31 results about "Theobromine" patented technology

The invention discloses a preparation method of burdock powder and chocolate. The preparation method of the burdock powder comprises the following steps: baking burdock at the temperature of 110-130 DEG C until the burdock generates scorched cocoa fragrance, so as to obtain a first material; and crushing the first material to obtain powder. The burdock powder prepared by adopting the method has a relatively high polysaccharide component retention rate and relatively strong scorch aroma and cocoa scorch aroma, and can be used as an auxiliary material of chocolate to replace caffeine and theobromine in traditional chocolate, so that the chocolate not only has remarkable health benefits, but also has good flavor and palatability.

The present invention discloses a method for utilizing [C 12 mim]Br / STC[4] aqueous two-phase system for selective extraction of theobromine. 12 The solution containing theobromine to be extracted was added to the aqueous biphasic system of mim]Br / 4 wt% STC[4]. After mixing, the mixture was allowed to stand and centrifuged to completely separate the two phases. The distribution coefficients of Van, Syr, MA, Trp, and Cep were less than 1, namely 0.23, 0.24, 0.43, 0.44, and 0.56, respectively. The distribution coefficients of Tyr, Phe, Theo, and TB were greater than 1, namely 2.78, 2.07, and 12.45, respectively. The selectivity of TB for Syr, Van, MA, Trp, Cep, Tyr, Theo, and Phe were 51.88, 54.13, 28.95, 28.30, 22.23, 4.48, 6.01, and 5.00, respectively, achieving efficient and selective extraction of theobromine.

The invention discloses a method for rapidly and quantitatively analyzing caffeine, theophylline and theobromine in a beverage. The method comprises the following steps: mixing a solution containing caffeine, theophylline and theobromine with an MALDI matrix, and carrying out qualitative and quantitative analysis on a target object through MALDI primary mass spectrometry detection or combination of MALDI primary and secondary mass spectrometry detection. The quantitative analysis method provided by the invention is simple, convenient and rapid to operate, and the whole experimental process can be completed within 2 minutes. According to the method, absolute quantitative analysis of the pair of isomers of theophylline and theobromine can be realized, and the rapid and high-throughput detection requirements of caffeine, theophylline and theobromine in a beverage sample can be met.

The invention belongs to the field of cocoa extract tobacco flavors, and particularly relates to a preparation method and application of a high-theobromine cocoa extract and a style-enhanced cocoa extract. The high-theobromine cocoa extract is used for tobacco perfuming independently or after being blended with the cocoa extract tobacco flavor, so that the sensory effect of the cocoa extract tobacco flavor is improved; the preparation method of the high-theobromine cocoa extract comprises the following steps: preparing a cocoa powder water extract or a cocoa extract water diluent, and enriching theobromine in the cocoa extract by using macroporous adsorption resin column chromatography. Compared with pure theobromine, the high-theobromine cocoa extract has no obvious influence on the sensory quality of cigarettes, and compared with untreated cocoa extracts, the high-theobromine cocoa extract has the advantages that the aroma, richness, stimulation reduction, offensive odor covering, characteristic aroma intensity and the like are improved to different degrees; especially, the style-enhanced cocoa extract obtained by blending the high-theobromine cocoa extract and the untreated cocoa extract has a better effect.

This application relates to the technical field of biomedical detection, and specifically discloses a method for detecting the content of the genotoxic impurity 6-chloro-2-hexanone in pentoxifylline raw materials and injection solutions. The detection method disclosed in this application uses gas chromatography-mass spectrometry (GC-MS) to detect samples; the gas chromatography parameters are: injection port temperature 190 - 210 °C; temperature programming: initial temperature 75 - 85 °C, heated to 150 - 170 °C at a rate of 15 - 25 °C / min, held for 1.5 - 2.5 min, then heated to 210 - 230 °C at a rate of 55 - 65 °C / min, held for 1.5 - 2.5 min; the mass spectrometry parameters are: solvent delay 3 - 4 min; transfer line temperature 240 - 260 °C; quadrupole temperature 140 - 160 °C; ion source temperature 240 - 260 °C. The method of this application is simple to operate, has a fast detection speed, and has the advantages of high sensitivity, high precision, and high repeatability.

