32 results about "Furtadoa" patented technology

The present invention relates to in planta transformation methods for plants or plant materials with a genetic construct, wherein at least one meristematic cell of a meristematic tissue of an immature inflorescence able to differentiate into a gamete of a pollen or of an ovule is exposed and then transformed, wherein the transformation can be performed to yield a stable integration or to yield a transient introduction of a genetic construct of interest. Further provided are methods for manufacturing a transgenic plant, or methods for manufacturing a genetically manipulated plant based on the in planta transformation methods according to the present invention. In addition, there is provided a plant or a progeny thereof manufactured according to the transformation and/or manufacturing methods according to the present invention.

The invention relates to the method for identifying the varieties of lilium brownii by adopting an SSR (simple sequence repeat) molecular marking technology. A result shows that: two pairs of high-polymorphism and high-repeatability primers are screened from 13 pairs of primers for detecting 171 fragments comprising 131 polymorphic fragments, 4 to 22 fragments can be detected by each pair of primers with the sizes of 100 to 250bp, 1.69 bands are amplified by each pair of primers, and the polymorphism rate is 76.70 percent; NTSYS-pc2.11 is used for similarity coefficient and cluster analysis, the genetic similarity coefficient is 0.5882 to 09412, and the average similarity coefficient is 0.7647; materials are divided into six major classes by cluster analysis, the genetic differences between 11 lilium brownii germplasms are irrelevant to varietal characteristics, flower forms, the colors of filaments and styluses, but have certain correlation with the colors of petals, flower genes can better reflect genetic relationships between the lilium brownii germplasms, different varieties have different colors, and the primers can be designed by controlling related genes of flowers to provide an important means for further distinguishing the genetic relationships.

The invention discloses a castanopsis hystrix pollen collecting and storing method for increasing the germination rate and improving the pollen viability. The method comprises the following steps that when male parent male flowers are in a transitional period of an early blooming period and a full blooming period, male parent male flower inflorescence is collected at 9:00-11:00 or 15:00-17:00; the male flower inflorescence is dried for 12-24 h in a sterile room at the temperature of 22 DEG C-28 DEG C under illumination of 80 W-160 W, when a large amount of anther blows out and a large amount of yellow pollen scatters during gentle shaking, drying is completed; the dried male flower inflorescence is screened with a pollen sieve, pollen is collected and contained in a container, an opening of the container is sealed, and the container is placed in an environment at the temperature of minue 60 DEG C-minus 100 DEG C or liquid nitrogen to be stored. Furthermore, when the pollen needs to be germinated, the stored pollen is placed in a liquid culture medium and can be germinated at the temperature of 25 DEG C-35 DEG C. Accordingly, a castanopsis hystrix pollen collecting, drying, storing and viability detecting system is formed for the first time, the method is simple, rapid and high in operability, the castanopsis hystrix pollen viability can be improved, and the manual cross pollination success rate of castanopsis hystrix can be increased.

The invention relates to plant genes and particularly discloses a novel symmetry gene CmCYC2c of chrysanthemum morifolium and a novel application of the symmetry gene CmCYC2c in the aspect of regulation of ligulate flower petal traits of the chrysanthemum morifolium, especially, in the aspects of increase of the number of ligulate flower petals and increase of petal length. The nucleotide sequence of the symmetry gene CmCYC2c is represented as SEQ ID NO.1. Based on the gene and the application thereof, the CmCYC2c gene is integrated to a chamomile genome with a transgenic technology, the flower type is primarily and directionally improved, so that the number of the ligulate flowers is increased, the petal length is increased, and a novel and practical method is provided for primary selection of new species of chrysanthemum morifolium varieties with the genetic engineering technology.

