15 results about "Membrane probe" patented technology

The invention relates to the technical field of cytobiology and biomedicine detection, and discloses a time sequence fluorescence tracing system based on a coupling detection lipid probe. Comprising an imaging detection module which is used for adding FM lipophilic styrene fluorescent dye in a culture environment, forming a membrane probe to observe the morphological change of a membrane and detect the formation of vesicles, and completing the complete time sequence tracing of the cell endocytosis process by adopting a content dyeing method; the deep learning module is used for carrying out deep learning on the obtained time sequence image, establishing a living cell imaging screening and image analysis system, and identifying protein molecules related to the target external vesicles; carrying out image description on the generation, transportation and fusion processes of the vesicles generated by the proteins in different stages before fusion of the outer vesicles and the cell membranes and after fusion and shearing; and the knock-down operation module is used for exploring the generation mode of the vesicle contents in the early endosome in combination with knockout and knock-down operations of the specific drug compound.

This application provides an iron-based thin-film probe, an in-situ analysis method for soil micro-interface pollutants, a screening method for iron-based materials for soil remediation, and a soil remediation method, belonging to the field of environmental analysis and testing technology. The iron-based thin-film probe, used to study the role of iron-containing mineral micro-interface pollutants in soil, is prepared from an iron sheet through in-situ surface modification; wherein the modified surface formed by the in-situ surface modification is selected from any one of nano-zero-valent iron, iron oxide, and iron sulfide; the iron-based thin-film probe is used to simulate the adsorption, distribution, and redox reactions of iron-containing mineral micro-interface pollutants in soil.

The invention relates to a film probe card and an adjusting method thereof.The film probe card comprises an installation frame, a flexible film, a supporting unit and a plurality of probes fixed to the bottom face of the flexible film, the installation frame comprises an upper-layer supporting ring and a supporting base, the inner ring of the upper-layer supporting ring is provided with a plurality of bosses, the bosses are in threaded connection with adjusting screws, and the supporting base is connected with the flexible film. A first height adjusting device and at least one elastic piece are arranged between the lower portion of the upper-layer supporting ring and the supporting base, the first height adjusting device is provided with a center hole, the tail end of the adjusting screw penetrates through the center hole of the first height adjusting device to abut against the elastic piece, and a second height adjusting device is arranged between a nut of the adjusting screw and the boss. The supporting unit and the flexible film are both located below the elastic piece. According to the thin film probe card, the part management difficulty can be reduced, screw models are reduced, the adaptability of adjusting screws is improved, and the adjusting range can be accurately controlled.

The present invention discloses a water-soluble tetrazine, a tetrazine membrane probe and a preparation method thereof. The water-soluble tetrazine is not only easy to synthesize, but also, after being connected with hydrophobic BCy, makes the tetrazine membrane probe an amphiphilic molecule, capable of being connected to the cell membrane surface for fluorescence imaging. Moreover, the tetrazine group can undergo an in-situ bioorthogonal reaction with a dienophile, thereby changing the hydrophilicity and hydrophobicity of the tetrazine membrane probe, so that the tetrazine membrane probe can stay on the cell membrane surface for a longer time or enter the hydrophobic environment inside the cell membrane more quickly, thereby flexibly adjusting the targeting ability of the tetrazine membrane probe to the cell membrane.

The invention relates to a supporting structure of a thin film probe card and a testing method of the thin film probe card. The supporting structure comprises a frame body, a fixing piece, a plurality of adjusting mechanisms and a plurality of first elastic structures. The frame body is used for contacting the film sheet. The fixing part is used for fixing the frame body on the substrate and limiting the movable direction of the frame body perpendicular to the substrate. The fixing piece is provided with a plurality of through holes. The plurality of adjusting mechanisms are respectively and movably arranged in the plurality of through holes in a close fit mode. The plurality of first elastic structures are arranged in the first space of the frame body, and each first elastic structure is arranged corresponding to one adjusting mechanism. The plurality of adjusting mechanisms are used for adjusting the elastic force of the plurality of first elastic structures.

