9 results about "Genetic divergence" patented technology

The invention discloses an SNP (Single Nucleotide Polymorphism) marker, a KASP primer and a detection method for purity identification of a cauliflower 'Youpon 68' hybrid. According to the method, on the basis of double-parent re-sequencing data of the Youpon 68 variety, an SNP site with stable genetic difference between parents is obtained through screening, and a KASP primer is designed according to the site. By utilizing the two SNP markers and combining a fluorescence detection technology, the male parent, the female parent and the hybrid variety of the variety can be quickly and accurately distinguished in a single reaction. Through early rapid identification of seed purity, production loss and market disputes caused by false hybrids can be effectively reduced, and identification efficiency and accuracy are significantly improved. The method has the characteristics of simplicity and convenience in operation, stable result, high flux and low cost, and has important application value in seed quality control, variety protection and breeding practice of the brassica oleracea L. 'Youpon 68'.

The invention belongs to the field of cassava molecular markers, and particularly relates to a cassava KASP marker and application of the cassava KASP marker in cassava variety distinguishing. The 27 KASP markers provided by the invention have good distinguishing ability in cassava genetic analysis, and can effectively reveal genetic differences among different materials. The DNA fingerprint spectrum of 184 parts of cassava materials is constructed based on 27 core KASP markers, and the purpose of rapid variety identification can be achieved. The KASP marker disclosed by the invention can be used for effectively identifying false selfing offspring. In conclusion, the KASP marker provided by the invention provides a new method and thought for genetic improvement and variety identification of cassava, provides a powerful tool for subsequent cassava breeding and genetic improvement research, and is beneficial to breeding of new varieties with excellent characters.

The invention discloses a method for constructing a plant gene fingerprint spectrum based on four different colors and application. Comprising the following steps: screening a plurality of genetic stable and representative gene loci according to genome information of a target species; genotype data is obtained by using technologies such as high-throughput sequencing, GBTS and SNP chips; four colors are set to correspond to different genotypes (such as homozygous dominant (AA), homozygous recessive (BB), heterozygous site (AB) and undetermined (UN)); genotype data is converted into a four-color fingerprint spectrum through a computer program to visually display genetic characteristics. According to the four-color coding scheme, color combinations can be set for different species to accurately reflect genetic differences. The method is suitable for multiple scenes such as crop breeding, germplasm resource management and genetic relationship analysis, and visual and reliable data support is provided for genetic improvement and resource protection.

The application discloses a method for grading and identifying silkworm germplasm resources. According to genetic differences in molecular evolution among silkworm germplasm resources, a grading and identifying method is established by using preferred specific SSR molecular markers, which can first distinguish different geographical populations, then distinguish germplasm resource groups in the same geographical population, and finally distinguish all silkworm germplasm resources in the same germplasm resource group. The method overcomes the problem that the prior art method cannot simultaneously establish specific DNA molecular markers for a large number of germplasm resources, and cannot accurately identify and distinguish specific germplasm resources.

The application discloses a japonica rice parent hybrid target gene plant screening method based on high-throughput sequencing and belongs to the field of agricultural biotechnology. The application uses high-throughput sequencing technology to accurately analyze the genetic differences of parent genomes, develops specific molecular markers covering target gene regions, can quickly identify plants containing target genes, greatly improves the screening efficiency and accuracy, shortens the breeding cycle and saves resources, combines molecular marker detection with the phenotypic performance of target traits, fixes target genes in each generation during the construction of hybrid and backcross populations, ensures the stable inheritance of target traits, and verifies the correlation between target genotypes and target traits and their environmental adaptability through joint analysis of genotypes and phenotypes and multi-environment testing, improves the stability and reliability of breeding materials, constructs excellent hybrid combinations and realizes genotype optimization in candidate plant screening and restorer line creation, and improves the quality and yield potential of breeding materials.

The invention relates to the technical field of oyster cross breeding, in particular to a COI genetic distance-based crassostrea calthrop hybridization prejudgment and combined configuration method. The method comprises the following steps: collecting COI gene sequences of calthrop oysters and four cultured oyster samples, constructing an allelic point sliding window, and calculating genetic differences by adopting a Kimura-2P distance, so as to obtain a refined COI genetic distance index; establishing a COI heredity-hybridization advantage regression model by combining historical hybridization survival data, heredity variation data and disturbance factors, introducing a multi-dimensional threshold structure based on genetic distance, and performing regression pre-judgment of threshold logic regulation and control on the hybridization advantage; on the basis, a COI genetic distance matrix is constructed, intraspecific fertility analysis and character complementation inference are coupled, and optimization and combined configuration of cross parents are achieved. Quantitative prediction of the hybridization advantage can be achieved before hybridization, the breeding period is shortened, the hybridization test cost is reduced, and the accuracy of breeding hybridization auxiliary decision making is remarkably improved.

This invention discloses a method for identifying the stipe length of Ganoderma lucidum using genetic differences, belonging to the field of Ganoderma lucidum breeding and cultivation technology. This method utilizes specific primer pairs del670 or insertion2441 to perform PCR amplification of Ganoderma lucidum mycelial DNA. Early prediction of the stipe phenotype is achieved by detecting the size of the amplified product fragments: if a 1569 bp fragment is obtained using the del670 primer pair or a 1357 bp fragment is obtained using the insertion2441 primer pair, the strain is identified as short-stalked; if an 899 bp fragment is obtained using the del670 primer pair or a 3798 bp fragment is obtained using the insertion2441 primer pair, the strain is identified as long-stalked. This method enables rapid and accurate identification of Ganoderma lucidum stipe length at the mycelial stage, eliminating the need for traditional fruiting cultivation, significantly shortening the breeding cycle and reducing testing costs.

The invention belongs to the technical field of molecular biology, and provides polymorphic primers of Chinese hippophae rhamnoides genome SSR molecular markers and application of the polymorphic primers. The SSR molecular marker is composed of 20 pairs of SSR molecular marker polymorphic primers, and the nucleotide sequences of the primers are as shown in SEQ ID NO.1-SEQ ID NO.40. The SSR molecular marker comprises 20 pairs of SSR molecular marker polymorphic primers. A large number of SSR sequences are mined by using transcriptomes, SSR capillary electrophoresis technology is combined, SSR loci with high measurement indexes Na value, I value and PIC value of genetic difference and front 20 of the sequence are screened, and Chinese sea-buckthorn molecular identity information is made and applied to Chinese sea-buckthorn geographic source identification. The SSR molecular marker is good in polymorphism primer specificity and high in polymorphism, and when the SSR molecular marker is applied to Chinese hippophae rhamnoides population genetic structure and resource genetic diversity analysis, the diversity and genetic structure of hippophae rhamnoides genetic level in China can be revealed more comprehensively, and a foundation is laid for formulating a genetic protection strategy.

The invention relates to the technical field of molecular markers, in particular to an SNP marker combination for buckwheat germplasm resource identification, a KASP primer combination and application. According to the method, deep sequencing is carried out on buckwheat core germplasm populations covering different ecotypes, geographic origins and genetic backgrounds, so that it is ensured that original data cover key variation regions of buckwheat genomes; sNP locus screening is carried out through multiple rounds of quality control standards, and finally a high-quality SNP marker set (namely the SNP marker combination provided by the invention) with high resolution and high credibility is obtained. The marker combination not only covers the uniform distribution area of the whole genome of buckwheat, but also can accurately capture subtle genetic differences among germplasm, effectively avoids identification deviation caused by a single marker or a small number of markers, and can accurately identify buckwheat germplasm in different sources.