25 results about "Peroxynitrous acid" patented technology

The invention belongs to the technical field of fluorescent probes, and particularly relates to a preparation method and property research of a dual-response fluorescent probe for detecting polarity and peroxynitrite. The preparation method of the fluorescent probe is simple, the product yield is high, and the fluorescent probe is suitable for large-scale popularization and application. The present invention relates to a method for preparing a probe (Z)-4-(1-cyano-2-(6-(4-(diphenylamino) phenyl)-4-phenyl-4H-dithieno [3, 2-b: 2 ', 3'-d] pyrrol-2-yl) vinyl)-1-(4-(4, 4, 5, 5-tetramethyl-1, 3, 2-dioxaborolan-2-yl) benzyl) pyridine-1-onium bromide (DTBP). When excitation is carried out at 600 nanometers, the fluorescence intensity is gradually enhanced along with the reduction of the polarity of the solution; in a solvent of PBS and acetone in equal proportion, when excitation is carried out at 470 nanometers, the probe reacts with ONOO to generate strong fluorescence. Along with the increase of the ONOO concentration, the fluorescence intensity is gradually enhanced. The probe can specifically detect ONOO, and has a good linear relationship. The probe also has relatively strong fluorescence response to polarity, and the fluorescence emission intensity is gradually increased along with the reduction of the polarity.

The application belongs to the technical field of fluorescent probe, and particularly relates to a preparation method and property research of a fluorescent probe for simultaneously detecting polarity and peroxynitrite double response. The preparation method of the fluorescent probe is simple, the product yield is high, and the method is suitable for scale popularization and use. The probe (E)-1-(2-carboxyethyl)-4-(2-(5-(4-(diphenylamino)phenyl)thiophene-2-yl)vinyl)quinolin-1-ium iodide (PBQI) is gradually enhanced in fluorescence intensity when being excited at 520 nanometers along with the decrease of solution polarity; when being excited at 400 nanometers in a low-polarity environment, the probe reacts with peroxynitrite to generate strong fluorescence. The fluorescence intensity is gradually enhanced along with the increase of peroxynitrite concentration. The probe can detect peroxynitrite in a low-polarity environment, and has a good linear ratio relationship. The probe also has a strong fluorescence response to polarity, and the fluorescence emission intensity is gradually increased along with the decrease of polarity.

The application belongs to the field of fluorescent sensing, and discloses a sulfur rhodamine peroxynitrite probe containing an indole bottom ring, a preparation method and application thereof. The new compound SR-In of the application is not fluorescent due to PET effect, and generates a new fluorescent substance, thioxanthone, after reacting with peroxynitrite. Since the spectral range of sulfur rhodamine and thioxanthone is completely different, the fluorescent signal can be greatly changed before and after detection, and high-sensitivity detection of peroxynitrite is realized. In addition, the indole bottom ring in the structure of SR-In can only be combined with peroxynitrite and oxidized into thioxanthone, so SR-In does not react with other common active oxygen in the organism, and good peroxynitrite selectivity is shown.

A naphthaleneimide compound, its preparation method, and application. The present invention provides a naphthaleneimide compound, the structure of which is shown in Formula I: R is selected from one of the following: The present invention also provides a method for preparing the naphthaleneimide compound. The compound has the ability to accurately trace mitochondrial autophagy and has the ability to release peroxynitrite (ONOO) ‑ The responsiveness and mitochondrial targeting capabilities of the ELISA can be applied to prepare a kit for tracking oxidative stress-induced mitophagy and / or peroxynitrite activation.

The invention relates to a peroxynitrite anion-targeting fluorescent probe based on activation-triggering in-situ anchored mitochondria and application of the peroxynitrite anion-targeting fluorescent probe. Specifically, the probe disclosed by the invention can show excellent detection performance on peroxynitrite anions in a pure water system. The method is mainly characterized in that accurate and quantitative detection of ONOO <-> in mitochondria is realized, and the fluorescence spectrum generated by the reaction of the probe and ONOO <-> changes remarkably; high selectivity is achieved, high-selectivity detection on ONOO <-> can be achieved in a complex biological environment, and the response strength and speed are both higher than those of other analytes; the reaction is rapid, the sensitivity is high, and the detection limit on ONOO <-> is low; meanwhile, response can be carried out in a pure water system, and the method is environment-friendly; most importantly, the probe can realize detection of peroxynitrite anions in mitochondria, and can realize long-time in-situ tracing imaging through covalent binding.

