Fungus fluorescent staining solution and preparation method thereof

A fluorescent dyeing and fungal technology, which is applied in the field of biological testing of pathogenic bacteria in medical clinical testing, can solve the problems that the components of the dyeing solution cannot coexist stably for a long time, the stability of the fluorescent whitening agent is poor, and the stability of the fluorescein is affected, so as to achieve good clinical application. Foreground, improve staining effect and staining durability, avoid the effect of cell self-absorption and self-fluorescence interference

Active Publication Date: 2020-06-16
山东源科生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the fungal fluorescent dyes in the prior art have the following disadvantages: (1) the fluorescent whitening agent has poor stability in a strong alkaline environment, and the fluorescein will precipitate and precipitate; (2) the background pressure agent will affect the stability of the fluorescein Sexuality, it will cause a decrease in detection sensitivity, and even the fungus cannot be stained. Therefore, the components of the staining solution cannot coexist stably for a long time, and they need to be prepared and stored separately. It takes two or even three steps to complete the staining. The operation is relatively cumbersome and the staining time is relatively long. Long; (3) The fluorescence effect is quickly quenched after staining, and the sample cannot be preserved

Method used

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  • Fungus fluorescent staining solution and preparation method thereof
  • Fungus fluorescent staining solution and preparation method thereof
  • Fungus fluorescent staining solution and preparation method thereof

Examples

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Effect test

Embodiment 1

[0036] A fungal fluorescent staining liquid, comprising the following components by weight: 0.8 parts of fluorescent whitening agent 28, 0.05 parts of eosin, 0.02 parts of hematoxylin, 10 parts of dimethyl sulfoxide, 0.5 parts of glycerin, and 0.3 parts of propyl gallate , 0.2 part of ascorbic acid, 0.3 part of surfactant, 0.8 part of sodium chloride.

[0037] The preparation method of above-mentioned fungal fluorescent staining liquid, comprises the following steps:

[0038] (1) Weigh dimethyl sulfoxide, glycerin, propyl gallate, ascorbic acid, surfactant and sodium chloride in corresponding weight proportions, add them into deionized water successively, stir to obtain solution A, and set aside;

[0039] (2) Weigh the fluorescent whitening agent 28 of the corresponding weight and add it into deionized water to fully stir and dissolve to obtain solution B for subsequent use;

[0040] (3) Weigh the corresponding weight of eosin and hematoxylin and add it into deionized water t...

Embodiment 2

[0044] A fungal fluorescent staining liquid, comprising the following components by weight: 1.2 parts of fluorescent whitening agent 28, 0.08 part of eosin, 0.05 part of hematoxylin, 15 parts of dimethyl sulfoxide, 1 part of glycerin, and 0.6 part of propyl gallate , 0.5 parts of ascorbic acid, 0.5 parts of surfactant, and 1.0 parts of sodium chloride.

[0045] The preparation method of above-mentioned fungal fluorescent staining liquid, comprises the following steps:

[0046] (1) Weigh dimethyl sulfoxide, glycerin, propyl gallate, ascorbic acid, surfactant and sodium chloride in corresponding weight proportions, add them into deionized water successively, stir to obtain solution A, and set aside;

[0047] (2) Weigh the fluorescent whitening agent 28 of the corresponding weight and add it into deionized water to fully stir and dissolve to obtain solution B for subsequent use;

[0048] (3) Weigh the corresponding weight of eosin and hematoxylin and add it into deionized water ...

Embodiment 3

[0053] A fungal fluorescent staining liquid, comprising the following components by weight: 1.0 parts of fluorescent whitening agent 28, 0.07 parts of eosin, 0.03 parts of hematoxylin, 13 parts of dimethyl sulfoxide, 0.8 parts of glycerin, and 0.5 parts of propyl gallate , 0.4 part of ascorbic acid, 0.4 part of surfactant, 0.9 part of sodium chloride.

[0054] The preparation method of above-mentioned fungal fluorescent staining liquid, comprises the following steps:

[0055] (1) Weigh dimethyl sulfoxide, glycerin, propyl gallate, ascorbic acid, surfactant and sodium chloride in corresponding weight proportions, add them into deionized water successively, stir to obtain solution A, and set aside;

[0056] (2) Weigh the fluorescent whitening agent 28 of the corresponding weight and add it into deionized water to fully stir and dissolve to obtain solution B for subsequent use;

[0057] (3) Weigh the corresponding weight of eosin and hematoxylin and add it into deionized water t...

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Abstract

The invention belongs to the technical field of biological examination of pathogenic bacteria in medical clinical examination, and particularly relates to a fungus fluorescent staining solution and apreparation method thereof. The fungus fluorescent staining solution is prepared from the following components in parts by weight: 0.5 to 1.5 parts of a fluorescent whitening agent, 0.07 to 0.15 partof a background pressure preparation, 10 to 15 parts of a dissolution promoter, 1 to 2.5 parts of an anti-fluorescence decay agent, 0.3 to 0.8 part of a surfactant and 0.5 to 1.5 parts of sodium chloride. The fluorescent whitening agent and the background dye are mixed and prepared; according to the staining solution, the anti-fluorescence decay agent, the surfactant, the dissolution promoter andthe sodium chloride are added, so that the staining effect and the staining durability of the staining solution are remarkably improved, the staining solution can be stably placed for a long time, specimen staining steps are simplified, and the staining solution has the advantages of high staining speed and good effects.

Description

technical field [0001] The invention belongs to the technical field of biological inspection of pathogenic bacteria in medical clinical inspection, and in particular relates to a fungal fluorescent staining solution and a preparation method thereof. Background technique [0002] Microscopic examination is a commonly used clinical method for detecting fungi, which has the advantages of simple steps and fast speed. As a commonly used microscopic examination method, the KOH method utilizes the principle that high concentration of alkali dissolves tissue cells but cannot dissolve the polysaccharides on the fungal cell wall, and distinguishes fungi by observing the morphology of fungi. Although the KOH fungal detection method is quick and easy, because KOH cannot completely dissolve the tissue, the contrast between fungi and tissue cells is not great, and it is very difficult to identify fungi. Only experienced and professionally trained inspectors can distinguish fungi from tiss...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30G01N21/64
CPCG01N1/30G01N21/6428G01N21/6458G01N2001/302Y02A50/30
Inventor 孙波
Owner 山东源科生物科技股份有限公司
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