Preparation method and application of intelligent antibacterial hydrogel

A hydrogel, intelligent technology, applied in the field of biosensing, can solve problems such as the imbalance of drug-resistant microbial flora, and achieve good biocompatibility

Inactive Publication Date: 2020-02-04
GUANGXI UNIV
View PDF5 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, antibiotic antibacterial agents are widely used. Hydrogels achieve the purpose of anti-infection by releasing antibiotics, interfering with the important life processes of bacteria, directly killing them or inhibiting the growth of bacteria, but due to the rapid adaptability of bacteria and evolutionary ability, extremely prone to negative effects such as drug resistance and microbial flora imbalance

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method and application of intelligent antibacterial hydrogel
  • Preparation method and application of intelligent antibacterial hydrogel
  • Preparation method and application of intelligent antibacterial hydrogel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1. A kind of intelligent antibacterial hydrogel

[0030] The application of intelligent antibacterial hydrogel in the detection of pathogenic bacteria, the operation steps are as follows:

[0031] 1. Enzymatic reaction in solution

[0032] For the enzymatic reaction in the solution, 50 μL MUD solution was added to a 96-well plate containing 50 μL bacterial solution, the 96-well plate was sealed with sealing tape, and the fluorescence intensity was observed in the fluorescent cell. figure 1 shown.

[0033] 2. Enzymatic reactions of hydrogel dressings on Petri dishes

[0034] Graft 50 μL of MUD solution on the surface of the prepared hydrogel dressing, and then put it on a petri dish coated with 50 μL of bacterial solution, and observe the luminous intensity of the hydrogel dressing as follows: figure 2 shown.

[0035] 3. Preparation of hypericin inclusion complex

[0036] Add 5.0 g of β-CD (β-cyclodextrin) into 10 ml of 30% NaOH solution, stir at room te...

Embodiment 2

[0042] The application of intelligent antibacterial hydrogel in intelligent antibacterial experiments, the operation steps are as follows:

[0043] 1. Bacterial culture

[0044] Pick the bacteria from the LB plate, put them in a 50ml centrifuge tube, add 10ml of LB culture solution to the centrifuge tube, and culture it in a shaker at 37°C for 15h, and set aside.

[0045] 2. Preparation of solid medium

[0046] Take a 1000ml beaker, put it into 500ml distilled water, weigh 5g beef extract, 10g peptone, 5g agar, and 5g sodium chloride in water, adjust the pH to about 7.2 with 10% NaOH solution, and adjust the volume to 1000ml. Wrap it well, sterilize it in a sterilization box, and set it aside.

[0047] 3. Antibacterial experiment

[0048] Dilute the cultured bacteria 10 times with LB solution and measure the OD 600 value, then dilute the strain to 0.01 for later use. Take out the 96-well plate, add bacterial solution and photosensitive antibacterial agent, incubate in an in...

Embodiment 3

[0053] The application of smart antibacterial hydrogel on the effect of smart photosensitive antibacterial agent on mouse wound healing, the operation steps are as follows:

[0054] 1 Experimental materials

[0055] 1.1 Experimental reagents

[0056] Hypericin or natural photosensitive antibacterial agent extract, medical alcohol, diluted bacterial liquid

[0057] 1.2 Experimental animals

[0058] Kunming mice

[0059] 2 Experimental methods

[0060] 2.1 Establishment of mouse wound model

[0061] Take a healthy mouse, use urethane to anesthetize it, take clean scissors, remove the mouse hair, disinfect the haired place with medical alcohol, and then use the sterilized scissors and tweezers to make an area of ​​about It is a 1cm2 wound model.

[0062] 2.2 Grouping and administration

[0063] The mice were divided into experimental group and control group, with 10 mice in each group. In the experimental group, bacteria were applied to the wounds of the mice and smart hyd...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
thicknessaaaaaaaaaa
Login to view more

Abstract

The invention discloses a preparation method and application of an intelligent antibacterial hydrogel. The method is realized through using vesicles loaded with self-extinguishment fluorescent dyes. The vesicles are sensitive to critical population density adjusting cell virulence factors produced by thalli; and under the circumstance that a wound is infected with bacteria, the produced thallus toxin can cause the breakage of the vesicles, so that the dyes are released from the vesicles, and intense fluorescence is emitted. Therefore, whether the wound is infected or not can be judged according to the fluorescence intensity, and the risk of being infected is further reduced. In addition, the synchronous intelligent response on pathogenic bacterium detection and sterilization can be realized through loading photosensitive antibacterial agents in the vesicles. Compared with a traditional pathogenic bacterium detection method, the preparation method of the intelligent antibacterial hydrogel has the characteristics of simple preparation process, convenience in operation, high efficiency, quickness, accuracy and the like.

Description

technical field [0001] The invention belongs to the technical field of biosensing, in particular to a preparation method and application of an intelligent antibacterial hydrogel. Background technique [0002] With the widespread application of antibiotics in clinical practice, the problem of bacterial drug resistance is becoming more and more serious, and its drug resistance is increasing. pose great challenges. At present, the detection of pathogenic microorganisms and their drug resistance is mainly accomplished by analytical methods such as bacterial culture, biochemical identification, polymerase chain reaction amplification, real-time fluorescent quantitative gene amplification technology, enzyme-linked immunoassay and chip technology. The above-mentioned detection methods have problems such as long detection cycle, high cost, and cumbersome operation, which are difficult to meet the needs of clinical real-time detection. Therefore, the development of rapid and sensit...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61L26/00C12Q1/18C08J3/075G01N21/64C08L5/08C12R1/385C12R1/19C12R1/445
CPCA61L26/0023A61L26/0061A61L26/0066A61L26/008A61L2300/216A61L2300/404A61L2300/626C08J3/075C08J2305/08C12Q1/18C12Q2334/22G01N21/6402C08L5/08
Inventor 刘海波周倩王静李青云刘幽燕廖丹葵
Owner GUANGXI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap