62results about How to "Sync detection" patented technology

An establishment method of a gene library of fecal flora based on high-throughput gene sequencing employs a ''nested PCR'' method for enrichment amplification on 16S rDNA, so as to reduce the host and food residue genome pollution by the maximum; and V3 and V6 are combined for specific amplification and massive parallel sequencing, so as to obtain the a target gene sequence database of the flora. The library can increase the identification of the bacterial flora from Genus level to Species level. The method can maximally avoid the interference of host cell nucleic acid, and completely and efficiently detect 16SrDNA tag sequence of all bacteria varieties, so as to accurately determine the ecological structure of the intestinal flora.

The invention relates to an immunochromatography test strip for synchronously detecting the mixed pollution of aflatoxin, ochratoxin A and zearalenone, and a preparation method. The immunochromatography test strip comprises a paperboard, wherein a water-absorbing pad, a detection pad, a gold label pad and a sample pad are sequentially bonded on one surface of the paperboard from top to bottom, and the adjacent pads are connected at a junction in an overlapping manner; the detection pad takes a nitrocellulose membrane as a base pad, and a transversal quality control line and detection lines are arranged on the detection pad; three detection lines are located below the quality control line, distributed at intervals, and coated with OTA-BSA (ochratoxin A-bovine serum albumin), ZEA-BSA (zearalenone A-bovine serum albumin) and AFB1-BSA (aflatoxin B1-bovine serum albumin) respectively; and the quality control line is coated with a rabbit anti-mouse polyclonal antibody; the gold label pad is transversally spayed with a nanogold labelled anti-aflatoxin universal monoclonal antibody, a nanogold labelled anti-ochratoxin A monoclonal antibody and a nanogold labelled anti-zearalenone monoclonal antibody. The immunochromatography test strip can be used for synchronous detection for aflatoxin, ochratoxin A and zearalenone, as well as is simple and rapid to operate, and high in sensitivity.

The invention discloses a nucleic acid sensor based on quantum dots and a preparation method and a detection method thereof, wherein the nucleic acid sensor comprises a solid-phase holder and an oligonucleotide probe array fixed on the surface of the solid phase holder, wherein the oligonucleotide probe array comprises an oligonucleotide probe which does not contain self-complementary sequences; one end of the oligonucleotide probe marks quantum dots and is fixed on the surface of the solid-phase holder by the quantum dots; or the oligonucleotide probe array comprises a molecular beacon, wherein one end of the molecular beacon marks quantum dots and is fixed on the surface of the solid-phase holder by the quantum dots, and the other end of the molecular beacon is marked by a fluorescence quenching group. By utilizing the basic-group complementation pairing principle and the FRET phenomenon, the detection of the special nucleic acid sequences in a nucleic acid sample can be simultaneously achieved; in addition, the invention has simple preparation and accurate, sensitive, simple, convenient and rapid detection and can detect a plurality of samples simultaneously.

The invention relates to a time-resolved immunochromatography kit for synchronously detecting aflatoxin and carbaryl mixed pollution, and a preparation method and an application thereof. The kit comprises an immunochromatography time-resolved fluorescence test strip and a sample reaction bottle containing lyophilized products of an europium-labeled anti-aflatoxin monoclonal antibody and an europium-labeled anti-carbaryl monoclonal antibody, wherein the detection line of the immunochromatography time-resolved fluorescence test strip is respectively coated with an aflatoxin-bovine serum albumin conjugate and a carbaryl-ovalbumin conjugate; and the anti-carbaryl monoclonal antibody is secreted by a hybridoma cell strain Jnw1D2 with the preservation number of CCTCC NO.C201654. The kit can be used for synchronously detecting the content of aflatoxin and carbaryl in a sample, and has the characteristics of simplicity in operation, rapidness and high sensitivity.

The invention discloses a quantitative detection method for a gene copy number. The method comprises the following steps of: respectively designing primers and fluorescence probes for target gene and reference gene amplification sequences, and designing a closed probe for the target gene amplification sequence; detecting a sample: performing multiple TaqMan fluorescent quantitative polymerase chain reaction (PCR) on target genes and reference genes by using the DNA sample to be detected as a template, and respectively recording Ct values by using a DNA sample with known target gene copy number as a reference sample; and analyzing the result. By the method, the copy number of the functional genes can be directly and quantitatively detected, and deletion, deletion number and multiple copies (CNVs) of the detected genes are directly reflected, so that synchronous quick detection of gene deletion and multiple gene copies is realized; and the method is high in sensitivity, the synchronous quick detection of the gene deletion and the multiple gene copies can be finished only by a common fluorescent quantitative PCR instrument, and experiments prove that the method is accurate and reliable.

