Method for obtaining number and area of cells based on multicolor fluorescence picture

A technology of cell number and cell area, applied in image data processing, calculation, image enhancement, etc., can solve problems such as the inability to analyze a large number of fluorescent image data efficiently and accurately in batches, and the inability to directly calculate the absolute size of cells by the number and area of ​​cells

Active Publication Date: 2021-05-18
ZHONGSHAN HOSPITAL FUDAN UNIV
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Problems solved by technology

[0003] The technical problem to be solved by the present invention is: the existing methods for counting the number and area of ​​cells in multi-color fluoresc

Method used

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Embodiment Construction

[0019] Below in conjunction with specific embodiment, further illustrate the present invention. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention. In addition, it should be understood that after reading the teachings of the present invention, those skilled in the art can make various changes or modifications to the present invention, and these equivalent forms also fall within the scope defined by the appended claims of the present application.

[0020] The present invention provides a method for counting the number and area of ​​cells based on multicolor fluorescent images, comprising the following steps:

[0021] Step 1. Obtain a multicolor fluorescent picture, and separate the multicolor fluorescent picture into three different pictures according to the three primary colors (blue, green, red);

[0022] Step 2. Process the three images obtained by separation to obtai...

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Abstract

The invention provides a method for obtaining the number and area of cells based on a multicolor fluorescent picture, which is characterized by comprising the following steps of: obtaining the multicolor fluorescent picture; separately processing the three pictures obtained through separation, and obtaining the cell number and the cell area of a single picture; and obtaining the cell number and the cell area of the three pictures obtained by separating the current multicolor fluorescent picture, and carrying out partition counting on all the cell areas according to the size, so that the cell number and the cell area of the current multicolor fluorescent picture are obtained after the cell number and the cell area of the three pictures are combined. The method for obtaining the number of fluorescent cells provided by the invention can be used for accurately analyzing the number and size of cells in a fluorescent picture in batches, so that the problems of low counting and area statistical efficiency and accuracy, complicated operation and the like of the existing multi-color fluorescent staining cell counting methods such as immunofluorescence, TUNEL, EdU and the like are solved.

Description

technical field [0001] The invention relates to a method for counting the number and area of ​​cells in a multicolor fluorescent picture. Background technique [0002] In order to count the number and area of ​​cells in multicolor fluorescent images, the existing method is to use image processing software such as ImageJ to import the three primary color images of multicolor fluorescent images into the software one by one, and then count the cells in the multicolor fluorescent images by setting color thresholds number and area. Existing methods cannot directly calculate the absolute size of cells, and cannot efficiently analyze a large amount of fluorescent image data in batches. Contents of the invention [0003] The technical problem to be solved by the present invention is that the existing methods for counting the number and area of ​​cells in multi-color fluorescent images cannot directly calculate the absolute size of cells, and cannot efficiently and accurately anal...

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Application Information

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IPC IPC(8): G06T7/00G06T7/13G06T7/62
CPCG06T7/0012G06T7/13G06T7/62G06T2207/10064G06T2207/10056G06T2207/30004G06T2207/30242
Inventor 葛均波张志伟杨向东丁素玲朱小伟
Owner ZHONGSHAN HOSPITAL FUDAN UNIV
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