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Method for identifying varieties of lilium brownii by adopting SSR (simple sequence repeat) molecular marking technology

A molecular marker and lily technology, applied in the direction of biochemical equipment and methods, microbial determination/inspection, etc.

Inactive Publication Date: 2015-01-07
KAILI UNIV
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Problems solved by technology

However, there are relatively few studies on the identification of lily germplasm, especially the use of SSR technology to identify the parents of lily varieties is rarely reported.

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  • Method for identifying varieties of lilium brownii by adopting SSR (simple sequence repeat) molecular marking technology
  • Method for identifying varieties of lilium brownii by adopting SSR (simple sequence repeat) molecular marking technology
  • Method for identifying varieties of lilium brownii by adopting SSR (simple sequence repeat) molecular marking technology

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Embodiment Construction

[0017] The present invention will be further described below in combination with specific embodiments.

[0018] The method for adopting SSR molecular marker technology of the present invention to identify lily varieties comprises the following steps:

[0019] 1. Prepare lily materials: Plant 11 kinds of lily materials, namely Shandanmeizi, Jieman, Nabi, Pink Angel, Perfume White, Solana, Black Monte, Purple Girl, Green Lily, Canada Yellow, and Guyana, in the gardening experiment The information of 11 kinds of lily varieties can be found in Table 1; at the seedling stage, 20 fresh leaves of each lily plant were randomly selected for use, frozen in liquid nitrogen and stored in a -70°C ultra-low temperature refrigerator for later use;

[0020] Table 1 Information of 11 lily varieties:

[0021]

[0022] 2. Lily DNA extraction: Extract the DNA of various lily materials with a plant genome kit, and use 1.5% agarose gel electrophoresis to detect the DNA purity; dilute the DNA co...

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Abstract

The invention relates to the method for identifying the varieties of lilium brownii by adopting an SSR (simple sequence repeat) molecular marking technology. A result shows that: two pairs of high-polymorphism and high-repeatability primers are screened from 13 pairs of primers for detecting 171 fragments comprising 131 polymorphic fragments, 4 to 22 fragments can be detected by each pair of primers with the sizes of 100 to 250bp, 1.69 bands are amplified by each pair of primers, and the polymorphism rate is 76.70 percent; NTSYS-pc2.11 is used for similarity coefficient and cluster analysis, the genetic similarity coefficient is 0.5882 to 09412, and the average similarity coefficient is 0.7647; materials are divided into six major classes by cluster analysis, the genetic differences between 11 lilium brownii germplasms are irrelevant to varietal characteristics, flower forms, the colors of filaments and styluses, but have certain correlation with the colors of petals, flower genes can better reflect genetic relationships between the lilium brownii germplasms, different varieties have different colors, and the primers can be designed by controlling related genes of flowers to provide an important means for further distinguishing the genetic relationships.

Description

technical field [0001] The invention relates to a plant variety identification method, in particular to a method for identifying lily varieties using SSR molecular marker technology. Background technique [0002] Lilium (Lilium brownine) is the general term for plants of the genus Lilium (lilium L.) in the family Liliaceae. China is one of the natural distribution centers of lily plants and has high edible, medicinal and ornamental values. At present, lily identification methods mainly include morphological, cytological, and biochemical markers, which are very common, which greatly affects the evaluation and utilization of lily germplasm resources in my country. The emergence of various molecular marker technologies is the rapid identification of lily germplasm. It provides a new technical method, and SSR markers have the advantages of covering the entire genome, high polymorphism, good repeatability and stability, and simple operation. It is currently one of the most suitabl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6895C12Q2600/156
Inventor 钟程田鑫李性苑刘伦沛杨苓
Owner KAILI UNIV
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