24 results about "Fluorescent cell" patented technology

The invention belongs to the technical field of biology, and discloses a stable protective agent, a cell antigen dry sheet and a preparation method and application of the dry sheet, the stable protective agent comprises glycerin with the volume fraction being 0.4%, gelatin with the mass volume fraction being 0.1%, EDTA with the mass volume fraction being 0.1 mM, trehalose with the mass volume fraction being 0.03% and resveratrol with the mass volume fraction being 100 [mu] M. The stable protective agent is adopted for preparing the cell antigen dry sheet, and the cell antigen dry sheet is applied to CBA detection. The stable protective agent has good permeability, can penetrate through cell membranes to enter cells, provides a protection effect inside and outside the cells, has moisture retention and oxidation resistance, can prevent cell deformation and cell fixing layer shedding, and can well maintain the stability and reliability of antigen proteins, and the prepared cell antigen dry sheet can be stored for a long time, and has a good application prospect. The antigenicity of a membrane signal index is not damaged. When a positive sample is detected, obvious green fluorescence cells can be seen, red fluorescence quenching is avoided, and co-localization of red fluorescence and green fluorescence can be observed conveniently.

The invention relates to the technical field of biological materials, in particular to a biocompatible method for synthesizing quantum dots from living cells, which comprises the following steps: in the living cell culture process, adding an organic selenium compound containing Se-Se or Se-S bonds into a culture medium, and incubating to obtain selenized cells; further culturing by using a culture medium containing a metal ion-glutathione complex to obtain quantum dots containing metal elements and Se elements; according to the method, the generation of an active selenium precursor with cytotoxicity, such as hydrogen selenide, is effectively avoided, and meanwhile, the outbreak of active oxygen species in cells is also avoided, so that high quantum dot synthesis efficiency is shown. In-vitro cell experiment results show that the cells are endowed with a fluorescent function and still maintain good cell viability, and the cell viability is maintained at 80% or above; in-vivo experiment results show that the fluorescent cells with the synthesized quantum dots have good proliferation, tumor formation, invasion and metastasis capabilities in vivo.

The invention discloses application of a multi-fungus fluorescent staining solution in preparation of a kit for rapidly identifying survival / drug resistance of fungi, and belongs to the technical field of biological detection. The kit can be used for identifying the death and activity of fungi and high-precision rapid drug resistance identification while specific fungi staining is carried out; a fluorescence brightening agent is adopted to mark fungal components in a sample, and the fungal form is clearly observed under a fluorescence microscope. The nucleic acid fluorescent dye capable of selectively permeating and damaging the cell membrane displays the dead and living state of fungi on the basis of fungus dyeing, and the fluorescent dye permeates into cells and is combined with nucleic acid to emit fluorescence. Metabolic reactive dyes of the cells are used for carrying out rapid drug resistance identification on fungi subjected to drug action. The method can simultaneously realize fungal death and activity identification and rapid drug resistance detection, and is simple and convenient to operate, high in detection speed and accurate in result. The kit is suitable for rapid auxiliary diagnosis and treatment of clinical fungal infection. When the multi-fungus fluorescent staining solution is used for preparing the drug sensitivity detection kit, the detection time of the kit is only 1-2 hours.

The invention provides a method for quantitatively analyzing immune response strength promoted by an adjuvant and application of the method in adjuvant screening, and relates to the technical field of vaccine adjuvants. According to the method for quantitatively analyzing the immune response strength promoted by the adjuvant, the immune response level of the to-be-detected adjuvant can be predicted only by taking the control adjuvant as a reference substance and taking the number of KikGR red fluorescent cells 6-120 hours after immunostimulation of the combined antigen as an index. The method has the characteristics of noninvasive and continuous tracing, simplifies the flow and period of adjuvant effect evaluation, improves the screening efficiency, reduces the research and development period, and provides an effective technical means for rapid screening of adjuvants.

The invention provides a dyeing method for removing autofluorescence of a single cell sorting micro-fluidic chip and a chip without autofluorescence. The dyeing method comprises the following steps: completely soaking the single-cell sorting micro-fluidic chip in a dye solution for dyeing until the top layer of the chip is dyed into a non-transparent black top layer, thereby obtaining the single-cell sorting micro-fluidic chip without autofluorescence, the dye in the dye solution is black dye; the single cell sorting micro-fluidic chip at least comprises a photosensitive dry film serving as a runner layer. The staining method provided by the technical scheme of the invention can effectively solve the problem of autofluorescence of a single cell sorting micro-fluidic chip prepared from a traditional photosensitive dry film, so that when the stained chip is applied to fluorescence activated cell sorting, recognition of automatically screened fluorescent cells is facilitated, the operation difficulty of fluorescence activated cell sorting is reduced, and the working efficiency is improved. And accuracy and reliability are improved.

