588 results about "Methylene blue" patented technology

The invention relates to the technical field of adsorbents, in particular to modified sodium alginate as well as a preparation method and application thereof. The modified sodium alginate is prepared from the following raw materials: sodium alginate, an activating agent, a sulfhydrylation reagent, a carboxylic acid compound containing unsaturated double bonds and an initiator. Natural polysaccharide sodium alginate is adopted as a raw material, the natural polysaccharide sodium alginate is wide in source, non-toxic and biodegradable, a functional group carboxyl is introduced into sodium alginate through a thiol-ene click reaction, adsorption active sites on the surface of the modified sodium alginate are increased, methylene blue and ciprofloxacin in water can be effectively adsorbed and removed, and the service life of the modified sodium alginate is prolonged. And after multiple times of cyclic adsorption-desorption, relatively good adsorption performance can be maintained.

The invention relates to the technical field of adsorbent preparation, in particular to an ammonia modified porous carbon adsorbent and a preparation method thereof. The preparation method of the ammonia modified porous carbon adsorbent comprises the following steps: carrying out hydrothermal acid leaching on gasified fine residues and an acid solution to obtain acid leaching residues; stirring the acid leaching residues in an alkali solution, washing and drying to obtain a porous carbon material; and dipping the porous carbon material in a tetraethylenepentamine solution, and then drying to obtain the ammonia modified porous carbon adsorbent. The ammonia modified porous carbon adsorbent is successfully constructed by taking a porous carbon material derived from coal gasification fine slag as a substrate and further adopting a surface ammonia functionalization modification strategy. The preparation method is simple, the raw materials are easy to obtain, and the prepared ammonia modified porous carbon adsorbent has a good adsorption effect on methylene blue and hexavalent chromium. According to the method, high-added-value resource utilization of the coal gasification solid waste is achieved, and a solution is provided for treatment of the dye wastewater and the heavy metal wastewater.

The invention relates to the field of scavengers, in particular to a copper-based urea crystal scavenger for a vehicle and a preparation method of the copper-based urea crystal scavenger. The automotive copper-based urea crystal scavenger provided by the invention is prepared from the following raw materials: a copper-based catalyst, a surfactant, a foaming agent, a dispersing agent, a reducing pigment and deionized water, wherein the copper-based catalyst comprises copper acetate, the surfactant comprises AEO-10 and MOA-3PK, and the reducing pigment comprises methylene blue; the prepared automotive copper-based urea crystal scavenger is convenient to use, excellent in urea crystal melting effect and short in effective mileage, the environmental protection property of automobile running can be obviously improved after the automotive copper-based urea crystal scavenger is used, and meanwhile the effect of reducing the use cost of an automobile is remarkable.

The invention discloses a preparation method of a sandwich structure immunosensor based on surface enhanced Raman scattering and application of the sandwich structure immunosensor to detection of an anti-phospholipase A2 receptor antibody, and is characterized by comprising the following steps: sputtering silver ions on a cotton swab / ZIF-67 prepared by an in-situ growth method by adopting an ion sputtering method to obtain a cotton swab / ZIF-67 / Ag NPs composite material; the preparation method comprises the following steps: preparing an antigen solution, then adding the antigen solution into the antigen solution to obtain an immune substrate, adding a methylene blue aqueous solution into a silver nanoparticle solution for reaction, then connecting an anti-human PLA2R IgG antibody to obtain an immune probe, and finally dropwise adding a phosphate buffer solution containing an antibody to be detected onto the immune substrate, and dropwise adding the immune probe solution to the immune substrate adsorbed with the antibody to be detected to obtain the sandwich structure immunosensor based on surface enhanced Raman scattering. The method has the advantages of high sensitivity, good selectivity and strong stability.