The present invention provides a compound represented by formula A which can be used as a compound for preventing and treating oxidative damage caused by ischemia-reperfusion and a preparation method thereof. 10 As defined herein. The compounds of the present invention can react with peroxynitrite in the blood and further release carbon monoxide, thereby achieving anti-inflammatory and antioxidant effects. Tests of such compounds at the cellular level and in vivo level in mice show that they have achieved good therapeutic effects on a number of key evaluation indicators including brain near-death area, brain edema, blood-brain barrier, and neurological function scores, and have important potential for drug development.

The invention relates to a biomedical material, and discloses a preparation method of an MOF (Metal Organic Framework) composite fiber membrane for light-controlled release of NO, which comprises the following steps: 1) ultrasonically dispersing meso-tetra (4-carboxyphenyl) porphin, a regulator and metal salt in an organic solvent, and heating for reaction to obtain porphyrinyl MOF; (2) dispersing the porphyrinyl MOF and an NO donor L-arginine into an ethanol water solution, and reacting to obtain Arg-MOF; 3) ultrasonically dispersing the Arg-MOF in a dimethyl sulfoxide / dichloromethane mixed solvent, adding polylactic acid, and uniformly stirring to obtain a spinning solution; and carrying out electrostatic spinning and drying to obtain the MOF composite fiber membrane capable of releasing NO in a light-operated manner. The composite fiber membrane disclosed by the invention is irradiated by near-infrared light, ROS and NO can be synchronously generated, peroxynitrite with strong oxidizing property is further generated, and efficient synergistic killing of bacteria is realized. In addition, the composite fiber membrane is uniform and stable in structure, particles are good in dispersity and not prone to falling off, and the synergy between the porphyrinyl MOF and the L-arginine is good.

The present invention discloses a fluorescence detection method of peroxynitrite ion and its application. A method for detecting peroxynitrite ion by a nanocomposite material comprises the following steps: mixing a peroxynitrite ion standard sample with a carbon quantum dot nanocomposite material solution containing fluorescent carbon quantum dots A and fluorescent carbon quantum dots B, exciting, measuring the displacement value of the emission peak at 495nm, and obtaining a linear equation between the peroxynitrite ion concentration and the wavelength shift value; mixing the sample to be tested with the carbon quantum dot nanocomposite material solution, exciting, measuring the displacement value, and obtaining the peroxynitrite ion concentration by comparing the linear equation; the fluorescent carbon quantum dots A are prepared by crocin T hydrothermal method; the fluorescent carbon quantum dots B are prepared by pyronine Y hydrothermal method. The present invention constructs a wavelength-shift-type sensing system for detecting peroxynitrite ions, which can realize the selective detection of peroxynitrite ions and has broad application prospects.

This invention relates to a class of activation-triggered in-situ anchored mitochondrial-targeted fluorescent probes for peroxynitrite anions and their applications. Specifically, the probes of this invention exhibit excellent detection performance for peroxynitrite anions in a pure water system. This is mainly manifested in: achieving precise quantitative detection of ONOOˉ in mitochondria, with significant changes in the fluorescence spectrum generated by the reaction between the probe and ONOOˉ; possessing high selectivity, enabling highly selective detection of ONOOˉ in complex biological environments, with response intensity and speed exceeding those of other analytes; rapid reaction and high sensitivity, with a low detection limit for ONOOˉ; simultaneously, it can respond in a pure water system, making it environmentally friendly; most importantly, this probe can detect peroxynitrite anions in mitochondria and achieve long-term in-situ tracer imaging through covalent binding.

This invention proposes a ratiometric fluorescent probe molecule for detecting peroxynitrite, its preparation method, and its application, aiming to solve a problem in the technical field of fluorescent sensing materials. The ratiometric fluorescent probe molecules of this invention are HPIP-T-BnB and HPIP-K-BnB. The structural formulas of HPIP-T-BnB and HPIP-K-BnB are: [Insert structural formula here], and the structural formulas of HPIP-K-BnB are: [Insert structural formula here]. The preparation method includes the following steps: 4'-(diphenylamino)-4-(imidazo[1,2-a]pyridin-2-yl)-[1,1'-biphenyl]-3-ol or 4'-(9H-carbazole-9-yl)-4-(imidazo[1,2-a]pyridin-2-yl)-[1,1'-biphenyl]-3-ol undergoes a nucleophilic substitution reaction with 2-(4-(bromomethyl)phenyl)-4,4,5,5-tetramethyl-1,3,2-dioxoborhecyclopentane to obtain HPIP-T-BnB and HPIP-K-BnB. The probes prepared in this invention can detect ONOO⁻ with high selectivity, high sensitivity, and quantification, and have been successfully applied to ratiometric fluorescence imaging of exogenous and endogenous ONOO⁻ in live cells. This provides a reliable and crucial tool for mechanism research and drug development in related diseases.

