Method for increasing fucoxanthin content of Phaeodactylum tricornutum by using ammonium ceric sulfate

A technology of Phaeodactylum fucoxanthin and ammonium cerium sulfate, which is applied in the field of biomedicine to achieve the effect of increasing the content, increasing the content and reducing the cost

Inactive Publication Date: 2015-04-22
NINGBO UNIV
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  • Description
  • Claims
  • Application Information

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  • Method for increasing fucoxanthin content of Phaeodactylum tricornutum by using ammonium ceric sulfate
  • Method for increasing fucoxanthin content of Phaeodactylum tricornutum by using ammonium ceric sulfate
  • Method for increasing fucoxanthin content of Phaeodactylum tricornutum by using ammonium ceric sulfate

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Embodiment 1

[0036] Embodiment 1 The influence of ammonium cerium sulfate on fucoxanthin in Phaeodactylum tricornutum

[0037] Take 18 groups of 300ml sterilized MAD culture solution, respectively, and insert them into Phaeodactylum tricornutum algae at a ratio of 1:10. Use 0.2mg / L, 0.4mg / L, 0.8mg / L, 1.6mg / L, 2mg / L ammonium cerium sulfate to treat Phaeodactylum tricornutum and 3 parallel groups without any treatment. From the day when Phaeodactylum tricornutum was inserted into the culture medium, the concentration of each group of algae liquid was measured by spectrophotometer every day, and then measured for another 6 days.

[0038] The result is as figure 1 As shown, when different concentrations of cerium ammonium sulfate (CAS) were added, the absorbance value of each group did not change significantly. The OD value of algae treated with L ammonium cerium sulfate was the smallest. Wherein the OD value of the control group was 0.4322, and the OD value of the 0.4mg / L experimental grou...

Embodiment 2

[0040] Take 400ml of sterilized seawater for 7 groups, and add 400μl of MAD mother solution respectively. Add 40ml of Phaeodactylum tricornutum mother solution to the culture solution and cultivate in a light incubator. After 2 days of cultivation, different concentrations of ammonium cerium sulfate were added to the 6 groups of algae culture solutions, the concentrations were 0mg / L, 0.2mg / L, 0.4mg / L, 0.8mg / L, 1.6mg / L, 2.0mg / L, respectively. mg / L.

[0041] Such as figure 2 As shown, from the determination of the growth curve of Phaeodactylum tricornutum, it can be known that Phaeodactylum tricornutum has reached the end of the logarithmic phase after culturing to the 6th day, and the maximum biomass can be obtained. Therefore, it is necessary to collect the algae liquid when cultivating to the 6th day.

[0042]Take 80ml of each group of Phaeodactylum tricornutum into a centrifuge tube, centrifuge at 4°C, 5000rpm for 10min, discard the supernatant, take 1ml of MAD seawater ...

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Abstract

The present invention discloses a method for increasing the fucoxanthin content of Phaeodactylum tricornutum by using ammonium ceric sulfate. The method comprises: mixing sterilized seawater and an MAD mother liquor to obtain a nutrition liquid, adding a Phaeodactylum tricornutum mother liquor to the nutrition liquid to culture, culturing for 2 days, adding ammonium ceric sulfate, continuously culturing for 6 days, collecting the algae liquid, carrying out centrifugal separation, removing the supernatant, collecting the algae, carrying out a freeze-drying treatment on the algae for 2 days, grinding for spare, dissolving the grinded algae with dehydrated alcohol, standing for 1 h at a temperature of 4 DEG C, and centrifugating for 10 min at a temperature of 4 DEG C at a speed of 13000 rpm to obtain the supernatant. According to the present invention, the operations are simple and convenient, the cost is low, the production cycle is short, and the method is suitable for batch production and industrial production.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a method for increasing the content of fucoxanthin in Phaeodactylum tricornutum by using ammonium cerium sulfate. Background technique [0002] Fucoxanthin is a class of orange-yellow carotenoids mainly found in edible brown algae. Its chemical structure is a special polyene chain (conjugated double bond, epoxy group and conjugated carbonyl group with oxidation resistance). It is because of its structure that fucoxanthin is also considered as a class of lutein. Fucoxanthin plays an extremely important role in the treatment of human diseases. In the anti-obesity, anti-cancer and other diseases, the research on its mechanism of action also has a certain foundation. [0003] Phaeodactylum tricornutum has been paid attention and researched because of its ability to synthesize high content of oil. Studies have found that fucoxanthin exists in Phaeodactylum tricornut...

Claims

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Application Information

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IPC IPC(8): C12N1/38C12N1/12C12R1/89
CPCC12N1/12C12N1/38
Inventor 朱帅旗龚一富刘浩刘诗洁魏朝广刘钊王何瑜
Owner NINGBO UNIV
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