The invention relates to the field of lead screw detection, in particular to a lead screw lead accuracy detection device. The lead screw lead accuracy detection device comprises a mechanical portion, a movement control portion, a data collection portion and a computer. The mechanical portion comprises an instrument main frame, a detection rail, a linear grating ruler, a circular grating pair and a detection head device. The movement control portion comprises a motor, a motor driver and a movement control card. The data collection portion comprises a data collection card and a signal transferring board. Detection software is arranged on the computer, the computer is connected with the movement control card and the data collection card through a data bus, and the computer controls the motor in real time, conducts data collection of grating signals of the circular grating pair and grating signals of the linear grating ruler in real time, and displays a result and an error curve after conducting processing through the algorithm in the detection software. The lead screw lead accuracy detection device is used for detecting the accuracy of the lead of a lead screw.

The invention discloses a kit for synchronously and rapidly detecting various zoonotic pathogens. The kit comprises immunomagnetic beads and a quantum dot fluorescent bioprobe; the immunomagnetic beads are obtained by activating carboxylated magnetic beads by EDC and NHS and coupling the beads with a trivalent yolk antibody IgY; the quantum dot fluorescent bioprobe is obtained by coupling fluorescent quantum dots with different colors with anti-escherichia coli O157: H7, anti-Listeria monocytogene, anti-Brucella and anti-vibrio parahaemolyticus rabbit polyclonal antibodies respectively; the trivalent yolk antibody IgY is obtained by deactivating escherichia coli O157: H7, Listeria monocytogene and brucella, and immunizing an SPF chicken. When the kit provided by the invention is used for performing synchronous rapid detection of four pathogens, the kit has the advantages of good specificity, high sensitivity, good reproducibility, good stability and short detection time; the kit can be applied to simultaneous rapid quantitative detection of the four pathogens.

Belonging to the technical field of nucleic acid diagnosis, the invention particularly relates to a method and a kit for synchronous extraction and synchronous detection of hepatitis and HIV virus by fluorescent complementary probe hybridization. With a magnetic bead as an automation medium, the method includes a technique for specific synchronous capture of HBV, HCV, and HIV-1 nucleic acids through biotin and oligonucleotide modified capture probes and a Taqman probe-based fluorescent complementary probe hybridization technique for real-time synchronous detections. The corresponding kit includes a disinhibition agent, a lysate, a magnetic bead suspension, a washing liquid, an eluent, an internal control, a 2 * Annealing Buffer, fluorescent probes, a positive control, and a negative control. The method and the kit provided in the invention have the characteristics of: no need of PCR reaction, single tube operation, closed detection, high degree of automation, synchronous extraction of various virus nucleic acids, and synchronous real-time detection, high sensitivity, good specificity and high precision, thus being suitable for large-scale blood screening, clinical examination and scientific research units.

The invention relates to an ignition and flame detection integrated device which comprises a mounting base. The device is characterized in that a connecting pipe is arranged on the side wall of the mounting base and is connected with an ejector, the lower ends of the ejector are respectively provided with a fuel gas inlet and a compressed air inlet, an electrode ceramic base is fixed at one end of the mounting base, the other end of the mounting base is provided with a guide pipe, an igniting electrode/ion probe is fixed on the electrode ceramic base, one end of the igniting electrode/ion probe passes through the mounting base and the guide pipe, extends out of the guide pipe and finally reaches an outlet of the guide pipe, the other end of the igniting electrode/ion probe is connected with a secondary side of an ignition transformer through a high-voltage lead, a primary side of the ignition transformer is connected with a power supply, the secondary side is earthed, the secondary side of the ignition transformer, which is at the end opposite to the igniting electrode/ion probe, is connected with a flame amplifier and the flame amplifier is earthed. The ignition and flame detection integrated device is simple, compact and reasonable in structure, convenient to mount, practical and easy to operate, has good burning effect and low possibility of backfire, is safe and reliable and can perform flame formation and detection at the same time.

The invention relates to a non-invasive fetal electrocardiogram detection device and an electrocardiogram signal data processing method. The device comprises an electrocardiogram acquisition front-enddevice and an electrocardiogram signal analysis processing software system. It includes ECG collector, analog-to-digital conversion module, wireless communication module and core processor module. The signal obtained by the ECG acquisition front end is transmitted to the core processing module through the wireless communication module, The ECG signal quality evaluation, pretreatment, maternal ECGsignal analysis and fetal ECG signal analysis are completed, and the heart rate information of mother and fetus is obtained. Combined with waveform morphology analysis, the maternal health status andfetal intrauterine status are evaluated. The device can realize the long-term, dynamic and real-time analysis and processing of fetal signals, which is of great significance to the monitoring of maternal and fetal health during pregnancy and parturition.

