Anti-H9N2 subtype avian influenza virus polypeptide and application thereof

An avian influenza virus, chicken and poultry technology, applied in the field of veterinary medical molecular immunology, can solve problems such as not involving H9N2 subtype influenza virus

Pending Publication Date: 2021-10-22
山东省健牧生物药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But it did not involve reports on the H9N2 subtype influenza virus

Method used

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  • Anti-H9N2 subtype avian influenza virus polypeptide and application thereof
  • Anti-H9N2 subtype avian influenza virus polypeptide and application thereof
  • Anti-H9N2 subtype avian influenza virus polypeptide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] A polypeptide against H9N2 subtype avian influenza virus, the amino acid sequence of the polypeptide is shown in SEQ ID NO: 1; the amino acid sequence: RRKKWLVFFVIFYFFR.

[0017] A nucleotide encoding the above antigen epitope polypeptide; the nucleotide sequence is SEQ ID NO: 2; nucleotide sequence: CGTCGTAAAAAATGGCTGGTTTTTTTTGTTATTTTTTATTTTTTTCGT.

[0018] A recombinant expression vector carrying the above-mentioned nucleotides.

Embodiment approach

[0022] 1 Materials and methods

[0023] 1.1 Main experimental materials and experimental animals H9N2 subtype avian influenza virus isolate (number: 402, referred to as H9N2AIV) is preserved by our laboratory; 10-day-old SPF chicken embryos are provided by Shandong Haotai Experimental Animal Breeding Co., Ltd.; FP4 polypeptide ( Amino acid sequence: RRKKWLVFFVIFYFFR), commissioned by BGI to synthesize.

[0024] The main reagents are penicillin and streptomycin double antibody solution (Shenggong).

[0025] Virus recovery and dilution H9N2 AIV isolates were thawed, inoculated to 10-day-old chicken embryos, cultured at 37°C for 3 days, collected allantoic fluid, and diluted to 200EID with normal saline 50 spare.

[0026] Preparation of test drugs

[0027] 1.4.1 Pre-experiment Accurately weigh 1 mg of FP4 polypeptide, dissolve it in 50 μL of ethanol, then add 950 μL of sterile water to dilute to 1 mg / mL stock solution, and dispense it. Mix 200 μL stock solution with 300 μL st...

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PUM

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Abstract

The invention belongs to the technical field of animal medical molecular immunology, and particularly relates to an anti-H9N2 subtype avian influenza virus polypeptide and an application thereof. The polypeptide for resisting the H9N2 subtype avian influenza virus has an amino acid sequence of RRKKWLVFFVIFYFFR, is applied to preparation of drugs for treating the avian influenza virus, and can also be combined with one or more of antiviral traditional Chinese medicines, interferon, influenza virus antibodies, transfer factors and the like to prepare products for resisting the H9N2 subtype avian influenza virus.

Description

technical field [0001] The invention belongs to the technical field of veterinary medicine molecular immunology, and in particular relates to a polypeptide against H9N2 subtype avian influenza virus and its application. Background technique [0002] Avian Influenza (AI) is a general term for avian infectious diseases caused by type A influenza virus that occurs in various poultry and wild birds. According to the antigenicity of hemagglutinin (HA) and neuraminidase (NA) on the surface of type A influenza virus particles, it is divided into many subtypes. According to the pathogenicity of clinical type A influenza virus, avian influenza can be divided into three types: high pathogenicity, low pathogenicity and non-pathogenicity. Although the H9N2 subtype avian influenza is a low-pathogenic avian influenza, it seriously endangers the production of the chicken industry and is an important reason for the high mortality rate of commercial broiler chickens. Secondary Escherichia ...

Claims

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Application Information

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IPC IPC(8): C07K7/08C12N15/11A61K38/10A61K39/145
CPCC07K7/08A61K39/145A61K38/00
Inventor 盛晓丹黄迪海秦卓明徐怀英郭卉秦春芝
Owner 山东省健牧生物药业有限公司
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