The present disclosure provides a supporting structure of a membrane probe card. The supporting structure includes a frame body, a fixture, adjustment mechanisms, and first elastic structures. The frame body is configured to be in contact with the membrane. The fixture is configured to fixate the frame body on the substrate, and confine the frame body to be movable along a direction perpendicular to the substrate. The fixture includes through holes. The adjustment mechanisms are movably and tightly disposed in the through holes, respectively. The first elastic structures are disposed in a first space of the frame body, and each of the first elastic structures corresponds in position to one adjustment mechanism. The adjustment mechanisms are configured to adjust elastic forces of the first elastic structures.

The invention relates to the field of chip testing, in particular to a film probe card structure. The film probe card structure comprises a connecting piece, a flexible film, a fixing piece, a conductive piece, a conductive layer and a plurality of probes, the side portion of the flexible thin film is attached to the bottom face of the connecting piece through the fixing piece, the conductive layer is arranged on the top face of the flexible thin film, the conductive piece is installed on the connecting piece and located above the side portion of the flexible thin film, and the side portion of the conductive layer is located between the conductive piece and the flexible thin film. The side part of the conductive layer is in gapless contact and conductive connection with the conductive piece, the plurality of probes are arranged on the bottom surface of the middle part of the flexible film at intervals, and the probes are in conductive connection with the conductive layer. Due to the fact that conductive connection is achieved between the side portion of the conductive layer and the conductive piece in a gapless contact mode, welding spots do not appear between the side portion of the conductive layer and the conductive piece, transient impedance abrupt change caused by the existence of the welding spots cannot happen to electric signals in the transmission process, and reflection and flowing of the electric signals are effectively prevented.

The utility model relates to the technical field of horizontal adjustment, in particular to a horizontal adjustment device for a film probe card. Comprising a supporting seat, a supporting block, a detection table, an observation assembly, supporting frames, an electric sliding table and the like, the supporting seat is connected with the supporting block, the supporting block is connected with the detection table, the rear side of the supporting seat is connected with the observation assembly in a screw mode, and telescopic rods of two electric push rods are connected with the supporting frames. The two supporting frames are connected with electric sliding tables through screws. Movement of the probe body is observed and controlled in real time through the observation assembly, accurate contact of the probe body is ensured, when a semiconductor is locally uneven, the electric push rod is started, when the surface of the semiconductor is sunken downwards, a telescopic rod of the electric push rod moves downwards for adjustment, and when the surface of the semiconductor protrudes upwards, the telescopic rod of the electric push rod moves upwards for adjustment. And parts such as the support frame, the electric sliding table and the detector are driven to move synchronously so as to compensate the unevenness of the surface and ensure that a probe always keeps good contact with a test point.

The invention discloses a component detection system based on a two-dimensional perovskite thin film probe and a use method thereof, and belongs to the technical field of component detection, the component detection system comprises a two-dimensional perovskite thin film probe subsystem, a sample processing subsystem and a PL spectrum analysis subsystem; the two-dimensional perovskite thin film probe subsystem comprises a first sealing cavity and two-dimensional perovskite thin film probe arrays, and the two-dimensional perovskite thin film probe arrays comprise a formamidine probe array, a methylamine probe array and an ammonia probe array; the sample processing subsystem comprises a second sealing cavity and a sample excitation device; the first sealing cavity is communicated with the second sealing cavity through a gas pipe, a sample to be detected is arranged in the second sealing cavity, component gas is released through the sample excitation device, the component gas is detected through the two-dimensional perovskite thin film probe array, and in-situ detection of gas components formamidine, methylamine and ammonia is achieved by comparing PL spectrums before and after detection. Compared with the gas phase mass spectrometry, the method has the advantages of high detection precision, simplicity in operation and low cost, and is suitable for various scenes in the perovskite field.

