486 results about "Methylene blue" patented technology

The invention relates to the technical field of adsorbent preparation, in particular to an ammonia modified porous carbon adsorbent and a preparation method thereof. The preparation method of the ammonia modified porous carbon adsorbent comprises the following steps: carrying out hydrothermal acid leaching on gasified fine residues and an acid solution to obtain acid leaching residues; stirring the acid leaching residues in an alkali solution, washing and drying to obtain a porous carbon material; and dipping the porous carbon material in a tetraethylenepentamine solution, and then drying to obtain the ammonia modified porous carbon adsorbent. The ammonia modified porous carbon adsorbent is successfully constructed by taking a porous carbon material derived from coal gasification fine slag as a substrate and further adopting a surface ammonia functionalization modification strategy. The preparation method is simple, the raw materials are easy to obtain, and the prepared ammonia modified porous carbon adsorbent has a good adsorption effect on methylene blue and hexavalent chromium. According to the method, high-added-value resource utilization of the coal gasification solid waste is achieved, and a solution is provided for treatment of the dye wastewater and the heavy metal wastewater.

The invention discloses a preparation method of a sandwich structure immunosensor based on surface enhanced Raman scattering and application of the sandwich structure immunosensor to detection of an anti-phospholipase A2 receptor antibody, and is characterized by comprising the following steps: sputtering silver ions on a cotton swab / ZIF-67 prepared by an in-situ growth method by adopting an ion sputtering method to obtain a cotton swab / ZIF-67 / Ag NPs composite material; the preparation method comprises the following steps: preparing an antigen solution, then adding the antigen solution into the antigen solution to obtain an immune substrate, adding a methylene blue aqueous solution into a silver nanoparticle solution for reaction, then connecting an anti-human PLA2R IgG antibody to obtain an immune probe, and finally dropwise adding a phosphate buffer solution containing an antibody to be detected onto the immune substrate, and dropwise adding the immune probe solution to the immune substrate adsorbed with the antibody to be detected to obtain the sandwich structure immunosensor based on surface enhanced Raman scattering. The method has the advantages of high sensitivity, good selectivity and strong stability.

The invention relates to a universal method for co-adsorbing perrhenate or pertechnetate by using cationic dye modified activated carbon, and belongs to the technical field of water treatment. In the system provided by the invention, the cationic dye modified activated carbon is used as an adsorbent of perrhenate or pertechnetate, and the cationic dye / activated carbon composite material realizes efficient removal of pollutants by co-adsorbing the perrhenate or pertechnetate in sewage. According to the invention, 12 activated carbon is used as a porous carrier, and after five different cationic dyes are respectively adsorbed, perrhenate radicals can be effectively adsorbed. Particularly, after methylene blue dye is adsorbed, perrhenic acid radicals can be quickly adsorbed within an extremely short time, and the adsorbent has the advantages of large adsorption capacity, good pH stability, high selectivity, good radiation resistance, low cost and the like, and can be applied to actual environmental sewage.

The multi-channel electrochemical micro-fluidic chip detection platform provided by the invention realizes rapid, high-throughput, low-cost, high-sensitivity and specific detection on genital tract pathogens. Specifically, when a target exists, a target recognition probe Cas12a-crRNA compound specifically recognizes a target and forms a Cas12a-crRNA-Target triad, the trans-cleavage activity is activated, an activated Cas12a system cuts a G1 / G2 single-chain region in the signal amplification probe, then encapsulated methylene blue is released, the methylene blue further promotes a methyl bromide redox reaction, signal transduction is promoted, and the target recognition probe Cas12a-crRNA compound can be used for recognizing the target. Therefore, high-sensitivity and high-specificity quantitative detection of pathogens is realized. On the basis, through a multi-channel micro-fluidic chip, high-sensitivity detection of multiple targets can be realized at the same time by placing different crRNA sequences in reaction tanks respectively. In the embodiment of the invention, the multi-target ultra-sensitive detection of CT, CA, TV and NG is successfully realized, and the detection limit is as low as 0.8 fM.

