Influenza B virus IGA antibody immunofluorescence detection test strip and its preparation method, detection method and application

A technology for influenza B virus and immunofluorescence detection, applied in the field of medical testing, can solve the problems of low sensitivity, high price, and inability to popularize at the grassroots level.

Active Publication Date: 2020-04-24
GUANGZHOU RHFAY BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since this detection technology requires an expensive PCR detector and a certified laboratory with certain detection conditions, it cannot be promoted at the grassroots level
Antigen colloidal gold immunization technology directly collects throat swabs of patients, and can judge the patient's infection with influenza virus by naked eyes; but it has the disadvantages of low sensitivity and easy to cause missed detection

Method used

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  • Influenza B virus IGA antibody immunofluorescence detection test strip and its preparation method, detection method and application
  • Influenza B virus IGA antibody immunofluorescence detection test strip and its preparation method, detection method and application
  • Influenza B virus IGA antibody immunofluorescence detection test strip and its preparation method, detection method and application

Examples

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Embodiment 1

[0061] Example 1: Influenza B virus IgA antibody immunofluorescence detection test strip and preparation method (capture method)

[0062] Such as figure 1 As shown, the influenza B virus IgA antibody immunofluorescence detection test strip of this embodiment includes: a back plate 5, a sample pad 1, a marking pad 2, a nitrocellulose membrane 3, and an absorbent paper 4. Among them, the absorbent paper 4, the nitrocellulose membrane 3, the marking pad 2, and the sample 1 pad are adhered to the back plate 5 in sequence.

[0063] The nitrocellulose membrane 3 includes the detection line T of the influenza B virus NP protein antigen polypeptide fragment and the control line C of the human IgA antibody. The amino acid sequence of the influenza B virus NP protein antigen polypeptide fragment is shown in the sequence table Seq ID NO.1.

[0064] The labeling pad 2 is provided with anti-human IgA antibody-fluorescent microsphere markers. The anti-human IgA antibody is selected from goat an...

Embodiment 2

[0077] Example 2: Preparation of test strips for immunofluorescence detection of another influenza B virus IgA antibody (indirect method)

[0078] Such as figure 1 As shown, the influenza B virus IgA antibody immunofluorescence detection test strip of this embodiment includes: a back plate 5, a sample pad 1, a marking pad 2, a nitrocellulose membrane 3, and an absorbent paper 4. Among them, the absorbent paper 4, the nitrocellulose membrane 3, the marking pad 2, and the sample 1 pad are adhered to the back plate 5 in sequence.

[0079] The nitrocellulose membrane 3 includes a detection line T for anti-human IgA antibody and a control line C for influenza B virus NP protein antibody. The anti-human IgA antibody is selected from goat anti-human IgA polyclonal antibody, mouse anti-human IgA monoclonal antibody, rabbit anti-human IgA monoclonal antibody or rabbit anti-human IgA polyclonal antibody. Influenza B virus NP protein antibody is selected from mouse anti-influenza B virus NP...

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Abstract

The present invention provides a kind of influenza B virus IgA antibody immunofluorescence detection test strip and its preparation method, said type B influenza virus IgA antibody immune immunofluorescence detection test strip comprises a sample pad, a marker pad, a nitrocellulose membrane, Absorbent paper and backboard; the nitrocellulose membrane includes the detection line of the influenza B virus NP protein antigen polypeptide fragment and the human IgA antibody control line; the marker pad is provided with an anti-human IgA antibody-fluorescent microsphere marker. The detection test paper provided by the invention has extremely high detection sensitivity, and the detection result can be obtained with the naked eye without resorting to an expensive PCR detector, thereby realizing rapid screening of influenza B virus. The invention also provides a detection method and application of the above-mentioned influenza B virus IgA antibody immunofluorescence detection test strip. The detection method has simple steps, easy operation, rapid results and is suitable for wide promotion.

Description

Technical field [0001] The invention belongs to the field of medical testing, and relates to an influenza virus detection test strip, in particular to an influenza B virus IgA antibody immunofluorescence detection test strip, and a preparation method, detection method and application thereof. Background technique [0002] Seasonal influenza viruses are prevalent every year around the world. In the population, seasonal influenza is mainly composed of influenza A and B viruses. Influenza B is a highly contagious and fast spreading disease. It is characterized by sudden onset, chills, fever, body temperature rising to a peak within a few hours to 24 hours, and gastrointestinal symptoms are more common than other types of influenza. It is mainly spread through droplets in the air, contact between people, or contact with contaminated items. The antigenic variation of influenza B virus is very slow. There are two strains with distinct antigenicity and genetic characteristics in infl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68
CPCG01N33/68
Inventor 游爱萍李有生刘辉刘轶锴
Owner GUANGZHOU RHFAY BIOTECH CO LTD
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