44 results about "Influenza virus Antibody" patented technology

The invention relates to the biological technology field, in particular to a fluorescence micro cell agglutination method for detecting influenza virus antibody. The method in the invention comprises the following steps: hemagglutinin gene and neuraminidase gene of influenza virus are cloned and then are transfected to a eukaryotic cell; the hemagglutinin and neuraminidase are recombined and expressed on the surface of the eukaryotic cell; the recombinant expression cell is marked by fluorescence; the hemagglutinin and neuraminidase which are recombined and expressed on the surface of the cell are combined with hemagglutinin antibody and neuraminidase antibody of the influenza virus to carry out agglutination reaction; and pictures are taken by a fluorescence microscope, whether influenza virus antibody exists in a to-be-detected sample is judged according to whether the fluorescence area is increased. In the detecting method of the invention, the cell marked by fluorescence is used, the cell using amount is reduced, high-throughput detection can be carried out, and quick detection, early detection, on-spot detection and evaluation of the influenza virus antibody can be realized.

The present invention provides structural determinants important for binding to the stem domain of the HA protein of influenza virus, and methods of use thereof for production of high affinity neutralizing influenza virus antibodies based upon these determinants. The present invention further provides tools for determining the efficacy of an influenza virus vaccine. The present invention further provides a molecular signature useful for determining the efficacy of an influenza virus vaccine in a subject, or for predicting prior immunologic exposure or antigen responsiveness to vaccine or influenza virus infection.

The invention discloses a nanobody against H9N2 subtype avian influenza virus, a preparation method and an application. The amino acid sequence of the nanobody is shown in SEQ ID NO:1. The present invention expresses the nucleocapsid protein of H9N2 subtype avian influenza virus through prokaryotic expression technology, and then uses it as an immunogen to immunize Bactrian camels, constructs a phage library, and obtains an anti-H9N2 nucleocapsid strain by screening with phage display technology Protein nanobody, and the fusion expression of the nanobody and horseradish peroxidase (HRP), successfully prepared the fusion protein of the nanobody and HRP, and applied the fusion protein to the detection of anti-H9N2 subtype poultry in chicken serum Among influenza virus (AIV) antibodies, it was found that the detection method constructed by the fusion protein has high sensitivity, and has the advantages of simple operation, no need to use secondary antibodies, and short time-consuming detection of samples.

The invention relates to the technical field of biology, in particular to a method for detecting an influenza virus antibody through agglutination reaction based on an eukaryotic cell for recombinant expression on influenza virus hemagglutinin and neuraminidase. The method comprises the following steps of: taking the eukaryotic cell for the recombinant expression on the influenza virus hemagglutinin and the neuraminidase as an agglutination reaction matrix, and detecting the influenza virus hemagglutinin antibody and the neuraminidase antibody in a sample to be detected through the recombinant expression of the influenza virus hemagglutinin and the neuraminidase in the agglutination reaction way. In the invention, the influenza virus hemagglutinin and the neuraminidase are co-expressed on the surface of the eukaryotic cell, thereby effectively avoiding self-coagulation to affect the agglutination reaction due to the combination of the hemagglutinin and a cell receptor; at the same time, the invention can more widely and rapidly detect the influenza virus antibody in different serotypes.

Provided is an anti-influenza virus antibody that exhibits neutralizing activity beyond the barrier of the two groups of influenza viruses categorized according to the conservativeness of hemagglutinin amino acids, a method of producing the same, and a test method for determining whether the subject carries the neutralizing antibody.

The invention discloses an H1N1 influenza virus antibody and application thereof in ultra-micro detection of H1N1 viruses. The H1N1 influenza virus antibody comprises a heavy chain variable region, afirst heavy chain hypervariable region, a second heavy chain hypervariable region, a third heavy chain hypervariable region, a light chain variable region, a first light chain hypervariable region, asecond light chain hypervariable region and a third light chain hypervariable region. The H1N1 influenza virus antibody can be specifically combined with the H1N1 influenza viruses, and is used for ultra-micro detection of H1N1 influenza to achieve early prevention and treatment of the H1N1 influenza, thereby being of great economic and social significance.

The present invention provides structural determinants important for binding to the stem domain of the HA protein of influenza virus, and methods of use thereof for production of high affinity neutralizing influenza virus antibodies based upon these determinants. The present invention further provides tools for determining the efficacy of an influenza virus vaccine. The present invention further provides a molecular signature useful for determining the efficacy of an influenza virus vaccine in a subject, or for predicting prior immunologic exposure or antigen responsiveness to vaccine or influenza virus infection.

The invention relates to the biotechnology field, in particular to an influenza virus antibody. A light-chain variable region of the influenza virus antibody has an amino acid sequence shown as the SEQ ID No.1; a heavy-chain variable region of the influenza virus antibody has an amino acid sequence shown as the SEQ ID No.2. The antibody can well neutralize H3N2, H4N6 and H14N5 subtype influenza viruses, combine HA proteins of all subtype influenza viruses in a group 2, neutralize H3-subclade viruses and inhibit copying of the H3 subtype influenza viruses in a mouse body and has the important economic and social significance.