The invention discloses a planting and breeding combination ecological circulation method for a saccharum officinarum field, and belongs to the ecological agriculture field. The planting and breedingcombination ecological circulation method comprises the following steps of: (1) seed selection; (2) soil preparation and fertilizer application; (3) seeding conduction; (4) field management: a: carrying out reasonable fertilizer application, b: carrying out moisture management and c: thinning; (5) aquaculture: 1) carrying out drainage culture, and 2) carrying out moisture and food management; and(6) harvesting conduction. The planting and breeding combination ecological circulation method disclosed by the invention fully performs the advantages of the stereoscopic agriculture of the saccharumofficinarum field, a stereoscopic planting and breeding mode of the saccharum officinarum field is adopted, Cucumis sativus L. is planted among different pieces of saccharum officinarum, and fishes and Cipangopaludina chinensis Gray are cultured in grooves among furrows. Compared with a traditional saccharum officinarum cultivation way, the planting and breeding combination ecological circulationmethod has a better spatio-temporal allocation among species, and a colony ecological factor is further optimized.

The invention relates to an interspecific cross breeding method of fagopyrum dibotrys and tartary buckwheat and application. According to the interspecific cross breeding method of the fagopyrum dibotrys and the tartary buckwheat and application, with tetraploid tartary buckwheat as female parents and a diploid fagopyrum dibotrys complex plant doubled line and/or tetraploid fagopyrum dibotrys which naturally exists as male parents, crossing is conducted, and a cross breed obtained by adopting the interspecific cross breeding method of the fagopyrum dibotrys and the tartary buckwheat can live through the winter in warm southern areas, so that years of asexual propagating expanding production and cultivation are achieved. According to the breeding method, not only is the interspecific heterosis of the fagopyrum dibotrys and the tartary buckwheat used, but also the heterosis among different fagopyrum dibotrys lines is used, the perennial characteristic and the multiple regeneration capability of the interspecific cross breed fagopyrum dibotrys are further improved, and thus the regeneration capability of branch cutting and multiple cutting of cross breed plants is strong enough to support overwintering and propagating expanding of multiple branch cutting, so that the heterosis of the cross breed is maintained permanently, and the cross breed can be widely applied and popularized in production.

The invention relates to the field of rumen regulation and control, in particular to a natural product preparation for promoting the rumen fermentation. The natural product preparation is prepared through the following steps: after grinding homalomena occulta roots, extracting with 60-80 mass percent of ethanol solution, and performing nitrogen flushing and freeze drying to obtain dry powder. Through researching, the inventor has discovered that the homalomena occulta roots have a certain effect on promoting the rumen fermentation. Homalomena occulta has a wide source property and cooperativity, the experiment through adopting a filter paper flat band method shows that obscured homalomena rhizome volatile oil has a function of inhabiting bacterium burgeri on flocks and herds. Therefore, the homalomena occulta root extractive preparation is hopeful to further comprehensively improving the rumen function and promoting the health of ruminants after scientific compatibility.

The invention discloses a method for prolonging the refreshing time of borage flowers. The method comprises the following steps: (1) immersing the borage flowers into a sodium alginate solution, then taking out the borage flowers, and draining the residual sodium alginate solution; and (2) immersing the borage flowers into a calcium chloride solution to induce spontaneous cross-linking reaction, then taking out the borage flowers and absorbing moisture on the surfaces of the borage flowers. The preservation time of the borage flowers treated by the steps (1) and (2) is 5-6 days and is 4-5 days longer than the preservation time of untreated fresh cut flowers. The method comprises the step (3) that the borage flowers are inserted in a fresh-keeping solution, wherein the fresh-keeping solution is prepared from a bactericide, a nutritional agent, an ethene antagonist, a plant growth regulator and the like, so the aging of the borage flowers can be further slowed down, the vase life of the cut flowers can be prolonged, and the ornamental quality of the cut flowers can be improved. The preservation period of the borage flowers treated by the steps (1), (2) and (3) is 10-15 days and is 9-14 days longer than that of untreated fresh cut flowers, and a relatively high water activity value can be maintained.

