9 results about "Bactrian camel" patented technology

The application discloses a hemoglobin camel-derived nanobody and a preparation method and application thereof. The hemoglobin camel-derived nanobody is an amino acid sequence shown in SEQ ID NO: 7, is a hemoglobin camel-derived nanobody specific to hemoglobin derived from a bactrian camel, has small molecules, can easily penetrate, has high specificity, good stability, is easy to produce and transform, has wide application prospects, and can be applied to the fields of biomedical research, clinical diagnosis and treatment and the like.

The invention provides a C3-2 strain for producing bactrian camel chymosin and application of the C3-2 strain, and belongs to the technical field of biological engineering. According to the present invention, the Latin of the C3-2 strain is Kluyveromyces lactis, and is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: M20251316. According to the method, the C3-2 strain is used for fermentation, the fermentation liquor is concentrated by adopting an ultrafiltration method, small molecular impurities are removed, and the bactrian camel chymosin with high enzyme activity is obtained by combining affinity chromatography. The cheese prepared from the bactrian camel chymosin obtained by the preparation method disclosed by the invention is high in crosslinking degree, harder and finer in texture, smaller, more uniform and more compact in microstructure texture, lower in bitter taste and higher in overall volatile flavor substance content, and the overall evaluation is superior to that of cheese prepared from commercial chymosin.

The invention belongs to the technical field of biology, and particularly relates to a bactrian camel clostridium perfringens AD type bivalent inactivated vaccine as well as a preparation method and application thereof. The invention relates to a bactrian camel clostridium perfringens AD type bivalent inactivated vaccine, the bivalent inactivated vaccine comprises two inactivated strain antigens, and the antigens are a bactrian camel A type aerogenic bacteria clostridium perfringens strain with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.35194 and a double hump D type aerogenic bacteria clostridium perfringens strain with the preservation number of CGMCC No.35195. According to the application, a newly found bactrian camel clostridium perfringens strain is used as an antigen. The 1059 ST type clostridium perfringens are separated from intestinal tracts of young bactrian camels for the first time, and the 1059 type (A type) and 675 type (D type) clostridium perfringens are inactivated to prepare the bivalent inactivated vaccine.

The application belongs to the technical field of biology, and particularly relates to a bactrian camel milk production trait related gene EVX2 and application thereof as a molecular marker. The application uses a second-generation sequencing technology to perform genome resequencing on 162 bactrian camels, performs whole genome association analysis on camel non-fat solid rate traits, and identifies a molecular marker related to high / low bactrian camel milk non-fat solid rate in an EVX2 gene. The marker is located at a 48754135 bp site on chromosome 5, a C>A single base mutation (g.48754135C>A) occurs at the mutation site, the mutation site can improve the non-fat solid rate of camel milk, and the marker can be used to carry out molecular marker assisted selection on the bactrian camel non-fat solid rate trait.

The application provides a method for establishing a Bactrian camel intestinal tract organoid model and application thereof, and the method is as follows: mucous mucus of Bactrian camel intestinal tract tissue is scraped off, washed, digested, washed again, crypt cells are scraped, cells are suspended in a culture medium, screened, filtrate is collected, centrifuged, supernatant is discarded, cells are resuspended in a DMEM / F12 culture medium containing bovine serum albumin to obtain a cell suspension; matrix glue is added to the cell suspension, mixed uniformly, inoculated, grown, grown culture medium is added, and then the Bactrian camel intestinal tract organoid model is obtained; and the obtained Bactrian camel intestinal tract organoid model is subcultured. The application also provides application of the Bactrian camel intestinal tract organoid model constructed by the construction method, which is used for in-vitro model construction of Bactrian camel intestinal tract related drugs or / and biological products. The construction method can efficiently obtain the Bactrian camel intestinal tract organoid which has self-organization and self-renewal capabilities and highly simulates intestinal epithelial tissue, and realizes stable subculture.

The invention provides recombinant camel chymosin and application thereof, and belongs to the technical field of gene recombination. The amino acid sequences of the recombinant camel chymosin disclosed by the invention are as shown in SEQ ID NO. 1 to SEQ ID NO. 5. The physicochemical properties, the amino acid composition and the structure of the bovine chymosin and the bactrian camel chymosin are compared and analyzed by utilizing a bioinformatics method. Analyzing a three-dimensional conformation of an enzyme-substrate compound through molecular docking, and identifying key residues influencing the catalytic efficiency and the protein stability by combining charge analysis, flexible analysis and alanine scanning; rosetta is used for site-specific saturation mutation library construction, and preliminary screening of mutants is realized through energy minimization and conformation enumeration; based on quantum mechanics / molecular mechanics simulation and machine learning prediction, the catalytic efficiency change rule of the mutant is quantified, and the optimal bactrian camel chymosin mutant can be screened.

This invention provides a method for isolating and identifying interstitial cells from Bactrian camels, belonging to the field of cell isolation technology. A mixture of 0.08–0.12% collagenase IV solution and 0.23–0.27% trypsin-EDTA at a volume ratio of 1:2 is used to digest Bactrian camel testicular tissue blocks. The mixture is then purified by hypotonic treatment to remove excess germ cells, differential adhesion purification to remove a large number of supporting cells, and Percoll density gradient centrifugation to remove a small number of supporting cells. The suspension, obtained from a 34%–60% Percoll gradient, is transferred to a new culture flask to obtain interstitial cells with a purity ≥95%. Identification is performed using morphological observation, immunofluorescence staining, and secretory function testing. The interstitial cells isolated from Bactrian camels using this invention can be used for research on the reproductive performance of Bactrian camels, laying the foundation for understanding the reproductive performance of Bactrian camels.

The application provides a bactrian camel feed and a production method thereof, and the bactrian camel feed is composed of the following components in parts by weight: cottonseed meal 7-8 parts, soybean meal 7-8.5 parts, corn 23-27 parts, corn alcohol dregs 9-10 parts, corn gluten meal 3.5-5.5 parts, wheat flour 5.5-6.5 parts, corn germ meal 14-15 parts, wheat bran 9-10 parts, corn white skin 3.5-5.5 parts, palm fatty acid powder 3-4 parts, white sugar 1-1.5 parts, salt 1.5-2.5 parts, premix 0.2-0.4 parts. The bactrian camel feed of the application takes soybean meal and cottonseed meal as the protein source, provides multiple protein sources, has more comprehensive nutritional value, and supplements palm fatty acid powder in a targeted manner, improves the energy level, and further improves the milk yield of bactrian camels. The preparation method is simple, the application provides a special feed for bactrian camel breeding industry, which is comprehensive in nutrition, controllable in cost, and can significantly improve the milk production performance.

The application belongs to the technical field of biology, and particularly relates to a bactrian camel milk fat rate related gene CARD11 and application thereof as a molecular marker. The application discloses a bactrian camel milk production trait related gene CARD11, and a breeding method and application of using a single nucleotide polymorphism site (SNP) of the gene as a molecular marker for assisted selection. The CARD11 gene and a site related to high / low milk fat rate in a bactrian camel lactation trait are found for the first time, which provides a candidate gene for breeding a bactrian camel variety with excellent traits and improving camel milk quality. The application uses a second-generation sequencing technology to perform genome resequencing on 162 bactrian camels, performs whole genome association analysis on camel milk fat rate traits, and identifies a molecular marker related to the bactrian camel milk fat rate in the CARD11 gene. The marker is located at a 9786961bp site on chromosome 18, a C>T single base mutation (g.9786961C>T) occurs at the site, the mutation site can improve the camel milk fat rate, and the marker can be used for carrying out molecular marker assisted selection on the bactrian camel milk fat rate trait.