The invention belongs to the field of graphene textile wastewater treatment, and particularly relates to a graphene oxide-based zwitterionic composite filtering film as well as preparation and application thereof. The SBMA is modified to the GO surface through graft polymerization to form a physical intercalation composite structure, so that the hydrophilicity, interlayer spacing and antifouling property of the membrane are remarkably improved. The retention rate of the composite membrane on methylene blue exceeds 99%, the salt separation factor reaches 180.75, and the flux recovery rate (FRR) is kept above 80% after circulation. The membrane preparation process is green and environment-friendly, is suitable for high-efficiency separation of dye and salt in textile wastewater, and has a wide application prospect.

The invention relates to the technical field of adsorbent preparation and methylene blue adsorption, in particular to application of an ammonia modified porous carbon adsorbent in methylene blue adsorption. An ammonia modified porous carbon adsorbent is added into a water sample containing methylene blue for adsorption, so that the methylene blue in the water sample is removed; the preparation method of the ammonia modified porous carbon adsorbent comprises the following steps: carrying out hydrothermal acid leaching on gasified fine residues and an acid solution to obtain acid leaching residues; stirring the acid leaching residues in an alkali solution, washing and drying to obtain a porous carbon material; and dipping the porous carbon material in a tetraethylenepentamine solution, and then drying to obtain the ammonia modified porous carbon adsorbent. The ammonia modified porous carbon adsorbent is successfully constructed by taking a porous carbon material derived from coal gasification fine slag as a substrate and further adopting a surface ammonia functionalization modification strategy. The ammonia modified porous carbon adsorbent has a good adsorption effect on methylene blue.

The invention belongs to the technical field of medical instruments, and discloses a digestive endoscopy diseased region accurate positioning and marking device which comprises a controller, an endoscope hose is fixedly connected to the bottom of the controller, and an instrument insertion opening is formed in the right side of the controller. Through the arrangement of the sleeve, the dye tube, the pressurization air bag and the baffle, after an operator observes a diseased region through the endoscope hose, the operator only needs to align the endoscope hose to the diseased region, and by pressing the pressurization air bag, air in the pressurization air bag enters the dye tube through the inner hose; the baffle can be pushed to rotate outwards under the action of air pressure, meanwhile, methylene blue in the dye tube is sprayed out under the action of air flow, a diseased region is marked, the methylene blue can be absorbed by normal mucous membrane cells, and diseased tissue shows dyeing difference due to abnormal cell function and poor absorption capacity, so that the diseased region is marked. Therefore, the effect of accurate positioning is achieved.

The invention discloses a preparation method of a halloysite nanotube adsorption material with sulfonic groups polymerized on the surface, and belongs to the technical field of wastewater treatment materials, the preparation method comprises the following steps: S1, adding 5.0 g of halloysite nanotubes and 5 mL of gamma-methacryloxypropyltrimethoxysilane into 100 mL of toluene; s2, in a magnetic stirrer, condensing and refluxing at 120 DEG C for 10 hours, cooling, filtering, washing with toluene and ethanol for multiple times to remove unreacted KF570, and drying a product in a vacuum drying oven at 60 DEG C for 8 hours to obtain a KH570 modified halloysite nanotube; s3, weighing 1.0 g of HNTs-KH570 as a raw material, dispersing the HNTs-KH570 in 80 mL of distilled water, adding 5.0 g of 2-acrylamide-2-methylpropanesulfonic acid, performing uniform ultrasonic dispersion, and continuously stirring at the temperature of 70 DEG C; and S4, adding 0.15 g of ammonium persulfate into 20 mL of distilled water, and fully stirring to completely dissolve the ammonium persulfate. The adsorption performance of the halloysite nanotube to methylene blue is remarkably improved through sulfonic acid group modification, and the adsorption capacity is improved by 270%.

