67results about How to "Hard to quench" patented technology

The invention discloses application of rhodamine B derivatives, and relates to application of rhodamine B derivatives provided with intra-molecular spiral rings and used as cell lysosome fluorescent colorants. The rhodamine B derivatives are rhodamine B derivatives with intra-molecular five-member rings; and the rhodamine B derivatives can be used as the cell lysosome fluorescent colorants and used for high-selectivity and long-term stably labeled cell lysosome. The application has the advantages that: (1) the amino-containing rhodamine B derivatives with intra-molecular five-member rings can be used as lysosome fluorescent labeling substances of cells of different types; (2) the rhodamine B derivatives have long retention time in cells and high light stability; and (3) the rhodamine B derivatives can be used for labeling and tracking intra-molecular lysosome for long time.

The invention discloses a method for detecting early apoptosis of cells. A phosphatidylserin antibody marked with a fluorescent dye Alexa Fluor 488 and a nucleic acid fluorescent dye 7-AAD are matched to detect the cells together, wherein the phosphatidylserin antibody is used for identifying normal living cells and apoptotic cells, and the nucleic acid fluorescent dye is used for identifying early apoptotic cells and late apoptotic cells in the apoptotic cells. After the two fluorescent dyes, namely the Alexa Fluor 488 and the 7-AAD with different emission wavelengths are detected and analyzed through a flow cytometry, the cells are divided into four subsets, wherein the Alexa Fluor 488+/7-AAD- subset is the early apoptotic cells.

The invention discloses a ruthenium (II) complex, of which the structure is as shown in the specification, and also discloses a preparation method of the complex. The method comprises the following steps: firstly, preparing X-RPIP from phenanthroline 5,6-diketone as a raw material, and then preparing a product, namely the ruthenium (II) complex from the X-RPIP and cis-Ru(L)2Cl2 as the raw materials; or firstly, preparing the X-RPIP from X-BrPIP as a raw material, and then preparing the product from the X-RPIP and cis-Ru(L)2Cl2 as the raw materials; or firstly, preparing Ru(L)2(X-R'''PIP)<2+> from Ru(L)2Cl2 and X-R'''PIP as the raw materials, and then preparing the product from the Ru(L)2(X-R'''PIP)<2+> as the raw material. The synthetic method disclosed by the invention is simple, especially the synthetic lambda-[Ru(bpy)2(p-BEPIP)]<2+> is free of toxicity on a living cell, quick to stride across a film, strong in fluorescence color, easy to observe, and not easy to quench, fluorescence excitation does not overlap with an emission waveband, and cell nucleus enrichment can be carried out.

The invention discloses a preparation method of a perovskite quantum dot composite, comprising the following steps: S1, mixing a first precursor and a second precursor with an organic solvent to obtain a perovskite precursor mixed liquid; S2, mixing the perovskite precursor mixed liquid in S1 with polymer microspheres to obtain a mixture of perovskite quantum dot polymer microspheres; S3, and subjecting the mixture of perovskite quantum dot polymer microspheres in S2 to solvent removal treatment to obtain the perovskite quantum dot composite. The preparation method of the perovskite quantum dot composite is simple, rapid, efficient and easy to operate, and can produce a perovskite quantum dot composite which is stable in air, is not easily quenched during light emitting, and can be furtherdirectly applied.

The invention discloses a tumor-targeted near-infrared fluorescent probe Met-IR-782 and a preparation method thereof. The fluorescent probe has strong fluorescence intensity, good stability, and highefficiency of photothermal conversion. Based on the fluorescent probe, a multi-modal nano contrast agent is prepared, the multi-modal nano contrast agent has a core-shell structure, Fe3O4 nanoparticles are used as a core, the surface of Fe3O4 nanoparticles is coated with Prussian blue as a shell, and Met-IR-782 is bonded to the Fe3O4 nanoparticles by an amide bond. The multi-modal nano contrast agent can realize multimodal angiography of MRI, fluorescence and photoacoustic imaging, and realizes comprehensive and accurate tumor diagnosis; by means of the photothermal properties of the Prussianblue and IR-782 and the thermomagnetic properties of the Fe3O4 nanoparticles, the multi-modal nano contrast agent can achieve thermomagnetic therapy and photothermal therapy of tumors.

