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Detection method of traditional Chinese medicinal composition for treating waist and knee pains and sciatica

A technology for sciatica and detection methods, applied in the field of analytical chemistry, can solve problems such as unclear feature points and poor map separation

Active Publication Date: 2017-07-04
GUANGZHOU CHEN LI JI PHARMA FACTORY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The TLC characteristic map of dog ridge is not well separated, and the feature points are not clear
The current standard of this variety has no content determination of index components

Method used

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  • Detection method of traditional Chinese medicinal composition for treating waist and knee pains and sciatica
  • Detection method of traditional Chinese medicinal composition for treating waist and knee pains and sciatica
  • Detection method of traditional Chinese medicinal composition for treating waist and knee pains and sciatica

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0086] Example 1 Detection of dog ridge by thin layer chromatography

[0087] 1) Preparation of the test solution: take 3.5g of the product with batch numbers AX7721, AX7771 and VO6013, grind finely, add 50ml of hydrochloric acid ethanol solution (1.35→100), ultrasonication for 30 minutes, evaporate the filtrate to dryness, and ultrasonicate with 5ml of methanol To dissolve, filter, as the test solution.

[0088] 2) Preparation of the reference medicinal material solution: take 2 g of the dog spine reference medicinal material, and prepare the reference medicinal material solution according to the same method as step 1.

[0089] 3) Preparation of negative sample solution: take a negative sample without dog ridge, and prepare negative sample solution according to the same steps and methods as the test solution.

[0090] 4) Detection: According to the thin-layer chromatography (Appendix VI B of "Chinese Pharmacopoeia" 2010 edition), draw 4 μl of each of the above-mentioned so...

Embodiment 2

[0097] Example 2 Detection of Black Tiger by Thin Layer Chromatography

[0098] 1) Preparation of the test solution: Take 4g of the product with batch numbers AX7721, AX7771 and VO6013 respectively, grind it finely, put it in a stoppered Erlenmeyer flask, add 10ml of water to moisten it, then add 50ml of ethyl acetate, and ultrasonicate for 30 minutes , Cool, filter, evaporate the filtrate to dryness, add methanol 2ml to dissolve the residue, and use it as the test solution.

[0099] 2) Preparation of the reference medicinal material solution: take 2 g of the black tiger reference medicinal material, and prepare the reference medicinal material solution according to the same method as step 1.

[0100] 3) Preparation of negative sample solution: take a negative sample that does not contain black tiger, and prepare a negative sample solution according to the same steps and methods as the test solution.

[0101] 4) Detection: According to the thin-layer chromatography (Appendi...

Embodiment 3

[0102] Example 3 Thin Layer Chromatography for the Detection of Sangjisi

[0103] 1) Preparation of the test solution: take 10 g of the product with batch numbers AX7721, AX7771 and VO6013 respectively, grind finely, add 5 ml of water to moisten, add 60 ml of ethyl acetate, soak overnight, ultrasonically treat for 30 minutes, filter, The filtrate was evaporated to dryness, and the residue was dissolved by adding 0.5ml of methanol as the test solution.

[0104] 2) Preparation of the reference medicinal material solution: take 1 g of the reference medicinal material Sangjisi, add 2 ml of water to make it moist, add 30 ml of ethyl acetate, and prepare the reference medicinal material solution according to the same method as step 1.

[0105] 3) Preparation of negative sample solution: take a negative sample that does not contain mulberry, and prepare a negative sample solution according to the same steps and methods as the test solution.

[0106] 4) Detection: test according to...

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Abstract

The invention provides a detection method of a traditional Chinese medicinal composition for treating waist and knee pains and sciatica. Raw materials of the traditional Chinese medicinal composition comprise Rhizoma Cibotii, Fructus Rosae Laevigatae, Caulis Spatholobi, Philippine flemingia root, Kadsura coccinea, Millettia specisoa Champ, Ligustri Lucidi Fructus, Chinese Taxillus Twig, semen cuscutae, Rhizoma Corydalis, Zanthoxylum nitidum, Boswellia carteri and myrrh. The detection method comprises the following steps: detecting the content of (2'S)-Kadsura longepedunculata lignin J in the Kadsura coccinea through high performance liquid chromatography; and detecting the content of specnuezhenide in the Ligustri Lucidi Fructus through the high performance liquid chromatography, and detecting the Kadsura coccinea, the Rhizoma Cibotii and the Chinese Taxillus Twig through thin layer chromatography. The invention also concretely discloses conditions and concrete operating steps of all above detection technologies. The detection method allows five raw materials to be qualitatively and quantitatively detected, and the quantity of the detected raw materials is 1 / 3 of the total quantity of the raw materials, so the quality of medicines is effectively guaranteed, and medical demands are met.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and in particular relates to a detection method of a traditional Chinese medicine composition for treating waist and knee pain and sciatica. The traditional Chinese medicine composition is Shujinjianwaist Pill. Background technique [0002] Shujinjianwaist Pills are recorded in the twelfth volume of traditional Chinese medicine preparations in the "Drug Standards of the Ministry of Health of the People's Republic of China", and the standard number is WS 3 -B-2441-97. Shujinjianwaist Pills are made of thirteen medicines such as Gouji, Rosa japonica, Caulis Spatholobus, Qianjinba, Black Tiger, Niu Dali, Ligustrum lucidum, Mulberry, Cuscuta, Corydalis Corydalis, Two-faced Acupuncture, Frankincense, and Myrrh. , has the effect of nourishing liver and kidney, strengthening muscles and bones, expelling wind and dehumidification, activating collaterals and relieving pain, and is used for the treatme...

Claims

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Application Information

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IPC IPC(8): G01N30/88
CPCG01N30/88
Inventor 杨能英何风雷彭富全陈明权
Owner GUANGZHOU CHEN LI JI PHARMA FACTORY
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