5results about How to "Wide linear detection range" patented technology

The application belongs to the technical field of sensing, and discloses a preparation method of a high-stability wearable lactic acid biosensor based on Prussian blue analogues. The method comprises the following steps: (1) constructing a three-electrode sensor array on a flexible substrate through a screen printing technology; (2) sequentially depositing gold nanoparticles and a PB-NiHCF composite catalytic layer on the surface of a working electrode through a cyclic voltammetry method; (3) finally obtaining the sensor by curing under the condition of 4 DEG C and avoiding light after layer-by-layer modification of a chitosan-carbon nanotube conductive layer, lactic acid oxidase and a diffusion-limiting membrane. The sensor significantly improves the stability under physiological pH conditions through the synergistic catalytic effect of PB-NiHCF, the linear detection range reaches 30 mM, the sensitivity is 223.94 nA mM ‑1 cm ‑2 , and the sensor is suitable for real-time monitoring of sweat lactic acid.

This invention proposes a microneedle electrochemical biosensor and its fabrication method. The sensor comprises: a substrate and a glucose sensing electrode, a sodium penicillin sensing electrode, a sodium chloride sensing electrode, a reference electrode, and a counter electrode disposed on the substrate. Specifically: the glucose sensing electrode comprises a first graphite layer, a Prussian blue layer, and an enzyme immobilization layer stacked from bottom to top; the sodium penicillin sensing electrode comprises a second graphite layer and a β-lactamase immobilization layer stacked from bottom to top; and the sodium chloride sensing electrode comprises a third graphite layer, a signal conversion layer, and an ion recognition membrane layer stacked from bottom to top. This sensor selectively measures the concentrations of glucose, sodium penicillin, and sodium chloride, achieving high sensitivity and accuracy in detection.

The invention discloses a double-plasma metal synergistic metal organic framework composite SERS (Surface Enhanced Raman Scattering) substrate as well as a preparation method and application thereof in diazepam detection, and belongs to the technical field of drug detection. The SERS substrate is Ag (at) ZIF-8 (at) Au, and is specifically prepared by the following steps: mixing an Ag (at) ZIF-8 solution and an AuNPs solution, violently stirring, then standing for reaction, and obtaining the Ag (at) ZIF-8 (at) Au after the reaction is finished. The SERS substrate has good sensitivity, uniformity, reproducibility and storage stability and can be used for sensitive and efficient detection of diazepam, the detection limit is as low as 0.126 ng / mL, and the detection linear range is wide and reaches 10 <-2 >-10 <-9 > g / mL.

The application belongs to the technical field of metal organic framework (MOF) immobilized enzyme, and particularly relates to a method for wrapping enzyme by seed orientation and application thereof. The application discloses a method for synthesizing enzyme@MOF composite by seed orientation, and based on the characteristics of MOF crystal growth, the seed orientation synthesis method is innovatively proposed. The addition of seed can greatly promote the synthesis efficiency of enzyme@MOF composite and greatly increase the encapsulation efficiency of enzyme. Meanwhile, the seed orientation synthesis method has no requirement on the chemical properties of enzyme / protein, and does not need to destructively modify the enzyme / protein to improve the encapsulation efficiency, so that the seed orientation synthesis method has good universality. The seed orientation synthesis method solves the problems of low universality and low efficiency in the existing enzyme@MOF composite synthesis method, and has an expected application prospect in the fields of biological catalysis, biological sensing, biological medicine and the like.

The present application relates to the technical field of molecular detection and diagnosis, and particularly relates to a tetrahedral probe, a preparation method thereof and application of the tetrahedral probe in detection of apurinic / apyrimidinic endonuclease 1 (APE1). The DNA tetrahedral probe is formed by self-assembly of a frame DNA chain, a triple helix complex and a hairpin H chain. The frame DNA chains interact to form a stable tetrahedral skeleton; when APE1 exists in a sample, it can specifically recognize and cut apurinic / apyrimidinic sites on the triple helix complex and the hairpin H chain, trigger a cascade reaction and realize signal amplification; nucleic acid drugs in the probe are released with the cutting, and thus the synergy of detection and treatment is realized. The technical solution can solve the technical problem of unsatisfactory sensitivity and accuracy of in vitro detection of APE1 enzyme in the prior art, has low detection limit and wide linear range. The technical solution has important clinical application value and scientific research significance, and is expected to promote the development of non-small cell lung cancer diagnosis and treatment technology.