20 results about "Strain specificity" patented technology

The invention discloses a primer composition and application of the primer composition in detection of animal bifidobacterium subsp. Lactis HN019. According to the present invention, bifidobacterium animalis subsp. Lactis HN019 is adopted as an object, bacterial strain specific genetic markers are screened through whole genome sequencing and single nucleotide polymorphism analysis, and a fluorescent quantitative PCR technology is combined so as to obtain the efficient, specific and stable HN019 quantitative detection primer composition and the method thereof; through system verification, the method is suitable for an HN019 strain level detection technical framework in scientific research and industrial application scenes, and also provides key technical support for market supervision and industrial healthy development of probiotic foods.

The application belongs to the technical field of biological medicine, and particularly relates to application of an anti-invasive fungal infection drug in anti-mycobacterium infection. The Fosmanogepix in the application has obvious strain specificity for mycobacterium, can effectively inhibit the activity of a standard strain of Kansas mycobacterium and a clinically isolated strain of Kansas mycobacterium, the MIC of the standard strain of Kansas mycobacterium (ATCC12478) is 1 ug / mL, and the MIC of the clinically isolated strain of Kansas mycobacterium is 2 ug / mL. 50 The compound can be applied to development of an anti-Kansas mycobacterium infection drug as a candidate drug, and has important significance for preventing and treating Kansas mycobacterium disease, especially drug-resistant Kansas mycobacterium disease.

The invention discloses a strain specific culture medium prediction method, a medium and computer equipment. The method comprises the following steps: acquiring strain gene function annotation data and encoding the data into a high-dimensional feature vector; performing principal component analysis dimension reduction on the high-dimensional feature vector; obtaining candidate culture medium component information and encoding the candidate culture medium component information into a binary vector; splicing the low-dimensional strain feature representation and the medium component vector to form a joint input feature vector; inputting the joint input feature vector into a pre-trained deep residual network model; and outputting a growth compatibility probability score and sorting the candidate culture media. By integrating high-throughput genome annotation data and a known culture medium formula, a data-driven intelligent prediction model is constructed and is used for efficiently recommending an optimal culture medium composition suitable for a specific microbial strain, and the prediction precision and the screening speed of microbial isolated culture are remarkably improved.

The invention discloses early-maturing and high-yield morchella septimelata and a molecular marker thereof. The preservation number of the morchella septimelata is CCTCC (China Center For Type Culture Collection) NO: M 20252848. The whole anti-season planting period of the strain provided by the invention is 50-60 days, and is shortened by about 20 days compared with that of a conventional septimelate variety; the whole period of in-season cultivation (sowing in 11-December) is 60-90 days and is shortened by about one month compared with that of a conventional sextelate variety, fruiting can be achieved before the Spring Festival, and the high-temperature risk in spring is effectively avoided; the yield is improved by more than 50% compared with that of a conventional variety, and white mold infection is not easily caused. The invention also develops a strain specific InDel marker and a primer, which can be used for variety authenticity identification.

The invention belongs to the technical field of yeast strains and fermentation, and discloses a yeast S19k and application thereof. The strain is hansenula polymorpha S19K. According to the method, the exclusive strain S19k of the red wine is screened, and a sequential inoculation process is combined, so that the limitation of the prior art in the aspects of aroma complexity, color stability and environmental tolerance is broken through, and a differentiated solution is provided for producing area personalization of the domestic red wine. According to the invention, a traditional single enzyme activity screening mode is broken through, a comprehensive screening system of the bacterial strain and the enzyme produced by the bacterial strain in a gradient wine brewing stress environment is constructed, and the functional stability of the bacterial strain in a real brewing environment is ensured. Compared with existing patents, the strain specificity, the metabolism regulation and control mechanism and the industrial adaptability are remarkably innovated, and higher market competitiveness and technical barriers are achieved.

