Wright-Giemsa compound staining solution and preparation method thereof

A technology of Wright's dyeing powder and dyeing solution, applied in the field of biological reagents, can solve the problems of complex dyeing solution formulation, long dyeing time, poor dyeing effect, etc., and achieve the effect of good dyeing effect, fast dyeing, and satisfying dyeing requirements.

Inactive Publication Date: 2016-02-03
成都华西海圻医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are still defects in the currently reported Wright-Giemsa composite dye solution, either the dyeing time is long and the dyeing effect is poor; or the composite dye solution has a complicated formula and high cost

Method used

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  • Wright-Giemsa compound staining solution and preparation method thereof
  • Wright-Giemsa compound staining solution and preparation method thereof
  • Wright-Giemsa compound staining solution and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0043] The preparation of embodiment 1 composite dye liquor of the present invention

[0044] 1. Dye solution formula

[0045] Wright's dye powder 15g, Giemsa dye powder 3g / glycerin 33mL / methanol 5000mL.

[0046] 2. Preparation method

[0047] Take 15g of Wright's dye powder, 3g of Giemsa dye powder, and 33mL of glycerin, grind them in a clean mortar for 2 hours, then place them overnight at 37°C for 15.5 hours;

[0048] After taking it out, grind for 15-20 minutes, add 500mL of methanol, grind for a while, let it stand for 15-20 minutes, and suck out the upper dye solution;

[0049] Then add 500mL of methanol to the mortar, continue to grind for 15 minutes, then place it for 15-20 minutes, and suck out the upper layer of dye solution; in this way, share 5000ml of methanol (analytical pure) several times in a row;

[0050] Collect all the obtained upper dye solution in a brown glass bottle, seal it and store it at room temperature. Shake it for 2 minutes every morning and e...

Embodiment 2

[0053] The preparation of embodiment 2 composite dye liquor of the present invention

[0054] 1. Dye solution formula

[0055] Wright powder 17g, Giemsa powder 4g, glycerin 35mL, methanol 5000mL.

[0056] 2. The preparation method and detection index are the same as in Example 1.

[0057] The beneficial effect of the present invention is illustrated below in the mode of test example:

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Abstract

The invention provides a Wright-Giemsa compound staining solution. The Wright-Giemsa compound staining solution is prepared from the following raw materials according to the following proportion: 15 to 17 parts of Wright staining powder in weight, 3 to 4 parts of Giemsa staining powder in weight, 33 to 35 parts of glycerinum in volume and 5000 parts of methanol in volume. The invention further provides a preparation method and application of the compound staining solution. The Wright-Giemsa compound staining solution has the advantages of high staining speed and good staining effect on human sources and animal cells, simple formula, low preparation cost and simplicity in operation during staining and is favorable for popularization.

Description

technical field [0001] The invention relates to a biological reagent, in particular to a Wright-Giemsa composite dye solution and its preparation method and application. Background technique [0002] Cell staining is the most basic technical means in histology, embryology, pathology, scientific research and teaching. Good staining effect is the primary condition for accurate cell morphology observation. Wright's staining solution and Giemsa's staining solution are conventional staining solutions for cell inspection, but the effect of using them alone is not ideal. Among them, Wright's staining solution is better for staining cytoplasmic components, but the coloring effect for cell nuclei is not satisfactory. Poor; Giemsa stain stained the nucleus better, but stained the cytoplasm, cell membrane and neutral granules in it poorly. [0003] In order to overcome the shortcomings of Wright's and Giemsa's dyes, researchers have developed a compound dye formulation containing both...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30
Inventor 刘斌魏新玉赵璐君
Owner 成都华西海圻医药科技有限公司
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