The invention discloses a sensing material for concentration and temperature. The sensing material is an annular rare earth complex with a molecular formula of {[Ln(L1)3](L2O2)}2, wherein, Ln is a trivalent rare earth ion except for La<3+>, Gd<3+> and Lu<3+>, L1 is a beta-dione, and L2 is an aromatic conjugate compound. The sensing material for concentration and temperature can be reused for times, and the spectrum performance of the sensing material can steadily reappear.

The invention relates to a preparation method of fluorescence antibody for detecting a Newcastle disease virus and a solid-phase immunofluorescence detection assay kit. The preparation method comprises the following steps of: respectively deriving R-phycoerythrin (RPE) and an antibody resisting the Newcastle disease virus (NDV) by using a cross-linking agent SPDP (N-succinimidyl-3-(2-pyridyldithiol) propionate), cross-linking the derivatives in a proper molar ratio, and purifying through HPLC (High Performance Liquid Chromatography) to prepare an RPE marked NDV fluorescence antibody. The solid-phase immunofluorescence detection assay kit is formed from the fluorescence antibody, an NDV-resisting antibody, an agarose microsphere, diluted hydrochloric acid and a washing liquid. The assay kit comprises the following detection flows of: coating an activated microsphere with the antibody, washing, combining with a sample to be measured, washing, combining with the fluorescence antibody, fully washing, exciting by blue and green light in a fluorescence microscope, observing and judging the result. The prepared fluorescence antibody has the advantages of high yield, high purity, bright orange fluorescence and good stability; and the assay kit has signal enrichment action by using a spherical carrier and can increase detection sensitivity. The invention is applicable to the rapid detection of the Newcastle disease virus.

The invention discloses a preparing method for carboxylation porous crosslinked polystyrene copolymerized fluorescent microspheres. The preparing method is characterized by including the following steps of polystyrene seed sphere preparing, seed sphere activating, seed sphere swelling, polymerizing and pore-forming agent extracting. Compared with the prior art, the preparing method for the carboxylation porous crosslinked polystyrene copolymerized fluorescent microspheres has the advantages that the carboxylation porous crosslinked polystyrene copolymerized fluorescent microspheres with blue fluorescence, green fluorescence and red fluorescence are obtained; fluorochrome is evenly distributed in the microspheres, and therefore the high whole luminous efficiency of the microspheres is guaranteed; the fluorochrome stably exists in the microspheres, leakage and cancellation are not prone to occurrence, and pollution on test samples can be avoided; the grain size of the fluorescent microspheres prepared with the method is uniform, and is 7.5 microns, the specific area is large, and the good monodispersity is achieved; the light intensity is even in distribution; the preparing cost is low.

The invention discloses a preparation method of a near-ultraviolet polystyrene copolymerization fluorescence microsphere, and the preparation method is as follows: putting initiator azodiisobutyronitrile and dispersing agent polyvinylpyrrolidone in absolute ethanol to obtain a clear and transparent solution, simultaneously adding styrene and N-allyl carbazole to the solution for copolymerization reaction under the protection of nitrogen to obtain a polystyrene copolymerization fluorescence microsphere, and then introducing sulfonic acid groups or hydroxyl groups onto the surface of the polystyrene copolymerization fluorescence microsphere to produce the functional near-ultraviolet polystyrene copolymerization fluorescence microsphere. The preparation method adopts a method of dispersion polymerization to prepare the near-ultraviolet polystyrene copolymerization fluorescence microsphere having good monodispersity and stable fluorescent properties.