The invention discloses a rapid bacterial identification method based on thermally driven death-melting curve analysis, and belongs to the technical field of microbiological detection. According to the method, a death-melting curve of bacterium / strain specificity is constructed by utilizing membrane thermal stability difference of bacteria and genome dsDNA sequence difference, and Pearson correlation analysis is performed on the death-melting curves of different bacteria by using an SPSSPRO data analysis platform, so that bacteria variety differentiation and rapid identification of the bacteria are realized. The identification method provided by the invention has the characteristics of rapid and efficient detection, simple operation, low cost, high identification accuracy, high specificity and strong universality.

This invention relates to the field of microbial technology, specifically disclosing a strain of *Lactobacillus paracasei* BL23 that promotes iron absorption and its applications. The preservation number of *Lactobacillus paracasei* BL23 is CGMCC No. 32468. Experimental verification shows that this strain has the ability to reduce ferric iron to ferrous iron, and that specific reducing epitopes exist on the cell surface, enabling it to secrete siderophores. This strain utilizes siderophore secretion and Fe... 3＋ It reduces iron absorption through a dual mechanism and has strong strain specificity, enabling it to independently improve iron deficiency anemia without relying on exogenous iron supplements, thus providing a novel probiotic resource for the prevention and treatment of iron deficiency anemia.

The invention belongs to the technical field of biological application, and particularly relates to an antibacterial peptide and application thereof, and the amino acid sequence of the antibacterial peptide is shown as SEQ ID NO.1. The invention provides an antibacterial molecule with a novel structure, the antibacterial molecule is composed of 28 amino acids and has no significant homology with reported mussel antibacterial peptide sequences such as Mytilin and Myticin, the antibacterial peptide enriches an antibacterial peptide molecular library, the theoretical isoelectric point of the polypeptide is up to 11.72, the net charge is + 4, the polypeptide has high positive charge density and a hydrophobic amino acid proportion of 53.6%, and the antibacterial peptide has a good application prospect. The amphiphilic structural characteristics are obviously better than those of the traditional antibacterial peptide, and the antibacterial peptide is endowed with the capability of efficiently targeting a bacterial membrane. Bacteriostatic experiments prove that the inhibition rate on escherichia coli reaches 60%, the inhibition rate on staphylococcus aureus is 20%, obvious concentration dependence and strain specificity are shown, the effect is accurate, and broad-spectrum drug resistance is not likely to be induced.

The invention relates to the technical field of flexible materials, in particular to a preparation method of a wireless passive biaxial strain sensor based on a nested double-loop antenna. The strain sensor designed and manufactured by the method is composed of nested double-ring antennas, and two decoupled resonant frequencies are generated by regulating and controlling capacitance and inductance characteristics of an inner ring structure and an outer ring structure under a stretching condition and are respectively sensitive to x-axis and y-axis strain specificity. The preparation of the stretchable biaxial strain sensor is realized by activating printing ink composed of two self-healing materials of liquid metal and styrene-ethylene / butylene-styrene copolymer (SEBS) through laser scanning. Due to the circuit-free design, crosstalk-free bidirectional strain monitoring is realized, and a new scheme is provided for the biomechanical sensor.

The present invention relates to the technical field of detection of Pleurotus maxima strains, and discloses an SSR marker fingerprint of Pleurotus maxima PG46 strain, its construction method, and application. The fingerprint consists of 10 pairs of SSR molecular markers developed based on whole-genome sequencing of Pleurotus maxima. The construction method includes: mycelium culture; genomic DNA extraction; SSR molecular marker detection; polyacrylamide gel electrophoresis detection; and application includes: amplifying SSR molecular markers of Pleurotus maxima strains, comparing the obtained band pattern with the fingerprint pattern, and the strain consistent with the fingerprint pattern is Pleurotus maxima PG46 strain. Compared with conventional morphological identification, antagonism test, and fruiting experiment, the present invention has the advantages of short detection time, high accuracy, and good repeatability. Using the SSR fingerprint constructed by the present invention, PG46 has strain specificity among these 7 different strains.