The invention provides a hydrazino near-infrared fluorescent probe and application of the hydrazino near-infrared fluorescent probe in detection of peroxynitrite, and belongs to the field of biological detection. The emission wavelength of the hydrazino near-infrared fluorescent probe developed by the invention is 730nm, and the hydrazino near-infrared fluorescent probe has high selectivity, high sensitivity (the detection limit is as low as 91nM) and quick response ability to ONOO-, and has low cytotoxicity and excellent in-vivo imaging ability, so that visual observation of ONOO-in a living body CIRI model mouse body is successfully realized; the method has great potential in the aspect of real-time tracking of ONOO-related pathological states.

The invention discloses a mass spectrum imaging probe for detecting peroxynitrite as well as a preparation method and application of the mass spectrum imaging probe. The invention provides a compound with a novel structure, the hemicyanine structure of the compound has a positive electric center, and the compound can be used as a mass spectrum detection label to capture a signal; the tetrazine structure of the compound reacts with peroxynitrite to generate an oxadiazole structure, the oxadiazole structure can also be detected by mass spectrometry, the response abundance of the oxadiazole structure is obviously higher than that of the oxadiazole structure after the oxadiazole structure reacts with other common active oxygen and active nitrogen, and the oxadiazole structure shows excellent peroxynitrite selectivity and can be used as a mass spectrometry probe for detecting the peroxynitrite level in a biological sample. The probe can detect the signal intensity of a corresponding product through a mass spectrum channel to evaluate the generation of peroxynitrite in an in-vivo sample, and the spatial distribution of peroxynitrite in a biological sample (such as ischemia-reperfusion injury liver tissue) is rapidly, simply and accurately analyzed by virtue of a mass spectrum imaging technology. The compound as well as the preparation method and the application thereof are not disclosed in the prior art.

An atomizing method using an atomizer in which peroxynitrous acid is sustainedly generated through plasma activated mist, pH of the plasma activated mist being in a range from 2 to 5, wherein the plasma activated mist has been generated from an aqueous solution containing nitrite, concentration of the nitrite being in a range from 10 micromolar to 10 milli-molar.

The invention discloses a nano-composite as well as a preparation method and application thereof, and relates to the technical field of sterilization, the nano-composite comprises a porphyrin metal organic framework and L-arginine loaded on the porphyrin metal organic framework. Wherein the porphyrin metal organic framework can generate singlet oxygen under the action of visible light so as to play a bactericidal role, and the L-arginine loaded on the porphyrin metal organic framework can be used as a nitric oxide generator so as to play a bactericidal role; in addition, the generated singlet oxygen and nitric oxide are further combined to generate peroxynitrite anion free radicals, so that strong oxidative damage can be caused, and the sterilization effect is further enhanced. Therefore, the nano-composite provided by the invention has a relatively strong bactericidal effect, and meanwhile, no antibiotics are added, so that the induction effect on bacterial drug resistance is reduced.

The invention aims to disclose a preparation method and application of a fluorescent probe for efficiently detecting peroxynitroso. Peroxynitrite groups are one of active reactive oxygen and active nitrogen species, and have a wide range of attention due to high oxidation and nitration capabilities. The peroxynitrite is used as an important physiological activation factor and signal molecule, and can regulate the dynamic redox dynamic balance in a living body. However, when the content of the peroxynitrite is too high, the peroxynitrite can damage protein, nucleic acid and amino acid in cells, so that serious diseases such as neurodegenerative diseases and the like are caused in organisms. Fluorescence sensing and fluorescence imaging can mark and track molecules with wide biological activity, and have good temporal and spatial resolution. Therefore, researchers develop a plurality of small molecular fluorescent probes for detecting and characterizing nitrite peroxide. Coumarin has unique fluorescence, and the fluorescence characteristic of coumarin depends on the structure of coumarin to a great extent, so that the modification of coumarin becomes a basis for constructing an efficient fluorescent probe. Design, synthesis and characterization of a novel coumarin-based fluorescent probe are introduced, and the novel coumarin-based fluorescent probe is used for efficient detection of peroxynitrite.

