The invention belongs to the technical field of
natural product extraction, and particularly relates to extraction and
derivatization of water-soluble apple
polysaccharide and application of the water-soluble apple
polysaccharide in cigarettes. The extraction method of the water-soluble apple
polysaccharide comprises the following steps: freezing and centrifuging
apple pulp, filtering to obtain a solution A, precipitating with 95%
ethanol, freezing and centrifuging overnight, filtering to obtain an
alcohol-insoluble substance B,
drying under reduced pressure until no
alcohol smell exists, dispersing in a proper amount of water to obtain a solution B, concentrating to a proper amount, performing deproteinization treatment, stirring, standing, centrifuging, and separating out supernate, thereby obtaining the water-soluble apple polysaccharide. And carrying out
column chromatography treatment to obtain a solution D, carrying out membrane separation treatment by using filter membranes with different pore diameters, concentrating, and freeze-
drying to obtain the refined apple polysaccharide. In a
solution state, the apple polysaccharide can form a
stable system with certain
viscosity. Meanwhile, the apple polysaccharide also has certain
oxidation resistance, and can
delay the
oxidation process of the
system to a certain extent and maintain the stability of the components.