Method for identifying discharged live zooplanktons in blast water

A technology of zooplankton and ballast water, applied in the field of identification of living zooplankton, can solve the problems of tediousness, poor detection effect, poisonous hazards for users, etc., and achieve the effect of fast dyeing process, obvious effect and low cost

Inactive Publication Date: 2012-08-15
SHANGHAI OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to overcome the drawbacks of the prior art, which usually takes a long time and is cumbersome when detecting the activity of marine plankton, and also has relatively high costs and may cause toxic hazards to users, and its detection effect is also relatively poor. Provide a simple, fast and reliable detection method for defects with unstable results after detection

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 is a detection of living zooplankton in a sample of zooplankton in ballast water (the sample is a simulated ship ballast water sample provided by Shanghai Jiazhou Environmental Protection Electromechanical Equipment Co., Ltd.).

[0030] 1 reagent

[0031] 1.1(1) Prepare Janus Green B staining solution:

[0032] Sodium chloride 1.00g

[0033] Potassium chloride 0.25g

[0034] Distilled water 100ml

[0035] Dissolve sodium chloride and potassium chloride in a small amount of distilled water respectively, mix and dilute to 100ml with distilled water.

[0036] (2) 1.5% Janus Green B solution

[0037] Weigh 1.5g of Janus Green B and dissolve it in 100ml of buffer solution, heat it slightly at 40°C-45°C to dissolve it quickly, and filter it with filter paper (qualitative filter paper) to obtain 1.5% Janus Green B solution (generally After preparation, keep it away from light to maintain its full oxidation capacity, and the validity period is 20 days).

[0038]...

Embodiment 2

[0045] The reagents and methods of this embodiment are substantially the same as in Example 1, except that in the staining step 2.1, 1.0% zooplankton staining solution is added dropwise to each group of water samples per liter to be 1.0ml (1g potassium iodide, 0.25g iodine Add 100ml of 1.5% Janus Green B solution). The detection results of zooplankton samples in ballast water (the samples were simulated ship ballast water samples provided by Shanghai Jiazhou Environmental Protection Electromechanical Equipment Co., Ltd.) found that the body parts of live zooplankton were lighter blue after staining and fixing, and dead zooplankton After dyeing and fixation, the whole body is dark blue.

Embodiment 3

[0047] The reagents and methods of this embodiment are substantially the same as in Example 1, except that the 2.0% zooplankton staining solution added dropwise to each group of water samples per liter in the staining step 2.1 is 2.0ml (2g potassium iodide, 0.5g Iodine was added to 100ml of 1.5% Janus Green B solution). The detection results of zooplankton samples in ballast water (the samples were simulated ship ballast water samples provided by Shanghai Jiazhou Environmental Protection Electromechanical Equipment Co., Ltd.) found that the body part of the live zooplankton was darker blue after staining and fixation, and the dead zooplankton stained After fixation, the whole body is dark blue.

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Abstract

The invention provides a method for identifying discharged lived zooplanktons in blast water. The method includes steps: (1) dripping from 1.0 to 2.0ml of 1-2% zooplankton staining solution into every 60ml of acquired zooplankton water sample, and realizing staining; (2) further dripping formalin liquid into the water sample to fix the sample; and (3) realizing microscopic examination for the sample after the sample is uniformly mixed, and counting. Compared with the prior art, an integral staining process of the method is speedy and is simplified, cost is low, the effect is obvious, and whether the blast water contains the lived zooplanktons or not can be quite easily identified.

Description

technical field [0001] The invention relates to a method for identifying living zooplankton in water samples, in particular, the invention relates to a method for identifying living zooplankton discharged from ballast water. Background technique [0002] Ballast water refers to the water and its suspended matter added to the ship to control the trim, heel, draft, stability or stress of the ship. When the ship arrives at the shore, in order to free up the tonnage, the ballast water must be discharged into the sea area of ​​the landed country. According to relevant data, the ballast water carried by ships around the world is about 12 billion tons per year. Ballast water and ballast tank sediments contain a large number of organisms and microorganisms, including bacteria, algae, protozoa, plankton and others. biology. On average, there are more than 100 million zooplankton per cubic meter of ballast water. There are about 7,000 species of marine organisms that exist in the ba...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/84G01N1/30
Inventor 薛俊增刘艳吴惠仙
Owner SHANGHAI OCEAN UNIV
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