The invention discloses a method for treating hexamethylenediamine in nylon 66 wastewater based on a Fenton oxidative coupling biological method, which is characterized in that aldehydes in cyclohexanone process wastewater are utilized to reduce nitrate to generate nitrous acid, and a calcium peroxide, trisodium citrate and ferrite slow-release catalytic system is adopted as a characteristic. The stable supply of an oxidant and the efficient generation of nitrous acid are realized, the low and constant hydrogen peroxide concentration in the solution can be maintained, the hydrogen peroxide and nitrous acid preferentially react to generate peroxynitrous acid with extremely high oxidability, and the substance and hydroxyl free radicals have a synergistic effect, so that the precise and efficient degradation of hexamethylenediamine molecules can be realized, and the degradation efficiency of the hexamethylenediamine is improved. The biotoxicity of hexamethylenediamine is thoroughly broken, the biodegradability of wastewater is remarkably improved, and organic nitrogen is converted into nitrogen which can be easily utilized by microorganisms, so that efficient and stable reaction conditions are created for subsequent anaerobic biological treatment, and the complementary advantages of chemical oxidation and biodegradation are finally realized.

An atomizing method comprises providing an atomizer including a mist generator and a plasma producer. The plasma producer includes a tube of dielectric material, an external electrode on an outside surface of the tube of dielectric material, an internal electrode on an inside surface of the tube of dielectric material, and a high-frequency power supply for applying a high-frequency voltage between the external electrode and the internal electrode. Mist from the mist generator is plasma-activated while passing through the tube of dielectric material and emitted from an end of the tube of dielectric material. The external electrode and the internal electrode are located such that location of the external electrode and location of the internal electrode does not overlap or merely partially overlap in the longitudinal direction of the tube of dielectric material. Peroxynitrous acid is continuously generated by generating hydrogen peroxide and nitrous acid using plasma-activated water mist.

The application discloses a method for treating hexamethylene diamine in nylon 66 wastewater based on Fenton oxidation coupling biological method, and the method is characterized by utilizing aldehyde substances in cyclohexanone process wastewater to reduce nitrate to generate nitrous acid, and adopting a calcium peroxide, trisodium citrate and ferrous salt slow-release catalytic system to realize stable supply of oxidizing agent and efficient generation of nitrous acid, so that a low and constant hydrogen peroxide concentration in the solution can be maintained, the hydrogen peroxide concentration reacts with nitrous acid to generate peroxynitrous acid with extremely strong oxidizing property, the substance cooperates with hydroxyl radicals to realize precise and efficient degradation of hexamethylene diamine molecules, not only the biological toxicity of hexamethylene diamine is completely broken, the wastewater biodegradability is significantly improved, but also organic nitrogen is converted into nitrogen forms which are easy to be utilized by microorganisms, so that efficient and stable reaction conditions are created for subsequent anaerobic biological treatment, and finally the advantages of chemical oxidation and biological degradation are complemented.

A dual-responsive organic fluorescent compound, whose chemical name is (E)-1-(2-acetoxyethyl)-2-(2-(6-(diethylamino)-2,3-dihydro-1H-xanthene-4-yl)vinyl)-3,3-dimethyl-3H-indole-1-iodide, contains H + and ONOO ‑ The two response sites, whose emission colors can change with changes in molecular structure, can be used as fluorescent probes for detection and analysis in a variety of situations, including the absence of analytes, detection and analysis of hydrogen ion concentration alone or peroxynitrite anion concentration, or the coexistence of hydrogen ions and peroxynitrite anions. It can also be used to monitor changes in hydrogen ion concentration and peroxynitrite anion concentration in mitochondria of living cells and living organisms. The preparation method provided by the present invention is simple, has convenient raw material sources, and has a high yield. As a fluorescent probe, it has a wide range of applications, a sensitive response, a fast speed, and can simultaneously detect hydrogen ion concentration and peroxynitrite anion.

The present invention belongs to the technical field of fluorescent probes, and specifically relates to a fluorescent probe for detecting peroxynitrite ions, a preparation method thereof, and an application thereof. The fluorescent probe has the following general structural formula: wherein n is an integer between 1 and 4; R1, R2, and R3 are each independently selected from hydrogen, halogen, nitro, cyano, C 2‑6 The carboxylate group, C 1‑6 Alkyl, C 1‑6 Alkoxy, 1-3 halogen-substituted C 1‑6 Alkyl and C substituted by 1-3 halogen 1‑6 The fluorescent probe provided by the present invention can be used for the detection of peroxynitrite ions, and has the advantages of a wide linear range, fast response speed, high sensitivity, good selectivity, etc., and has good biocompatibility. It can be used to detect peroxynitrite ions in cells and organisms, and has high practical application value.