For the purpose of determination for the selection of intravesical BCG instillation therapy, the present invention comprises measuring 2-ethylhydracrylic acid-2, arabinonic acid-2, carnitine, lauric acid, myristic acid, octanoylcarnitine, paraxanthine, S-methylcysteine, S-methylcysteine-S-oxide, SDMA, theobromine, indole-3-acetic acid, pentanoic acid, 1-methyl-4-imidazoleacetic acid, ADMA, alanine, argininosuccinic acid, choline, citrulline, creatinine, guanidoacetic acid, lysine, methionine sulfoxide, myristic acid, N-(1-deoxy-1-fructosyl)leucine, N-acetylneuraminic acid, N6,N6,N6-trimethyllysine, propionylcarnitine, threonine, gamma-glutamyl-glycine, gamma-glutamyl-tyrosine, and gamma-glutamyl-valine in blood, serum, or plasma.

The present application relates to the field of tea tree biotechnology, in particular to Fangcheng tea low caffeine related molecular markers based on liquid chip and application thereof. The present application detects 16 different types of tea tree resources including Fangcheng tea and related alkaloid index content by using the liquid chip developed in the early stage, identifies 6 sense SNPs distributed on the CfTCS1 gene of Fangcheng tea caffeine synthesis enzyme, and uses the 6 mutations to accurately, efficiently and low-cost identify Fangcheng tea germplasm resources. The mutations are closely related to the caffeine and theobromine content of tea tree, and the genotypes can be used to quickly screen low caffeine and high theobromine germplasm resources. The results of the specific embodiments of the present application show that when the genotypes of the 6 SNP molecular markers are AA / AA / TT / GG / TT / GG in turn, the tea tree resources correspond to Fangcheng tea germplasm resources with high theobromine and low caffeine content.

The invention relates to the use of an extract of Phaeodactylum tricornutum alone or in combination with an extract chosen from a guarana (Paullinia cupana) extract, a ginseng (Panax ginseng) extract, a Ginkgo biloba extract, a Nelumbo nucifera extract, a Centella asiatica extract, a Piper nigrum extract, a Bacopa monnieri extract or any combination thereof and / or a compound chosen from arginine, creatine, caffeine, theanine, vitamin C, vitamin E, vitamin B, theophylline, paraxanthine, theobromine, rosmarinic acid, ellagic acid, cinnamaldehyde or any combination thereof, for maintaining and / or increasing data processing speed and / or concentration in healthy mammals. The combination of the extract of P. tricornutum and one of these extracts or one of these compounds is also described. The invention also relates to a composition comprising the combination according to the invention.

The invention relates to the technical field of tea tree biology, in particular to a Qingcheng tea low-caffeine related molecular marker based on a liquid phase chip and application of the molecular marker. According to the invention, 16 different types of tea tree resources including the defensive tea are detected by using a liquid phase chip developed at an early stage, and the content of related alkaloid indexes is detected at the same time, so that 6 sense SNP mutations distributed on the defensive tea caffeine synthetase CfTCS1 gene are identified, and the germplasm resources of the defensive tea can be accurately and efficiently identified at low cost by using the 6 mutations, so that the method has a wide application prospect. The mutations are closely related to the caffeine content and the theobromine content of the tea trees, and low-caffeine and high-theobromine germplasm resources can be rapidly screened according to the genotypes of the tea trees. The result of the specific embodiment of the invention shows that when the tea tree resource of which the genotypes of the six SNP molecular markers are AA / AA / TT / GG / TT / GG in sequence corresponds to the Guangcheng tea germplasm resource with high theobromine-low caffeine content.

The invention relates to the technical field of crystallization control of chemical bulk drug intermediates, and particularly provides a crystallization method of a pentoxifylline intermediate, which comprises the following steps: adding an acetic acid solution into a 3, 7-dimethylxanthine neutralization reaction solution at 80-85 DEG C until crystals are separated out, carrying out heat preservation and stirring for a first preset time, filtering, washing, and drying to obtain the pentoxifylline intermediate. And continuously adding an acetic acid solution to adjust the pH value of the system to be neutral, carrying out heat preservation stirring for a second preset time, slowly cooling to 45-55 DEG C, carrying out heat preservation stirring for a third preset time, and carrying out solid-liquid separation to obtain the pentoxifylline intermediate. According to the crystallization method provided by the invention, the problems of difficulty in centrifugal separation, easiness in blockage of filter cloth and redness of a wet product caused by high viscosity of a solid-liquid mixture in an existing staged cooling and crystal growing process are solved, the yield of the prepared 3, 7-dimethylxanthine product reaches 65% or above, the purity can reach 99.9% or above, the production efficiency is improved, and the crystallization method is suitable for large-scale industrial production.

