Improved method for dyeing immunofluorescence cell

Abstract

The invention discloses an improved immune fluorescyte staining method, comprising 5 steps: fixing / permeating, permeating, surface staining, staining in nucleus, washing and weight dropping; the method of the invention is an improvement to the Foxp3 immune fluorescyte staining method of eBioscience Company, changes a two-step multiple staining method into a one-step multiple staining method, and carries out the staining on the surface of the cell and in the nucleus simultaneously, thus having the advantages of simple and convenient operation, quick staining, saving reagent, and reducing the cost, etc. The research shows that the method of the invention has no remarkable difference with the method of that of the eBioscience Company, can be used for the staining on the surface of the cell and in the nucleus simultaneously and has extremely good application prospect and promotional value.

Description

technical field

[0001] The invention relates to the biological field, in particular to an improved immunofluorescent cell staining method. Background technique

[0002] Regulatory T cells (Treg) are a type of T cells with immunosuppressive effects, which are an important part of the body's maintenance of self-tolerance, and play an important role in immune-related diseases, tumor immunity, and organ transplantation immunity. . At present, the expression level and clinical significance of peripheral blood Treg cells in patients with immune-related diseases, tumors and organ transplantation have become the focus of research. Studies have shown that the increase in the proportion of Treg cells may be one of the causes of immunosuppression in systemic lupus erythematosus, rheumatoid arthritis, lung cancer, gastric cancer, ovarian cancer, nasopharyngeal cancer, and hepatitis B virus infection; Treg cells in patients before and after treatment Quantitative changes can be used as...

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