The invention relates to the field of traditional Chinese medicinal starters and health foods, in particular to an antioxidant and antineoplastic traditional Chinese medicinal starter and a preparation method thereof. The traditional Chinese medicinal starter is prepared from radix pseudostellariae, semen coicis, agaricus bisporus, wolfberry fruit, fiveleaf gynostemma herb, radix ginseng, radix rubiae, fructus gardeniae, selenium, chromium and zinc through the process steps of aerobic culture, extraction, fermentation, sterilization, purification, concentration and the like, and is preferablyin an oral liquid dosage form. Through addition of the inorganic trace elements of selenium and zinc into the agaricus bisporus, and aseptic culture, inorganic selenium and inorganic zinc are effectively converted into organic selenium and organic zinc, the organic selenium and organic zinc are enriched in agaricus bisporus sporocarp, the agaricus bisporus sporocarp is fermented together with Chinese medicinal herbs, and the fermentation product is extracted to obtain mixed microparticles which organically combine fungal polysaccharides with trace elements. The mixed microparticles have good oxidation resistance and cancer cell growth inhibition function. As proved by tests, compared with existing products, the traditional Chinese medicinal starter is remarkably reduced in toxic and side effects, and is greatly improved in antioxidant and anti-tumor effects.