The invention discloses an Internet & hierarchical diagnosis and treatment service system, comprising a cloud server, lower-level hospital servers, upper-level hospital servers, a total database, medical personnel in upper-level and lower-level hospitals and patient users, wherein the cloud server is provided with a qualification interconnection auditing module, a patient entrance module, a hospital guiding and triage module and a medical service supervision function module. The medical service supervision function module can make full use of medical resources of hospitals at all levels through the hierarchical diagnosis and treatment service system so as to improve the doctor-seeing experience of patients. Medical staff in the upper-level and lower-level hospitals cooperate with each other, so two-way referral and remote diagnosis and treatment are realized, reservation services including initial visiting of patients, subsequent visiting of the patients, rehabilitation, nursing, report interpretation, medicine distribution, chronic disease follow-up visit management, outpatient service, hospitalization, examination, operation and the like are provided, the treatment requirements of the patients are fully met, rational flow of the patients is guided, and meanwhile the patients can equally enjoy high-quality medical service of third-level first-class hospitals.