18 results about "Bactrian camel" patented technology

The invention discloses double-milk-source fermented milk of double-peak camel milk and brown milk and a preparation method of the double-milk-source fermented milk, and the preparation method comprises the following steps: mixing raw materials: mixing the double-peak camel milk and the brown milk according to a mass ratio to obtain double-milk-source mixed milk; pretreatment: sequentially carrying out homogenization treatment and heat treatment on the double-milk-source mixed milk; strain inoculation: inoculating the pretreated double-milk-source mixed milk with a fermentation strain, wherein the fermentation strain comprises a core strain and an auxiliary strain; the core strains are lactobacillus and streptococcus thermophilus which have tolerance to antibacterial components of the camel milk, and the core strains contain strains capable of producing exopolysaccharides. Through scientific matching of lactobacillus, streptococcus thermophilus and specific auxiliary strains which have tolerance to antibacterial components of the camel milk, the inhibition effect of natural antibacterial components such as lysozyme and lactoferrin in the camel milk on conventional strains is successfully overcome.

The application discloses a hemoglobin camel-derived nanobody and a preparation method and application thereof. The hemoglobin camel-derived nanobody is an amino acid sequence shown in SEQ ID NO: 7, is a hemoglobin camel-derived nanobody specific to hemoglobin derived from a bactrian camel, has small molecules, can easily penetrate, has high specificity, good stability, is easy to produce and transform, has wide application prospects, and can be applied to the fields of biomedical research, clinical diagnosis and treatment and the like.

The invention provides a C3-2 strain for producing bactrian camel chymosin and application of the C3-2 strain, and belongs to the technical field of biological engineering. According to the present invention, the Latin of the C3-2 strain is Kluyveromyces lactis, and is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: M20251316. According to the method, the C3-2 strain is used for fermentation, the fermentation liquor is concentrated by adopting an ultrafiltration method, small molecular impurities are removed, and the bactrian camel chymosin with high enzyme activity is obtained by combining affinity chromatography. The cheese prepared from the bactrian camel chymosin obtained by the preparation method disclosed by the invention is high in crosslinking degree, harder and finer in texture, smaller, more uniform and more compact in microstructure texture, lower in bitter taste and higher in overall volatile flavor substance content, and the overall evaluation is superior to that of cheese prepared from commercial chymosin.

The present application relates to a kind of polypeptide NbM14 identifiable and neutralizing MERS-CoV, including 3 complementarity determining regions CDR1-3, sequence as SEQ ID NO:1-3 show.The present application carries out nanobody drug development to MERS-CoV, by preparing MERS-CoV S protein, immunizing bactrian camel, utilizes phage library display nanomantibody platform technology etc., screen to the nanobody VHH specific binding MERS-CoV, identify its CDR sequence, and construct humanized VHH-huFc1;Simultaneously, using pseudovirus neutralization experiment evaluates the curative effect of NbM14 in the treatment of MERS-CoV infection, provides potential detection agent and therapeutic drug for the prevention and treatment of MERS-CoV infection.

The invention provides application of phytosterol ester in preparation of a product for improving lactation performance of bactrian camels, and belongs to the field of bactrian camel breeding. It is found that the lactation performance of the bactrian camels can be remarkably improved by adding the phytosterol ester, especially the beta-sitosterol ester, in the feed, the milk yield of the bactrian camels is increased, and the camel milk fat content and the camel milk density are increased. The invention further provides a feed for improving the lactation performance of the bactrian camels and a method for improving the lactation performance of the bactrian camels, the milk yield of the bactrian camels can be effectively increased, and the quality of camel milk is improved.

The invention belongs to the technical field of biology, and particularly relates to a bactrian camel clostridium perfringens AD type bivalent inactivated vaccine as well as a preparation method and application thereof. The invention relates to a bactrian camel clostridium perfringens AD type bivalent inactivated vaccine, the bivalent inactivated vaccine comprises two inactivated strain antigens, and the antigens are a bactrian camel A type aerogenic bacteria clostridium perfringens strain with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.35194 and a double hump D type aerogenic bacteria clostridium perfringens strain with the preservation number of CGMCC No.35195. According to the application, a newly found bactrian camel clostridium perfringens strain is used as an antigen. The 1059 ST type clostridium perfringens are separated from intestinal tracts of young bactrian camels for the first time, and the 1059 type (A type) and 675 type (D type) clostridium perfringens are inactivated to prepare the bivalent inactivated vaccine.

The application discloses an anti-ENR nanobody, a coding gene, a cELISA detection method and purposes, and belongs to the technical field of biology. The amino acid sequence of the anti-ENR nanobody is shown as SEQ ID NO. 1, and the nucleic acid sequence for coding the anti-ENR nanobody is shown as SEQ ID NO. 2. A new type of competitive ELISA (cELISA) is established by screening the specific nanobody against ENR from an immunized bactrian camel library. When detecting the enrofloxacin residue in animal-derived food, the method has high sensitivity and specificity, can detect the ENR residue as low as 6.49 ng / mL in the sample, and has no cross reaction with other antibiotics. It is shown that the cELISA established in the application is a simple, rapid, sensitive and low-cost immunodetection method, and has great application prospect in the detection of ENR residue in animal-derived food.