The application discloses a double-color fluorescence detection anti-NMDAR antibody fixing reagent, a method and application thereof, and the fixing reagent comprises a mixture of paraformaldehyde, methanol and acetone, wherein the volume fraction of methanol in the mixture is 10-25%, and the volume fraction of acetone is 75-90%; the fixing effect is better when the fixing is carried out in two steps by using 4% paraformaldehyde in mass-volume concentration and the mixture of 15% methanol and 85% acetone in volume fraction. By using the fixing liquid and the fixing method, the antigenicity of NMDAR is not damaged, obvious green fluorescent cells can be observed in positive samples, red fluorescence is not quenched, and co-localization of red fluorescence and green fluorescence can be observed by using a fluorescence microscope.

The application provides a fluorescence cell multilayer microscopic imaging method and system based on multi-feature fusion, and relates to the technical field of microscopic imaging. The method comprises the following steps: acquiring a multilayer Z-axis image sequence of a fluorescence cell sample at an X-Y scanning point; dividing an optimal focal plane image into blocks, and identifying the definition state through a convolutional neural network classification model; searching for a replacement block in a neighboring Z layer according to the classification result for a fuzzy block; and outputting a full field of view image after local registration and feathering fusion. The application solves the problems of unstable automatic focusing and insufficient single focal plane coverage under weak fluorescence conditions by fusing multi-feature definition evaluation and deep learning block classification, and realizes full field of view clear imaging of a fluorescence sample with uneven thickness.

The application belongs to the technical field of biology, and discloses a stabilizing protective agent, a cell antigen dry sheet, and a preparation method and application of the dry sheet. The stabilizing protective agent comprises 0.4% glycerol in volume fraction, 0.1% gelatin in mass volume fraction, 0.1 mM EDTA, 0.03% trehalose in mass volume fraction, and 100 muM resveratrol. The stabilizing protective agent is used to prepare the cell antigen dry sheet and is applied in CBA detection. The stabilizing protective agent has good permeability, penetrates into the cell interior through the cell membrane, provides protection inside and outside the cell, has moisturizing and antioxidant properties, can prevent cell deformation and cell fixation layer from falling off, well maintains the stability and reliability of antigen protein, and the prepared cell antigen dry sheet can be stored for a long time without damaging the antigenicity of the membrane signal index. When a positive sample is detected, obvious green fluorescent cells can be seen, and red fluorescent quenching does not occur, so that the co-localization of red fluorescence and green fluorescence can be observed.

The purpose of the present invention is to provide an extracellular vesicle, a preparation method thereof, and a typing analysis method. By removing the surface N-sugar of the extracellular vesicles, the uptake rate of the extracellular vesicles by specific cells is increased. This technology uses co-culture of living cells and extracellular vesicles, fluorescent labeling, and flow cytometry to detect fluorescent cells and fluorescence intensity in cell suspensions in different cell culture dishes. The interaction between living cells and extracellular vesicles is used to perform typing analysis and verification of the extracellular vesicles. In addition, the invention provides a method for preparing extracellular vesicles that can be more efficiently taken up by HCT116.

1. Name of the Design Product: Inverted Fluorescence Cell Imager. 2. Use of the Design Product: It is used for an inverted fluorescence cell imager, which is used for real-time fluorescence observation and measurement of living cells in biological and medical research. 3. Design Key Points of the Design Product: Lies in the shape. 4. Picture or Photo that Best Illustrates the Design Key Points: Perspective view.

The invention discloses a head and neck cancer chemotherapy recurrence animal model and a construction method and application thereof, and relates to the technical field of animal model construction. Comprising the following steps: S1, culturing head and neck cancer tumor cells with fluorescence labels under an in-vitro condition; s2, subcutaneously planting the head and neck cancer tumor fluorescent cells in a groin part of a model animal; s3, 5 days after planting, imaging and observing subcutaneous tumor formation of the model animal, and performing periodic chemotherapy; s4, monitoring through a living body imaging system until a tumor fluorescence signal basically disappears, and determining tumor regression; s5, stopping chemotherapy, continuing feeding for 5-6 weeks, and observing the tumor recurrence condition of the model animal to obtain the head and neck cancer chemotherapy recurrence animal model. The method is easy and convenient to operate, short in experimental period and low in consumption, non-invasive dynamic monitoring can be achieved through the living body imaging technology, the variability of the result is small, the success rate is high, and an ideal platform is provided for head and neck cancer recurrence research and drug screening.

