The invention belongs to the technical field of
plant genetic engineering, and particularly discloses a
nitrate ion transport channel
gene PbrSLAH3b and application of the
nitrate ion transport channel
gene PbrSLAH3b in promoting growth of
pear pollen tubules. The method comprises the following steps: extracting
total RNA (Ribonucleic Acid) of Dangshan pears from
pollen of Dangshan pears; carrying out reverse transcription on the
RNA to obtain cDNA (
complementary deoxyribonucleic acid); and by taking the cDNA as a template, carrying out amplification to obtain the
pear pollen tube
nitrate transport channel
gene PbrSLAH3b, the PbrSLAH3b gene provided by the invention belongs to a slow anion channel SLAC / SLAH family member, the PbrSLAH3b is remarkably up-regulated in expression in the early growth stage of a
pollen tube, and the PbrSLAH3b is positioned in a
plasma membrane. The invention verifies that PbrSLAH3b can form a functional anion channel with NO3 <-> selectivity under the condition of not depending on
proton gradient or
phosphorylation state, and the PbrSLAH3b has relatively strong selectivity on NO3 <->. Therefore, the PbrSLAH3b gene can mediate the absorption and transport of the
pollen tube to NO3 <-> and maintain the polarity growth
directivity of the
pollen tube, and has important application value for revealing the
ion regulation mechanism of the growth of the
fruit tree pollen tube and improving the reproductive development of the
fruit tree.