The invention discloses a peroxynitrite near-infrared fluorescent probe as well as a preparation method and application thereof, and belongs to the technical field of peroxynitrite near-infrared fluorescent probes. According to the technical scheme, the peroxynitrite near-infrared fluorescent probe is characterized in that the structural formula of the peroxynitrite near-infrared fluorescent probe is shown in the specification. The invention further specifically discloses a preparation method of the peroxynitrite near-infrared fluorescent probe and application of the peroxynitrite near-infrared fluorescent probe to selective detection of peroxynitrite in an aqueous solution, a biological cell system and animal liver tissue. The near-infrared fluorescent probe disclosed by the invention has the advantages of fluorescence emission in a near-infrared region, good selectivity, low detection limit and the like.

The present invention discloses a mass spectrometry imaging probe for detecting peroxynitrite, and its preparation method and application. The present invention provides a novel structural compound, the semicyanine structure of which has a positive center and can be used as a mass spectrometry detection label to capture signals; the tetrazine structure of the compound reacts with peroxynitrite to generate an oxadiazole structure, which can also be detected by mass spectrometry, and the response abundance is significantly higher than the response after reaction with other common reactive oxygen and reactive nitrogen species, showing excellent peroxynitrite selectivity, and can be used as a mass spectrometry probe for detecting peroxynitrite levels in biological samples. The probe can evaluate the production of peroxynitrite in in vivo samples by detecting the signal intensity of the corresponding product from the mass spectrometry channel, and quickly, simply and accurately analyze the spatial distribution of peroxynitrite in biological samples (such as liver tissue with ischemia-reperfusion injury) with the help of mass spectrometry imaging technology. The prior art has not disclosed the compound of the present invention, its preparation method and application.

The invention discloses a cyanine fluorescent compound as well as a preparation method and application thereof, and belongs to the technical field of fine chemical engineering. The structure of the cyanine fluorescent compound is shown as a formula (I), and the cyanine fluorescent compound can be used for detecting ONOO. The luminescent mechanism of the cyanine fluorescent compound is an intramolecular charge transfer (ICT) mechanism, the emission wavelength is 800nm, the cyanine fluorescent compound is connected with a diphenyl hypophosphite group, in the presence of peroxynitrite ONOO, the diphenyl hypophosphite group leaves through a high-selectivity addition-elimination way, the ICT effect is changed, the emission wavelength is blue-shifted to 633nm, and the cyanine fluorescent compound has the advantages that the fluorescence intensity of the cyanine fluorescent compound is improved; ratio-type fluorescence detection of ONOO is realized, and the detection limit reaches 0.57 mu M. (I)

The invention provides an application of activated water in promotion of crop growth, working gas is introduced into a plasma generator for reaction to obtain plasma gas, the plasma gas is introduced into a water body for treatment to obtain plasma activated water, and then the plasma activated water is subjected to light-shielding standing for 10-50 h to obtain the activated water, or the plasma gas and ozone are mixed for reaction and then are introduced into the water body for treatment, and the activated water is obtained. The activated water can effectively reduce the content of peroxynitrous acid, and can promote the growth of crops.

The invention discloses a fluorescent probe for rapidly and highly sensitively detecting peroxynitrite as well as a synthesis method and application of the fluorescent probe, and belongs to the technical field of chemical analysis and detection. The probe is obtained by condensing a halogen-substituted pyrone large pi system and a diphenyl phosphino compound, and has the following structure: a fluorophore of the probe is a halogen-substituted pyrone large pi system skeleton structure, and a response group to peroxynitrite is a diphenyl phosphino unit. The ultraviolet absorption peak of a single probe in a solution is located at-395nm, no obvious fluorescence is emitted, after the probe reacts with peroxynitrite, the ultraviolet absorption peak of the probe is subjected to red shift to-485nm, and strong fluorescence is emitted at-610nm. The probe molecule has the advantages of high speed, high selectivity, high sensitivity and the like in response to peroxynitrite, the reaction can be completed within 2 minutes, the concentration range of peroxynitrite detected in a solution is 0.03-10 [mu] mol.L <-1 >, and the detection limit is 8 nmol.L <-1 >. In addition, the probe can also be used for detecting peroxynitrite in cells.