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NO<3><-> ion detection reagent and application thereof

An ion detection and detection reagent technology, applied in the field of NO3- ion detection reagents, can solve the problems of large ionic radius and few fluorescent probes, and achieve good membrane penetration, high sensitivity fluorescence enhancement, and high luminescence yield. rate effect

Active Publication Date: 2015-10-07
北京传奇优声文化传媒有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the high solvation free energy and large ionic radius of anions, so far, there are not many fluorescent probes that can be applied to the environment or in living cells for anion fluorescence imaging.

Method used

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  • NO&lt;3&gt;&lt;-&gt; ion detection reagent and application thereof
  • NO&lt;3&gt;&lt;-&gt; ion detection reagent and application thereof
  • NO&lt;3&gt;&lt;-&gt; ion detection reagent and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Weigh 390 mg of 9,10-bispyridylvinyl anthracene, put it into a 25 mL round bottom flask, add 8 mL of anhydrous N,N-dimethylformamide, add 400 mg of bromooctane, and heat to reflux for 24h . After cooling, filter, and rinse the solid with dichloromethane and petroleum ether, then dissolve the recrystallized red solid powder with acetonitrile solution a (230 mg, 30% yield).

Embodiment 2

[0035] (1) Probe a Solution preparation: weigh 7.7 mg of a , dissolved in double distilled water, and prepared into 10.0 mL solution with a concentration of 1.00×10 -3 mol L -1 ; Dilute stepwise with distilled water to 1.00×10 as needed -5 mol · L -1 concentration; (2) NO 3 - Standard solution: Weigh 30.4 mg of tetrabutylammonium nitrate, dissolve it in twice distilled water, and prepare a 100mL solution, NO 3 - The concentration is 2.00×10 -3 mol L -1 ; Dilute step by step with twice-distilled water to an appropriate concentration as required; the preparation of other coexisting metal ion solutions is the same.

Embodiment 3

[0037]Add probe to 10.0 mL volumetric flask a water stock solution (1.00 x 10 -4 mol L -1 , 1mL), anion NO 3 - (1.00×10 -3 mol L -1 , 1 mL). Dilute to the mark with double-distilled aqueous solution, shake well, and transfer to a 3cm quartz cuvette for fluorescence spectrum measurement. probe a The excitation wavelength of solution fluorescence spectrometry is 470 nm. In aqueous solution, the probe a (1.00×10 -5 mol L -1 ) itself has very weak fluorescence emission, and basically no fluorescence can be observed under ultraviolet light. Join NO 3 - (1.00×10 -4 mol L -1 ) after making the probe a The fluorescence of the solution at 575 nm was significantly enhanced, and other experimental anions F - , Cl - , Br - , HSO 4 - , SO 4 2- , AcO - , BF 4 - , H 2 PO 4 - pair of probes a None of the solutions showed a significant signal response, indicating that the probe a only for NO 3 - Selective fluorescence enhanced detection response performanc...

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Abstract

The invention relates to an NO<3><-> ion detection reagent and an application thereof, and belongs to the technical field of analysis chemistry. A tiny amount of NO<3><-> in a neutral aqueous solution is detected through a fluorescence method by using a 9,10-dipyridylvinyl anthracene cation derivative as a fluorescence probe a. When NO<3><-> in the aqueous solution is determined, the fluorescence intensity at 575nm is determined by using 470nm as fluorescence excitation wavelength, the detection concentration linear range is 1.5*10<-7>-2.1*10<-5>mol.L<-1>, and the lowest detection limit is 10<-8>mol.L<-1>. After the probe a and NO<3><-> are intracellularly stained in a living cell imaging, a fluorescence inverted microscope detection result shows that clear yellow fluorescence cell distribution exists, a cell image is shot to obtain a clear cell contour image, and staining has specific area selective aggregation capability.

Description

technical field [0001] The invention belongs to the field of organic synthesis and analytical chemistry, specifically a NO 3 - Ion detection reagents and applications. Background technique [0002] Anions play important roles in biological and environmental systems. For example, nitrate ions can oxidize normal hemoglobin in the human body and lose the ability to transport oxygen, and can also react with secondary amine compounds to generate carcinogenic nitrosamine compounds. According to reports, when the nitrate content per liter of water reaches tens of milligrams, it may lead to an increased risk of infants suffering from methemoglobinemia. Therefore, the determination of nitrate ion has important practical significance. At present, the methods for determining nitrate ion include multi-wavelength method, second derivative method, flow injection method, ion chromatography, polarography and so on. Fluorescence spectroscopy has attracted more and more attention due to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/06C07D213/06G01N21/64
Inventor 倪新龙陈仕焰白青鸿
Owner 北京传奇优声文化传媒有限公司
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