A method of identifying a fluorescent cell in a sample using a microfabricated chip having a top surface including a plurality of microwells. At least one cell of a sample is loaded into at least one microwell of the plurality of microwells. The microfabricated chip is incubated to grow a population of cells from the at least one cell in the at least one microwell. Fluorescence exhibited by at least one microwell is detected by analyzing an image of the microfabricated chip, to thereby determine a presence of a fluorescent cell of interest in the sample.

The invention discloses a biological coenzyme resistant reduced methylene blue modified photosensitizer as well as a preparation method and application thereof. The photosensitizer is modified by introducing an electron-donating alkyl group into a methylene blue side chain. The preparation method comprises the following steps: treating specific aniline and benet salt in strong acid by using an oxidizing agent to obtain an intermediate, and reacting in an organic solvent under the action of a catalyst and the oxidizing agent to generate a target product. The photosensitizer can maintain continuous and efficient photodynamic activity in a biological environment, it is guaranteed that active oxygen is efficiently generated under illumination due to the biological coenzyme reduction resisting characteristic of the photosensitizer, and the ROS generation performance is remarkably improved. Through modification, the oil-water distribution coefficient is increased, the hydrophilicity is reduced, the fluorescent powder is more easily taken by cells, bright fluorescence can be observed in a short incubation time, and an intracellular fluorescence signal is greatly enhanced. The photosensitizer realizes effective improvement of the cell killing ability, shows excellent tumor inhibition ability in a tumor model, remarkably improves the tumor inhibition rate, and has excellent tumor phototherapy application potential.

A touch display panel having a plurality of light emitting elements; a plurality of color filters formed at positions corresponding to the plurality of light emitting elements; and an infrared fluorescent pattern comprising a plurality of individual infrared fluorescent cell patterns formed at positions corresponding to at least some of the plurality of color filters; and an infrared control film positioned on a light emitting surface of the display panel.

The invention discloses a fluorescence report system for evaluating homologous mediated DNA (deoxyribonucleic acid) repair efficiency in rice cells. According to the reporting system, plasmids for coding CRISPR / Cas and repairing template DNA are delivered into rice cells, so that a nucleotide sequence for coding a fluorescent protein gene is inserted into the tail end of a coding region of a rice histone gene OsH2B (LOCOs01g62230) with high expression quantity at a fixed point through a homologous mediated DNA repairing mechanism, and frame-combined protein coding sequence fusion is formed; according to the method, the cells subjected to precise editing have the property of emitting fluorescence, the proportion of the cells emitting the fluorescence is detected by utilizing flow cytometry (FACS), and the homologous mediated DNA repair efficiency in the rice cells is calculated. The method effectively solves the technical bottlenecks of high cost, long period and complex analysis in evaluation of editing efficiency depending on deep sequencing, can be used for developing a precise gene editing technology based on homologous mediation DNA repair and the like, and has the characteristics of low cost, short period, simplicity and convenience in analysis and the like.

The present disclosure relates to an intelligent event imaging flow cytometer, comprising a microfluidic system, an optical system and a hardware processing system. The microfluidic system is used for continuously importing a cell sample comprising at least two kinds of cells, and exporting each kind of cell according to the classification result fed back by the hardware processing system. The optical system is used for jointly irradiating the cell sample by a bright field light source and a fluorescent light source, and collecting multiple double-channel coupling information simultaneously comprising bright field cells and fluorescent cells. The hardware processing system is used for obtaining the classification result of the target cell according to multiple target coupling information corresponding to the target cell determined in the double-channel coupling information, and feeding back the classification result to the microfluidic system. In the present disclosure, the cell classification in the cell sample can be realized through the information interaction transmission of the three systems, which simplifies the overall structure of the cytometer. At the same time, the cell classification result is improved in accuracy by jointly judging the cell classification through the cell characteristics collected under the bright field and fluorescent light sources.

The invention discloses an immune cell killing activity detection method based on fluorescently labeled tumor target cells, and relates to the technical field of detection.The immune cell killing activity detection method comprises the steps that peripheral blood or umbilical cord blood of a healthy volunteer is collected, mononuclear cells are obtained through separation with a human lymphocyte separation medium and induced into immune effector cells, and multiplication culture is conducted; taking the fluorescently-labeled tumor cells as target cells, and co-incubating the immune effector cells and the target cells for a period of time; collecting a cell product after co-incubation is finished, and detecting the number of fluorescently-labeled positive cells in the co-incubation product by using a flow cytometer or a fluorescent cell counter; and calculating the killing rate of the immune effector cells to the target cells based on the detected number of the positive cells. The scheme is simple and convenient to operate, rapid, accurate, low in cost and free of cell damage.