The invention belongs to the range of heavy metal ion detection equipment and particularly relates to an optical fiber sensing system for simultaneously and rapidly detecting multiple types of heavy metal ions.The detection system is composed of a light source module (1), a transmission module (2), a sample loading and detecting module (3) and a photoelectric detection module (4) which are sequentially connected; a wavelength-division multiplexing technology in optical fiber communication and novel fluorescent dye quantum dots are adopted for the system, and two light paths are not needed to be separated through an optical loop device.By means of the optical fiber sensing system for simultaneously and rapidly detecting multiple types of heavy metal ions, simultaneous and rapid detection of multiple types of heavy metal ions can be achieved, miniaturization and portability of an instrument are achieved at the same time, and remote detection, real-time analysis, filed detection and dynamic monitoring are achieved; instrument cost is lowered; by means of the system, multiple types of heavy metal ions in a solution can be detected simultaneously and rapidly, and the optical fiber sensing system is widely applied to the fields of environmental detection, industrial sewage treatment, soil heavy metal pollution, food hygiene inspection, medical application and the like.

A self-test auxiliary device and method for visual hardware of a distribution terminal belongs to the technical field of electrical power. The auxiliary device is used for carrying out test wiring of the distribution terminal. A self-test program module is used for automatically complete analog acquisition channel detection, on-off input and output consistency detection, and memory and clock detection for a distribution terminal to be tested. A liquid crystal screen or an LED lamp on the distribution terminal is used for indicating the detection process and the detection result. At the same time, an examination filed is formed and stored in a memory for production technical personnel to consult. For unqualified products, abnormal hardware can be rapidly found according to a self-test file. Periodic self-test of hardware is carried out in the product aging process, the hardware quality problem caused by hardware welding, assembly and the like in the aging process is recorded, automatic testing of distribution terminal hardware is achieved, the test efficiency and the terminal product quality are improved, and the phenomenon that test items are missed due to human factors is avoided.

The invention relates to a quantum dot fluorescent microsphere chromatography kit for simultaneously detecting mixed contamination of aspergillus flavus metabolites cyclopiazonic acid and aflatoxin. The kit comprises a fluorescent chromatography test strip, and a sample reaction bottle containing an anti-cyclopiazonic acid monoclonal lyophilized antibody labeled by the quantum dot fluorescent microsphere and an anti-aflatoxin monoclonal lyophilized antibody labeled by the quantum dot fluorescent microsphere. The anti-cyclopiazonic acid monoclonal antibody is secreted by a hybridoma cell strainYTT-2 with a preservation number of CCTCC NO. C201871. The kit of the present invention can be used for simultaneously detecting cyclopiazonic acid and aflatoxin and has simple and rapid operation and high sensitivity.

The invention discloses a ground surface sound impedence rate measuring device and a ground surface sound impedence rate measuring method. The device comprises a sound wave emission system, a device for detecting a sound pressure level of the ground surface to be tested, and a device for detecting a vibration speed of the ground surface to be tested, wherein the sound wave emission system comprises a signal generator, a sound console, a power amplifier, a loudspeaker, a sound level meter and a computer; the device for detecting the sound pressure level of the ground surface to be tested comprises a sound wave emission system, a sound level meter and a computer; and the device for detecting the vibration speed of the ground surface to be tested comprises a sound wave emission system, a seismic detector array, a multi-channel data acquisition unit and a computer. The sound wave emission system emits high-intensity low-frequency sound waves, the device for detecting the sound pressure level detects a sound pressure level of the ground surface to be tested, the device for detecting the vibration speed of the ground surface detects a vibration speed on the ground surface, and a ratio of the sound pressure level and the vibration speed on the ground surface is a ground surface sound impedence rate. By the ground surface sound impedence rate measuring device and the ground surface sound impedence rate measuring method, the ground surface sound impedence rate can be quickly measured at high accuracy.

The invention discloses a harmonic multi-channel synchronous rapid detection method for a high-speed railway traction power supply system. The problems of poor detection accuracy, poor real-time performance of multi-channel detection and incomplete measured harmonic spectrum of a harmonic detection method of the high-speed railway traction power supply system in the prior art are solved. The harmonic multi-channel synchronous rapid detection method for the high-speed railway traction power supply system comprises multi-channel synchronous sampling, unified artificial neural network reference input reference, an artificial neural network weight adjustment method, multi-channel synchronous parallel processing, multi-channel artificial neural synchronous learning based on a shared knowledge base and additional functions implemented by an algorithm. According to the invention, synchronous rapid detection of multi-channel harmonics in the high-speed railway traction power supply system is completed, which ensures relatively high accuracy and the real-time performance, synchronism and rapidity of multi-channel detection.