The invention belongs to the technical field of medicines, and particularly relates to combined application of nicotine and theobromine in relieving bipolar affective disorder as well as a product and a preparation method of the product. The problems that traditional western medicines are large in curative effect fluctuation, have addiction risks, are obvious in side effects and the like are solved, the anti-anxiety characteristic of nicotine and the analgesic and sedative effects of theobromine are used for synergistically exerting a multi-dimensional intervention effect, and curative effect superposition is achieved. The invention also determines the ratio of nicotine to theobromine (the mass ratio of nicotine to theobromine is 1: 100-1000), provides a reliable basis for clinical application, and is beneficial to improving the treatment effect. According to the scheme, the technical blank that natural components are combined to be used for treating bipolar affective disorder is filled, a brand new path is provided for intervention of the disease, and important theoretical exploration value and practical application significance are achieved.

The present invention relates to relates to a non-alkalized, boiled and roasted cocoa solids comprising below 0.14 wt. % of caffeine and below 1.2 wt. % theobromine, preferably below 0.9 wt. % theobromine. The invention also relates to cocoa beverage liquid and powder with these characteristics. Furthermore, the invention relates to a method of making non-alkalized, boiled and roasted cocoa solids, the method comprising: providing cocoa nibs, boiling the cocoa nibs under pressure at a pressure of 1.5-4 bar for a period of 10-60 min, filtering the boiled cocoa nibs to a moisture content of 30-80 wt. %, preferably 50-75 wt. %, roasting the boiled nibs to a moisture content below 4 wt. % and grinding the roasted nibs to provide cocoa liquor, pressing to obtain a cocoa cake, preferably having a fat level 10-12 wt. % or 20-22 wt. % fat, and grinding the cocoa cake to cocoa solid.

The present formulation provides a toothpaste or dentifrice that contains Nano-Hydroxyapatite, Micro-Hydroxyapatite, and Theobromine (with or without Xylitol) to provide a multifaceted approach to prevent caries development, promote caries remineralization (improving the mechanical strength of teeth), and treat tooth hypersensitivity.

A fermentation process for the preparation of xanthine and xanthine derivatives. The disclosure generally relates to a fermentation process for the preparation of xanthine or xanthine derivatives such as 7-methylxanthine (7-MX), theobromine, theophylline, 3-methylxanthine or mixtures thereof. The process results in improved yield and increased efficiency on industrial scale compared to batch process of preparation.

A method for preparing tea lozenges by mixed - strain fermentation of summer and autumn tea and a compound fermenting agent used therein, including Aspergillus cristatus FT128 and Monascus purpureus HQ128. Aspergillus cristatus FT128 was deposited at the China Center for Type Culture Collection on November 9, 2023, with the deposit number CCTCC NO: M20232176; Monascus purpureus HQ128 has been deposited at the China Center for Type Culture Collection, and the deposit date is January 29, 2024, and the strain deposit number is CCTCC NO: M2024269. In the present invention, Aspergillus cristatus FT128 and Monascus purpureus HQ128 are respectively beneficial dominant fungal microorganisms in Fuzhuan tea and dominant fungal microorganisms in red yeast rice. By inoculating them into the extract of summer and autumn tea for mixed - strain liquid fermentation and concentration to prepare the fermented summer and autumn tea extract, and then preparing summer and autumn tea lozenges, the contents of soluble sugar, theobromine, total volatile compounds, monacolin k, and γ - aminobutyric acid are all significantly higher than those of ordinary green tea lozenges; the results show that the mixed - strain fermented summer and autumn tea lozenges have a richer flavor, a more mellow taste, and a higher overall quality.

An aerosol generating material comprising at least two non-tobacco botanical materials, the aerosol generating material produces an aerosol when heated, the aerosol comprising: a first compound selected from the list consisting of: 2-methoxy- phenol, 2-methoxy-4-vinylphenol, vanillin, benzeneacetic acid, phenylethyl alcohol, maltol, and combinations thereof; and a second compound selected from the list consisting of: D-limonene, alpha-terpineol, levomenthol, menthol, 3-methyl butanoic acid, 2-methoxy phenol, terpinene-4-ol, 6-methyl-5-hepten-2-one, 1H-pyrrole-2- carboxaldehyde, alpha-methyl-benzenemethanol, theobromine, octadecanoic acid, n- hexadecanoic acid, methyl-ester-hexadecanoic acid, butyrolactone, caffeine, 9- octadecenoic acid, furfural, 2-methyl butanoic acid, 2,3-dihydrobenzofuran, 2- heptadecanone, and combinations thereof.