The invention provides a detection method and a detection device for multicellular clusters in circulation. The detection method specifically comprises the following steps: exciting and receiving fluorescent pulse signals when fluorescent cells flow through a detection area; the exciting light is focused on an interested blood vessel through a microscope objective; when the fluorescently-labeled cells flow through the laser detection part, fluorescence is released; converting into an electric signal through a photoelectric conversion device; the fluorescence pulse signal subsequently enters a signal conversion device; signal conversion is carried out through a comparator; and when the fluorescence trigger signal is transmitted to the imaging device, the lighting equipment is turned on, and the imaging device records an image sequence of the cells flowing through the detection area after receiving the fluorescence trigger signal. According to the detection device and the detection method for the multicellular clusters in circulation, the physiological environment of the human body can be efficiently, quickly and accurately detected, in-vitro operation such as blood sampling and centrifugation is not needed, a detection target does not leave the living body environment, and a detection result can better reflect the real situation in the physiological environment.

The present invention relates to a cell-based method for detecting botulinum toxin or measuring the activity thereof, and relates to: a genetically modified cell into which a gene construct consisting of an anchor protein group and a dim fluorescent peptide group is introduced; and a use thereof, and, more specifically, to: a genetically modified cell into which a gene construct consisting of a cell membrane-binding peptide and a recombinant fluorescent protein coding sequence, which form an anchor protein group, and a botulinum toxin recognition sequence and a dim fluorescence-inducing peptide coding sequence for fluorescent protein expression, which form a dim fluorescence-inducing peptide group, is introduced; and a use thereof. According to the method of the present invention, botulinum toxin can be detected or the activity thereof can be quantitatively analyzed through fluorescence measurement without using cell lines that are difficult to culture, such as stem cells.

The invention discloses a virus protein dynamic monitoring system and method based on a fluorescent gene editing technology, and relates to the technical field of fluorescent tracer, the system comprises a gene editing module, a fluorescent gene and a Tax response promoter are used for constructing a co-transcription unit by using CRISPR / Cas9, the co-transcription unit is inserted into a virus genome non-functional region through homologous recombination repair, and the gene editing module is used for editing the gene. Subculturing and screening a stable cell line with the fluorescence positive rate reaching the standard; the signal response module is used for realizing synchronous expression of fluorescent protein and Tax through a co-transcription mechanism; the cell carrier module simulates infection through co-culture; the detection and analysis module quantifies the fluorescence intensity and the number of fluorescent cells through a fluorescence microscope and a flow cytometer, the detection period is shortened, the detection efficiency is improved, the system does not need antibiotic treatment, and Tax dynamics can be monitored in real time.

A method for identifying fluorescent cells in a sample using a microfabricated chip having a top surface comprising a plurality of microwells. At least one cell of the sample is loaded into at least one microwell of the plurality of microwells. The microfabricated chip is incubated to allow the at least one cell in the at least one microwell to grow into a cell population. The presence of a fluorescent cell of interest in the sample is determined by analyzing an image of the microfabricated chip to detect fluorescence exhibited by the at least one microwell.

The application belongs to the technical field of biotechnology, and particularly relates to a recombinant vector, a cell, a kit and a method for screening of a host factor dependent on influenza virus polymerase activity. The application provides a recombinant vector, which is configured with an mScarlet reporter gene through a double tandem strategy (PB1-PB2, PA-NP), and a red fluorescent cell line stably expressing H5N1 polymerase driven by the red fluorescent cell line can be efficiently constructed by using only three lentiviral vectors. Compared with a traditional co-transduction method of five vectors, the difficulty of multiple vectors entering the same cell is greatly reduced, and the recombinant vector does not need live virus and can be stably passaged for a long time. Based on the recombinant vector, a host factor affecting polymerase activity is screened by using a whole genome CRISPR under a non-infectious state, and the method has the advantages of simple operation, high throughput and low cost.

The present invention provides a fluorescent staining method for live-cell imaging. First, cells are double stained through the first and second fluorescent biomarker. Then, the clear fluorescent cell image is shown under a fluorescent microscope, and we can observe the nucleus form while observing the cell form through the obtained image.