The invention relates to the field of traditional Chinese medicine external application agents, and particularly discloses an external application agent for treating pelvic inflammation of cold uterustype and a preparation method thereof. The external application agent for treating the pelvic inflammation of the cold uterus type is endowed with the effects of relieving pain and diminishing inflammation in virtue of mutual compatibility of radix angelicae dahuricae, radix dipsaci asperoidis, zanthoxylum schinifolium, ligusticum chuanxiong, cortex acanthopanacis, homalomena rhizome and trberculate speranskia herb, so that the external application agent is capable of effectively diminishing inflammation and removing swelling; the external application agent is endowed with the effects of dispelling wind and eliminating dampness in virtue of mutual compatibility of the zanthoxylum schinifolium, the homalomena rhizome, cortex illicii, Chinese viscum herb, argy wormwood leaves, the trberculate speranskia herb, incised notopterygium rhizome and roots, double-teeth pubescent angelica roots and the ligusticum chuanxiong, so that the external application agent is capable of effectively removing coldness and dampness from the uterus of the patient; and the external application agent is also endowed with the effects of promoting blood circulation for regulating menstruation in virtue of mutual compatibility of the argy wormwood leaves, the trberculate speranskia herb, radix paeoniae rubra, Chinese angelica roots, frankincense, myrrh, common buried rubber, zedoray rhizome and red-rootedsalvia roots, so that the external application agent is capable of effectively promoting blood circulation for regulating menstruation of the patient. Moreover, in virtue of the effects of an absorption enhancer, a ginger brown-sugar preparation and a scallion stalk extract, the external application agent becomes further capable of preventing and treating bacteria in the uterus of the patient.

The invention relates to a medicine composition with a special effect of tonifying yang. The medicine composition is prepared from the following components in parts by mass: 110-130 parts of radix paeoniae alba, 50-70 parts of radix astragali seu hedysari, 40-50 parts of rhizoma drynariae, 40-50 parts of caulis spatholobi, 20-30 parts of herba epimedii, 20-30 parts of radix morindae officinalis, 20-30 parts of radix clematidis, 20-30 parts of herba lycopodii, 20-30 parts of herba speranskiae tuberculatae, 20-30 parts of radix angelicae sinensis, 20-30 parts of rhizoma homalomenae, 15-25 parts of radix glycyrrhizae preparata and 25-35 parts of centipede. According to the medicine composition, radix paeoniae alba, radix astragali, rhizoma drynariae, caulis spatholobi, herba epimedii, radix morindae officinalis and radix clematidis are taken as main medicines, and the seven main medicines are synergistically matched, so that the symptoms such as lumbago, lumbar muscle strain, cramp of legs and feet, flaccid paralysis, rheumatic arthralgia and tendon and vessel spasm are effectively improved, and a yang-tonifying medicinal effect is provided to improve various yang deficiency symptoms; meanwhile, the lycopodium clavatum, the garden balsam stem, the angelica sinensis and the obscured homalomena rhizome are used as ministerial drugs, so that the effects of inducing diuresis, detoxifying, stimulating the menstrual flow, penetrating the stem, enriching and promoting blood circulation and the like are achieved; and finally, honey-fried licorice root and centipede are used as adjuvants, and the detoxification function of the adjuvants is utilized, so that damage to liver and kidney caused by long-term administration is avoided.

The invention specifically relates to a flowerpot capable of sufficient watering. The flowerpot comprises a flowerpot body with an opening in the upper end, wherein a jacket is arranged outside the flowerpot body; a water filling hole is formed in the jacket; a water storage cavity is formed in the jacket; and a water inlet hole communicating with the water storage cavity is formed in the side wall of the flowerpot body. By means of the arranged jacket, water can enter the flowerpot body from the middle lower portion of the flowerpot body, so that sufficient irrigation of roots of plants is guaranteed, loss of soil on the surface is also avoided, and the air permeability of the flowerpot body can be greatly improved through a further arranged vent pipe.

