The invention discloses a preparation method of baits for improving the fishing efficiency. The preparation method comprises the following steps of uniformly mixing pearl barley, oat powder, sesame seed meal, fermented tea seed meal, fermented peanut meal, corncob powder, straw powder and fermented glutinous rice, then standing, adding an EM (effective microorganism) bacterium solution, uniformly mixing, putting into a freshness protection package, and fermenting to obtain a mixed material A; performing cold air drying on blood worms, earthworms, whitebait, freshwater shrimps, cicada pupa, cadelle, fly maggots, bean worms, moina, minced fillet and snails, and then smashing to obtain a mixed material B; mixing anise, rhizoma polygonati, fennel, elsholtzia, tuckahoe, hawthorn, dried orange peel, spina date seed, liquorice, angelica sinensis, honeysuckle and radix bupleuri, and then smashing to obtain traditional Chinese medicine powder; adding the traditional Chinese medicine powder into rice wine, uniformly mixing, and then standing for 3 to 4 days, so as to obtain a mixed material C; uniformly mixing the mixed material A, the mixed material B and the mixed material C, then spraying, performing mechanical pressure accumulated processing, and then drying, so as to obtain the baits for improving the fishing efficiency.