90 results about "Phaeodactylum tricornutum" patented technology

The invention discloses a high-yield mixing culturing method of phaeodactylum tricornutum bohlin and nitzschia closterium, aiming at solving the problems that the phaeodactylum tricornutum bohlin and the nitzschia closterium are individually cultured with an f/2 culture medium in an open cement pit, which causes low growth rate, low yield, easy polluting, and complex operation in the traditional culture method. The culture medium, disclosed by the invention, is characterized in that a mineral water barrel is utilized for mixing culturing of the phaeodactylum tricornutum bohlin and the nitzschia closterium, thus high fatty acid content can be obtained, the cost is low, the pollution is difficult to cause, the operation is easy, and the workload is greatly decreased; in addition, peptone, agar, maltose, potassium sorbate, glucose, sea mud extracting liquor, soil extract liquor, human urine, and cow and sheep manure leaching liquor are added to the culture medium, so that the nutrition is comprehensive and balanced, the growth speed of algae can be greatly raised, and the output is increased by 180%; moreover, the mixing culturing enables the mutualism of the two kinds of algae, and the two kinds of algae can absorb advantages mutually so as to promote each other; nutritive salt is fully utilized, so that the economic benefit is also increased.

The invention relates to a high-density culture process of autotrophic oil-producing microalgae. A fermentation method combining a partition method and a two-step method is adopted, CO2 waste gas discharged from a thermal power plant is used as a carbon source, municipal sewage after living contaminant or miscellaneous algae filtering removal is used as a culture medium, the autotrophic oil-producing microalgae is cultured in a high-density way for growth, perfectly, sea salt is added into the municipal sewage for simulating sea water so that the permeation pressure is increased, the living contaminant or miscellaneous algae pollution is prevented, and the autotrophic oil-producing microalgae is selected from one kind or several kinds of materials from autotrophic chlorella, nannochloropsis salina, botryococcus braunii, chaetoceros gracile, marine green algae, isochrysis galbana, nitzschia closterium, phaeodactylum tricornutum, dunaliella and prism-shaped algae. When the high-density culture process is adopted, high removal efficiency of nitrogen, phosphorus and CO2 can be realized, in addition, the algae concentration, the grease content and the protein content are high, the double effects of environment protection and biological resources production are reached, the operation is simple, convenient and fast, the cost is low, no pollution is caused, social and economic integrated benefits are high, and the high-density culture process is suitable for large-scale popularization and application.

The present invention discloses a method for increasing the fucoxanthin content of Phaeodactylum tricornutum by using methyl jasmonic acid. The method comprises: mixing sterilized seawater and an MAD mother liquor to obtain a nutrition liquid, adding a Phaeodactylum tricornutum mother liquor to the nutrition liquid to culture, culturing for 2 days, adding methyl jasmonic acid, continuously culturing for 6 days, collecting the algae liquid, carrying out centrifugal separation, removing the supernatant, collecting the algae, carrying out a freeze-drying treatment on the algae for 2 days, and grinding for spare. According to the present invention, with the method, the fucoxanthin content in the Phaeodactylum tricornutum can be significantly increased, the operations are simple and convenient, the cost is low, the production cycle is short, and the method is suitable for batch production and industrial production.

The invention discloses a microalgae constitutive expression promoter and application thereof .The promoter PPt49202 is cloned out of phaeodactylum tricornutum bohlin, and the nucleotide sequence is shown as SEQ ID NO.1 .The promoter PPt49202 can promote efficient expression, in the growth cycle of algae cells, of downstream genes, and therefore the microalgae constitutive expression promoter can be applied to genetic engineering and has good application prospects in genetic engineering .

The invention discloses a recombinant vector over-expressing a glucose-6-phosphate dehydrogenase gene, and its application. The recombinant vector comprises a screening labeled gene expression cassette and a target gene expression cassette; the screening labeled gene expression cassette comprises a resistance screening gene, the upstream of the resistance screening gene is fused with an Escherichia coli P1 promoter, and the downstream of the resistance screening gene is fused with an Escherichia coli ccdB terminator; and the target gene expression cassette comprises a Phaeodactylum tricornutum fcpC gene promoter, the glucose-6-phosphate dehydrogenase gene, a Phaeodactylum tricornutum fcpA gene terminator and an Omega leader sequence, and the Omega leader sequence is in the downstream of the Phaeodactylum tricornutum fcpC gene promoter. The recombinant vector is small, is suitable for converting, is easy to operate, is a homologous sequence of the Phaeodactylum tricornutum, and benefits for expression. The over-expression of the glucose-6-phosphate dehydrogenase gene can substantially improve the content of lipids in micro-algal cells, and lays the foundation for the researches of biological energy.