The invention relates to the technical field of bioinformatics, in particular to a pathogenic microorganism genome, a construction method, a computer system and application. According to the method, a genome data quality control strategy in the pathogenic microorganism genome database creation process is innovated, ANI and AF double indexes are creatively adopted for classification error recognition, contig pollution sequence recognition and k-mer short sequence pollution recognition are combined for sequence pollution recognition and processing on genome data, the number of genomes is reserved to the maximum, and the number of the genomes is reduced to the maximum. According to the method, contig level sequence errors are accurately recognized, strain specific sequences are prevented from being mistakenly deleted, the diversity of species genomes is guaranteed, and the constructed database can improve the identification accuracy / sensitivity of pathogen infected species.

The present disclosure relates to a recombinant chimeric virus-like particle (cVLP) vaccine composition against rotavirus infection. The vaccine comprises a virus-like particle fragment including a hepatitis B core (HBc) protein functioning as an adjuvant, into which a heterologous immunogenic domain derived from the rotavirus VPS* protein is inserted within the major immunodominant region (MIR) of HBc. The construct is encoded by SEQ ID NO. 1, and expressed in a prokaryotic system. The cVLPVPS* vaccine composition of the present disclosure offers a non-replicating, protein-based alternative to live attenuated vaccines, and the platform may help address challenges related to safety, strain specificity, and antigen delivery.

The invention discloses a bifidobacterium animalis subsp. Lactis BD108 preparation as well as application and a product of the bifidobacterium animalis subsp. Lactis BD108 preparation in emotion regulation, and belongs to the technical field of microorganisms. The invention aims to solve the technical problems that the survival rate of viable bacteria is low after oral administration, the mood improvement effect is unstable and long-acting intervention is difficult to realize due to the problems of weak strain specificity, insufficient mood regulation activity, poor preparation protection effect, single action pathway and the like existing in the conventional probiotic strain in mood regulation application. According to the key point of the technical scheme, the freeze-dried microsphere preparation of the animal bifidobacterium subsp. Lactis BD108 is provided and comprises the animal bifidobacterium subsp. Lactis BD108 and a composite carrier solution, the preservation number of the animal bifidobacterium subsp. Lactis BD108 is GDMCC (China General Microbiological Culture Collection Center) No.67826; the composite carrier solution is composed of trehalose, pullulan, whey protein phosphopeptides and a solvent.

The present disclosure relates to a recombinant chimeric virus-like particle (cVLP) vaccine composition against rotavirus infection. The vaccine comprises a virus-like particle fragment including a hepatitis B core (HBc) protein functioning as an adjuvant, into which a heterologous immunogenic domain derived from the rotavirus VPS* protein is inserted within the major immunodominant region (MIR) of HBc. The construct is encoded by SEQ ID NO. 1, and expressed in a prokaryotic system. The cVLPVPS* vaccine composition of the present disclosure offers a non-replicating, protein-based alternative to live attenuated vaccines, and the platform may help address challenges related to safety, strain specificity, and antigen delivery.

The invention discloses an information encryption method and system based on strain genome coding and identification. The method comprises the following steps: extracting target gene sequence features from a strain genome database, including conservative marker gene sequences, necessary functional gene sequences and genome variation site distribution, and generating a strain specific key matrix; after to-be-encrypted information is converted into binary codes, a dynamic mapping rule is established according to the strain specificity key matrix, the binary codes are converted into quaternary pseudo-gene sequences, and strain identity identification codes are embedded into the quaternary pseudo-gene sequences; a hierarchical coding mechanism is adopted to allocate different security levels according to strain system development levels, and multi-level key management and authority control are achieved; inserting a strain specific marker sequence and an integrity check code into the pseudo-gene sequence to generate a final biological ciphertext; according to the method, high-security quantum resistant encryption is realized by utilizing natural diversity and randomness of microbial genomes.