The application discloses a theobromine-containing composition and a preparation method and application thereof. The application provides the theobromine-containing composition which comprises the following raw materials in mass fractions: 10-50 parts of microcrystalline cellulose, 0.5-5 parts of inorganic salt, 5-30 parts of theobromine, 1-20 parts of vitamin, 0.2-10 parts of sweetening agent, 1-10 parts of inorganic base, 2-20 parts of sugar alcohol, 2-20 parts of essence, 2-20 parts of humectant and 0.1-2 parts of cooling agent, with the total weight of all the raw materials being 100 parts. The theobromine-containing composition has good taste, rapid absorption, long-lasting effect, convenient carrying and good bioavailability. The theobromine-containing composition has a good application prospect in refreshing and blood pressure reducing products.

It is provided a composition comprising phytic acid or a pharmaceutically acceptable salt thereof, for use in reducing or preventing microorganisms' proliferation and / or encrustations caused by a urinary tract device implanted in a subject, thereby preventing or treating clinical complications related to microorganisms' proliferation and / or encrustations. The composition can further comprise a urine acidifier such as methionine, a crystallization inhibitor such as theobromine and / or other agents.

The present invention relates to a composition comprising at least one psychedelic non-phosphorylated tryptamine, preferably derived from psychedelic psilocybin mushrooms, more preferably derived from psychedelic psilocybin mushrooms belonging to the Psilocybe genus, at least one antioxidant, wherein the antioxidant is at least one carotenoid, and at least one supplement selected from an amino acid, a vitamin, theobromine, caffeine, resveratrol, gamma aminobutyric acid and combinations thereof. The invention relates also to the pharmaceutical uses of the composition of the invention.

The invention discloses a 1-(5-oxohexyl) theobromine sustained-release tablet, the 1-(5-oxohexyl) theobromine sustained-release tablet is composed of a tablet core and a sustained-release coating layer, the tablet core (based on the total weight of the tablet core) is prepared from the following components in parts by weight: 360-450 parts of 1-(5-oxohexyl) theobromine, 50-200 parts of a skeleton sustained-release material, 5-15 parts of an adhesive and 5-10 parts of a lubricant, the matrix sustained-release material is selected from glyceryl behenate and / or polyoxyethylene, preferably, the matrix sustained-release material is selected from glyceryl behenate and polyoxyethylene, the weight ratio of glyceryl behenate to polyoxyethylene is (0.5-2): 1, and the adhesive is prepared into a 95% ethanol solution of 5% povidone K30.

To provide a method capable of determining the selection of a BCG intravesical injection therapy and total cystectomy with less burden on patients.SOLUTION: 2-ethylhydracrylic acid-2, arabinonic acid-2, carnitines, lauric acids, myristic acids, octanoylcarnitines, paraxanthine, S-methylcysteine, S-methylcysteine-S-oxide, SDMA, theobromine, indole-3-acetic acids, pentanoic acids, 1-methyl-4-imidazoleacetic acids, ADMA, alanine, argininosuccinic acids, choline, citrulline, creatinine, guanidoacetic acids, lysine, methionine sulfoxide, myristic acids, N - (1-deoxy-1-fructosyl) leucine, N-acetylneuraminic acids, N6, N6, N6 trimethyllysine, propionylcarnitines, threonine, gamma-glutamyl-glycine, gamma-glutamyl-tyrosine, the method comprises measuring gamma-glutamyl-valine.SELECTED DRAWING: Figure 1

The application discloses a method for rapidly detecting 30 compounds in tea, such as catechins, flavonoids, anthocyanins, phenolic acids and amino acids, wherein the 30 compounds are gallic acid, theobromine, GC, protocatechuic acid, theophylline, EGC, theanine, chlorogenic acid, caffeine, catechin, syringic acid, caffeic acid, vanillic acid, epicatechin, EGCG, delphinidin, salicylic acid, GCG, p-coumaric acid, cyanidin, ferulic acid, sinapic acid, rutin, ECG, pelargonidin, myricetin, theaflavin, quercetin, apigenin and kaempferol. The application can realize simultaneous rapid qualitative and quantitative detection of the 30 compounds, and provides technical support for development of active substances in tea.