The invention first provides a preparation method of a swine H1N1 subtype influenza virus hemagglutinin (HA) recombinant protein. A pair of primers with HIS labels is designed by removing a signal peptide of the HA according to an HA sequence, and a prokaryotic expression plasmid with a 6*His-maltose-binding protein (MBP) combination label is constructed; the HA recombinant protein expressed in a soluble form is successfully obtained in an Escherichia coli expression system and can generate reaction with a mouse antibody with swine H1N1 subtype influenza virus HA, thereby showing that the HA recombinant protein has good antigenicity; a liquid phase chip detection technology is established by using the HA recombinant protein and is higher than ELISA (Enzyme-Linked Immuno Sorbent Assay) in sensitivity; the establishment of the method provides necessary technical supplement for detecting a swine influenza virus antibody, lays a technical basis for researching the liquid phase chip detection technologies of other epidemic disease antibodies, and provides a test reference for establishing multiple liquid phase chip detection technologies of multiple swine disease antibodies.

A stable liquid pharmaceutical preparation of an anti-influenza virus antibody and, more specifically, a stable liquid pharmaceutical preparation that comprises: (A) an anti-influenza virus antibody or a mixture of two or more different types of anti-influenza virus antibodies; (B) a surfactant; (C) a sugar or a sugar derivative; and (D) an amino acid. The stable liquid pharmaceutical preparation for an anti-influenza virus antibody disclosed herein has excellent storage stability at low temperature (5° C.), room temperature (25° C.), and high temperature (40° C.) and excellent long-term (12 months) storage stability, and may be administered intravenously, intramuscularly, transdermally, subcutaneously, intraperitoneally, topically, or in combinations thereof.

This invention disclose a kind of equipment used to detect the fowl influenza virus antibody correctly, fast, sensitively, with high specificity and economically. The equipment includes immunity sensor, enzyme labeled double antibody reaction system, electrolytic cell and data collection and process system; the immunity sensor includes the electrode system which is composed with the work electrode, the reference electrode and the assistant electrode which are printed in the insulated base plate; the electrolytic cell includes the detection base liquid, the beater and the temperature controller; the data collection and process system includes the electrochemistry workstation, the computer and the software system; the immunity sensor connects the electrochemistry workstation by lead; the work electrode of the immunity sensor is carbon electrode, which has coated the gold size induction film embed fowl influenza virus antibody. This invention also discloses a kind of method to use the equipment to detect the fowl influenza virus antibody.

This invention discloses a kind of equipment used to detect the fowl influenza virus antibody correctly, fast, sensitively, with high specificity and economically. The equipment includes immunity sensor, electrolytic cell and data collection and process system; the immunity sensor includes the electrode system which is composed with the work electrode, the reference electrode and the assistant electrode which are printed in the insulated base plate; the electrolytic cell includes the detection base liquid, the beater and the temperature controller; the data collection and process system includes the electrochemistry workstation, the computer and the software system; the immunity sensor connects the electrochemistry workstation by lead; the work electrode of the immunity sensor is carbon electrode, which has coated the gold size induction film embed fowl influenza virus antibody. This invention also discloses a kind of method to use the equipment to detect the fowl influenza virus antibody.

The invention provides a kind of influenza A virus IgA antibody immunofluorescence detection test strip and a preparation method thereof, wherein the influenza A virus IgA antibody immunofluorescence detection test strip comprises a sample pad, a marker pad, a nitrocellulose membrane, Absorbent paper and backboard; the nitrocellulose membrane includes the detection line of the influenza A virus NP protein antigen polypeptide fragment and the human IgA antibody control line; the marker pad is provided with an anti-human IgA antibody-fluorescent microsphere marker. The detection test paper provided by the invention has extremely high detection sensitivity, and the detection result can be obtained with the naked eye without resorting to an expensive PCR detector, thereby realizing rapid screening of influenza A virus. The invention also provides a detection method and application of the above-mentioned influenza A virus IgA antibody immunofluorescence detection test strip. The detection method has simple steps, easy operation, rapid results and is suitable for wide promotion.

The present invention provides a kind of influenza B virus IgA antibody immunofluorescence detection test strip and its preparation method, said type B influenza virus IgA antibody immune immunofluorescence detection test strip comprises a sample pad, a marker pad, a nitrocellulose membrane, Absorbent paper and backboard; the nitrocellulose membrane includes the detection line of the influenza B virus NP protein antigen polypeptide fragment and the human IgA antibody control line; the marker pad is provided with an anti-human IgA antibody-fluorescent microsphere marker. The detection test paper provided by the invention has extremely high detection sensitivity, and the detection result can be obtained with the naked eye without resorting to an expensive PCR detector, thereby realizing rapid screening of influenza B virus. The invention also provides a detection method and application of the above-mentioned influenza B virus IgA antibody immunofluorescence detection test strip. The detection method has simple steps, easy operation, rapid results and is suitable for wide promotion.

The invention relates to the field of biotechnology, particularly an influenza virus antibody detection method based on the eukaryotic cell agglutination reaction of recombined expression influenza virus hemagglutinin. In the detection method, influenza virus hemagglutinin genes are cloned and transfect eukaryotic cells, so that the surfaces of the eukaryotic cells express the influenza virus hemagglutinin protein in a recombined mode; the protein is combined with the influenza virus hemagglutinin antibody in the sample to be detected to produce agglutination reaction; and the influenza virus antibody in the sample to be detected is detected according to whether the agglutination reaction occurs. The detection method is quick and stable, and can be widely used for quickly detecting different serotypes of influenza virus antibodies.