The invention relates to a method for preparing a fluorescent antibody for detecting avian influenza virus and a solid phase immunofluorescence detection kit. According to the invention, an avian influenza virus antibody is marked by allophycocyanin (APC) so as to prepare the avian influenza virus fluorescent antibody; the APC and the antibody are derived by a chemical cross-linking agent SPDP respectively; the derivatives are subjected to liquid phase cross-linking in a proper molar ratio; and then the fluorescent antibody is prepared by high pressure liquid chromatography purification. The solid phase immunofluorescence detection kit comprises the fluorescent antibody, CNBr activated agarose microspheres, the avian influenza virus antibody, cleaning solution and the like. The using method of the kit comprises the following steps of: activating a microsphere carrier and then coating the activated microsphere carrier by using the avian influenza virus antibody; cleaning; combining the microspheres coated with the antibody with a sample (an antigen) to be detected; combining the microspheres coated with the antibody with the fluorescent antibody after cleaning; removing the uncombined fluorescent antibody by cleaning; and observing under a fluorescence microscope and determining the result. The fluorescent antibody prepared by the method is characterized by high cross-linking efficiency, high purity, bright red fluorescence and stable performance. The microsphere solid phase carrier which is adopted by the kit can obviously improve fluorescent detection sensitivity, and is suitable for the quick detection of the avian influenza virus.

The invention discloses yellow fluorescent protein and application thereof. According to the invention, the wild-type red fluorescent protein DsRed2 is transformed so as to obtain the novel yellow fluorescent protein with amino acid mutation. The prokaryotic expression of the novel yellow fluorescent protein can achieve color development in a short time, is obvious in color development, is stablein color development in eukaryotic expression, is not easy to be quenched by incident light, and can be applied to fluorescent color development of various reporting systems and expression positioningof target proteins.

The invention discloses aggregation-induced fluorescent particles, a compound, multi-mode immunochromatography test paper and a preparation method of the multi-mode immunochromatography test paper. The aggregation-induced fluorescence particle is of a core-shell structure under a transmission electron microscope, has the particle size of 50-500nm, and is mainly composed of an aggregation-induced fluorescence material with color and fluorescence characteristics and a polymer. The aggregation-induced fluorescence particles are prepared by adopting a miniemulsion-solvent evaporation method, the equipment and the process are simple, the prepared composite particles are uniform in particle size, controllable in condition and good in repeatability, the concentration of the aggregation-induced fluorescence material in the particles is easy to control, and most importantly, the obtained particles have two characteristics of color and fluorescence. An immunochromatography test strip prepared by taking the particles as a signal carrier can be used for qualitative detection through naked eyes and can also be used for quantitative detection through fluorescence.

The invention relates to an amino-modified water-soluble fluorescent quantum dot and a preparation method thereof. The water-soluble fluorescent quantum dot is of a multi-layer core-shell structure, an oil-soluble fluorescent quantum dot (such as CdSe/ZnS) is adopted as a core, the surface of the oil-soluble fluorescent quantum dot is subjected to a silanization rection, so that the lipophilic and hydrophobic properties of the quantum dots are modified into hydrophily in one step; furthermore, an amino group (-NH2) is adopted, so that surface amino modification for the water-soluble quantum dot is finished; the prepared amino-modified water-soluble quantum dot has an average size of 10nm and is uniform in size, has a wide excitation spectrum continuously distributed in a range of 250-602nm, and has good emission spectrum monochromaticity with a maximum emission wavelength of 634nm and fluorescence-emission half peak width of 43nm. The amino-modified quantum dot is good in biocompatibility, can be coupled with various bioactive substances, and has great practical value and application prospect in the biology and medicine fields such as immunofluorescence diagnose, cell imaging and living imaging.

The invention relates to a fusarium moniliforme SiC quantum dot fluorescence labeling method and particularly relates to a fluorescence labeling method for fusarium moniliforme SiC in different growth stages by cytotoxicity-free aqueous phase silicon carbide quantum dots, and a long-time-period fluorescence imaging technology for living cells. The method comprises the following steps of preparing a mixed solution of slant strain fusarium moniliforme and a PD culture medium; preparing a mixed culture medium of a mycete liquid-SiC quantum dot aqueous phase solution from the mixed solution and an SiC quantum dot aqueous phase solution at a volume ratio of 3:1; performing constant-temperature shaking culture on the mixed culture medium at a temperature of 28 DEG C and a speed of 200r/min for 3-10 days, thereby obtaining a fusarium moniliforme SiC quantum dot fluorescent mark; applying the mark to seedling root dip dyeing, thereby realizing dynamic monitoring, tracing and fluorescence imaging of a process that the fusarium moniliforme infects plant roots. The method is simple and effective, is low in cost and is capable of realizing fluorescence labeling of the fusarium moniliforme in different periods.