Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of triple responsive nanogel and application of triple responsive nanogel

A nanogel, triple-response technology, applied in nanotechnology, preparations for in vivo tests, medical preparations with inactive ingredients, etc., can solve difficult imaging, high cost, complex preparation process of stimuli-responsive nanogels, etc. problem, to achieve good biocompatibility, low hazard effect

Inactive Publication Date: 2017-06-23
UNIV OF SCI & TECH BEIJING
View PDF3 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, Yan Deyue et al. reported poly(diselenide-phosphate) self-assembled nanogels for cancer therapy and HePeixin et al. studied temperature, reducing species and pH triple stimulus response poly(N-isopropylacrylamide- Co-acrylic acid) nanogels are used in the delivery of anticancer drugs. The two research groups have successfully synthesized stimuli-responsive nanogels and applied them to drug delivery and release in vitro. However, due to the defects of the materials themselves, it is difficult to achieve cell Inner Imaging
[0004] At present, the preparation process of stimuli-responsive nanogels is complicated, the cost is high, the controlled release conditions are single, and it is difficult to image in cells through the fluorescence of the material itself.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of triple responsive nanogel and application of triple responsive nanogel
  • Preparation method of triple responsive nanogel and application of triple responsive nanogel
  • Preparation method of triple responsive nanogel and application of triple responsive nanogel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Follow the steps of the acylation reaction to prepare spiropyranyl methacrylate. Take 300 mg of spiropyran, 24 microliters of methacryloyl chloride and 40 microliters of triethylamine, dissolve them in 20 milliliters of tetrahydrofuran (the filling amount of tetrahydrofuran is 40% of the capacity of the container), and add the mixed solution to the dry In a container, stirred at room temperature for 20 hours, the solution turned orange with precipitation, and then the solution was filtered three times to remove the precipitation, and then the saturated sodium bicarbonate weak alkaline solution and dichloromethane were 1 according to the volume ratio: 3 After mixing, add it to the solution that was filtered to remove the precipitate, and extract it in a separatory funnel until the solution becomes colorless, then extract it three times with deionized water, and add excess magnesium sulfate to the final solution, Stir for 15 hours to remove residual water in the solution....

Embodiment 2

[0032] Follow the steps of the acylation reaction to prepare spiropyranyl methacrylate. Take 400 mg of spiropyran, 55 microliters of methacryloyl chloride and 45 microliters of triethylamine respectively, dissolve them in 15 milliliters of tetrahydrofuran (the charging amount of tetrahydrofuran is 30% of the capacity of the container), and add the mixed solution to the dry In a container, stirred at room temperature for 30 hours, the solution turned orange with precipitation, and then filtered the solution three times to remove the precipitation, then mixed the weakly alkaline solution and dichloromethane at a volume ratio of 1:4, and added into the solution that was filtered to remove the precipitate, and extracted in a separatory funnel until the solution became colorless, then extracted three times with deionized water, added an excessive amount of magnesium sulfate in the finally obtained solution, stirred for 20 hours, and used To remove residual water in the solution, th...

Embodiment 3

[0038] Follow the steps of the acylation reaction to prepare spiropyranyl methacrylate. Take 350 mg of spiropyran, 50 microliters of methacryloyl chloride and 60 microliters of triethylamine, dissolve them in 30 milliliters of tetrahydrofuran (the charging amount of tetrahydrofuran is 60% of the capacity of the container), and add the mixed solution to the dry In a container, stirred at room temperature for 25 hours, the solution turned orange with precipitation, then filtered the solution three times to remove the precipitation, then mixed the weakly alkaline solution and dichloromethane at a volume ratio of 1:2, and added into the solution that was filtered to remove the precipitate, and extracted in a separatory funnel until the solution became colorless, then extracted three times with deionized water, added an excessive amount of magnesium sulfate in the finally obtained solution, stirred for 10 hours, and used To remove residual water in the solution, the solution after ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a preparation method of triple responsive nanogel and application of the triple responsive nanogel, which belong to the technical field of macromolecular materials, and relate to preparation and application of polymer nanogel. The preparation method comprises the following steps: adding spiropyrane methacrylate prepared by virtue of an acylation reaction, acrylic acid, N,N-bis(acrolyl) cystamine and lauryl sodium sulfate according to a molar ratio of 2: 30: 1: (1 to 2): 50: 1: 1 into a container fully filled with nitrogen, reacting for a plurality of hours at a certain temperature, and freeze drying to obtain the triple responsive polyacrylic acid-co-spiropyrane methacrylate nanogel. By adopting the preparation method, the carrying of the nanogel for guest molecules is realized, and the controlled release under different single or cooperative out-field irritation of light, pH and a reduction substance (DTT) is realized; and meanwhile, the preparation method is simple, lowcost, capable of being well adaptive to complicated living body environment and capable of being used for biological fluorescent cell imaging treatment and drug delivery.

Description

technical field [0001] The invention belongs to the technical field of polymer materials, and relates to the preparation and application of polymer nanogels. Background technique [0002] Nanogels are three-dimensional cross-linked polymer networks of submicron size and are composed of hydrogel particle entities with nanometer-sized spaces, so they have both the above-mentioned characteristics of hydrogels and nanoparticles. Polymer nanogels are composed of hydrophilic polymers or environmentally sensitive polymers, which expand or contract in response to the environment. Stimuli-responsive polymer nanogels are nanogels that can respond to different external stimuli, so they are also called smart nanogels. External stimuli usually include: changes in ambient temperature, pH of the dispersion medium, light, magnetic field, specific chemical substances, and ionic strength. [0003] In recent years, domestic and foreign research groups have conducted extensive research on sin...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C08F220/06C08F220/36C08F222/38A61K49/00A61K9/06A61K47/32A61K31/704A61P35/00B82Y40/00
CPCA61K9/06A61K31/704A61K47/32A61K49/0073B82Y40/00C08F220/06C08F220/36C08F222/385
Inventor 王国杰陈硕郑雪薇
Owner UNIV OF SCI & TECH BEIJING
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com