The invention relates to a universal method for co-adsorbing perrhenate or pertechnetate by using cationic dye modified activated carbon, and belongs to the technical field of water treatment. In the system provided by the invention, the cationic dye modified activated carbon is used as an adsorbent of perrhenate or pertechnetate, and the cationic dye / activated carbon composite material realizes efficient removal of pollutants by co-adsorbing the perrhenate or pertechnetate in sewage. According to the invention, 12 activated carbon is used as a porous carrier, and after five different cationic dyes are respectively adsorbed, perrhenate radicals can be effectively adsorbed. Particularly, after methylene blue dye is adsorbed, perrhenic acid radicals can be quickly adsorbed within an extremely short time, and the adsorbent has the advantages of large adsorption capacity, good pH stability, high selectivity, good radiation resistance, low cost and the like, and can be applied to actual environmental sewage.

The invention relates to three-dimensional modified polyvinyl alcohol hydrogel as well as a preparation method and application thereof, and relates to the technical field of evaporation materials, few layers of FLG and alpha type Bi2O3 are doped in polyvinyl alcohol hydrogel, and Ppy vapor deposition is performed on the surface of the hydrogel to obtain the three-dimensional modified polyvinyl alcohol hydrogel 3D-PVA coated Bi2O3-FLG / Ppy PEMs. After the material prepared by the method is finally optimized, the evaporation rate reaches 4.03 kg / (m < 2 >. H), meanwhile, the stable evaporation rate of 3.06 kg / (m < 2 >. H) is shown in an evaporation experiment for simulating northern sea water polluted by methylene blue dye, and the degradation effect on pollutants in evaporated water reaches 81% in an illumination experiment. The material prepared by the method not only has high evaporation rate in seawater desalination application, but also shows great potential and application prospect in treatment of seawater polluted by organic dye.

The multi-channel electrochemical micro-fluidic chip detection platform provided by the invention realizes rapid, high-throughput, low-cost, high-sensitivity and specific detection on genital tract pathogens. Specifically, when a target exists, a target recognition probe Cas12a-crRNA compound specifically recognizes a target and forms a Cas12a-crRNA-Target triad, the trans-cleavage activity is activated, an activated Cas12a system cuts a G1 / G2 single-chain region in the signal amplification probe, then encapsulated methylene blue is released, the methylene blue further promotes a methyl bromide redox reaction, signal transduction is promoted, and the target recognition probe Cas12a-crRNA compound can be used for recognizing the target. Therefore, high-sensitivity and high-specificity quantitative detection of pathogens is realized. On the basis, through a multi-channel micro-fluidic chip, high-sensitivity detection of multiple targets can be realized at the same time by placing different crRNA sequences in reaction tanks respectively. In the embodiment of the invention, the multi-target ultra-sensitive detection of CT, CA, TV and NG is successfully realized, and the detection limit is as low as 0.8 fM.

The invention belongs to the field of graphene textile wastewater treatment, and particularly relates to a graphene oxide-based zwitterionic composite filtering film as well as preparation and application thereof. The SBMA is modified to the GO surface through graft polymerization to form a physical intercalation composite structure, so that the hydrophilicity, interlayer spacing and antifouling property of the membrane are remarkably improved. The retention rate of the composite membrane on methylene blue exceeds 99%, the salt separation factor reaches 180.75, and the flux recovery rate (FRR) is kept above 80% after circulation. The membrane preparation process is green and environment-friendly, is suitable for high-efficiency separation of dye and salt in textile wastewater, and has a wide application prospect.

The invention discloses a telomerase detection composition, kit and system based on DNA tetrahedron strand displacement reaction, the composition comprises four single-chain sequence probes T1, T2, T3 and T4 used for assembling into a three-dimensional DNA tetrahedron, and the top of the three-dimensional DNA tetrahedron is provided with a suspension sequence P0; a probe P1 and a probe P2 each comprising a partial complementary sequence of P0; the 5'end of the probe P3 is marked with methylene blue; and a strand displacement reaction primer. The method has high sensitivity and good selectivity, and various cells can be distinguished through telomerase activity; meanwhile, the method also has the capability of screening a telomerase inhibitor; compared with a conventional detection scheme, the method does not need any other enzyme, only needs isothermal one-pot reaction, and can avoid complex operation and expensive reagents, so that the method has huge potential practical value for early diagnosis of telomerase-related diseases and drug research and development.