The invention discloses a method for increasing the weight of Feizixiao lychees per fruit. The method includes the following steps: S1, after the Feizixiao lychees have spikes in early February, manually thinning flowers, only retaining one spike at the root of each flower, and removing residual spikes; S2, covering the spikes with a plastic greenhouse, and keeping the internal temperature of thegreenhouse at 25-27 DEG C, wherein the growth and development of the spikes are promoted through temperature rise in the greenhouse, and all female flowers of the lychee spikes are promoted to flowerin late February; and S3, in the flowering stage, applying bee-assisted pollination, removing the plastic greenhouse after pollination and fertilization, and allowing fruits to grow and develop in a natural state until ripening. The method has the advantages that through a high-temperature environment in the greenhouse, development of lychee buds and early flowering are promoted; after pollinationand fertilization, the fruits further develop and ripen under a natural-temperature environment, and accordingly, the weight of the lychees per fruit is increased.

The invention provides a preparation process of coptis chinensis flower dish. The preparation process comprises following steps: step A, pre-processing: fresh coptis chinensis flowers at the scape growing and flowering stage, and impurities on the coptis chinensis flowers are removed firstly for standby application; step B, fixation: tools include a stainless steel pot, a bamboo-strip or stainlesssteel scoop, table salt and a plastic basin, the table salt is added when clear water is heated to 95 DEG C or higher, a ratio of water to the salt is 1000:4, meanwhile, the standby flowers are put in the stainless steel pot with boiling water and are heated and cooked for 5-6 min, the bamboo-strip or stainless steel scoop is utilized to stir the flowers continuously, then the flowers are taken out and placed in a stainless steel container or the plastic basin filled with cold water for first cooling; and step C, cooling: during cooling, sufficient clear water is needed, the flowers are needed to be turned in time, uniform cooling is ensured, and the flowers are subjected to secondary rinsing after about 10 min. By means of packaging, the use value of the coptis chinensis flowers can be further improved, and the prepared coptis chinensis flower dish is relatively crisp, tasty and delicious.

The invention relates to the technical field of anthurium flower planting, in particular to a retractable anthurium flower planting support. The retractable anthurium flower planting support comprisesa plurality of longitudinal containing frames symmetrically arranged at the two sides of a water distribution and temperature control box, so that water distribution and heat preservation of anthurium flowers in the containing frames at the two sides are achieved; the distance between the containing frames at the two sides and the water distribution and temperature control box can be adjusted through a contraction motor, longitudinal three-dimensional space is comprehensively utilized, and the problems that traditional anthurium flowers are subjected to spraying and temperature control from the upper portion, consequently, the longitudinal three-dimensional space is wasted and cannot be comprehensively utilized is solved; and an autorotation motor worm arranged on a connecting frame is matched with a worm wheel, so that each containing frame can autorotate along the worm wheel on the basis of fully utilizing the three-dimensional space in the longitudinal direction, and the water distribution and temperature control box can perform uniform spraying and temperature control effects on the anthurium flowers placed in the containing frames in the circumferential direction, and the problems that the anthurium flowers are placed in a three-dimensional manner, spraying is uneven, and the appearance of the anthurium flowers is affected are further solved.

The invention provides a method for controlling tetranychus cinnabarinus and relates to the technical field of biological control for the tetranychus cinnabarinus. According to the method, through combined action of creeping oxalis and chrysopa sinica, the tetranychus cinnabarinus of garden plants are biologically controlled. The creeping oxalis can massively trap the tetranychus cinnabarinus, andthe potted creeping oxalis which has trapped the tetranychus cinnabarinus can be further used as a natural enemy source feeding nursery of chrysopa sinica adults, cannot pollute the environment and is safe to natural enemies; the biological diversity of an ecological system is improved; as the feeding nursery of the natural enemies, the potted creeping oxalis can promote natural control for the natural enemies, so that use of insecticides is substantially reduced, the cost is saved and the benefits are increased. When the method is used for controlling the tetranychus cinnabarinus, particularly, when the flower watching stage of the creeping oxalis and the spawning period of the tetranychus cinnabarinus coincide, a best trapping effect on the tetranychus cinnabarinus on the garden plantscan be realized, and a better control effect on the tetranychus cinnabarinus can be realized.