This invention discloses the Phaeodactylum tricornutum trained method and special-purpose Culture medium openly. This invention offers Culture medium is named ZBPT, its prescription is: Sodium nitrate, 135-165mg; potassium dihydrogen phosphate, 32-38mg; Sodium silicate , 215-255mg; Sodium bicarbonate , 400-500mg; KI, 400-1000mg; The trace element solution of f/2, 1ml; Improved vitamin solution of f/2, 1ml; The sea water holds to 1000ml definitely. Including the following step: 1)Dispose the culture medium with the sea water; 2)Phaeodactylum tricornutum density is 3*l05 cell / milliliter, according to make trigonometry brown to mean for1:5-9's proportions algae inoculate reach the culture compared with volume of culture, in 19-25 DEG C, illumination intensity 3000-6000 Lux, illumination time is 8-12 h/day terms keeps in touch with the medium.

The invention discloses a rosa damascena-containing anti-allergy repairing composition and application thereof, and aims at providing the composition capable of effectively removing couperose skin, inhibiting inflammation and improving fine scales. The technical key point is that the composition mainly comprises the following components in parts by weight: 3-8 parts of avena sativa kernel extract,0.1-2 parts of rosa damascena flower oil, 2-8 parts of acer palmatum leaf extract, 1-5 parts of phaeodactylum tricornutum bohlin extract and 1-5 parts of turmeric root extract, and the invention belongs to the technical field of cosmetics.

Disclosed herein are proteins, methods, cells, engineered microorganisms, and kits for generating a modified nucleoside triphosphate transporter from Phaeodactylum tricornutum. Also disclosed herein proteins, methods, cells, engineered microorganisms, and kits for production of a nucleic acid molecule that comprises an unnatural nucleotide utilizing a modified nucleoside triphosphate transporter from Phaeodactylum tricornutum.

The invention discloses a method for improving the content of fucoxanthin in phaeodactylum tricornutum by using purple light. The method comprises the following steps: (1) carrying out inoculating culture: namely inoculating phaeodactylum tricornutum into a culture solution, and carrying out amplification culture in an illumination incubator; (2) carrying out growth measurement: namely measuring the absorbance of the phaeodactylum tricornutum solution every day; and (3) carrying out purple light irradiation: namely carrying out cultivation on the phaeodactylum tricornutum by purple light irradiation when phaeodactylum tricornutum cells grow to a logarithmic phase. According to the method disclosed by the invention, cultivation is carried out on the phaeodactylum tricornutum in the logarithmic phase by purple light irradiation, so that the content of fucoxanthin in the phaeodactylum tricornutum is greatly improved. In addition, the method has simple steps and is easy to operate, the used instrument is relatively normal, and the method is convenient and environmentally friendly.

The invention discloses a phaeodactylum tricornutum culture medium. The phaeodactylum tricornutum culture medium comprises, by mass concentration, 30-50 mg/L of NH4NO3, 5-8 mg/L of NaH2PO4, 2-5 mg/L of FeCl3, 3-6 mg/L of Na2EDTA, 0.005-0.01 mg/L of VB1, 0.01-0.05 mg/L of VB12, 22 to 24 microgram/L of ZnSO4.4H2O, 175-180 microgram/L of MnCl2.4H2O, 9-11 microgram/L of CuSO4.5H2O, 7.0-7.5 microgram/L of Na2MoO4.2H2O and 11-13 microgram/L of CoC12.6H2O. The phaeodactylum tricornutum culture medium further comprises 0.1-1 mg/L of a plant growth regulatory factor, namely, triacontanol, and 0.1-1 mg/L of scandium salt. The culture medium has the advantages of being rapid in growth rate and high in cell density when used for culturing phaeodactylum tricornutum, and is suitable for large-scale batched production.

