12 results about "Peroxynitrous acid" patented technology

The invention belongs to the technical field of fluorescent probes, and particularly relates to a preparation method and property research of a dual-response fluorescent probe for detecting polarity and peroxynitrite. The preparation method of the fluorescent probe is simple, the product yield is high, and the fluorescent probe is suitable for large-scale popularization and application. The present invention relates to a method for preparing a probe (Z)-4-(1-cyano-2-(6-(4-(diphenylamino) phenyl)-4-phenyl-4H-dithieno [3, 2-b: 2 ', 3'-d] pyrrol-2-yl) vinyl)-1-(4-(4, 4, 5, 5-tetramethyl-1, 3, 2-dioxaborolan-2-yl) benzyl) pyridine-1-onium bromide (DTBP). When excitation is carried out at 600 nanometers, the fluorescence intensity is gradually enhanced along with the reduction of the polarity of the solution; in a solvent of PBS and acetone in equal proportion, when excitation is carried out at 470 nanometers, the probe reacts with ONOO to generate strong fluorescence. Along with the increase of the ONOO concentration, the fluorescence intensity is gradually enhanced. The probe can specifically detect ONOO, and has a good linear relationship. The probe also has relatively strong fluorescence response to polarity, and the fluorescence emission intensity is gradually increased along with the reduction of the polarity.

The application belongs to the field of fluorescent sensing, and discloses a sulfur rhodamine peroxynitrite probe containing an indole bottom ring, a preparation method and application thereof. The new compound SR-In of the application is not fluorescent due to PET effect, and generates a new fluorescent substance, thioxanthone, after reacting with peroxynitrite. Since the spectral range of sulfur rhodamine and thioxanthone is completely different, the fluorescent signal can be greatly changed before and after detection, and high-sensitivity detection of peroxynitrite is realized. In addition, the indole bottom ring in the structure of SR-In can only be combined with peroxynitrite and oxidized into thioxanthone, so SR-In does not react with other common active oxygen in the organism, and good peroxynitrite selectivity is shown.

The invention relates to a biomedical material, and discloses a preparation method of an MOF (Metal Organic Framework) composite fiber membrane for light-controlled release of NO, which comprises the following steps: 1) ultrasonically dispersing meso-tetra (4-carboxyphenyl) porphin, a regulator and metal salt in an organic solvent, and heating for reaction to obtain porphyrinyl MOF; (2) dispersing the porphyrinyl MOF and an NO donor L-arginine into an ethanol water solution, and reacting to obtain Arg-MOF; 3) ultrasonically dispersing the Arg-MOF in a dimethyl sulfoxide / dichloromethane mixed solvent, adding polylactic acid, and uniformly stirring to obtain a spinning solution; and carrying out electrostatic spinning and drying to obtain the MOF composite fiber membrane capable of releasing NO in a light-operated manner. The composite fiber membrane disclosed by the invention is irradiated by near-infrared light, ROS and NO can be synchronously generated, peroxynitrite with strong oxidizing property is further generated, and efficient synergistic killing of bacteria is realized. In addition, the composite fiber membrane is uniform and stable in structure, particles are good in dispersity and not prone to falling off, and the synergy between the porphyrinyl MOF and the L-arginine is good.

This invention relates to a class of activation-triggered in-situ anchored mitochondrial-targeted fluorescent probes for peroxynitrite anions and their applications. Specifically, the probes of this invention exhibit excellent detection performance for peroxynitrite anions in a pure water system. This is mainly manifested in: achieving precise quantitative detection of ONOOˉ in mitochondria, with significant changes in the fluorescence spectrum generated by the reaction between the probe and ONOOˉ; possessing high selectivity, enabling highly selective detection of ONOOˉ in complex biological environments, with response intensity and speed exceeding those of other analytes; rapid reaction and high sensitivity, with a low detection limit for ONOOˉ; simultaneously, it can respond in a pure water system, making it environmentally friendly; most importantly, this probe can detect peroxynitrite anions in mitochondria and achieve long-term in-situ tracer imaging through covalent binding.

This invention proposes a ratiometric fluorescent probe molecule for detecting peroxynitrite, its preparation method, and its application, aiming to solve a problem in the technical field of fluorescent sensing materials. The ratiometric fluorescent probe molecules of this invention are HPIP-T-BnB and HPIP-K-BnB. The structural formulas of HPIP-T-BnB and HPIP-K-BnB are: [Insert structural formula here], and the structural formulas of HPIP-K-BnB are: [Insert structural formula here]. The preparation method includes the following steps: 4'-(diphenylamino)-4-(imidazo[1,2-a]pyridin-2-yl)-[1,1'-biphenyl]-3-ol or 4'-(9H-carbazole-9-yl)-4-(imidazo[1,2-a]pyridin-2-yl)-[1,1'-biphenyl]-3-ol undergoes a nucleophilic substitution reaction with 2-(4-(bromomethyl)phenyl)-4,4,5,5-tetramethyl-1,3,2-dioxoborhecyclopentane to obtain HPIP-T-BnB and HPIP-K-BnB. The probes prepared in this invention can detect ONOO⁻ with high selectivity, high sensitivity, and quantification, and have been successfully applied to ratiometric fluorescence imaging of exogenous and endogenous ONOO⁻ in live cells. This provides a reliable and crucial tool for mechanism research and drug development in related diseases.

An atomizing method using an atomizer in which peroxynitrous acid is sustainedly generated through plasma activated mist, pH of the plasma activated mist being in a range from 2 to 5, wherein the plasma activated mist has been generated from an aqueous solution containing nitrite, concentration of the nitrite being in a range from 10 micromolar to 10 milli-molar.