The invention relates to a colloidal gold immunochromatographic test strip for synchronously detecting diacetoxyscirpenol, deoxynivalenol and T-2 toxin. The test strip comprises a bottom plate. A water absorption pad, a detection pad, a gold labelled pad and a sample pad are sequentially adhered to one surface of the bottom plate from top to bottom; the adjacent pads are overlapped and connected at the joint; the detection pad takes a nitrocellulose membrane as a base pad; a quality control line and three detection lines are transversely arranged on the nitrocellulose membrane; the three detection lines are positioned under the quality control line and are distributed at intervals, the three detection lines are respectively coated with toxin protein conjugates, and the quality control lineis coated with a rabbit anti-mouse polyclonal antibody; each colloidal gold labeled antibody is transversely sprayed on the gold labeled pad; and the anti-diacetoxyscirpenol monoclonal antibody is secreted by a hybridoma cell strain DAS5G11E7 with the preservation number of CCTCC NO: C201881. The diacetoxyscirpenol, deoxynivalenol and T-2 toxin three mycotoxins can be synchronously and rapidly detected on one test strip.

The disclosed assembly-type multifunction sensor fit for large-scale production comprises: a mobile sensing unit (1) arranged on a sensing energy-conversion platform (2), a control device (3) on side of (3) better on bottom, and an auxiliary unit. This invention also discloses the preparation method.

The invention discloses a preparation method and application of an intelligent antibacterial hydrogel. The method is realized through using vesicles loaded with self-extinguishment fluorescent dyes. The vesicles are sensitive to critical population density adjusting cell virulence factors produced by thalli; and under the circumstance that a wound is infected with bacteria, the produced thallus toxin can cause the breakage of the vesicles, so that the dyes are released from the vesicles, and intense fluorescence is emitted. Therefore, whether the wound is infected or not can be judged according to the fluorescence intensity, and the risk of being infected is further reduced. In addition, the synchronous intelligent response on pathogenic bacterium detection and sterilization can be realized through loading photosensitive antibacterial agents in the vesicles. Compared with a traditional pathogenic bacterium detection method, the preparation method of the intelligent antibacterial hydrogel has the characteristics of simple preparation process, convenience in operation, high efficiency, quickness, accuracy and the like.

The invention discloses a colloidal gold immunoassay test strip for synchronously detecting aspergillus flavus fungaltoxin as well as preparation and application thereof. The colloidal gold immunoassay test strip comprises a bottom plate, wherein an absorbent pad, a detection pad, a gold label pad and a sample pad are stuck to one side of the bottom plate from top to bottom in sequence; adjacent pads are connected at joints in an overlapping manner; the detection pad takes a nitrocellulose membrane as a base pad; a quality control line and detection lines are transversely arranged on the nitrocellulose membrane from top to bottom; three detection lines are positioned below the quality control line, and distributed at intervals; the three detection lines are coated with toxin protein conjugates respectively; the quality control line is coated with a rabbit anti-mouse polyclonal antibody; and an anti-cyclopianic acid monoclonal antibody is generated by secretion of a hybridoma cell strain YTT-2 of which the collection number is CCTCC NO.C201871. The colloidal gold immunoassay test strip can be applied to the synchronous detection of aflatoxin, sterigmatocystin and cyclopiazonic acid,is simple and rapid in operation, and has high sensitivity.

The invention belongs to the technical field of microbiological detection and particularly relates to a self-driven micro-fluidic chip with integrated processing and amplified color developing functions. The chip is formed by combining a plurality of pathogen detecting monomers; sample liquid pools on the pathogen detecting monomers communicate with one another through channels; and the channels are constructed by nanometer materials and by a shielding technology. The self-driven micro-fluidic chip can perform amplification directly from tissue lysis fluid at one step, can effectively reduce the sample detection cost, can improve the detection efficiency, can guarantee the detection accuracy and can improve the epidemic disease screening ability of shrimp seed industry fundamentally.

The invention relates to a roll-to-roll surface rapid detection system for a stainless steel substrate of a CIGS battery piece, and solves the problems that the surface quality detection of a stainless steel substrate coiled material mainly depends on naked eye observation and judgment of workers and the working efficiency is low. A plurality of conveying rollers for conveying a substrate coiled material are arranged between an unwinding shaft and a winding shaft, a surface detection system is arranged between every two adjacent conveying rollers and comprises a laser range finder, a spectroscope is arranged between the laser range finder and the surface of the substrate coiled material, and the spectroscope divides laser into a transmission light beam and a reflection light beam; the transmission light beam forms point projection on the surface of the substrate coiled material, the reflection light beam forms line projection on the surface of the substrate coiled material after passing through the beam expander and the 45-degree conical mirror, and the surface defect detection camera is arranged above the line projection. Synchronous detection of the tension degree, flatness and oil stain scratch defects of the stainless steel substrate coiled material is achieved through the same light source, and the defects of the substrate coiled material can be automatically and rapidly detected.