The invention discloses an electrochemical urine biopsy system for cathepsin B analysis and a detection method of the electrochemical urine biopsy system. The electrochemical urine biopsy system comprises gold nanoparticles AuNPs, a peptide-peptide nucleic acid PNA series probe and an electrochemical sensor. The peptide-peptide nucleic acid tandem probe is combined with gold nanoparticles through a mercaptan modified sequence to form a spherical nanostructure with the diameter of 15 nm, a polypeptide substrate sequence serves as a specific reaction substrate of cathepsin B, and PNA provides stability and a signal shielding function. The electrochemical sensor is based on a cutting enzyme-assisted DNAwalker mechanism, signal amplification is started through methylene blue MB release, and high-sensitivity detection of the released PNA probe is achieved. The system provided by the invention has the characteristics of high specificity, high sensitivity and rapid response, can realize quantitative analysis of cathepsin B through noninvasive urine biopsy, and provides a new technical means for early diagnosis and treatment monitoring of tumors.

Methylthioninium salt-containing compositions can include methylthioninium chloride (Methylene Blue) in combination with additional compounds that work cooperatively and / or synergistically to treat various conditions. In one example, the methylthioninium salt-containing composition can include methylthioninium chloride, caffeine, and L-theanine. The methylthioninium chloride and the caffeine can be present at a weight ratio from about 1:75 to about 1:4. the methylthioninium chloride and the L-theanine can be present at a weight ratio from about 1 :200 to about 1:10, and the caffeine and the L- theanine can be present at a weight ratio from about 1 :5 to about 1:1.

The invention relates to a method for preparing a magnetic Fe3O4 / Cu / nitrogen-doped carbon composite material by a one-step method and an application of the magnetic Fe3O4 / Cu / nitrogen-doped carbon composite material, and aims to solve the problems of poor catalytic activity, complex separation and recovery and complex synthesis method of the existing PMS activated catalytic material. According to the method, melamine is selected as a carbon source and a nitrogen source, ferric nitrate and copper sulfate are selected as an iron source and a copper source respectively, and the magnetic Fe3O4 / Cu / nitrogen-doped carbon composite material is synthesized in one step by adopting a high-temperature pyrolysis method. The adsorbent not only has excellent adsorption performance on dye-methylene blue, but also can realize high-efficiency oxidative degradation. The magnetic Fe3O4 / Cu / nitrogen-doped carbon composite material prepared by the invention not only has excellent catalytic performance, but also is easy to magnetically separate and recycle, and is suitable for efficient treatment of dye wastewater. The method is applied to the field of catalytic degradation of wastewater.

The invention discloses a preparation method of a modified adsorption material, and belongs to the field of water treatment. The method comprises the following steps: selecting analytically pure hydrochloric acid with the mass fraction of 37%, preparing a 10% hydrochloric acid solution, adding activated carbon into the hydrochloric acid solution according to the mass-volume ratio of 1g: 10mL, and shaking until the hydrochloric acid is fully contacted and reacted with the activated carbon; after the reaction is finished, washing with a large amount of deionized water until the pH value is close to 7, and then putting the washed activated carbon into a drying oven to obtain activated carbon pretreated by hydrochloric acid; through hydrochloric acid pretreatment, etching and chambering, a pore structure is optimized, functional groups such as nitryl and the like are directionally introduced through potassium nitrate high-temperature activation, a chambering-functionalization synergistic effect is formed, the specific surface area of the activated carbon is increased, the mesopore proportion is increased, the nitrogen element content reaches 2.78%, the adsorption capacity of pollutants such as methylene blue is increased, meanwhile, the raw material cost is low, and the environment friendliness is good. The problems that existing activated carbon is insufficient in pore structure regulation and control, lack of functional groups, low in adsorption efficiency, degraded in regeneration performance and the like are effectively solved.

The invention discloses an IEF-11 / WO3-xS type heterojunction material with full spectrum response as well as a preparation method and application of the IEF-11 / WO3-xS type heterojunction material. The heterojunction is prepared by taking squaric acid, tetrabutyl titanate and WO3-x particles as raw materials, wherein the value range of x in WO3-x is 0 < = xlt; 1. The heterojunction material creatively realizes full-spectrum response from ultraviolet to near infrared through S-type energy band structural design, the separation efficiency of photo-induced electron-hole pairs reaches 2.47 times (compared with WO3-x) and 1.57 times (compared with IEF-11) due to a unique carrier transport mechanism, and strong redox sites are synchronously reserved, so that the material can be applied to the field of photoelectron-hole separation. And compared with a single-component material, the photocatalytic performance is improved by 3.74 times (compared with WO3-x) and 1.41 times (compared with IEF-11). Experiments show that under full-spectrum irradiation, the inactivation rate of the material to escherichia coli can reach 99%, the 120-minute degradation rate of the material to tetracycline (TC) reaches 86%, the material shows broad-spectrum degradation capacity to typical organic pollutants such as chlortetracycline (CTC), rhodamine B (RhB) and methylene blue (MB) in a visible region, and the 120-minute degradation efficiency reaches 77%, 53% and 95% respectively. The heterojunction material is prepared through a simple hydrothermal method, and has important application value in the fields of environmental governance, photocatalytic disinfection and the like.