The invention discloses a method for treating hexamethylenediamine in nylon 66 wastewater based on a Fenton oxidative coupling biological method, which is characterized in that aldehydes in cyclohexanone process wastewater are utilized to reduce nitrate to generate nitrous acid, and a calcium peroxide, trisodium citrate and ferrite slow-release catalytic system is adopted as a characteristic. The stable supply of an oxidant and the efficient generation of nitrous acid are realized, the low and constant hydrogen peroxide concentration in the solution can be maintained, the hydrogen peroxide and nitrous acid preferentially react to generate peroxynitrous acid with extremely high oxidability, and the substance and hydroxyl free radicals have a synergistic effect, so that the precise and efficient degradation of hexamethylenediamine molecules can be realized, and the degradation efficiency of the hexamethylenediamine is improved. The biotoxicity of hexamethylenediamine is thoroughly broken, the biodegradability of wastewater is remarkably improved, and organic nitrogen is converted into nitrogen which can be easily utilized by microorganisms, so that efficient and stable reaction conditions are created for subsequent anaerobic biological treatment, and the complementary advantages of chemical oxidation and biodegradation are finally realized.

The application discloses a method for treating hexamethylene diamine in nylon 66 wastewater based on Fenton oxidation coupling biological method, and the method is characterized by utilizing aldehyde substances in cyclohexanone process wastewater to reduce nitrate to generate nitrous acid, and adopting a calcium peroxide, trisodium citrate and ferrous salt slow-release catalytic system to realize stable supply of oxidizing agent and efficient generation of nitrous acid, so that a low and constant hydrogen peroxide concentration in the solution can be maintained, the hydrogen peroxide concentration reacts with nitrous acid to generate peroxynitrous acid with extremely strong oxidizing property, the substance cooperates with hydroxyl radicals to realize precise and efficient degradation of hexamethylene diamine molecules, not only the biological toxicity of hexamethylene diamine is completely broken, the wastewater biodegradability is significantly improved, but also organic nitrogen is converted into nitrogen forms which are easy to be utilized by microorganisms, so that efficient and stable reaction conditions are created for subsequent anaerobic biological treatment, and finally the advantages of chemical oxidation and biological degradation are complemented.

The invention discloses a peroxynitrite near-infrared fluorescent probe as well as a preparation method and application thereof, and belongs to the technical field of peroxynitrite near-infrared fluorescent probes. According to the technical scheme, the peroxynitrite near-infrared fluorescent probe is characterized in that the structural formula of the peroxynitrite near-infrared fluorescent probe is shown in the specification. The invention further specifically discloses a preparation method of the peroxynitrite near-infrared fluorescent probe and application of the peroxynitrite near-infrared fluorescent probe to selective detection of peroxynitrite in an aqueous solution, a biological cell system and animal liver tissue. The near-infrared fluorescent probe disclosed by the invention has the advantages of fluorescence emission in a near-infrared region, good selectivity, low detection limit and the like.

The invention discloses a cyanine fluorescent compound as well as a preparation method and application thereof, and belongs to the technical field of fine chemical engineering. The structure of the cyanine fluorescent compound is shown as a formula (I), and the cyanine fluorescent compound can be used for detecting ONOO. The luminescent mechanism of the cyanine fluorescent compound is an intramolecular charge transfer (ICT) mechanism, the emission wavelength is 800nm, the cyanine fluorescent compound is connected with a diphenyl hypophosphite group, in the presence of peroxynitrite ONOO, the diphenyl hypophosphite group leaves through a high-selectivity addition-elimination way, the ICT effect is changed, the emission wavelength is blue-shifted to 633nm, and the cyanine fluorescent compound has the advantages that the fluorescence intensity of the cyanine fluorescent compound is improved; ratio-type fluorescence detection of ONOO is realized, and the detection limit reaches 0.57 mu M. (I)

The invention provides an application of activated water in promotion of crop growth, working gas is introduced into a plasma generator for reaction to obtain plasma gas, the plasma gas is introduced into a water body for treatment to obtain plasma activated water, and then the plasma activated water is subjected to light-shielding standing for 10-50 h to obtain the activated water, or the plasma gas and ozone are mixed for reaction and then are introduced into the water body for treatment, and the activated water is obtained. The activated water can effectively reduce the content of peroxynitrous acid, and can promote the growth of crops.

The invention discloses a fluorescent probe for rapidly and highly sensitively detecting peroxynitrite as well as a synthesis method and application of the fluorescent probe, and belongs to the technical field of chemical analysis and detection. The probe is obtained by condensing a halogen-substituted pyrone large pi system and a diphenyl phosphino compound, and has the following structure: a fluorophore of the probe is a halogen-substituted pyrone large pi system skeleton structure, and a response group to peroxynitrite is a diphenyl phosphino unit. The ultraviolet absorption peak of a single probe in a solution is located at-395nm, no obvious fluorescence is emitted, after the probe reacts with peroxynitrite, the ultraviolet absorption peak of the probe is subjected to red shift to-485nm, and strong fluorescence is emitted at-610nm. The probe molecule has the advantages of high speed, high selectivity, high sensitivity and the like in response to peroxynitrite, the reaction can be completed within 2 minutes, the concentration range of peroxynitrite detected in a solution is 0.03-10 [mu] mol.L <-1 >, and the detection limit is 8 nmol.L <-1 >. In addition, the probe can also be used for detecting peroxynitrite in cells.