The present invention discloses a method for increasing the fucoxanthin content of Phaeodactylum tricornutum by using ammonium ceric sulfate. The method comprises: mixing sterilized seawater and an MAD mother liquor to obtain a nutrition liquid, adding a Phaeodactylum tricornutum mother liquor to the nutrition liquid to culture, culturing for 2 days, adding ammonium ceric sulfate, continuously culturing for 6 days, collecting the algae liquid, carrying out centrifugal separation, removing the supernatant, collecting the algae, carrying out a freeze-drying treatment on the algae for 2 days, grinding for spare, dissolving the grinded algae with dehydrated alcohol, standing for 1 h at a temperature of 4 DEG C, and centrifugating for 10 min at a temperature of 4 DEG C at a speed of 13000 rpm to obtain the supernatant. According to the present invention, the operations are simple and convenient, the cost is low, the production cycle is short, and the method is suitable for batch production and industrial production.

The invention provides a detecting method for construction of a genetic transformation system of phaeodactylum tricomutum. The detecting method is characterized by comprising the following steps of: (1) construction of GFP gene recombinant plasmid, and extraction of the recombinant plasmid for carrying out transformation on the phaeodactylum tricomutum; (2) transformation by a gene gun; and (3) observation of a fluorescence microscope. The detecting method has the advantages of being simple, fast, easy to implement, economic and applicable.

The invention provides a method for producing fucoxanthin by using a mutant strain for expressing exogenous pyruvate phosphate dikinase in phaeodactylum tricornutum, and particularly relates to algae preference codon optimization on an original CyPPDK gene to obtain a new CyPPDK gene with a nucleotide sequence as shown in SEQ ID NO.7; the constructed shuttle expression vector is named as pPha-fcp-PPDK; and the obtained new gene is introduced into a host algae cell phaeodactylum tricornutum cell in an electric transformation manner, and a transformant is screened to obtain a phaeodactylum tricornutum transgenic algae strain for overexpression of pyruvate phosphate dikinase. Compared with an unoptimized transformant, the optimized transformant for phosphorylation sites of the CyPPDK gene has the advantages that the biomass accumulation is improved by 33.3%, the content of EPA in grease is improved by 11.5%, and the fucoxanthin yield is improved by 21.2%.

The invention provides an aureococcus anophagefferens algistat and a preparation method for the same. The preparation method for the aureococcus anophagefferens algistat comprises the following steps: (1) cultivating phaeodactylum tricornutum to a later exponential growth stage to obtain an alga solution; (2) performing vacuum suction filtration on the alga solution to obtain alga cell-free filtrate; (3) performing extraction, reduced pressure concentration and nitrogen concentration on the alga cell-free filtrate to obtain the aureococcus anophagefferens algistat. The aureococcus anophagefferens algistat is derived from natural microalgae, is high in environmental affinity, can quickly act for algal inhibition, and has long-lasting effects; in addition, the preparation method is simple and low in preparation cost, and can be widely applied to inhibition and killing of aureococcus anophagefferens in offshore areas, aquaculture ponds and the like on a large scale.

The invention discloses a method for preparing an enteromorpha extract solution for inhibiting prorocentrum micans and phaeodactylum tricornutum bohlin, belongs to the technical field of medicines, in particular relates to a method for preparing an atropa belladonna extract and provides a method for preparing the atropa belladonna extract, wherein the method is short in process flow, high in product purity, sewage-free, environment-friendly and pollution-free. The preparation method comprises the following steps: by taking herba belladonnae as a raw material, crushing the raw material, adding a sulfuric acid or hydrochloric acid solution for stirring, filtering the stirred solution, adsorbing and separating the filtered solution by using resin, eluting by using an ethanol solution, and concentrating the eluate to obtain the atropa belladonna extract. Because a normal-temperature acid solution extraction method is adopted, the dissolution of chlorophyll and impurities is small, and the atropa belladonna alkaloid is easily extracted from the acid solution, so that the active ingredient content in the extracted solution is high, and fewer impurities are contained in the extracted solution.

