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Method for co-producing ethanol, acetone and butanol by lignocellulose biomass

A lignocellulose and biomass technology, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of industrial decay of acetone and butanol fermentation, and achieve the increase of organic solvents, the enhancement of blasting effect, and the saving of energy. effect of investment

Active Publication Date: 2015-07-15
SHANDONG LONGLIVE BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the rapid development of the petrochemical industry, the acetone and butanol fermentation industries in various countries have declined all the way

Method used

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  • Method for co-producing ethanol, acetone and butanol by lignocellulose biomass
  • Method for co-producing ethanol, acetone and butanol by lignocellulose biomass

Examples

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Embodiment 1

[0043] The present invention will be further described below in conjunction with the drawings and embodiments.

[0044] Such as figure 1 As shown, a method for co-producing ethanol, acetone and butanol from corn stover specifically includes the following steps:

[0045] 1. Cut corn stalks to 3cm, put them in 3 times the mass of 10% (w / w) ammonia solution, soak at room temperature for 3 hours, filter to separate the solid and liquid, and use the liquid to soak the next batch of materials; place the solid in the blasting machine (QBS-200B, Hebi Zhengdao Bioenergy Co., Ltd., Henan), 2.5MPa hold pressure for 60s, instantaneous depressurization sprays out blasting slag; the blasting slag is placed in 4 times the mass of water, and the pH is adjusted to 5.5 with sulfuric acid.

[0046] 2. Activation and fermentation of cellulase from Penicillium oxalicum

[0047] (1) Strain activation: Penicillium oxalicum was grown on a PDA plate at 30°C for 7 days, and then a single colony was picked and...

Embodiment 2

[0074] Such as figure 1 As shown, a method for co-producing ethanol, acetone and butanol from corn stover specifically includes the following steps:

[0075] 1. Cut corn stalks to 5cm, place 4 times the mass in 15% (w / w) ammonia solution, soak at room temperature for 30 minutes, filter to separate the solid and liquid, and use the liquid to soak the next batch of materials; place the solid in the blasting machine (QBS-200B, Hebi Zhengdao Bio-Energy Company, Henan), 1.8MPa hold pressure for 350s, instantaneously reduce the pressure and spray out the blasting slag; the blasting slag is placed in 3 times the mass of water, and the pH is adjusted to 5 with sulfuric acid.

[0076] 2. Activation and fermentation of cellulase from Penicillium oxalicum

[0077] (1) Strain activation: Penicillium oxalicum was grown on a PDA plate at 30°C for 7 days, and then a single colony was picked and streaked on agar containing PDA (potato powder: 5.0g / L, glucose: 20.0g / L , Agar: 15.0g / L, chloramphenic...

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Abstract

The invention discloses a method for co-producing ethanol, acetone and butanol by lignocellulose biomass. The method comprises the following steps: (1) crushing a lignocellulose material, and placing the crushed lignocellulose material in ammonia water solution and soaking to obtain a mixture; (2) carrying out solid-liquid separation on the mixture obtained in the step (1), and carrying out an explosion treatment on solids to obtain explosion residues; (3) placing the explosion residues in water, uniformly mixing, adding enzyme solution, saccharifying, inoculating the activated saccharomyces cerevisiae solution, and carrying out synchronous saccharifying fermentation; (4) then inoculating the activated clostridium beijerinckii solution in a fermentation tank, and fermenting to obtain fermentation solution; and (5) carrying out reduced pressure distillation on the fermentation solution to obtain crude ethanol, acetone and butanol, and further rectifying and dehydrating to obtain absolute ethanol, acetone and butanol. Through the method disclosed by the invention, the ratio of the three products of ethanol, acetone and butanol is reasonable; because the alcohol content is high due to high-concentration material saccharifying, and the by-products of ABE fermentation can be separated from butanol simultaneously, the distillation cost is reduced due to the increase of organic solvents.

Description

Technical field [0001] The invention relates to a method for comprehensive utilization of lignocellulosic biomass, in particular to a method for co-producing ethanol, acetone and butanol from lignocellulosic biomass. Background technique [0002] Since the era of the industrial revolution, the economy has developed rapidly based on the rapid consumption of fossil energy represented by petroleum and coal. China's oil reserves account for only 2% of the world's total, but its annual consumption ranks second in the world. The amount of oil that can be exploited in the world is getting less and less, and China's economic development has led to an increasing consumption of oil. In 2011, China’s net crude oil imports exceeded 200 million tons for the first time, and its dependence on foreign sources exceeded 55%. In 2012, China’s dependence on crude oil imports rose to 58%. Experts predict that the current global oil reserves can only maintain consumption for about 100 years, and if ...

Claims

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Application Information

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IPC IPC(8): C12P7/10C12P7/16C12P7/28C12R1/865
CPCC12P7/10C12P7/16C12P7/28C12P19/14C12P2201/00C12P2203/00Y02E50/10
Inventor 程少博肖林夏蕊蕊王新明覃树林
Owner SHANDONG LONGLIVE BIO TECH CO LTD
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