35 results about "Bactrian camel" patented technology

The invention relates to a marking method for indicating and distinguishing domestic and wild Bactrian camel DNA microsatellites in the technical field of bio-engineering. The steps of the method are as follows: ear tissue samples of Bactrian camels are collected, and genomic DNA is extracted according to the conventional phenol / chloroform method; PCR amplification is carried out on the genomic DNA of the Bactrian camels by means of using 16 pairs of designed primers; PCR products are separated by means of using non-denatured polyacrylamide gel electrophoresis, and genotype is classified according to the lengths of the PCR products; distribution of different microsatellite-marked genotypes in the Bactrian camels is compared, and the result shows that allele-specific genes of 3 microsatellite loci (VOLP-10, YWLL36 and YWLL38) exist between domestic and wild Bactrian camels; allele-specific amplified products are cloned and sequenced. The method can be operated simply and can distinguish the domestic Bactrian camels and the wild Bactrian camels at the genome molecular level, thereby providing an important means and method for researching the heredity and the evolution of the Bactrian camels, especially the identification and the protection of the wild Bactrian camels.

The present invention provides a two-humped camel-based anti-goat pox virus VHH-4-1 and a use thereof, and belongs to the field of biotechnology. The anti-goat pox virus VHH-4-1 is prepared by VHH-4-1screening, expressing and purifying, functionalized labeling and activity verifying, anti-goat pox virus concentrating and purifying, optimizing various indicators of a kit, etc. Due to the fact thatthe provided kit uses the functionalizedly labeled VHH-4-1, the kit avoids steps of primary antibodies, secondary antibodies, anti-antibodies, etc., improves sensitivity and stability, shortens time,saves resources and breaks through drawbacks that traditional Elisa methods are time-consuming, laborious, etc. The provided anti-goat pox virus VHH-4-1 provides a basis for establishing sensitive goat pox virus clinical diagnosis method and studying a virus infection mechanism, and also provides a key marker for the developed goat pox virus antibody solid phase competition Elisa kit. In addition, goat pox attenuated vaccine is used to be subjected to cell enlarge cultivation, concentration and purification as a coating antigen to improves the sensitivity.

The invention relates to an optimal immune procedure and method of an immune camel. The immune method comprises the step of multipoint subcutaneous injecting at the back of a Bactrian camel, wherein the immune period is two weeks, the injected antigen dosage is 1mg / ml, and the immune is carried out three times. The invention further relates to a preparation flow and a method of a rabbit anti-camel IgG enzyme-labeled secondary antibody, a method for detecting camel anti-serum titer by applying the rabbit anti-camel IgG enzyme-labeled secondary antibody through ELISA and an optimal detection condition.

The invention discloses an immunological kit for detecting florfenicol. A florfenicol immunodetection antibody is prepared by immunizing a Camelidae animal with a conjugate of a florfenicol derivativeand a carrier protein as a florfenicol complete antigen. The high-affinity florfenicol Camelidae animal antibody is obtained in the invention, the alpaca polyclonal antibody titer of florfenicol is 1:32000, and the Bactrian camel polyclonal antibody titer of florfenicol is 1:256000; and a florfenicol enzyme-linked immunoassay kit can be developed in the invention, the florfenicol detection limitof the kit is 0.3 ng / mL, the half-inhibitory concentration is 13.3 ng / mL, and the kit can achieve high-flux, fast and highly-sensitive florfenicol detection requirements. The kit can be used for the residual detection of florfenicol in livestock and poultry, aquatic products and other animal foods, has the advantages of easiness in operation, high in sensitivity and low price, is very suitable forscreening a large number of samples, and has wide application prospects.

The invention discloses a bactrian camel-based anti-goat pox virus VHH-4-2 and application thereof, and belongs to the field of biotechnology. The technical scheme includes: screening, expressing, purifying, functional labeling and activity verifying of anti-goat pox virus VHH-4-2, concentrating and purifying of goat pox virus, optimizing of various indicators of a kit, etc. The functionally labeled VHH-4-2 is used in the invention, thus avoiding operation steps of primary antibody, secondary antibody, anti-antibody, etc., improving sensitivity and stability, shortening time and saving resources. The bactrian camel-based anti-goat pox virus VHH-4-2 breaks through shortcomings that the traditional Elisa method is time-consuming, laborious, etc. The bactrian camel-based anti-goat pox virus VHH-4-2 lays a foundation for establishing a clinical diagnosis method for sensitive goat pox virus and for studying the infection mechanism of the virus; and the bactrian camel-based anti-goat pox virus VHH-4-2 also provides a key marker for the development of a goat pox virus antibody-immobilized competitive Elisa kit with complete proprietary intellectual property rights. In addition, the attenuated goat pox vaccine is used as a coating antigen after expanded culture, concentration and purification, so that the sensitivity is improved.

