3 results about "Quantitative PCR analysis" patented technology

This invention discloses the artificial synthesis of the disease-resistant gene NLP27 and its application, belonging to the field of plant genetic engineering technology. This invention selects a conserved 27-amino acid fragment from Verticillium dahliae NLP1, modifies its nucleotide sequence, and synthesizes the NLP27 gene, which is then overexpressed in potato plants through genetic transformation. Inoculation analysis shows that transgenic plants with the NLP27 gene exhibit high resistance to potato late blight and scab. Quantitative PCR analysis shows that excessively high NLP27 transcript levels significantly inhibit potato plant growth. After screening for expression levels and disease resistance, three transgenic lines with moderate NLP27 expression levels (such as PT1, PT6, and PT7) were obtained. These lines significantly enhanced resistance to potato late blight and scab without sacrificing yield.

This invention discloses a barley stripe disease resistance gene. Rdg2b This invention relates to the field of bioengineering technology and its applications. It describes the cloning of [specific species / organisms] from Ganbei No. 2 barley. Rdg2b The complete CDS segment of the gene was extracted, and an overexpression vector was constructed. Agrobacterium-mediated transformation was then performed to obtain overexpression-positive lines. Infection experiments with *Strombus striatum* showed that, compared to the wild type, [the expression was positive]. Rdg2b Overexpression lines showed significantly enhanced disease resistance. Real-time quantitative PCR analysis revealed that overexpression lines exhibited... Rdg2b Gene expression was significantly upregulated, confirming Rdg2b This invention positively regulates barley disease resistance. It provides important genetic resources for molecular breeding of barley for disease resistance.

The invention discloses reference genes for real-time fluorescent quantitative PCR detection of different tissues of rhizoma bletillae in different growth periods, and belongs to the technical field of plant molecular biology, the reference genes are BsGAPDH and BsEIF, the nucleotide sequence of the BsGAPDH is as shown in SEQ ID NO.1, and the nucleotide sequence of the BsEIF is as shown in SEQ ID NO.2. Three different tissues of rhizoma bletillae in seven growth periods are used as experimental materials, and the reference genes are used for real-time fluorescent quantitative PCR detection of different tissues of rhizoma bletillae in seven growth periods. The reference genes BsGAPDH and BsEIF with stable expression are obtained through fluorescent quantitative PCR (polymerase chain reaction) analysis and gene expression stability analysis by various technologies, and the two genes are stably expressed in different tissues in different growth periods of rhizoma bletillae and can be used as reference genes for researching gene expression in different tissues in different growth periods of rhizoma bletillae. The method can lay a foundation for functional research of genes related to accumulation of traditional Chinese medicinal components of rhizoma bletillae, and has relatively good practical value and scientific research application value.