The invention relates to the technical field of adsorbent preparation and methylene blue adsorption, in particular to application of an ammonia modified porous carbon adsorbent in methylene blue adsorption. An ammonia modified porous carbon adsorbent is added into a water sample containing methylene blue for adsorption, so that the methylene blue in the water sample is removed; the preparation method of the ammonia modified porous carbon adsorbent comprises the following steps: carrying out hydrothermal acid leaching on gasified fine residues and an acid solution to obtain acid leaching residues; stirring the acid leaching residues in an alkali solution, washing and drying to obtain a porous carbon material; and dipping the porous carbon material in a tetraethylenepentamine solution, and then drying to obtain the ammonia modified porous carbon adsorbent. The ammonia modified porous carbon adsorbent is successfully constructed by taking a porous carbon material derived from coal gasification fine slag as a substrate and further adopting a surface ammonia functionalization modification strategy. The ammonia modified porous carbon adsorbent has a good adsorption effect on methylene blue.

The invention discloses a high-entropy spinel ferrite catalyst for efficiently degrading dyes and antibiotics as well as a preparation method and application of the high-entropy spinel ferrite catalyst. The Cu1 / 2Zn1 / 2Fe2 / 3Co2 / 3Ni2 / 3O4 (HEO-FNCCZ) high-entropy spinel ferrite catalyst is prepared by adopting a sol combustion method, the photocatalytic degradation performance of common dyes and antibiotics is evaluated by simulating visible light photocatalysis, and the reaction mechanism of photocatalytic degradation of methylene blue with the best effect is researched. The photocatalytic reaction is effectively promoted by utilizing the catalytic effect of the spinel ferrite, so that environment-friendly pollutant degradation is realized. According to the method, the production process is simple, various organic toxic and harmful substances are not introduced, the method is nontoxic and harmless in the use process, the recovery efficiency of the catalyst exceeds 95%, and the method has the characteristics of remarkable degradation effect and no secondary pollution; the preparation method only relates to the processes of dissolving, calcining, crushing and the like, and is extremely suitable for industrial batch production.

The invention belongs to the technical field of medical instruments, and discloses a digestive endoscopy diseased region accurate positioning and marking device which comprises a controller, an endoscope hose is fixedly connected to the bottom of the controller, and an instrument insertion opening is formed in the right side of the controller. Through the arrangement of the sleeve, the dye tube, the pressurization air bag and the baffle, after an operator observes a diseased region through the endoscope hose, the operator only needs to align the endoscope hose to the diseased region, and by pressing the pressurization air bag, air in the pressurization air bag enters the dye tube through the inner hose; the baffle can be pushed to rotate outwards under the action of air pressure, meanwhile, methylene blue in the dye tube is sprayed out under the action of air flow, a diseased region is marked, the methylene blue can be absorbed by normal mucous membrane cells, and diseased tissue shows dyeing difference due to abnormal cell function and poor absorption capacity, so that the diseased region is marked. Therefore, the effect of accurate positioning is achieved.

The invention discloses a preparation method of a halloysite nanotube adsorption material with sulfonic groups polymerized on the surface, and belongs to the technical field of wastewater treatment materials, the preparation method comprises the following steps: S1, adding 5.0 g of halloysite nanotubes and 5 mL of gamma-methacryloxypropyltrimethoxysilane into 100 mL of toluene; s2, in a magnetic stirrer, condensing and refluxing at 120 DEG C for 10 hours, cooling, filtering, washing with toluene and ethanol for multiple times to remove unreacted KF570, and drying a product in a vacuum drying oven at 60 DEG C for 8 hours to obtain a KH570 modified halloysite nanotube; s3, weighing 1.0 g of HNTs-KH570 as a raw material, dispersing the HNTs-KH570 in 80 mL of distilled water, adding 5.0 g of 2-acrylamide-2-methylpropanesulfonic acid, performing uniform ultrasonic dispersion, and continuously stirring at the temperature of 70 DEG C; and S4, adding 0.15 g of ammonium persulfate into 20 mL of distilled water, and fully stirring to completely dissolve the ammonium persulfate. The adsorption performance of the halloysite nanotube to methylene blue is remarkably improved through sulfonic acid group modification, and the adsorption capacity is improved by 270%.

