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Fluorescent cell automatic counting method, device, equipment and medium

An automatic counting and cell technology, applied in computing, image data processing, instruments, etc., can solve problems such as large errors and low efficiency of manual cell counting, and achieve the effect of improving accuracy, protecting edge detail information, and shortening manual counting time.

Active Publication Date: 2020-08-25
HUAQIAO UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide an automatic fluorescent cell counting method, device, system, equipment and medium, which overcomes the low efficiency and large error of manual cell counting At the same time, it can replace the current expensive automatic hematology counter at a lower cost, so that it can meet the needs of most laboratories in terms of efficiency, accuracy and cost

Method used

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  • Fluorescent cell automatic counting method, device, equipment and medium
  • Fluorescent cell automatic counting method, device, equipment and medium
  • Fluorescent cell automatic counting method, device, equipment and medium

Examples

Experimental program
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Effect test

Embodiment 1

[0043] This embodiment provides a method for automatic counting of fluorescent cells, such as figure 1 shown, including the following steps:

[0044] Step S1, reading the microscopic image of fluorescent cells;

[0045] Step S2, preprocessing the microscopic image of the fluorescent cells, specifically including:

[0046] Step S3, segmenting the coherent fluorescent cells on the microscopic image;

[0047] Step S4, counting the number of fluorescent cells.

[0048] Wherein, the preprocessing of step S2 specifically includes:

[0049] Step S21, Correct the uneven illumination of the microscopic image, reduce the brightness value of the over-illuminated area at the edge of the image, and increase the brightness value of the over-illuminated area in the middle of the image, until it will be in the over-illuminated area at the edge of the image and the over-illuminated area in the middle of the image The fluorescent cells in the stand out;

[0050] Step S22, enhancing the con...

Embodiment 2

[0107] Such as Figure 9 As shown, in this embodiment a kind of fluorescent cell automatic counting device is provided, comprising:

[0108] A reading module for reading microscopic images of fluorescent cells;

[0109] The preprocessing module is used to preprocess the microscopic images of fluorescent cells, specifically including:

[0110] The segmentation module is used to segment the cohesive fluorescent cells on the microscopic image;

[0111] The counting module is used to count the number of fluorescent cells.

[0112] Wherein, the preprocessing module further includes:

[0113] Correction lighting unit, used to correct the uneven illumination of the microscopic image, reduce the brightness value of the over-illuminated area at the edge of the image, and increase the brightness value of the over-illuminated area in the middle of the image, until the over-illuminated area at the edge of the image and the light in the middle of the image Fluorescent cells in overly d...

Embodiment 3

[0171] This embodiment provides an electronic device, such as Figure 10 As shown, it includes a memory, a processor, and a computer program stored in the memory and operable on the processor. When the processor executes the computer program, any implementation manner in Embodiment 1 can be realized.

[0172] Since the electronic device introduced in this embodiment is the device used to implement the method in Embodiment 1 of this application, based on the method described in Embodiment 1 of this application, those skilled in the art can understand the electronic device of this embodiment. Specific implementation methods and various variations thereof, so how the electronic device implements the method in the embodiment of the present application will not be described in detail here. As long as a person skilled in the art implements the equipment used by the method in the embodiment of the present application, it all belongs to the protection scope of the present application....

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Abstract

The invention provides a fluorescent cell automatic counting method and device, equipment and a medium. The method comprises the following steps: S1, reading a microscopic image of fluorescent cells;S2, preprocessing the microscopic image of the fluorescent cell, including correcting uneven illumination of the microscopic image and enhancing the contrast between the cell in the microscopic imageand the background; adopting median filtering to filter microscopic image noise, and reserving detail information of the edge of the image; removing microscopic image noise and background impurities by adopting opening operation, smoothing cell contours, disconnecting cell stenosis connection and removing small protruding parts of cells; s3, segmenting the fluorescent cells adhered to the microscopic image to prevent the adhered cell population from serving as one cell during cell counting; and S4, calculating the number of the fluorescent cells. Requirements of most laboratories can be met from the aspects of efficiency, accuracy and cost.

Description

technical field [0001] The invention relates to the technical field of cell counting in biomedicine, in particular to a fluorescent cell automatic counting method, device, equipment and medium. Background technique [0002] Cell counting is a routine operation in biomedical experiments and plays an important role in basic scientific research. For example, researchers can study the transduction efficiency of recombinant adeno-associated virus (rAAV) through the number of fluorescent HeLa cells; doctors can accurately determine the cancer stage of patients according to the changes in the number of specific immune cells in diseased tissues. and formulate the best treatment plan. At present, cell counting technology is in great demand and has been widely used in the field of biomedicine. However, the counting efficiency and accuracy need to be further improved. [0003] Traditional manual cell counting under a microscope is not only time-consuming, inefficient, and labor-inten...

Claims

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Application Information

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IPC IPC(8): G06T7/00G06T7/11G06T5/00
CPCG06T7/0012G06T7/11G06T2207/10056G06T2207/20032G06T2207/20192G06T2207/30004G06T2207/30242G06T5/90G06T5/70
Inventor 林跃飞柳培忠刁勇杜永兆张建广黎玲庄加福柳垚
Owner HUAQIAO UNIVERSITY
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