The invention discloses a phaeodactylum tricornutum culture medium. A formula of the culture medium comprises tryptone. Preferably, the formula contains a mixed nitrogen source. The culture medium isfavorable for accelerating the growth speed of the phaeodactylum tricornutum, increasing the cell density and promoting the fucoxanthin accumulation so as to improve the culture efficiency. The phaeodactylum tricornutum culture medium is particularly suitable for large-scale culture.

The invention provides a method for preparing biodiesel from marine microalgae, comprising the steps of: cleaning Phaeodactylum tricornutum, carrying out plate-and-frame filter pressing to remove water, adding the wet Phaeodactylum tricornutum into water, adding cellulase, protease and sodium citrate, mixing the materials, carrying out enzymolysis at 45-55 DEG C for 2-5h, carrying out enzyme deactivation, regulating the pH value of the obtained enzymolysis liquid to 9.0-10.0, adding an equal volume of chloroform-methanol, carrying out extraction, adding the organic phase to mixed liquid of concentrated sulfuric acid, tetrahydrofuran and methanol, standing after shaker oscillation, washing and centrifuging the obtained ester phase with petroleum ether-95v/v%ethanol to obtain an oil phase and concentrating the oil phase. The preparation method has the advantages of high yield of biodiesel, good quality, simple production process and low cost and can be widely popularized.

The invention relates to the field of genetic engineering and metabolic engineering and in particular to a phaeodactylum tricornutum bifunctional enzyme (wax ester synthetase/diacylglycerol glycerinum acyltransferase) and application thereof. The phaeodactylum tricornutum bifunctional enzyme is wax ester synthetase/diacylglycerol glycerinum acyltransferase, the amino acid residue of the enzyme is as shown in SEQ ID No:1 in a sequence table, or has 90% or greater of homology of an amino acid as shown in SEQ ID No:1 in the sequence table, and is capable of encoding sequences of proteins of same functions. The phaeodactylum tricornutum bifunctional enzyme, namely the wax ester synthetase/diacylglycerol glycerinum acyltransferase can be applied to metabolic engineering research of microalgae, is capable of increasing the content of lipid, and has wide application prospects in development of biodiesel. Genes of the wax ester synthetase/diacylglycerol glycerinum acyltransferase are over-expressed in phaeodactylum tricornutum, mutant strains of which the grease content is remarkably increased are acquired, and the enzyme can be applied to biodiesel production processes.

The invention relates to a method used for rapid nondestructive detection of fucoxanthin in Phaeodactylum tricornutum cells. The method is capable of realizing rapid nondestructive detection of fucoxanthin in Phaeodactylum tricornutum cells, and simplifying detection steps greatly; after establishment of a standard curve, extraction operation of samples is not needed in sample detection, sample pretreatment is simple, detection time is shortened, and at the same time, the method is especially suitable to be used for rapid nondestructive detection of fucoxanthin in microalgae cells, especiallyin Phaeodactylum tricornutum cells.

The invention discloses a method for efficiently collecting phaeodactylum tricornutum. The method comprises the following steps: (1) adding calcium chloride and dipotassium phosphate; (2) forming calcium phosphate precipitate; and (3) filtering with a filter press. The method is simple and quick to operate, and does not influence fucoxanthin in phaeodactylum tricornutum cells.

The invention discloses compound feed for preventing tilapia streptococcosis. The compound comprises raw materials in parts by weight as follows: 20-40 parts of fermented soybean meal, 10-16 parts ofdark plums, 10-14 parts of phaeodactylum tricornutum powder, 10-16 parts of earthworm powder, 8-13 parts of tea tree oil, 10-14 parts of fiveleaf gynostemma herb, 8-14 parts of lysimachia sikokiana, 14-18 parts of licorice juice, 10-14 parts of rhodiola rosea, 4-8 parts of a radix notogrinseng extract, 3-7 parts of rhizoma atractylodis macrocephalae and 12-16 parts of green juice powder. The compound feed has the benefits of promoting growth of tilapia and enhancing resistance of the tilapia.

The invention relates to the field of gene engineering, and especially relates to a promoter and an application thereof. The promoter of gene Pt48882 is cloned from Phaeodactylum tricornutum, and the nucleotide sequence of the promoter is represented by SEQ ID NO.1. The promoter PPt48882 can promote high-level expression of downstream gene in the growth period of microalga cells, so the promoter can be applied to the gene engineering, and has good application prospect in the gene engineering.