The invention discloses an electrochemical urine biopsy system for cathepsin B analysis and a detection method of the electrochemical urine biopsy system. The electrochemical urine biopsy system comprises gold nanoparticles AuNPs, a peptide-peptide nucleic acid PNA series probe and an electrochemical sensor. The peptide-peptide nucleic acid tandem probe is combined with gold nanoparticles through a mercaptan modified sequence to form a spherical nanostructure with the diameter of 15 nm, a polypeptide substrate sequence serves as a specific reaction substrate of cathepsin B, and PNA provides stability and a signal shielding function. The electrochemical sensor is based on a cutting enzyme-assisted DNAwalker mechanism, signal amplification is started through methylene blue MB release, and high-sensitivity detection of the released PNA probe is achieved. The system provided by the invention has the characteristics of high specificity, high sensitivity and rapid response, can realize quantitative analysis of cathepsin B through noninvasive urine biopsy, and provides a new technical means for early diagnosis and treatment monitoring of tumors.

The invention belongs to the technical field of piezoelectric catalytic materials, and particularly relates to a piezoelectric catalytic material based on a wood porous structure and a preparation method of the piezoelectric catalytic material. The invention provides a piezoelectric catalytic material based on a wood porous structure. The piezoelectric catalytic material comprises wood with a porous structure, molybdenum disulfide and reduced graphene oxide, wherein the molybdenum disulfide and the reduced graphene oxide are loaded on the porous structure. Molybdenum disulfide, reduced graphene oxide, rGO and MoS2 are loaded on the porous wood to form a nano heterojunction, so that the porous wood has the characteristics of good conductivity and high carrier separation efficiency, carriers (electrons and holes) generated by MoS2 in the piezoelectric process can be effectively captured and transmitted, the piezoelectric catalytic performance of the porous wood is improved, and the preparation method is simple and easy to implement. Under normal-temperature and ultrasonic conditions, the composite material has relatively high degradation efficiency on organic dyes (methylene blue and rhodamine B), and has relatively high cyclic utilization rate.

The invention belongs to the technical field of printing and dyeing wastewater treatment, and particularly relates to a g-C3N4-based adsorbent and a synthesis method thereof.The method comprises the steps that urea is put into a drying oven to be dried, dried urea is obtained, lauryl sodium sulfate is added into the dried urea, mixing and grinding are conducted, a mixture is obtained, then the mixture is transferred into a crucible to be calcined, and a calcined product is obtained; and cooling the calcined product to room temperature, washing and drying to obtain the g-C3N4-based adsorbent. According to the preparation method, the lauryl sodium sulfate is added firstly and then calcined, so that the electronegativity of the product is effectively improved, the electrostatic adsorption capacity to cationic methylene blue is remarkably improved, and the adsorption rate is finally improved.

Methylthioninium salt-containing compositions can include methylthioninium chloride (Methylene Blue) in combination with additional compounds that work cooperatively and / or synergistically to treat various conditions. In one example, the methylthioninium salt-containing composition can include methylthioninium chloride, caffeine, and L-theanine. The methylthioninium chloride and the caffeine can be present at a weight ratio from about 1:75 to about 1:4. the methylthioninium chloride and the L-theanine can be present at a weight ratio from about 1 :200 to about 1:10, and the caffeine and the L- theanine can be present at a weight ratio from about 1 :5 to about 1:1.

The invention relates to a method for preparing a magnetic Fe3O4 / Cu / nitrogen-doped carbon composite material by a one-step method and an application of the magnetic Fe3O4 / Cu / nitrogen-doped carbon composite material, and aims to solve the problems of poor catalytic activity, complex separation and recovery and complex synthesis method of the existing PMS activated catalytic material. According to the method, melamine is selected as a carbon source and a nitrogen source, ferric nitrate and copper sulfate are selected as an iron source and a copper source respectively, and the magnetic Fe3O4 / Cu / nitrogen-doped carbon composite material is synthesized in one step by adopting a high-temperature pyrolysis method. The adsorbent not only has excellent adsorption performance on dye-methylene blue, but also can realize high-efficiency oxidative degradation. The magnetic Fe3O4 / Cu / nitrogen-doped carbon composite material prepared by the invention not only has excellent catalytic performance, but also is easy to magnetically separate and recycle, and is suitable for efficient treatment of dye wastewater. The method is applied to the field of catalytic degradation of wastewater.

