Anti-attenuation fungal fluorescent dyeing liquid and preparation method thereof

Abstract

The invention discloses an anti-attenuation fungal fluorescent dyeing liquid and a preparation method thereof. Wherein the components of the anti-attenuation fungal fluorescent dyeing liquid include:a fluorescent agent, a solubilizing agent, a penetrating agent, a background dye, an anti-fluorescent attenuation agent, a stabilizer, and deionized water. According to the anti-attenuation fungal fluorescent dyeing liquid and the preparation method thereof, each component is stabilized in a solution system, and filtered by a cellulose membrane, all kinds of minute impurities are effectively removed, thereby having an effect of long-term storage without crystallization and having the advantages that the fungal fluorescence mark and the background dyeing are synchronously carried out in a few seconds; the anti-attenuation fungal fluorescent dyeing liquid does not need to be operated in a dark room, the performance is relatively stable, and has the characteristics of convenient operation, short dyeing time, long attenuation time, high efficiency, comprehensive use range, high observation clarity, good specificity and accurate detection result, and has a good clinical application prospect.

Description

technical field The invention belongs to the field of fluorescent dyeing liquid, and in particular relates to an anti-attenuation fungal fluorescent dyeing liquid and a preparation method thereof. Background technique Fungi are a kind of eukaryotic organisms. Tens of thousands of fungi have been discovered, of which more than 270 are pathogenic to humans. Nowadays, with the accelerated pace of life, the number of people with low immunity is increasing year by year, so that the diseases caused by fungal infections are on the rise. Fungi have a solid cell wall and a true nucleus, live in a parasitic or saprophytic manner, typically have both sexual and asexual reproduction, and produce various forms of spores. Fungi can easily cause fungal skin diseases, also known as skin mycosis, which refers to a large class of infectious diseases caused by pathogenic fungi on human skin, mucous membranes, hair, nails and other appendages, and belongs to superficial mycoses. Common as: t...

AI Technical Summary

Problems solved by technology

Although the culture method has high accuracy of detection results, due to the slow growth of fungi, it takes days or even weeks to obtain results due to the slow growth of fungi, which often leads to untimely diagnosis and delayed treatment

Although immunological detection has good specificity, it can only be tested for antibodies against deep fungal infections. Second, for patients who have developed antibodies for a long time during the recovery from infection, there is a certain false positive rate in this group of people, so the application of this method is limited

However, there are still defects such as insufficient contrast between fungus and background, poor staining effect, rapid quenching of fluorescence effect after staining, interference of crystalline substances and other fluorescent impurities under the microscope, and obvious crystallization of reagents after long-term storage.

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