The present invention belongs to the technical field of rapid detection of drug sensitivity for bacteria. Disclosed are an enrichment culture medium, and a preparation method therefor and the use thereof. The enrichment culture medium comprises the following raw materials in parts by mass: 15.0-20.0 parts of tryptone, 3.5-5.5 parts of peptone, 1.5-3.5 parts of glucose, 5.0-6.5 parts of yeast extract, 2.0-2.5 parts of dipotassium phosphate, 2.0-3.5 parts of disodium hydrogen phosphate, 1.0-1.5 parts of monopotassium phosphate, 2.5-7.5 parts of sodium chloride, 12.0-18.0 parts of proteose peptone, 15.0-22.0 parts of beef heart infusion powder, 8.0-12.0 parts of soya peptone, 1.0-5.0 parts of 0.1% nicotinamide adenine dinucleotide (NAD), 30-100 parts of newborn calf serum inactivated at 56°C for 30 min, 2.0-4.0 parts of mannitol, 3.0-5.0 parts of sodium pyruvate, 0.23-0.45 parts of cycloheximide, 0.8-2.0 parts of 0.05% bromothymol blue, 2.5-4.5 parts of 0.1% methylene blue, and 1000 parts of distilled water, with the pH value of 6.8-7.5. The enrichment culture medium is used to screen effective therapeutic drugs for bacterial diseases in poultry, and is convenient to use and has a single enrichment effect superior to that of respective selective enrichment broths of target bacteria. Therefore, the culture medium can realize rapid proliferation of common pathogenic bacteria in poultry.

The invention discloses a polyacid-based metal viologen framework crystalline material as well as a preparation method and application thereof, and belongs to the technical field of heterogeneous catalytic materials. The crystalline material is synthesized in one step through a hydrothermal method, the molecular formula is Cu2 (H2O) 2 (L) 2 [PW12O40] [OH]. 2H2O, the crystalline material belongs to a monoclinic system, the space group is P21, the cell parameters are alpha = 90 degrees, beta = 114.208 (2) degrees and gamma = 90 degrees, and the structure of the crystalline material is determined through X-ray diffraction. According to the material, accurate compounding of polyacid clusters and metal viologen on the molecular level is achieved, an effective electron transmission channel is constructed, separation of photon-generated carriers is remarkably promoted, and excellent photocatalytic activity is shown. At the ambient temperature, the crystalline material can be used as a catalyst to efficiently degrade dye pollutants (such as methylene blue and rhodamine B) in a water body, the degradation rate within 60 minutes exceeds 90%, and the crystalline material has good cycle stability and structural durability.

The invention discloses an indium-based metal-organic framework material based on a pyridine hexacarboxylic acid ligand as well as a preparation method and application of the indium-based metal-organic framework material, the chemical formula is {[NH2Me2] 2 [In4 (mu2-O) (H6L) 2 (H2O) 2]. 3DMF. 10H2O} n, and H6L is an organic ligand 4, 4 ', 4' '-(pyridine-2, 4, 6-triyl) triisophthalic acid. The material has excellent thermal stability and chemical stability, and due to the frame charge characteristic and the excellent pore structure of the material, the metal organic frame material shows efficient dye selective adsorption and separation performance, can selectively adsorb cationic dye methylene blue MB and repel anionic dye methyl orange MO at the same time. In conclusion, a new design thought is developed for the novel indium-based metal organic framework material based on the pyridine hexacarboxylic acid ligand, and meanwhile, a new candidate is provided for developing a high-efficiency dye wastewater purification material.