The invention discloses a preparation method of a modified adsorption material, and belongs to the field of water treatment. The method comprises the following steps: selecting analytically pure hydrochloric acid with the mass fraction of 37%, preparing a 10% hydrochloric acid solution, adding activated carbon into the hydrochloric acid solution according to the mass-volume ratio of 1g: 10mL, and shaking until the hydrochloric acid is fully contacted and reacted with the activated carbon; after the reaction is finished, washing with a large amount of deionized water until the pH value is close to 7, and then putting the washed activated carbon into a drying oven to obtain activated carbon pretreated by hydrochloric acid; through hydrochloric acid pretreatment, etching and chambering, a pore structure is optimized, functional groups such as nitryl and the like are directionally introduced through potassium nitrate high-temperature activation, a chambering-functionalization synergistic effect is formed, the specific surface area of the activated carbon is increased, the mesopore proportion is increased, the nitrogen element content reaches 2.78%, the adsorption capacity of pollutants such as methylene blue is increased, meanwhile, the raw material cost is low, and the environment friendliness is good. The problems that existing activated carbon is insufficient in pore structure regulation and control, lack of functional groups, low in adsorption efficiency, degraded in regeneration performance and the like are effectively solved.

The invention discloses an IEF-11 / WO3-xS type heterojunction material with full spectrum response as well as a preparation method and application of the IEF-11 / WO3-xS type heterojunction material. The heterojunction is prepared by taking squaric acid, tetrabutyl titanate and WO3-x particles as raw materials, wherein the value range of x in WO3-x is 0 < = xlt; 1. The heterojunction material creatively realizes full-spectrum response from ultraviolet to near infrared through S-type energy band structural design, the separation efficiency of photo-induced electron-hole pairs reaches 2.47 times (compared with WO3-x) and 1.57 times (compared with IEF-11) due to a unique carrier transport mechanism, and strong redox sites are synchronously reserved, so that the material can be applied to the field of photoelectron-hole separation. And compared with a single-component material, the photocatalytic performance is improved by 3.74 times (compared with WO3-x) and 1.41 times (compared with IEF-11). Experiments show that under full-spectrum irradiation, the inactivation rate of the material to escherichia coli can reach 99%, the 120-minute degradation rate of the material to tetracycline (TC) reaches 86%, the material shows broad-spectrum degradation capacity to typical organic pollutants such as chlortetracycline (CTC), rhodamine B (RhB) and methylene blue (MB) in a visible region, and the 120-minute degradation efficiency reaches 77%, 53% and 95% respectively. The heterojunction material is prepared through a simple hydrothermal method, and has important application value in the fields of environmental governance, photocatalytic disinfection and the like.

The present invention belongs to the technical field of rapid detection of drug sensitivity for bacteria. Disclosed are an enrichment culture medium, and a preparation method therefor and the use thereof. The enrichment culture medium comprises the following raw materials in parts by mass: 15.0-20.0 parts of tryptone, 3.5-5.5 parts of peptone, 1.5-3.5 parts of glucose, 5.0-6.5 parts of yeast extract, 2.0-2.5 parts of dipotassium phosphate, 2.0-3.5 parts of disodium hydrogen phosphate, 1.0-1.5 parts of monopotassium phosphate, 2.5-7.5 parts of sodium chloride, 12.0-18.0 parts of proteose peptone, 15.0-22.0 parts of beef heart infusion powder, 8.0-12.0 parts of soya peptone, 1.0-5.0 parts of 0.1% nicotinamide adenine dinucleotide (NAD), 30-100 parts of newborn calf serum inactivated at 56°C for 30 min, 2.0-4.0 parts of mannitol, 3.0-5.0 parts of sodium pyruvate, 0.23-0.45 parts of cycloheximide, 0.8-2.0 parts of 0.05% bromothymol blue, 2.5-4.5 parts of 0.1% methylene blue, and 1000 parts of distilled water, with the pH value of 6.8-7.5. The enrichment culture medium is used to screen effective therapeutic drugs for bacterial diseases in poultry, and is convenient to use and has a single enrichment effect superior to that of respective selective enrichment broths of target bacteria. Therefore, the culture medium can realize rapid proliferation of common pathogenic bacteria in poultry.

