Device for detecting avian influenza virus antigen and detection method

A bird flu virus and antigen technology, applied in the direction of measuring devices, instruments, and material analysis through electromagnetic means, can solve the problems of long time, long detection time, complicated detection process, etc., and achieve the effect of low price and high specificity

Inactive Publication Date: 2012-10-31
ZHEJIANG GONGSHANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The agar immunodiffusion test method is convenient to obtain materials and easy to operate, but the detection time is long and the sensitivity is low; both immunofluorescence technology and ELISA require special equipment, the detection process is complicated, the cost is high, the detection time is long, and false positives are prone to occur; The detection process is complex and takes a long time, requiring high professional skills for operators, and the reagents used are harmful to human body and environment

Method used

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  • Device for detecting avian influenza virus antigen and detection method

Examples

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Embodiment 1

[0032] Example 1: The device for detecting avian influenza virus antigens of the present invention consists of an immunosensor, an electrolytic cell and a data acquisition and processing system.

[0033] The immunosensor includes a working electrode 2, a reference electrode 4 and an auxiliary electrode 3. Each electrode is printed on the rubber resin plate 1 by screen printing. The auxiliary electrode 3 is a graphite electrode, and the reference electrode 4 is an Ag / AgCl electrode. The working electrode 2 A carbon electrode is used as a supporting electrode, and a chitosan sensing membrane coated with horseradish peroxidase-labeled anti-H5N1 avian influenza virus antibody (HRP-anti-AIV) is coated on it.

[0034] The electrolytic cell includes a detection bottom liquid, a stirrer and a temperature controller, and the detection bottom liquid contains 0.4 mmol / LH 2 O 2 1mmol / L Thionine Acetate Buffer Solution, PH value is 6.5.

[0035] The data acquisition and processing system includes...

Embodiment 2

[0041] Example 2: Method for detecting avian influenza virus antigen

[0042] 1) Sample collection and processing

[0043] Take the dead poultry liver under aseptic conditions, put it into a sterile glass grinder, make a 100g / L suspension with PBS, add 1 / 10 volume of antibiotics, and move the processed sample into a centrifuge tube, 3000r / min Centrifuge for 10 minutes and take the supernatant as a sample. The samples were inactivated with 0.3-0.5% formalin before detection.

[0044] The collected or processed sample should be stored at 2℃~8℃ for no more than 24h; if long-term storage is required, it should be placed at -70℃, but repeated freezing and thawing (up to 3 times) should be avoided.

[0045] 2) Put the immunosensor described in Example 1 into the electrolytic cell, and the detection bottom liquid of the electrolytic cell contains 0.4mmol / L H 2 O 2 1mmol / L Thionine Acetate Buffer Solution, PH value is 6.5.

[0046] 3) Set the scan rate to 0.1V / s, apply a voltage to the workin...

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PUM

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Abstract

This invention discloses a kind of equipment used to detect the fowl influenza virus antibody correctly, fast, sensitively, with high specificity and economically. The equipment includes immunity sensor, electrolytic cell and data collection and process system; the immunity sensor includes the electrode system which is composed with the work electrode, the reference electrode and the assistant electrode which are printed in the insulated base plate; the electrolytic cell includes the detection base liquid, the beater and the temperature controller; the data collection and process system includes the electrochemistry workstation, the computer and the software system; the immunity sensor connects the electrochemistry workstation by lead; the work electrode of the immunity sensor is carbon electrode, which has coated the gold size induction film embed fowl influenza virus antibody. This invention also discloses a kind of method to use the equipment to detect the fowl influenza virus antibody.

Description

Technical field [0001] The invention relates to the technical field of rapid detection of avian influenza virus antigens, in particular to an electrochemical sensor and a detection method for detecting avian influenza virus antigens based on antigen-antibody reaction. Background technique [0002] Avian influenza virus (Avian Influenza virus, AIV) is a highly infectious virus that originates from poultry and can cause a large number of deaths of poultry and livestock in a very short period of time, and can cause death to humans. In recent years, many countries and regions have successively broken out highly pathogenic avian influenza, which not only caused huge economic losses to related industries, but also seriously threatened human health and safety. [0003] Rapid avian influenza detection is one of the most critical measures for disease diagnosis, early monitoring, preventing the spread of the epidemic and ensuring food safety. [0004] At present, the detection of avian influe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/569G01N33/558G01N27/416
Inventor 赵广英吴淑春
Owner ZHEJIANG GONGSHANG UNIVERSITY
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