The present invention relates to a polypeptide NbM9 that can recognize and neutralize MERS-CoV, comprising three complementary determining regions (CDR1-3), the sequences of which are shown in SEQ ID NOs: 1-3. The present invention develops nanoantibody drugs targeting MERS-CoV. By preparing the MERS-CoV S protein, immunizing Bactrian camels, and utilizing a phage library platform technology to display nanomonoclonal antibodies, a nanoantibody VHH that specifically binds to MERS-CoV was screened, its CDR sequence was identified, and a humanized VHH-huFc1 was constructed. Simultaneously, a pseudovirus neutralization experiment was used to evaluate the efficacy of NbM9 in treating MERS-CoV infection, providing a potential detection agent and therapeutic drug for the prevention and treatment of MERS-CoV infection.

The present invention discloses a nano-antibody for detecting aflatoxin B1 and its application, belonging to the field of food detection. The amino acid sequence of the nano-antibody is shown in SEQ ID NO: 1. The present invention constructs a Bactrian camel immune antibody library, uses phage display technology to solid-phase-coat the detection antigen on an ELISA plate, and then puts the Bactrian camel immune antibody library into the plate for affinity panning to obtain a nano-antibody that specifically binds to aflatoxin B1. The nano-antibody is then applied to the immunological detection and analysis of aflatoxin B1, and a rapid, sensitive, and stable method for detecting aflatoxin B1 is established through this immunoassay. The present invention provides a new method for the specific and rapid detection of aflatoxin B1 in the food field.

The invention provides a separation method and an identification method of bactrian camel testis interstitial cells, and belongs to the technical field of cell separation. The method comprises the following steps: mixing 0.08-0.12% of collagenase IV solution and 0.23-0.27% of trypsin-EDTA (Ethylene Diamine Tetraacetic Acid) according to a volume ratio of 1: 2, digesting a bactrian camel testis tissue block, then carrying out hypotonic treatment purification to remove redundant germ cells, carrying out differential adhesion purification to remove a large amount of supporting cells, carrying out percoll density gradient centrifugation purification to remove a small amount of supporting cells, and carrying out freeze drying to obtain a finished product. And transferring the suspension with the percoll gradient of 34-60% into a new culture bottle to obtain the interstitial cells with the purity of more than or equal to 95%, and completing identification by combining morphological observation, immunofluorescence staining and secretion function detection. The separated bactrian camel testis interstitial cells can be used for double-hump reproductive performance research, and a foundation is laid for the reproductive performance of bactrian camels.

The application belongs to the technical field of biology, and particularly relates to a bactrian camel milk production trait related gene EVX2 and application thereof as a molecular marker. The application uses a second-generation sequencing technology to perform genome resequencing on 162 bactrian camels, performs whole genome association analysis on camel non-fat solid rate traits, and identifies a molecular marker related to high / low bactrian camel milk non-fat solid rate in an EVX2 gene. The marker is located at a 48754135 bp site on chromosome 5, a C>A single base mutation (g.48754135C>A) occurs at the mutation site, the mutation site can improve the non-fat solid rate of camel milk, and the marker can be used to carry out molecular marker assisted selection on the bactrian camel non-fat solid rate trait.

The application provides a method for establishing a Bactrian camel intestinal tract organoid model and application thereof, and the method is as follows: mucous mucus of Bactrian camel intestinal tract tissue is scraped off, washed, digested, washed again, crypt cells are scraped, cells are suspended in a culture medium, screened, filtrate is collected, centrifuged, supernatant is discarded, cells are resuspended in a DMEM / F12 culture medium containing bovine serum albumin to obtain a cell suspension; matrix glue is added to the cell suspension, mixed uniformly, inoculated, grown, grown culture medium is added, and then the Bactrian camel intestinal tract organoid model is obtained; and the obtained Bactrian camel intestinal tract organoid model is subcultured. The application also provides application of the Bactrian camel intestinal tract organoid model constructed by the construction method, which is used for in-vitro model construction of Bactrian camel intestinal tract related drugs or / and biological products. The construction method can efficiently obtain the Bactrian camel intestinal tract organoid which has self-organization and self-renewal capabilities and highly simulates intestinal epithelial tissue, and realizes stable subculture.

The application discloses a medicine injection method for killing camel head fly larvae parasitizing in the nasal cavity of a bactrian camel. A pair of non-bony structures similar to a triangle and the size of a thumb are arranged at the joint of nasal bone, maxillary bone and frontal bone. The pair of non-bony structures are at the same horizontal level with the eye socket of the bactrian camel, are located at the intersection of the inner corner of the eye and the upper edge of the eye socket, and are away from the eye socket by 4-5 cm. After a syringe is vertically inserted into 2-3 cm at the site, the needle of the syringe can reach the medial part of the concha nasalis. After the site is inserted into 2.5-3 cm in the clockwise direction and is inclined by 45 degrees, the needle can reach the lateral part of the concha nasalis. The medicine injection method can directly inject the insect killing medicine to the parasitizing position of the first instar larvae, and the purpose of killing the first instar larvae is achieved. The medicine injection method provides a new method for killing the first instar larvae in the overwintering period, and can reduce the economic loss of the camel raising industry caused by the nasal fly maggot disease.

