6results about How to "Extensive testing" patented technology

The present invention belongs to the technical field of pharmacological and toxicological analysis of small molecule compounds, and specifically discloses a method for single-molecule manipulation detection of in vitro drug metabolizing enzyme-catalyzed DNA adduct formation, comprising the steps of: a) fixing a double-stranded DNA molecule between a fixed surface and a movable surface; b) mechanical stretching to measure the length of the double-stranded DNA molecule; c) applying a reaction solution containing a small molecule compound and a drug metabolizing enzyme component, and reacting; d) washing away the small molecule compound and the drug metabolizing enzyme component; e) mechanical stretching to detect the change in the length of the double-stranded DNA, thereby judging whether the small molecule compound forms an embedded adduct with DNA based on the change in length, or judging the amount and / or reaction kinetics of the embedded adduct formed by the small molecule compound and DNA. The present invention uses a single-molecule mechanical manipulation method to detect the amount and reaction kinetics of the embedded adduct formed by the drug metabolizing enzyme-catalyzed small molecule compound and DNA.

The present invention provides an industrial plastic barrel pressure test device, which relates to the technical field of pressure testing, and includes a test frame, a clamping assembly, a transmission mechanism and a boosting mechanism. Side baffles are welded on both sides of the test frame, and the clamping assembly includes a first clamping plate and a second clamping plate, and the bottoms of the first clamping plate and the second clamping plate are both integrally formed with sliders, and the surface of the second clamping plate is provided with a groove, and the interior of the groove is embedded with a transmission mechanism. The test device indirectly acts the pressure applied by the boosting mechanism on the top of the industrial plastic barrel to be tested through the transmission mechanism, so as to simulate a scenario where the pressure range and area are not fixed, and multiple test tasks with different pressure ranges and areas can be quickly switched, which improves the test efficiency, and can also perform boosting tests on the sides of the industrial plastic barrel, and simulate the pressure data that multiple industrial plastic barrels can withstand after being stacked after being horizontally laid down, so that the test is more comprehensive and more accurate.

A CAN bus feature fusion intrusion detection method based on adversarial learning relates to a CAN bus intrusion detection method, and time features and signal features of the same CAN ID are spliced into a comprehensive feature matrix for data processing and adversarial training of a detection model; each LSTM sub-network of the detection model is used for processing the corresponding CAN ID comprehensive characteristic matrix; the outputs of the LSTM sub-networks are spliced into a joint potential vector; a coding and decoding re-coding model of the detection model carries out dimension reduction on the joint potential vector to obtain an original intermediate representation, then dimension raising reconstruction is carried out to obtain a reconstructed comprehensive feature close to the latest comprehensive feature, and then dimension reduction is carried out to obtain a reconstructed intermediate representation; and according to the reconstruction error and the recoding error, carrying out abnormal scoring on the latest comprehensive characteristics to judge whether an attack behavior exists or not. According to the method, wider attack types can be detected, the method can still be effectively operated under the condition of lacking labeled training data, and the detection efficiency and safety are remarkably improved.

The utility model discloses a low power circuit integration junction box, including casing, modular interface array, embedded control module and safety protection module, wherein, the modular interface array contains at least three kinds of specifications test terminal, and each test terminal is connected with the measured circuit board through the elastic contact, embedded control module contains microprocessor, programmable signal generator and AD conversion unit. In addition, the low power circuit integration junction box still includes power module and wireless communication module. The utility model provides junction box through using S3C2451 embedded control processor to realize the automatic measurement and judgement of low power point fast, and can save the various measurement parameters of detected through programmable signal generator and AD conversion unit, then through wireless communication module carries out data transmission, and the convenient implementation detection result inquiry and transmission have improved the detection efficiency, and the detection result also has higher measurement accuracy and good repeatability.

The invention relates to the technical field of ultrasonic honing, in particular to a full-automatic spherical precise ultrasonic honing method and system based on resonant frequency simulation, and the method comprises the steps: receiving an ultrasonic honing instruction, starting an ultrasonic honing system, defining motion constraint, free constraint and excitation conditions, setting a honing transducer, a honing tool rod and a spherical honing head, and establishing a simulation model. The method comprises the steps of setting a simulation range and a honing workpiece, executing modal analysis to obtain a simulation modal set, extracting a resonant frequency set and a resonant vibration mode set, drawing a modal heat map, screening an optimal frequency mode, scanning feature points of the workpiece, moving the honing workpiece to a honing position and calculating the offset of the workpiece, executing precise ultrasonic honing and detecting the real-time offset. And the real-time offset is adjusted, the shape error is calculated, the adjusting pressure is calculated based on the shape error, and full-automatic spherical precise ultrasonic honing is completed. The automatic degree and honing precision of full-automatic spherical ultrasonic honing can be improved.

The invention discloses a method for simultaneously determining PAGln and PAGly in plasma based on an ultra-high performance liquid chromatography triple quadrupole tandem mass spectrometer, and belongs to the technical field of clinical biological sample detection. The method comprises the following steps: 1) uniformly mixing a quality control sample and a plasma sample with a precipitation reagent respectively, fully extracting, centrifuging, and taking supernate, namely a sample to be detected; (2) detecting the sample to be detected by using an ultra-high performance liquid chromatography triple quadrupole tandem mass spectrometer, wherein the chromatographic conditions are as follows: a chromatographic column is a C18 chromatographic column; carrying out gradient elution by adopting the mobile phase A and the mobile phase B; and 3) measuring the peak areas of PAGln and PAGly in the sample to be detected, and respectively substituting the peak areas into the standard curve for calculation to obtain the contents of PAGln and PAGly in the sample to be detected, the two metabolites PAGln and PAGly can be detected at the same time, and the method has the advantages of being high in accuracy, sensitivity and specificity, small in needed sample size, simple in sample pretreatment and the like.