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Rare earth nanoparticles with intense red fluorescence, preparation method of rare earth nanoparticles and application of rare earth nanoparticles to cell imaging

A nanoparticle and red fluorescent technology, applied in nanotechnology, nanotechnology, nano-optics, etc., can solve the problems of large size of rare earth fluorescent materials and difficulty in entering cells for imaging, and achieve low cost, good biocompatibility and biosafety Sexuality and low cytotoxicity

Active Publication Date: 2018-08-28
SOUTHEAST UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] For these rare earth fluorescent materials reported, the luminescence of rare earth ions mostly needs to be enhanced by organic ligands, and the preparation of organic ligands requires complex organic synthesis reactions; these rare earth fluorescent materials contain organic ligands, and many of them are insoluble in water, so they need to be used in applications. Organic solvents help solubilize; most of these rare earth fluorescent materials are large in size, and it is difficult to enter the interior of cells for cell imaging

Method used

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  • Rare earth nanoparticles with intense red fluorescence, preparation method of rare earth nanoparticles and application of rare earth nanoparticles to cell imaging
  • Rare earth nanoparticles with intense red fluorescence, preparation method of rare earth nanoparticles and application of rare earth nanoparticles to cell imaging
  • Rare earth nanoparticles with intense red fluorescence, preparation method of rare earth nanoparticles and application of rare earth nanoparticles to cell imaging

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1 The preparation of rare earth nanoparticles C:EuTb

[0022] Add 5 mL of polyethylene glycol 400 liquid, 0.25 mmol of europium nitrate solid and 0.25 mmol of terbium nitrate solid into the three-necked flask respectively to form a mixture with a molar ratio of europium ions: terbium ions of 55:1:1. Under an argon protective atmosphere, the mixture was heated to 100° C. and then continuously stirred for 30 minutes to form an emulsion. The temperature was gradually raised to 196° C. within 6 minutes and then the heating was stopped. The reaction solution was cooled to room temperature while stirring. The reaction solution was centrifuged, and the resulting yellow precipitate was washed twice with acetone and pure water by centrifugation, and finally dried at 60°C for use.

[0023] figure 1 is a transmission electron microscope image of the prepared rare earth nanoparticles C:EuTb, and C:EuTb is a spherical nanoparticle with a diameter of 3 to 5 nanometers. T...

Embodiment 2

[0024] The preparation of embodiment 2 rare earth nanoparticles C:EuTb

[0025] Add 5 mL of polyethylene glycol 400 liquid, 0.25 mmol of europium nitrate solid and 0.375 mmol of terbium nitrate solid into the three-necked flask respectively to form a mixture with a molar ratio of europium ions: terbium ions of 55:1:1.5. Under an argon protective atmosphere, the mixture was heated to 100° C. and then continuously stirred for 40 minutes to form an emulsion. The temperature was gradually raised to 196° C. within 6 minutes and then the heating was stopped. The reaction solution was cooled to room temperature while stirring. The reaction solution was centrifuged, and the resulting yellow precipitate was washed twice with acetone and pure water by centrifugation, and finally dried at 60°C for use.

[0026] The electron microscope image of rare earth nanoparticles C:EuTb prepared in this example is similar to Example 1, and the C:EuTb is a spherical nanoparticle with a diameter of 2-...

Embodiment 3

[0027] The preparation of embodiment 3 rare earth nanoparticles C:EuTb

[0028] Add 5 mL of polyethylene glycol 400 liquid, 0.5 mmol of europium nitrate solid and 0.625 mmol of terbium nitrate solid into the three-necked flask respectively to form a mixture with a molar ratio of europium ions: terbium ions of 55:2:2.5. Under an argon protective atmosphere, the mixture was heated to 100° C. and stirred continuously for 50 minutes to form an emulsion. The temperature was gradually raised to 196° C. within 6 minutes and then the heating was stopped. The reaction solution was cooled to room temperature while stirring. The reaction solution was centrifuged, and the resulting yellow precipitate was washed twice with acetone and pure water by centrifugation, and finally dried at 60°C for use.

[0029] The electron microscope image of the rare earth nanoparticles C:EuTb prepared in this example is similar to that of Example 1, and the C:EuTb is a spherical nanoparticle with a diameter...

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Abstract

The invention discloses rare earth nanoparticles with intense red fluorescence, a preparation method of the rare earth nanoparticles and an application of the rare earth nanoparticles to cell imaging.The rare earth nanoparticles comprise rare earth europium ions, terbium ions and carbon quantum dots, have the particle size being smaller than 5 nm, and emit characteristic fluorescence of the europium ions. According to the dual energy transfer effect of the carbon quantum dots and the terbium ions, the rare earth nanoparticles can glow strongly in an aqueous solution without organic ligands, so that the defects that a conventional fluorescence imaging material containing the organic ligands requires complex organic synthesis and needs an organic solvent to help solubilization due to poor water solubility are overcome. The rare earth nanoparticles are low in cytotoxicity, have good biocompatibility and safety and can be applied to fluorescent cell imaging.

Description

technical field [0001] The invention relates to a rare earth nanoparticle material, in particular to a rare earth nanoparticle with strong red fluorescence, a preparation method thereof and an application in cell imaging, belonging to the field of luminescent materials. Background technique [0002] Fluorescence imaging technology is widely used in biology, medical diagnostics and other fields. Fluorescence imaging technology can be used to observe and study the concentration changes and location migration of signaling molecules in cells, the drug transport rules and mechanism of action, and the interaction between functional nucleic acids and targets. The development of fluorescence imaging technology is of great significance to human health and safety. [0003] The fluorescence imaging methods that have been reported are: Chinese Patent Publication No. CN107200709A, 2017, Tang Benzhong, Hu Rongrong, Zang Qiguang, Zhao Zujin, Qin Anjun, a class of fluorescent compounds wit...

Claims

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Application Information

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IPC IPC(8): C09K11/65B82Y20/00B82Y40/00G01N21/64
CPCB82Y20/00B82Y40/00C09K11/7783G01N21/6486
Inventor 陈扬王路得
Owner SOUTHEAST UNIV
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