The invention discloses a coated carbon material as well as a preparation method and application thereof, and belongs to the technical field of recycling of coated carbon materials. The preparation method comprises the following steps: adding lithium iron phosphate powder into an acid solution, heating to 30-80 DEG C, adding an oxidant or introducing oxygen, reacting at the same temperature for 3-24 hours, carrying out suction filtration, and drying an obtained filter cake to obtain the coated carbon material. The preparation method disclosed by the invention is simple and convenient in process and low in energy consumption, and the prepared coated carbon material can be used for preparing an organic matter adsorbent and an organic matter degradation catalyst and shows excellent performance in adsorption of methylene blue in wastewater and catalytic degradation of sulfonamide antibiotics; the carbon material in the lithium iron phosphate battery is recycled, waste is treated by waste, and the current social environment problem can be effectively solved.

The invention discloses a kit for detecting an A beta oligomer. The kit comprises a nucleic acid aptamer-activator modified streptavidin magnetic bead, a methylene blue nucleic acid signal probe modified streptavidin magnetic bead, a Cas12a-crRNA compound and a synthetic gold nanocluster probe modified glassy carbon electrode. According to the invention, the beta-CD-AuNCs probe with high quantum yield is used as an electrochemiluminescence material, methylene blue is used as an electrochemiluminescence quencher, and through combination with CRISPR / Cas technology, signal change of an ECL reaction system is effectively regulated and controlled, and AbetaO detection is realized. The method has the advantages of strong detection specificity, good selectivity, high sensitivity and strong anti-interference capability on AbetaO, and has good market value.

This invention discloses an amplification-free electrochemical biosensor and method based on CRISPR-Cas13a. The sensor includes an electrochemical biosensor substrate, a dual-channel RNA reporter molecule, a CRISPR-Cas13a reaction system, and a dual-channel differential signal processing module. The working electrode surface is immobilized with a stem-loop structured first reporter molecule and a linear second reporter molecule loaded with gold nanoparticles, labeled with methylene blue and ferrocene signal molecules respectively, and immobilized with a ferrocene-specific capture probe. When target RNA is present, Cas13a is activated and non-specifically cleaves the reporter molecule, reducing the methylene blue signal and enriching and enhancing the ferrocene signal. Background self-calibration is achieved through dual-channel differential ratio calculation. This invention integrates signal attenuation and signal enhancement modes, offering advantages such as nucleic acid amplification-free operation, fast detection speed, high sensitivity, and strong anti-interference capability.

The invention relates to the technical field of dye separation, and discloses a loose nanofiltration membrane and a preparation method and application thereof, the loose nanofiltration membrane comprises a porous support membrane, and a polyesteramide separation layer formed on the porous support membrane through one-step interfacial polymerization of nucleoside monomers and multi-acyl chloride monomers. The nucleoside monomer is one or more than two of adenosine, guanosine and cytidine nucleoside. The nucleoside monomer with a long-chain structure, a branched-chain structure, a rigid twisted structure and a plurality of hydrophilic reaction sites is selected as an interfacial polymerization water-phase monomer, so that the loose nanofiltration membrane is endowed with excellent permeation and separation performance. The membrane has a high rejection rate on dye molecules such as Congo red and methyl blue, also has high water permeability and extremely high dye / inorganic salt separation selectivity, and shows good application potential in the aspects of dye production and purification and dye wastewater recovery.

The application discloses a high-efficiency degradation of dyes and antibiotics high-entropy spinel ferrite catalyst and a preparation method and application thereof. 1 / 2 Zn 1 / 2 Fe 2 / 3Co 2 / 3 Ni 2 / 3 O4(HEO-FNCCZ) high-entropy spinel ferrite catalyst, the photocatalytic degradation performance of common dyes and antibiotics is evaluated by using simulated visible light photocatalysis, and the reaction mechanism of the photocatalytic degradation of methylene blue with the best effect is studied. The catalytic effect of the spinel ferrite is utilized to effectively promote the photocatalytic reaction, so that the environment-friendly pollutant degradation is realized. The method has the characteristics of simple production process, no introduction of various organic toxic and harmful substances, non-toxic and harmless in use, catalyst recovery efficiency more than 95%, and no secondary pollution. The preparation method only involves processes such as dissolving, calcining and crushing, and is extremely suitable for industrialized batch production.