The invention belongs to the technical field of ecological engineering, and discloses a comprehensive method for treating spartina alterniflora through fresh water submerging. Physical prevention and treatment comprises the steps of water submerging, cutting, ploughing, root smashing, and covering and shading; chemical prevention and treatment comprises the step of preparing a herbicide for spartina alterniflora treatment; biological prevention and treatment comprises the step of reducing the density of the spartina alterniflora to realize the recovery of a mud flat ecological system; and biological substitution comprises the step of according to a plant community succession rule, replacing alien invasive plants with local plants with strong competitiveness. Spartina alterniflora prevention and control research is carried out in the method, the spartina alterniflora is managed in a combined mode of fresh water submerging, ploughing and biological mulching film covering, the original ecology can be restored while expansion of the spartina alterniflora is controlled, a comprehensive spartina alterniflora prevention and control demonstration base is established in the Yellow sea mud flat, and the prevention and control mode of the spartina alterniflora is further popularized; and a wetland vegetation habitat is restored in an implementation area, the wetland ecological function is improved, waterfowl is successfully attracted for inhabitation, and thousands of gray cranes and bean geese are found in an experimental area to find food and inhabit towards the north.

The invention belongs to the technical field of biology, and discloses application of metallothionein DaMT2a and a coding gene thereof. Full-length cDNA of the metallothionein DaMT2a gene of Dioscoreaalata L. is cloned for the first time; gene expression analysis shows that the DaMT2a gene is highly expressed in male flowers and stems, expression is up-regulated under the induction of ethephon and abscisic acid, meanwhile expression of the gene is up-regulated under the induction of low temperature and mechanical injury stress, and expression is down-regulated under high temperature stress, so that the gene is possibly closely related to stress resistance of the Dioscorea alata L., and the gene can be used as an important target gene for transgenic breeding of the Dioscorea alata L.. Thegene is further transferred into a prokaryotic expression strain, and the growth of a transgenic strain under normal conditions, heavy metal ions (Cu<2+> and Cd<2+>), NaCl stress and hydrogen peroxidestress is obviously improved, so that the gene can be used as an important gene resource, and the gene can be applied to stress resistance and heavy metal stress resistance gene engineering of otherplants or microorganisms except for the Dioscorea alata L..

The invention discloses an SCAR molecular marker for identifying petal splinter character of a tagetes erecta ligulate flower, a detection primer and application thereof. The nucleotide sequence of the SCAR molecular marker in the non-splinter of the tagetes erecta ligulate petals is shown as SEQ ID NO.1, and the nucleotide sequence of the SCAR molecular marker in the splinter of the tagetes erecta ligulate petals is shown as SEQ ID NO.2. The invention further provides a detection primer and a detection kit for identifying the petal splintercharacterof the tagetes erecta ligulate flower. The SCAR molecular marker provided by the invention can accurately and efficiently distinguish whether the ligulate flower petal splinters of the tagetes erecta exist or not, the detection efficiency of the SCAR molecular marker in an F2 segregation population with the ligulate flower petal splinters of the tagetes erecta can reach 96.12%, the character with the ligulate flower petal splinters of the tagetes erecta can be quickly screened by directly utilizing a PCR product, the tagetes erecta can be selected in the seedling stage, the breeding efficiency is obviously improved, and the breeding cost is reduced.

The invention discloses a breeding method of a new variety of large-flower petunia hybrida. The breeding method comprises the following steps: multiple generations of selfing homozygous petunia hybrida are selected as parents, at least one of the male parent or the female parent is a large-flower selfing line, the flower diameter is 8.0 cm or above, the other parent is a middle-flower or large-flower selfing line, and the flower diameter is 4.0 cm or above; in the initial flowering stage of the parents, castration is conducted on the female parent, then pollen of the male parent is smeared on pistil stigma of the female parent, and cross pollination is completed; after the pods are mature, hybrid F1 seeds are collected before the pods crack; and flowers of the F1 plants obtained through sowing and cultivation are all in a large flower shape. The breeding method of the new variety of the large-flower petunia hybrida is established for the first time, an effective way is provided for breeding of the new variety of the large-flower petunia hybrida, a foundation is laid for further enriching garden flower germplasm resources and improving the ornamental effect of the petunia hybrida landscaping application, and the method has practical application value and wide application prospects.