Method for rapid Wright-giemsa stain

Abstract

The invention discloses a method for rapid Wright-giemsa stain. The method includes: preparing Wright-giemsa working fluid, PBS (polybutylene succinate), a stain-fixative, appropriate ionized water and experimental apparatuses; dropping a solution to be tested on a glass slide with a pipette, spreading the solution evenly on a smear and drying in the shade naturally; fixing the glass slide for 1-3minutes with the stain-fixative and placing the glass slide on a stain frame; dropwise adding the Wright-giemsa working fluid to the smear to have the same evenly covered, wherein the operation environment is controlled at room temperature, and time is controlled at 10-15 minutes; after stain is completed, placing the smear in the PBS, and shaking the smear slightly for standby for 5-10 minutes;taking out the standby smear and performing rinsing from one end of the smear with deionized water; performing microscopic examination after drying. Through combined utilization of giemsa stain and Wright-giemsa stain, good stain effect can be obtained, stain time can be shortened, rapid stain and microscopic examination can be performed, and clinical diagnosis is assisted.

Description

technical field [0001] The invention relates to the technical field of Wright's Giemsa staining, in particular to a method for quickly performing Wright's Giemsa staining. Background technique [0002] Wright's staining is the most commonly used and simplest staining method at present. The acid eosin and basic methylene blue in Wright's staining solution are mixed and chemically reacted to become neutral eosinized methylene blue. It is oxidized and contains azure. These three dyes combine with NH3+ and COO- in the nucleus and plasma respectively to color the nucleus and cytoplasm. Since it is a neutral dye and there is a buffer to adjust the pH, so after the cells are infected, The colors such as blue and red are relatively moderate, and the chromatin, cytoplasm and granules in them are more clearly colored. Giemsa staining solution is composed of azure and eosin. The staining principle and result are basically the same as Wright's staining method; eosinophilic granules It ...

AI Technical Summary

Problems solved by technology

[0002] Wright's staining is the most commonly used and simplest staining method at present. The acid eosin and basic methylene blue in Wright's staining solution are mixed and chemically reacted to become neutral eosinized methylene blue. It is oxidized and contains azure. These three dyes combine with NH3+ and COO- in the nucleus and plasma respectively to color the nucleus and cytoplasm. Since it is a neutral dye and there is a buffer to adjust the pH, so after the cells are infected, The colors such as blue and red are relatively moderate, and the chromatin, cytoplasm and granules in them are more clearly colored. Giemsa staining solution is composed of azure and eosin. The staining principle and result are basically the same as Wright's staining method; eosinophilic granules It is a basic protein, combined with acid dye eosin, dyed pink, called eosinophilic substance; cell nucleus protein and lymphocyte cytoplasm are acidic, combined with basic dye methylene blue or azure, dyed purple blue, called Basophilic substances; neutral particles are in an isoelectric state and can be combined with eosin and methylene blue to dye lavender, which is called neutral substances; in order to take into account the length of the two, compound dyeing method can be used, but the traditional dyeing process takes longer Long, during the staining process, it is cumbersome to mix the dye solution A and the dye solution B on the slide by blowing and beating. Therefore, we propose a rapid Wright Giemsa staining method