The invention discloses a black phosphorus-methylene blue nano composition for improving blood brain permeability and a synthesis method thereof. The synthesis method comprises the following steps: step 1, mixing black phosphorus nanosheets with polyethylene glycol for reaction; step 2, carrying out centrifugal cleaning on the reaction liquid in the step 1, and collecting a lower-layer reactant; step 3, adding aminated superparamagnetic nanoparticles and methylene blue into the lower reactant obtained in the step 2 for reaction; and 4, carrying out centrifugal cleaning on the reaction liquid obtained in the step 3, and collecting a lower-layer reactant. The invention also discloses the black phosphorus-methylene blue nano composition synthesized by the synthesis method, the drug loading rate of methylene blue in the composition exceeds 60%, and the composition has good biological safety, is non-toxic to cells and mice, is very simple and convenient in preparation process, and has a wide application prospect in the aspect of improving the blood-brain barrier permeability.

The invention belongs to the technical field of preparation and application of metal organic framework adsorption materials, and particularly relates to a Ti-MOF-based nanometer material, a preparation method thereof and application of the Ti-MOF-based nanometer material in methylene blue dye adsorption, and a hierarchical pore Ti-MOF-based nanometer methylene blue adsorbent rich in oxygen vacancies is constructed by means of a temperature regulation and control ligand pyrolysis strategy. Adsorption performance tests show that the adsorbent has high adsorption capacity and high adsorption rate on methylene blue, due to the fact that hierarchical pores in the adsorbent promote diffusion and mass transfer of methylene blue, and oxygen vacancies enhance electronegativity of the surface of the adsorbent and provide more adsorption active sites.

A flexible near-infrared II region photodetector based on two-dimensional material nanoribbons relates to the technical field of optoelectronic devices, and solves the problems of poor flexibility and low responsivity of existing near-infrared II region photodetectors. It includes: a flexible substrate; an inorganic nanoribbon array disposed on the upper surface of the flexible substrate; a metal electrode disposed on the upper surface of the inorganic nanoribbon array; an organic modification layer connecting the inorganic nanoribbon array, and the near-infrared II region spectral absorption rate of the organic modification layer is not lower than that of methylene blue, porphyrin compounds or indocyanine green in the near-infrared II region. The preparation method is: preparing an inorganic nanoribbon array on the flexible substrate; preparing an organic modification layer on the inorganic nanoribbon array. The present invention improves the optoelectronic conversion efficiency of the device through the energy band distribution and coupling between two-dimensional organic and inorganic materials; improves the responsivity of the device in the near-infrared II region; can be attached to the surface of a living body for real-time detection of a moving living body.

The invention relates to an application of a nanosphere fluorescent probe combined CRISPR (clustered regularly interspaced short palindromic repeats) in detection of exosomes, which comprises the following steps: inserting DNA nanospheres as a carrier of fluorescent signal molecule methylene blue into a cholesterol probe on an exosome membrane to start a strand displacement reaction, activating trans-cleavage activity of Cas12a by a reaction product to split the DNA nanospheres wrapped with methylene blue, further, qualitative and quantitative analysis of the exosome is realized through fluorescence signal detection of methylene blue. Compared with a common CRISPR / Cas-based biosensing platform, the nanosphere fluorescent probe and the CRISPR are combined, so that high-sensitivity detection of the exosome can be realized, and the application scene of the nucleic acid nanoprobe and the CRISPR-Cas system in fluorescent biosensing is widened.

