking agent) and azobisisobutyronitrile (initiator), ultrasonically mixed, nitrogen and oxygen removed to obtain a pre-polymerization solution.

[0038] c) P...

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Abstract

The invention belongs to the field of analytical chemistry and particularly relates to a preparation method and application of indole acetic acid (IAA) molecularly imprinted polymer (MIP). The preparation method and the application are suitable for enriching and analyzing growth hormone plant samples. According to the method, IAA is used as template molecules, a functional monomer, a crosslinking agent and initiator are added so as to obtain a prepolymerization solution; and reaction is performed at the temperature of 60 DEG C for more than 8 hours, and the template modules are removed so as to obtain the IAAMIP. The IAAMIP can be used for a solid-phase micro-extraction (SPME) coating. The treated quartz fiber is inserted in the prepolymerization solution and is subjected to thermal-initiation polymerization; after reaction, the quartz fiber is taken out, aged and coated repeatedly; the template modules are cleaned completely; and finally the fiber is inserted into a micro-extraction device for subsequent extraction. The IAA-MIP and SPME (MISPME) coating is easy to prepare, controllable in thickness, high in selectivity and good in enrichment effect, and can be used repeatedly. An extraction head is combined with liquid chromatogram, so that two kinds of growth hormone in the plant samples can be efficiently extracted and separated.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and more specifically relates to a preparation method and application of an indole acetic acid molecularly imprinted polymer, which is suitable for the enrichment and analysis of auxin in plant samples. Background technique [0002] Auxins are a class of plant hormones necessary for plant growth and development. Common auxins are indole acetic acid (IAA), indole butyric acid, 4-chloroindole acetic acid, indole propionic acid (IPA) and so on. They all use indole as the parent ring, and are a class of compounds with similar structures. In actual production, auxin has physiological functions such as regulating plant apical dominance, promoting tissue and organ maturation and aging, so accurate determination of its content can more effectively understand the mechanism of auxin. [0003] Solid phase microextraction (SPME) is a pretreatment technology developed on the basis of solid phase extracti...

Claims

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Application Information

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IPC IPC(8): C08J9/28C08F226/06C08F222/14C08F2/44D06M15/356G01N30/08
Inventor 张兰卢巧梅范良彪蔡惠坚陈国南
Owner FUZHOU UNIV
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