The present application provides a kind of water conservancy nondestructive test membrane machine of heap body, it is related to heap body detection technical field.The membrane machine includes control mechanism, executing mechanism and drilling assembly, control mechanism includes drilling rig assembly and pump water component, drilling rig assembly includes rack, drilling rig and slide rail;Executing mechanism includes electric contact pressure gauge, feed power head and valve assembly, drilling assembly includes drill rod and membrane probe drill bit, one end of drill rod is connected with the water outlet of pump water mechanism through water pipe communication, one end of drill rod is connected with feed power head, membrane probe drill bit is set to the other end of drill rod, and high-pressure water flow is made through water pipe by mud pump, and finally high-pressure jet is carried out by membrane probe drill bit and is expanded, and drill rod is automatically followed.When membrane probe drill bit touches waterproof membrane, water flow passage on membrane probe drill bit is blocked, and the water pressure in water pipe is increased, and this pressure signal is transmitted to drilling rig by electric contact pressure gauge, and drilling rig controls drill rod to automatically stop following, and the position of underground waterproof membrane is accurately determined.

The application discloses a kind of protein film macromolecular probe construction and application for biological detection, belong to biological detection and information encryption field.The application connects fluorophore with ligand unit with biological orthogonality, synthesizes a series of new fluorescent molecules capable of specific binding with biological macromolecules.Further through optimizing biological orthogonality reaction, small molecule is covalently combined with HaloTAG protein, and protein film macromolecular probe with good response performance is prepared.The protein film probe uses fusion protein instead of antibody unit in ELISA, can realize the rapid, convenient detection of measured object on solid phase interface, can recycle practical sample, solve the problem that probe and measured object are difficult to separate in liquid phase detection system.Compared with small molecule probe, the protein film probe shows higher sensitivity and signal-to-noise ratio to measured object.

This invention relates to a p-Tau262 detection kit and its applications, particularly suitable for the accurate diagnosis of early Alzheimer's disease. The p-Tau262 detection kit comprises: a first antibody, which is a p-Tau262-specific antibody; and a detection reagent, which includes a second antibody or a lipid probe that specifically recognizes exosome membrane structures. The second antibody recognizes different p-Tau262 antigenic epitopes from the first antibody. By employing a combination of p-Tau262-specific antibodies recognizing different epitopes, or in combination with exosome membrane probes, this invention achieves high-sensitivity, high-throughput detection of the target, addressing the industry pain point of insufficient specificity of existing blood biomarkers in comorbid populations.

The invention relates to the technical field of aerosol detection, in particular to a filter membrane winding probe for online measurement of ultra-large flow aerosol. Comprising a shell, a lead chamber is arranged in the shell, and a probe is arranged in the lead chamber; the conveying cylinder is rotationally connected into the shell, a plurality of guide rollers, an air pump, a first sampling shell and an air storage cylinder are arranged in the shell, a sliding plate is connected into the air storage cylinder in a sealed and sliding mode, the air storage cylinder is communicated with the air pump, and the air storage cylinder is communicated with an air guide pipe; the second sampling shell is connected to an electric sliding rail in the shell in a sliding manner, and the gas guide pipe is communicated with the second sampling shell. Filtered gas is stored through the gas storage cylinder, and residual air in the second sampling shell is removed by using the filtered gas stored in the gas storage cylinder after the filter membrane is replaced, so that the air passing through the filter membrane is real-time air of a target area when the device works again subsequently, and the accuracy and reliability of data detection of the device are ensured.

The application discloses a kind of film probe card in the technical field of round crystal test, to solve the problem of different contact force provided by each probe in the multiple parallel distribution film probe in prior art, affect the working effect of device.It includes mutually connected PCB board and film probe head, film probe head includes support body, thin film arranged on the active surface of support body and multiple rows of probes on the first surface of thin film, each row of probes includes row head probe and row tail probe, each row of probes includes first probe and second probe, and thin film is provided with first rigid element and second rigid element below first probe and second probe respectively;The application is suitable for wafer testing, and the first rigid element and the second rigid element respectively play a reinforcing role on the area below the first probe and the second probe, hinder the rotation of the first probe and the second probe, so that the structure deformation below each probe is basically consistent, and the contact force provided by each probe is basically consistent.

The present disclosure relates to compounds of Formula (I) and their methods of use thereof. The compounds of Formula (I) are conjugated oligoelectrolytes and are suitable for use as a membrane probe to label and / or detect cells and / or lipid vesicles and thus in flow cytometry applications. The present disclosure also relates to a flow system for detecting and / or quantifying cells and / or lipid vesicles.