This application provides a method and apparatus for mining strain-specific fragments, relating to the field of bioinformatics technology. The method includes: comparing the gene similarity of a target strain with multiple strains of the same species, and identifying the strain with the highest gene similarity as a reference strain; performing collinear alignment of the genomes of the target strain and the reference strain to identify and extract genomically differential fragments present in the target strain but not in the reference strain; segmenting the genomically differential fragments into multiple candidate fragments; identifying candidate fragments that meet preset conditions as specific standard fragments; and merging multiple specific standard fragments that are adjacent or overlapping to generate a specific fragment for the target strain. The method and apparatus provided in this application enable efficient and accurate identification of nucleotide fragments that uniquely identify a specific target strain from massive amounts of genomic data, improving the accuracy, reliability, and efficiency of detecting specific target microorganisms.

The invention relates to the technical field of microbiological detection, in particular to a precise quantification method of lactobacillus helveticus CICC 6064 and application of the precise quantification method. The invention provides application of SEQ ID NO: 1 in precise quantification of a lactobacillus helveticus CICC 6064 strain, genome DNA of a to-be-detected strain is extracted, a primer pair (SEQ ID NO: 2 and SEQ ID NO: 3) and a TaqMan probe (SEQ ID NO: 8) are designed aiming at strain specific SNP sites on a nucleotide sequence shown in SEQ ID NO: 1, and in combination with a real-time fluorescent quantitative PCR technology, rapid, precise and quantitative detection of the strain on the strain level is realized. The method is suitable for quality control of samples such as food, daily chemicals and complex microbial inoculants added with the lactobacillus helveticus CICC 6064, and multi-dimensional application of a real-time monitoring technology is achieved.

The present application relates to the technical field of microorganism detection, and in particular to a precision quantification method of lactobacillus helveticus CICC 6064 and application thereof.The present application proposes the application of SEQ ID NO:1 in the precision quantification of lactobacillus helveticus CICC 6064 strain, extracts the genomic DNA of the strain to be detected, designs a primer pair (SEQ ID NO:2 and SEQ ID NO:3) and a TaqMan probe (SEQ ID NO:8) for the strain-specific SNP site on the nucleotide sequence shown in SEQ ID NO:1, and combines with real-time fluorescent quantification PCR technology to realize rapid and precise quantification detection of the strain at the strain level.The method of the present application is suitable for quality control of food, daily chemical, composite microbial agent and other samples added with lactobacillus helveticus CICC 6064, and realizes multidimensional application of real-time monitoring technology.

This invention belongs to the field of microbial engineering and wastewater treatment technology, specifically relating to a screening method for hydrolytic acidifying bacteria, a method for compounding bacterial agents, and their application. This invention involves screening hydrolytic acidifying bacteria from a sludge-water mixture in a hydrolysis acidification tank, constructing a combination of strains with synergistic degradation effects, preparing a compound bacterial agent, and applying it to wastewater with poor biodegradability. The main steps include: sample sourcing and treatment, initial strain screening, specific screening of functional strains, compounding of bacterial agents, application in hydrolysis acidification, and capacity testing. The screening and compounding method of this invention provides a reliable way to improve the biodegradability of recalcitrant wastewater and has promising prospects for water treatment applications.

The present application relates to the technical field of flexible materials, and particularly relates to a preparation method of a wireless passive biaxial strain sensor based on a nested double-loop antenna. The strain sensor designed and prepared by the method is composed of a nested double-loop antenna, the capacitance and inductance characteristics of the inner and outer loop structures under the tensile condition are regulated, two decoupled resonance frequencies are generated, and the x-axis and y-axis strain specificity sensitivities are respectively realized. Through laser scanning, an ink composed of liquid metal and styrene-ethylene / butylene-styrene copolymer (SEBS) two self-healing materials is activated, and the preparation of the stretchable biaxial strain sensor is realized. The circuit-free design realizes the bidirectional strain monitoring without crosstalk, and provides a new scheme for biomechanical sensors.