Portable fluorescent cell analysis system and microscopic imaging method thereof

Abstract

The embodiment of the invention discloses a portable fluorescent cell analysis system and a microscopic imaging method thereof. The portable fluorescent cell analysis system comprises a microscopic imaging module, an objective table and a light source; the microscopic imaging module comprises an image acquisition unit and a microscopic amplification unit, and the effective working distance L1 between the image acquisition unit and the objective table meets the condition that L1 is larger than or equal to 30 mm and smaller than or equal to 100 mm in the direction perpendicular to the objectivetable; wherein the microscopic amplification unit at least comprises an amplification objective lens group, the amplification objective lens group comprises at least one objective lens, and the focallength f of the objective lens is greater than or equal to 2mm and less than or equal to 10mm; the light source comprises a bright field light source and at least one fluorescent light source. By theadoption of the technical scheme, it is guaranteed that fluorescence microscopic imaging can be achieved, it is guaranteed that the microscopic imaging module is small and compact by reasonably setting the effective working distance between the image acquisition unit and the objective table and reasonably setting the focal length of the objective lens, and the integration degree and portability ofthe portable fluorescence cell analysis system are improved.

Description

technical field [0001] The embodiments of the present invention relate to the technical field of fluorescence imaging, in particular to a portable fluorescence cell analysis system and a microscopic imaging method thereof. Background technique [0002] Microscopic imaging technology is currently being used more and more, widely used in teaching, scientific research, cell analysis and other fields, helping people realize the cognition of the microscopic field, and playing an increasingly important role. [0003] Fluorescence microscopy imaging is an important application of microscopy imaging technology. Fluorescence microscopy imaging uses an excitation light source of a specific wavelength to irradiate the sample, and the fluorescently labeled cell sample is excited to produce fluorescence of different wavelengths, and then the cells are observed under a fluorescence microscope. The morphology and structure of the sample are used to study the absorption, transportation, dis...

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Problems solved by technology

[0005] In view of this, the embodiment of the present invention provides a portable fluorescent cell analysis system and its microscopic imaging method to solve the technical problems of high cost, large volume and poor portability of the portable fluorescent cell analysis system in the prior art

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