4results about How to "Quick stain" patented technology

The invention discloses a non-degreasing biological tissue dyeing method and device. The dyeing method of the non-degreasing biological tissue comprises the following steps: a sample fixing step: fixing a biological tissue sample by adopting paraformaldehyde and / or glutaraldehyde; and a dyeing step: carrying out electrophoresis dyeing on the fixed biological tissue sample which is not degreased by using a nucleic acid dye and a beta-amyloid protein small molecule dye. According to the non-degreasing biological tissue dyeing method disclosed by the invention, the non-degreasing biological tissue is directly dyed, so that the damage of degreasing operation to a sample is avoided; moreover, the nucleic acid dye is combined with the beta-amyloid protein small molecule dye, so that the whole brain sample can be quickly and efficiently dyed, and high-resolution fluorescence labeling and three-dimensional space distribution imaging of beta-amyloid protein plaques in the whole brain range can be realized; the method is of great significance in revealing the spatial distribution pattern of beta-amyloid protein and explaining the pathogenesis of Alzheimer's disease.

The application discloses a dyeing machine based on automatic cleaning and dye liquid adding of dye vat movement, and relates to the technical field of dyeing machines for pathological direction cell dyeing. The application comprises a base plate and a slide rack. A fixed groove frame is arranged at the front part of the upper end of the base plate. A high-temperature dewaxing assembly is arranged at the left side of the upper end of the base plate. The high-temperature dewaxing assembly mainly comprises a high-temperature box, a heat preservation cover and an electric linear drive module. An automatic discharging assembly is arranged at the right side of the upper end of the base plate. The automatic discharging assembly mainly comprises a motor drive module, a feeding groove frame and an adapter plate. A dye vat body is arranged at the upper end of the base plate at the rear of the fixed groove frame. An automatic cleaning and dye liquid adding assembly is arranged at the left side of the upper end of the base plate. A three-axis linkage system is fixed to the upper end of the base plate. Through the arrangement of the three-axis linkage system, the high-temperature dewaxing assembly, the dye vat body and the automatic cleaning and dye liquid adding assembly, the dyeing effect and efficiency are improved, and the dye liquid of the dye vat body can be replaced without affecting the dyeing effect.

The invention discloses an immunofluorescence staining method and device for biological tissues. The biological tissue immunofluorescence staining method comprises the steps of tissue fixation, tissue degreasing and immunostaining. The tissue degreasing comprises the step of degreasing the tissue by adopting a small-micelle zwitterionic surfactant; the immunostaining comprises the step of carrying out immunostaining under a low-voltage condition of 10-100V. According to the immunofluorescence staining method, the small-micelle zwitterionic surfactant is combined with low-voltage current for immunostaining, rapid and uniform staining of large-volume tissues such as mouse brains can be realized on the premise of not causing obvious tissue damage or expansion, and a fine structure is clearly displayed.

The utility model belongs to textile greige cloth dyeing device technical field, especially for a kind of greige cloth dyeing device, aiming at protection problem, present and propose following scheme, it includes dyeing shell;The outer surface of one side of dyeing shell is fixed with drying shell;The outer surface of one side of dyeing shell is equipped with inlet;The inner surface of dyeing shell is rotatably installed with one guide roller;The inner surface of dyeing shell one side is rotatably installed with one extruding roller;The inner surface of dyeing shell upper side is rotatably installed with two extruding rollers;The outer surface of one side of drying shell lower side is equipped with discharge gate;The outer surface of one side of drying shell upper side is equipped with vent. Through the setting of two guide rollers and collecting hopper, the dyeing and drying of dyed cloth are realized quickly, and the residual collecting hopper can also be collected, improving the efficiency;Through the setting of fixed block, the filter drying agent can be replaced, improving the efficiency.