The invention discloses a polyacid-based metal viologen framework crystalline material as well as a preparation method and application thereof, and belongs to the technical field of heterogeneous catalytic materials. The crystalline material is synthesized in one step through a hydrothermal method, the molecular formula is Cu2 (H2O) 2 (L) 2 [PW12O40] [OH]. 2H2O, the crystalline material belongs to a monoclinic system, the space group is P21, the cell parameters are alpha = 90 degrees, beta = 114.208 (2) degrees and gamma = 90 degrees, and the structure of the crystalline material is determined through X-ray diffraction. According to the material, accurate compounding of polyacid clusters and metal viologen on the molecular level is achieved, an effective electron transmission channel is constructed, separation of photon-generated carriers is remarkably promoted, and excellent photocatalytic activity is shown. At the ambient temperature, the crystalline material can be used as a catalyst to efficiently degrade dye pollutants (such as methylene blue and rhodamine B) in a water body, the degradation rate within 60 minutes exceeds 90%, and the crystalline material has good cycle stability and structural durability.

The invention discloses a preparation method of methylene blue, which comprises the following steps: by taking phenothiazine as a raw material, carrying out bromination reaction to obtain 3, 7-dibromo-10H-phenothiazine, then carrying out acetylation reaction to obtain 3, 7-dibromo-10-acetyl phenothiazine, then carrying out ammonification reaction to obtain 3, 7-bis (dimethylamino)-10-acetyl phenothiazine, and finally, carrying out amination reaction to obtain 3, 7-bis (dimethylamino)-10-acetyl phenothiazine. And finally hydrolyzing and oxidizing to obtain methylene blue. The method is used for solving the technical problem of low purity of methylene blue.

The invention discloses an indium-based metal-organic framework material based on a pyridine hexacarboxylic acid ligand as well as a preparation method and application of the indium-based metal-organic framework material, the chemical formula is {[NH2Me2] 2 [In4 (mu2-O) (H6L) 2 (H2O) 2]. 3DMF. 10H2O} n, and H6L is an organic ligand 4, 4 ', 4' '-(pyridine-2, 4, 6-triyl) triisophthalic acid. The material has excellent thermal stability and chemical stability, and due to the frame charge characteristic and the excellent pore structure of the material, the metal organic frame material shows efficient dye selective adsorption and separation performance, can selectively adsorb cationic dye methylene blue MB and repel anionic dye methyl orange MO at the same time. In conclusion, a new design thought is developed for the novel indium-based metal organic framework material based on the pyridine hexacarboxylic acid ligand, and meanwhile, a new candidate is provided for developing a high-efficiency dye wastewater purification material.

The invention provides a dual-mode portable biosensor for detecting pseudomonas aeruginosa and application of the dual-mode portable biosensor, and belongs to the technical field of biosensors. The dual-mode portable biosensor comprises a closed bipolar electrode, magnetic beads for detecting pseudomonas aeruginosa, a methylene blue solution, a TCEP solution, a Ru (bpy) 3 < 2 + > solution, boron nitride quantum dots and a TPrA solution. The portable biosensor can rapidly and accurately detect the content of pseudomonas aeruginosa in a sample under low driving voltage, is high in sensitivity, good in specificity, high in anti-interference performance, good in repeatability and convenient to carry, and can be used for field detection. Pseudomonas aeruginosa adsorbed on the detected electrode is effectively killed by adopting copper ions, so that collaborative development of dual functions of detection and sterilization is realized, and environmental pollution is effectively avoided.

