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Method for screening reference gene in real-time quantitative PCR analysis of heterodera glycines infected wild soybean root tissue

A soybean cyst nematode and wild soybean technology, applied in the fields of biology and genetics, can solve problems such as soybean stability evaluation that have not yet been seen

Inactive Publication Date: 2017-11-10
JILIN ACAD OF AGRI SCI
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Problems solved by technology

However, the studies on the expression of genes related to soybean cyst nematodes all use Actin or Actin11 as the internal reference gene, and there are no reports on the evaluation of the expression stability of soybean housekeeping genes in cultivated soybean or wild soybean under the infection of soybean cyst nematode.

Method used

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  • Method for screening reference gene in real-time quantitative PCR analysis of heterodera glycines infected wild soybean root tissue
  • Method for screening reference gene in real-time quantitative PCR analysis of heterodera glycines infected wild soybean root tissue
  • Method for screening reference gene in real-time quantitative PCR analysis of heterodera glycines infected wild soybean root tissue

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Embodiment Construction

[0025] In order to make the technical means, creative features, achievement goals and effects realized by the present invention easy to understand, the present invention will be further described below in conjunction with specific embodiments, but the following embodiments are only preferred embodiments of the present invention, not all. Based on the examples in the implementation manner, other examples obtained by those skilled in the art without creative work shall fall within the protection scope of the present invention. The experimental methods in the following examples are conventional methods unless otherwise specified, and the materials, reagents, etc. used in the following examples can be obtained from commercial sources unless otherwise specified.

[0026] The screening method for the internal reference gene in the real-time quantitative PCR analysis of wild soybean root tissue under soybean cyst nematode infection is characterized in that: the test materials are resp...

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Abstract

The invention provides a method for screening a reference gene in the real-time quantitative PCR analysis of a heterodera glycines infected wild soybean root tissue, and relates to the fields of biology and genetics. A high-resistance high-sensitive wild soybean germplasm is taken as a test material, the condition of gene expression of 24 candidate housekeeping genes in a wild soybean root system in different heterodera glycines infection periods is detected, the expression abundance and the expression stability are evaluated so as to screen the reference gene suitable for studying the expression of the gene in the heterodera glycines infected wild soybean root system, and the method is used for exploring the resistance mechanism of the wild soybean to the heterodera.

Description

technical field [0001] The invention relates to the fields of biology and genetics, in particular to a screening method for an internal reference gene in the real-time quantitative PCR analysis of wild soybean root tissue infected by soybean cyst nematode. Background technique [0002] Real-time quantitative PCR (qRT-PCR) is a powerful technique for studying gene expression. It has the advantages of high sensitivity, good repeatability, and accurate quantification. It is widely used in crop molecular biology and genetics research. and other fields. The internal reference gene is the key factor for the accuracy of qRT-PCR analysis, and the selection of a suitable internal reference gene is an important prerequisite for qRT-PCR research. Housekeeping genes constitutively expressed in plants are the basic components of the organelle skeleton necessary for plant life-sustaining activities, or participate in basic biochemical metabolic processes, such as actin gene ACT, α / β tubu...

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/686C12Q1/6895C12Q2600/13C12Q2600/166C12Q2561/113C12Q2545/114C12Q2521/107C12Q2545/101
Inventor 袁翠平董英山赵洪锟王玉民刘晓冬齐广勋王英男李玉秋
Owner JILIN ACAD OF AGRI SCI
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