The invention relates to an Alxa bactrian camel superovulation method, and belongs to the technical field of animal production. According to the technical scheme, the implementation schedule, the medicine ingredients and the medicine dosage are determined, an Alxa bactrian camel superovulation procedure is set up, the blank that no bactrian camel superovulation procedure exists in China is filled in, estrus synchronization of animals is achieved, each experimental animal reacts to the superovulation procedure, and 7 to 22 functional corpora lutea can be formed. The medicine and the procedure are more suitable for bactrian camels and more economical compared with a foreign dromedary superovulation procedure.

The invention discloses a bactrian camel heavy-chain (HC) variable-domain antibody resisting a porcine circovirus 2 as well as a preparation method and an application thereof, and belongs to the field of a biotechnology. A PCV2 (Porcine Circovirus Virus) inactivated vaccine immunized bactrian camel is firstly utilized and a phage display technology is utilized to establish a PCV2 immune VHH antibody base; and a high-specificity variable domain antibody of heavy chains of HCAbs (VHH) which has a good affinity with the PCV, particularly a PCV2 virus antigen; and the antibody disclosed by the invention has an amino acid sequence shown as SEQ (sequence) ID NO.1 or an amino acid sequence which is shown as SEQ ID NO.2 and has the sequence isogeny being more than 95%. The PCV2 heavy-chain variable-domain antibody disclosed by the invention has the very important meanings on researching porcine circovirus, particularly researching a porcine circovirus 2-type infection mechanism and establishing rapid antigen detection with high sensitivity and specificity or diagnosing a reagent.

The invention relates to a heat clearing and detoxifying Guangchang white lotus seed electuary and a production method thereof. Guangchang white lotus seed, deer gall, Loveyly Hemsleya Root, bactrian camel gallstone, Folium Polygoni Tinctrorii, seed of Japanese apricot, India Canna Rhizome, shrubalthea leaf, Hoya carnosa, honeysuckle flower and Indigofera trifoliate are adopted as main raw materials. The production method mainly comprises the steps of material selecting, drying, crushing, granulation and packaging to form a finished product. The heat clearing and detoxifying Guangchang white lotus seed electuary has the advantages of health, heat clearing and detoxifying effects, fast effectiveness, pure naturalness, and no toxic or side effects, has a good auxiliary treatment effect on main symptoms of wind heat colds, and is suitable for household eating.

The invention discloses a single-domain heavy chain antibody for detecting porcine epidemic diarrhea virus, a preparation method and application. The present invention adopts porcine epidemic diarrhea virus to immunize Alxa Bactrian camels, uses its peripheral blood lymphocytes to establish a single-domain heavy chain antibody library against porcine epidemic diarrhea virus, and couples the expressed and purified porcine epidemic diarrhea virus S protein to As an antigen on the enzyme plate plate, the single domain heavy chain antibody library was screened immunologically by using phage display technology, and the single domain heavy chain antibody gene against the S protein of porcine epidemic diarrhea virus was obtained, and the single domain heavy chain antibody gene was transferred into the large intestine In Bacillus, a single-domain heavy-chain antibody strain highly expressed in Escherichia coli was established, and the gene sequence of each single-domain heavy-chain antibody strain was analyzed to obtain the amino acid of the VHH chain of the single-domain heavy-chain antibody against the S protein of porcine epidemic diarrhea virus Sequence SEQ ID NO.8. The antibody of the invention has good stability and specificity, and can be used for detecting and treating PEDV.

The application provides a tamde virus, the virus strain is TAMV / XJ01 / Xinjiang / China / 2018, and the deposit number of the virus strain is CGMCC No.17585; the virus strain is from the tick subtype of Xinjiang Bactrian camel It is isolated from Hyalomma asiaticum; the application also provides primers and probes for detecting the virus, which have guiding significance for the mechanism of TAMV virus infection in ticks and the monitoring and control of the virus.