The invention discloses a printing and dyeing wastewater nanofiltration membrane anti-pollution-separation performance evaluation method, and belongs to the field of membrane separation technology performance detection.The evaluation method comprises the steps that a to-be-detected nanofiltration membrane is designed for staged pollution experiments, actual printing and dyeing wastewater is filtered, and the nanofiltration membrane for continuously filtering the printing and dyeing wastewater is subjected to staged cleaning regeneration; and testing data such as flux, methylene blue rejection rate and NaCl rejection rate in the experiment process, calculating a flux comprehensive attenuation rate, a graded recovery rate, a separation performance stability coefficient, a charge stability coefficient and a hydrophilic promotion rate, and evaluating the anti-pollution-separation performance grade of the to-be-tested nanofiltration membrane according to calculation results. The technical defects that parameters of a traditional evaluation system are disjointed and dimensions are one-sided are overcome, and the evaluation method can directly guide modification optimization and engineering type selection of the nanofiltration membrane for printing and dyeing wastewater treatment.

This invention discloses a molecularly switched adsorbent for adsorbing triphenylmethane dyes and its application, belonging to the field of wastewater treatment technology. The molecularly switched adsorbent proposed in this invention can rapidly remove triphenylmethane dyes such as crystal violet, malachite green, and methylene blue dyes from water; it can remove tens of ppm concentrations of crystal violet, malachite green, and methylene blue dyes from water in a very short time (90 min), with a removal rate of ≥92%. Moreover, this adsorbent can maintain a removal rate of ≥90% under a wide pH range (6-11) and high ion concentration conditions (1M). The activation and regeneration conditions of the adsorbent of this invention are mild; dye molecules can be removed by elution in a low-concentration alkaline solution only a few times. The adsorbent can be continuously recycled at least 10 times without significant attenuation of adsorption capacity.

The invention discloses a preparation method of a zinc-based metal organic framework material and application of the zinc-based metal organic framework material in adsorption and degradation of methylene blue dye. Belongs to the field of new materials and sensors. According to the zinc-based metal organic framework material, azobenzene tetracarboxylic acid and 2, 6-diaminopurine are taken as ligands, zinc ions are taken as a coordination center, nitric acid is taken as a pH regulator, and the novel regular porous octahedral metal organic framework material is synthesized in one step by utilizing a solvothermal method. The zinc-based metal organic framework material shows relatively strong adsorption and degradation capability on methylene blue, and the adsorption and degradation rate on the methylene blue is as high as 97%; the preparation process of the zinc-based metal organic framework material is simple and safe, the raw materials are cheap, and the zinc-based metal organic framework material has a regular porous octahedral structure, has relatively strong adsorption and degradation capability on methylene blue, and has excellent application and popularization prospects.

The application discloses an electrochemical sensor for detecting IBD protein markers and a preparation method thereof. The electrochemical sensor is composed of a CNT-embedded semi-cured PDMS flexible substrate, deposited gold nanoparticles and a nucleic acid sensing layer. The nucleic acid sensing layer comprises a tumor necrosis factor-alpha sensor, an interleukin 6 sensor, an interleukin 8 sensor, a transforming growth factor beta 1 sensor, a reference electrode and a counter electrode. The tumor necrosis factor-alpha sensor, the interleukin 6 sensor, the interleukin 8 sensor and the transforming growth factor beta 1 sensor all adopt single-stranded aptamers modified by a signal molecule methylene blue, and the nucleotide sequences corresponding to the single-stranded aptamers are SEQ ID NO. 1-4. The application can improve the modulus matching of the sensor and human tissue and excellent biocompatibility, realize accurate detection of low-concentration biomarkers and stable signal output of the sensor under a strain working condition.

According to the method, melamine and urea are used as carbon and nitrogen sources, hollow tubular phosphorus-doped carbon nitride is constructed by adopting a sodium polyphosphate-assisted hydrothermal-thermal polymerization method, molybdenum disulfide is loaded on the surface of P-TCN in a hydrothermal in-situ growth mode, and the hollow tubular phosphorus-doped heterojunction photocatalyst is obtained. The hollow tubular phosphorus-doped heterojunction photocatalyst P-TCN / MoS2 is prepared; the catalyst disclosed by the invention shows excellent degradation performance on ciprofloxacin, levofloxacin, rhodamine B and methylene blue under an illumination condition. The catalyst disclosed by the invention is simple in preparation method, low in cost and easily available in raw materials, and has a wide application prospect in the field of photocatalytic treatment of antibiotic and dye wastewater.