The invention discloses a MnFe2O4 / SWAC composite nano-microsphere wave-absorbing material and a preparation method thereof. The preparation method comprises the following steps: firstly, preparing SWAC by taking methylene blue saturated wooden activated carbon as a raw material; then dispersing the porous carbon material SWAC in ethylene glycol, and performing ultrasonic treatment to obtain a mixed solution; the preparation method comprises the following steps: dispersing MnCl2 and FeCl3. 6H2O in a mixed solution, adding PEG 4000 and sodium acetate, stirring, transferring the mixture into a high-pressure kettle, heating for a period of time, filtering, washing the obtained precipitate with ionized water and absolute ethyl alcohol, and drying to obtain the MnFe2O4 / SWAC composite nano-microsphere wave-absorbing material. According to the method, the raw materials are easy to obtain, the cost is low, and the prepared composite nano-microsphere wave-absorbing material has the advantages of being wide in bandwidth and thin in thickness.

The invention discloses a coated carbon material as well as a preparation method and application thereof, and belongs to the technical field of recycling of coated carbon materials. The preparation method comprises the following steps: adding lithium iron phosphate powder into an acid solution, heating to 30-80 DEG C, adding an oxidant or introducing oxygen, reacting at the same temperature for 3-24 hours, carrying out suction filtration, and drying an obtained filter cake to obtain the coated carbon material. The preparation method disclosed by the invention is simple and convenient in process and low in energy consumption, and the prepared coated carbon material can be used for preparing an organic matter adsorbent and an organic matter degradation catalyst and shows excellent performance in adsorption of methylene blue in wastewater and catalytic degradation of sulfonamide antibiotics; the carbon material in the lithium iron phosphate battery is recycled, waste is treated by waste, and the current social environment problem can be effectively solved.

The invention discloses a kit for detecting an A beta oligomer. The kit comprises a nucleic acid aptamer-activator modified streptavidin magnetic bead, a methylene blue nucleic acid signal probe modified streptavidin magnetic bead, a Cas12a-crRNA compound and a synthetic gold nanocluster probe modified glassy carbon electrode. According to the invention, the beta-CD-AuNCs probe with high quantum yield is used as an electrochemiluminescence material, methylene blue is used as an electrochemiluminescence quencher, and through combination with CRISPR / Cas technology, signal change of an ECL reaction system is effectively regulated and controlled, and AbetaO detection is realized. The method has the advantages of strong detection specificity, good selectivity, high sensitivity and strong anti-interference capability on AbetaO, and has good market value.

This invention discloses an amplification-free electrochemical biosensor and method based on CRISPR-Cas13a. The sensor includes an electrochemical biosensor substrate, a dual-channel RNA reporter molecule, a CRISPR-Cas13a reaction system, and a dual-channel differential signal processing module. The working electrode surface is immobilized with a stem-loop structured first reporter molecule and a linear second reporter molecule loaded with gold nanoparticles, labeled with methylene blue and ferrocene signal molecules respectively, and immobilized with a ferrocene-specific capture probe. When target RNA is present, Cas13a is activated and non-specifically cleaves the reporter molecule, reducing the methylene blue signal and enriching and enhancing the ferrocene signal. Background self-calibration is achieved through dual-channel differential ratio calculation. This invention integrates signal attenuation and signal enhancement modes, offering advantages such as nucleic acid amplification-free operation, fast detection speed, high sensitivity, and strong anti-interference capability.

The invention relates to the technical field of dye separation, and discloses a loose nanofiltration membrane and a preparation method and application thereof, the loose nanofiltration membrane comprises a porous support membrane, and a polyesteramide separation layer formed on the porous support membrane through one-step interfacial polymerization of nucleoside monomers and multi-acyl chloride monomers. The nucleoside monomer is one or more than two of adenosine, guanosine and cytidine nucleoside. The nucleoside monomer with a long-chain structure, a branched-chain structure, a rigid twisted structure and a plurality of hydrophilic reaction sites is selected as an interfacial polymerization water-phase monomer, so that the loose nanofiltration membrane is endowed with excellent permeation and separation performance. The membrane has a high rejection rate on dye molecules such as Congo red and methyl blue, also has high water permeability and extremely high dye / inorganic salt separation selectivity, and shows good application potential in the aspects of dye production and purification and dye wastewater recovery.