The invention provides recombinant camel chymosin and application thereof, and belongs to the technical field of gene recombination. The amino acid sequences of the recombinant camel chymosin disclosed by the invention are as shown in SEQ ID NO. 1 to SEQ ID NO. 5. The physicochemical properties, the amino acid composition and the structure of the bovine chymosin and the bactrian camel chymosin are compared and analyzed by utilizing a bioinformatics method. Analyzing a three-dimensional conformation of an enzyme-substrate compound through molecular docking, and identifying key residues influencing the catalytic efficiency and the protein stability by combining charge analysis, flexible analysis and alanine scanning; rosetta is used for site-specific saturation mutation library construction, and preliminary screening of mutants is realized through energy minimization and conformation enumeration; based on quantum mechanics / molecular mechanics simulation and machine learning prediction, the catalytic efficiency change rule of the mutant is quantified, and the optimal bactrian camel chymosin mutant can be screened.

The invention provides a separation method and an identification method of bactrian camel testis sertoli cells, and belongs to the technical field of cell separation. The method comprises the following steps: mixing 0.08-0.12% of collagenase IV solution and 0.23-0.27% of trypsin-EDTA (Ethylene Diamine Tetraacetic Acid) according to a volume ratio of 1: 1, digesting a bactrian camel testis tissue block, and then combining differential adhesion and hypotonic treatment to obtain bactrian camel testis supporting cells with the purity of 95% or above, meanwhile, reducing cell damage, and enabling the cell survival rate to exceed 85%; and the identification is completed by combining morphological observation, immunofluorescence staining and secretion function detection. The separated bactrian camel testicular sertoli cells can be used for double-hump reproductive performance research, and a foundation is laid for the reproductive performance of bactrian camels.

This invention provides a method for isolating and identifying interstitial cells from Bactrian camels, belonging to the field of cell isolation technology. A mixture of 0.08–0.12% collagenase IV solution and 0.23–0.27% trypsin-EDTA at a volume ratio of 1:2 is used to digest Bactrian camel testicular tissue blocks. The mixture is then purified by hypotonic treatment to remove excess germ cells, differential adhesion purification to remove a large number of supporting cells, and Percoll density gradient centrifugation to remove a small number of supporting cells. The suspension, obtained from a 34%–60% Percoll gradient, is transferred to a new culture flask to obtain interstitial cells with a purity ≥95%. Identification is performed using morphological observation, immunofluorescence staining, and secretory function testing. The interstitial cells isolated from Bactrian camels using this invention can be used for research on the reproductive performance of Bactrian camels, laying the foundation for understanding the reproductive performance of Bactrian camels.

The application provides a bactrian camel feed and a production method thereof, and the bactrian camel feed is composed of the following components in parts by weight: cottonseed meal 7-8 parts, soybean meal 7-8.5 parts, corn 23-27 parts, corn alcohol dregs 9-10 parts, corn gluten meal 3.5-5.5 parts, wheat flour 5.5-6.5 parts, corn germ meal 14-15 parts, wheat bran 9-10 parts, corn white skin 3.5-5.5 parts, palm fatty acid powder 3-4 parts, white sugar 1-1.5 parts, salt 1.5-2.5 parts, premix 0.2-0.4 parts. The bactrian camel feed of the application takes soybean meal and cottonseed meal as the protein source, provides multiple protein sources, has more comprehensive nutritional value, and supplements palm fatty acid powder in a targeted manner, improves the energy level, and further improves the milk yield of bactrian camels. The preparation method is simple, the application provides a special feed for bactrian camel breeding industry, which is comprehensive in nutrition, controllable in cost, and can significantly improve the milk production performance.

The application belongs to the technical field of biology, and particularly relates to a bactrian camel milk fat rate related gene CARD11 and application thereof as a molecular marker. The application discloses a bactrian camel milk production trait related gene CARD11, and a breeding method and application of using a single nucleotide polymorphism site (SNP) of the gene as a molecular marker for assisted selection. The CARD11 gene and a site related to high / low milk fat rate in a bactrian camel lactation trait are found for the first time, which provides a candidate gene for breeding a bactrian camel variety with excellent traits and improving camel milk quality. The application uses a second-generation sequencing technology to perform genome resequencing on 162 bactrian camels, performs whole genome association analysis on camel milk fat rate traits, and identifies a molecular marker related to the bactrian camel milk fat rate in the CARD11 gene. The marker is located at a 9786961bp site on chromosome 18, a C>T single base mutation (g.9786961C>T) occurs at the site, the mutation site can improve the camel milk fat rate, and the marker can be used for carrying out molecular marker assisted selection on the bactrian camel milk fat rate trait.