The invention relates to a
microelectrode array as well as a
surface modification method and application thereof, belongs to the technical field of biosensors, and aims at solving one of the problems that a traditional
electrode in the prior art is complicated in preparation and low in sensitivity and cannot detect
glutamic acid and
dopamine at the same time. According to the method disclosed by the invention, the
polypyrrole films are deposited on the
dopamine detection
microelectrode and the
glutamic acid detection
microelectrode, so that interference substances can be isolated. Meanwhile, the interference of
dopamine with positive charges also needs to be eliminated in addition to the
elimination of AA on the
glutamic acid detection site; according to the invention, the selected
Nafion membrane is a cation selective permeable membrane, and cations can pass through the membrane to isolate anions; the
peroxide polypyrrole thin film is also a cation exchange membrane,
polypyrrole is formed through electropolymerization of
pyrrole, then high-potential electrooxidation is carried out, and in the electrooxidation process, electronegative carbon groups formed along the main chain of the
peroxide polypyrrole can effectively attract cations and repel anions.