The present disclosure provides engineered microbial cells, compositions, and methods of use thereof for producing methylxanthines including theobromine and caffeine.

The invention provides application of a tea tree CsMADS gene in regulating and controlling the content of theobromine in tea leaves. The invention also provides an antisense inhibition treatment liquid for the tea tree CsMADS gene and a kit thereof. The invention further provides a method for increasing the content of theobromine in tea tree buds and leaves. By means of the antisense inhibition CsMADS gene, the content of theobromine in the tea leaves is remarkably increased, and a new way is opened up for accurately regulating and controlling the content of theobromine in the tea leaves and further improving the quality, flavor, nutritional value and the like of the tea leaves.

The application belongs to the technical field of biology, and particularly relates to a method for regulating two alkaloids, theobromine and theacrine, of tea trees by affecting the expression of a CsAP2.7 gene of the tea trees. The application provides the use of the CsAP2.7 gene in regulating alkaloid accumulation in plants, and the nucleotide sequence of the CsAP2.7 gene is shown as SEQ ID NO:1. The application also provides a method for regulating alkaloid accumulation in tea trees, i.e., regulating alkaloid accumulation in plants by inhibiting the expression of the CsAP2.7 gene. The application first constructs an antisense inhibition gene silencing plant of the CsAP2.7 gene of the tea trees and performs a functional study, and finds that the CsAP2.7 gene of the tea trees plays a negative regulation role in the accumulation of theobromine and a positive regulation role in the accumulation of theacrine.

A method for making a sulfur cathode, comprising steps oF mixing sulfur and an amount of a purine compound in a weight ratio of sulfur to purine of from less than 10:1 to greater than 2:1 to form a powder mixture; mixing the powder mixture with a solvent to form a slurry; and drying the slurry onto a current collector to form the sulfur cathode. A cathode for use in a lithium sulfur battery including sulfur; and an purine selected from the group consisting of caffeine, nicotine, morphine, codeine, quinine, atropine, ephedrine, strychnine, colchicine, theobromine, theophylline, papaverine, reserpine, coniine, harmine, ibogaine, vinblastine, vincristine, yohimbine, capsaicin, camptothecin, mitragynine, apomorphine, psilocybin, scopolamine, tetrahydrocannabinol (THC), salvinorin A, mescaline, muscarine, preferably, the purine is caffeine. Also disclosed are electrolytes and batteries.

The invention relates to an oral product for delivery of at least one active ingredient comprising or consisting of —a pouch defining a cavity and —a composition comprising the at least one active ingredient and a carrier selected from the group consisting of, cocoa bean, in particular cocoa bean nibs or cocoa bean powder, coffee bean, cellulose, plant fibres, polysaccharides, oligosaccharides, polyols, and mixtures of at least two of the afore-said carriers, wherein the composition is within the cavity of the pouch and the at least one active ingredient is theobromine. The invention further relates to a method for producing an oral product.

The invention provides a cocoa extract containing homotheobromine, a preparation method and application of the cocoa extract, a tobacco flavor, a cigarette paper coating liquid and a cigarette, and belongs to the technical field of tobacco flavors and fragrances. The preparation method of the cocoa extract with high theobromine content comprises the following steps: extracting or dissolving a cocoa raw material containing theobromine with water, removing insoluble substances, and collecting clear liquid; carrying out membrane separation on the clear liquid, and collecting permeate; adsorbing the permeate through macroporous adsorption resin, removing impurities, eluting, and collecting the eluent to prepare the cocoa extract containing homotheobromine; the macroporous adsorption resin is prepared from one or more of AB-8, X-5, HPD-100 and HJ-04. The theobromine in the cocoa extract is enriched by adopting membrane separation and macroporous adsorption resin, and meanwhile, other natural flavor substances are reserved. The cocoa extract with high theobromine content is singly used or compounded to be used in tobacco flavors, and the comprehensive sensory effect of cigarettes can be remarkably improved.

The present disclosure provides engineered microbial cells, compositions, and methods of use thereof for producing methylxanthines including theobromine and caffeine.

A paraxanthine coffee and its preparation method. The coffee comprises: 0.1%-10% (w / w) paraxanthine, less than 1% (w / w) theobromine and / or theophylline. The preparation method includes steeping coffee beans, weighing paraxanthine to prepare a suspension or solution, roasting coffee beans, and spraying a coating of paraxanthine followed by drying. The coffee is also described as having stimulating, performance-enhancing, and weight-loss effects.